JPS60173465A - 新規なアフイニテイクロマトグラフイ用吸着体 - Google Patents

新規なアフイニテイクロマトグラフイ用吸着体

Info

Publication number
JPS60173465A
JPS60173465A JP59029935A JP2993584A JPS60173465A JP S60173465 A JPS60173465 A JP S60173465A JP 59029935 A JP59029935 A JP 59029935A JP 2993584 A JP2993584 A JP 2993584A JP S60173465 A JPS60173465 A JP S60173465A
Authority
JP
Japan
Prior art keywords
adsorbent
alpha
amino acid
affinity chromatography
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP59029935A
Other languages
English (en)
Japanese (ja)
Other versions
JPH05990B2 (enrdf_load_stackoverflow
Inventor
Akira Tai
田井　晰
Hiroshi Ozaki
宏 尾崎
Tsutomu Masuda
勉 増田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP59029935A priority Critical patent/JPS60173465A/ja
Publication of JPS60173465A publication Critical patent/JPS60173465A/ja
Publication of JPH05990B2 publication Critical patent/JPH05990B2/ja
Granted legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP59029935A 1984-02-20 1984-02-20 新規なアフイニテイクロマトグラフイ用吸着体 Granted JPS60173465A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP59029935A JPS60173465A (ja) 1984-02-20 1984-02-20 新規なアフイニテイクロマトグラフイ用吸着体

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59029935A JPS60173465A (ja) 1984-02-20 1984-02-20 新規なアフイニテイクロマトグラフイ用吸着体

Publications (2)

Publication Number Publication Date
JPS60173465A true JPS60173465A (ja) 1985-09-06
JPH05990B2 JPH05990B2 (enrdf_load_stackoverflow) 1993-01-07

Family

ID=12289841

Family Applications (1)

Application Number Title Priority Date Filing Date
JP59029935A Granted JPS60173465A (ja) 1984-02-20 1984-02-20 新規なアフイニテイクロマトグラフイ用吸着体

Country Status (1)

Country Link
JP (1) JPS60173465A (enrdf_load_stackoverflow)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104399283A (zh) * 2014-12-04 2015-03-11 江西省农业科学院农产品质量安全与标准研究所 黄曲霉毒素b1适配体亲和柱的制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104399283A (zh) * 2014-12-04 2015-03-11 江西省农业科学院农产品质量安全与标准研究所 黄曲霉毒素b1适配体亲和柱的制备方法
CN104399283B (zh) * 2014-12-04 2016-02-17 江西省农业科学院农产品质量安全与标准研究所 黄曲霉毒素b1适配体亲和柱的制备方法

Also Published As

Publication number Publication date
JPH05990B2 (enrdf_load_stackoverflow) 1993-01-07

Similar Documents

Publication Publication Date Title
Plummer Jr et al. Human plasma carboxypeptidase N. Isolation and characterization.
Kimmel et al. The properties of papain
Pantoliano et al. Affinity chromatographic purification of angiotensin converting enzyme
JPS5839513B2 (ja) 精製したトリプシンおよびトリプシン様酵素の貯蔵法
Bazzone et al. Single-step isolation and resolution of pancreatic carboxypeptidases A and B
Mitz et al. Isolation of proteolytic enzymes from solution as dry stable derivatives of cellulosic ion exchangers
Uren Affinity chromatography of proteolytic enzymes
US4252902A (en) Process for purification of crude kallikrein
JPS60173465A (ja) 新規なアフイニテイクロマトグラフイ用吸着体
Pass et al. The Use of Affinity Chromatography in Determining the Sites of Protein‐Protein Interaction Relative to the Binding Sites of Substrates in Gramicidin S Synthetase
US4708944A (en) Protease adsorbent and process for purifying TPA utilizing the same
Ishii et al. [64] Affinity methods using argininal derivatives
Pacaud Purification of protease II from Escherichia coli by affinity chromatography and separation of two enzyme species from cells harvested at late log phase
Brisbane et al. Gas chromatographic analysis of amino acids from the action of proteolytic enzymes on soil humic acids
US4100028A (en) Method for purification of proteolytic enzymes
NARITA et al. Rat liver enzyme does not activate acetylamino acids
NO168218B (no) Elektrisk hengeisolator
SOMENO et al. Affinity chromatography of urokinase on an agarose derivative coupled with pyroglutamyl-lysyl-leucyl-argininal
JPH0147997B2 (enrdf_load_stackoverflow)
US7365173B2 (en) Method for the production of pure virally inactivated butyrylcholinesterase
Allison et al. Evidence for direct hydrogen transfer during glyceraldehyde-3-phosphate dehydrogenase catalysis
JPH0244510B2 (enrdf_load_stackoverflow)
Cherkasov Affinity chromatography of enzymes
JP2551399B2 (ja) Ｃ末端アミド化に関与する酵素並びにその製造方法及びその使用
JPS5838150B2 (ja) カリクレインの精製法