JPS60149369A - Mycelial extract of ganoderma lucidum and production thereof - Google Patents

Abstract

PURPOSE:To obtain the titled extract containing drug effect components and nutritious components, e.g. anticancer, sedative and analgesic components, in a high concentration, by inoculating fungi of Ganoderma lucidum into a solid culture medium containing bagasse as a base material, cultivating the fungi, disintegrating the culture medium, adding water thereto, and pulverizing and milling the bagasse fibers under heating. CONSTITUTION:Fungi of Ganoderma lucidum are inoculated into a solid culture medium containing bagasse as a base material to propagate the mycelia of the Ganoderma lucidum. The resultant culture medium containing the mycelia is then disintegrated to give preferably <=30wt% portion passing through a 12- mesh screen. Water in an amount of preferably 2-6 times that of the disintegrated culture medium is added thereto, and the bagasse fibers are pulverized and milled while heating the culture medium at <=95 deg.C to give preferably >=70 wt% bagasse fibers passing through the 12-mesh screen. Thus, the aimed extract is obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION Technical Field of the Invention The present invention relates to a Ganoderma mycelium extract and a method for producing the same. More specifically, the present invention relates to a Ganoderma mycelium extract extracted from Ganoderma mycelium grown in a solid medium based on Bacchus, and a method for producing the same.

Technical background of the invention and its problems Mushrooms have been widely used for food for many years, and some types of mushrooms have also been used for medicinal purposes. Mushrooms consist of a fruiting body, which is usually used for food or medicine, and a mycelium, which corresponds to the stem or root of the mushroom. It functions as a trophic organ that supplies nutrients. As described above, the fruiting bodies of mushrooms have been widely used for food and medicinal purposes, but in mushrooms such as shiitake, it is said that more nutritional and medicinal ingredients are contained in the mycelium than in the fruiting bodies. It has only been discovered in recent years. For example, in the case of shiitake mushrooms, the essential amino acid threonine is contained in only 0.80g in 100g of fruiting body, but 2.07g (l) in mycelium 1009.
It is also reported that it includes

Under these circumstances, attempts have been made to extract medicinal or nutritionally valuable active ingredients from shiitake mushroom mycelium. For example, Tokuko Sho 51-1'9', 0
Publication No. 13 discloses that after inoculating shiitake mushroom seeds into a solid medium made by adding rice bran or the like to sawdust and growing mycelium by a conventional method, the medium immediately before or after the fruiting body is pulverized and water is added. , adjust liH to 5°0, seal it in a container, and heat it to 30~5.
From solid cultured shiitake mushroom mycelium, which is heated to 5°C to promote the metabolism of mycelial enzymes and metabolites, and after the enzymatic reaction is sufficiently carried out, this suspension is filtered. A method for extracting medicinal ingredients is disclosed. In addition, in Japanese Patent Publication No. 53-23.392, Shii R bacteria is inoculated into a solid medium made of fallen epidermis or Bacchus as a base material and rice bran is added as needed, and mycelium is grown. After growth, the medium containing the mycelium is crushed and pH-adjusted water is added, sealed in a container and heated to 30-55°C to promote the metabolism of the mycelium and to initiate the enzymatic reaction. A method for producing a health drink is disclosed, which is characterized in that the hot liquid obtained is filtered after sufficiently carrying out the above steps.

However, the methods disclosed in the above publications all use shiitake mushroom mycelium as a raw material, and
The method disclosed in Publication No. 13 has the disadvantage that lignin, tannin, etc. contained in sawdust are transferred and contained in the obtained liquid, so it has a strong bitter taste and is unsuitable for use as a beverage. Ta. In addition, the special public interest public interest rate was 392 February 3, 1973.
In the method disclosed in the publication, when extracting the active ingredient from a solid medium containing shiitake mushroom mycelium, it is necessary to make the following adjustments: 1) In addition, it is necessary to maintain the humidity at 30 to 55 °C for a long time. As a result, process control is complicated and time-consuming.

On the other hand, Reishi mushroom belongs to the genus Prunus spp. and is generally referred to as ganoderma, and has been known as a Chinese herbal medicine with extremely excellent medicinal effects since ancient times.

According to the ``One Plant Class Index'' written by Li Shizhen, a medical scientist and pharmacologist of the Ming Dynasty in China, six types of reishi are known: green grass, red grass, yellow grass, white grass, black grass, and purple grass, and in recent years. Reishi mushrooms have been shown to have anti-cancer effects, as well as sedative, analgesic, and antitussive effects, as well as excellent medicinal effects on the cardiovascular system, including hypertension.

As described above, Reishi mushroom has excellent medicinal properties, but until now, the fruiting body of Reishi mushroom was either boiled and drunk, or the fruiting body was finely ground and eaten. As a result of further in-depth research into the medicinal and nutritional components of Reishi, the inventor discovered that even in Reishi, more medicinal and nutritional components are contained in the mycelium than in the fruiting body. Through further research, they discovered a method for effectively extracting medicinal and nutritional components from Ganoderma mycelium, and completed the present invention.

OBJECTIVES AND SUMMARY OF THE INVENTION The present invention provides a Ganoderma mycelium extract containing extremely high concentrations of medicinal and nutritional components, and also provides a method for producing the Ganoderma mycelium extract. .

The Ganoderma mycelium extract according to the present invention can be obtained by extraction from a Bacas base medium containing Ganoderma mycelium. In addition, the method for producing Ganoderma mycelium extract according to the present invention involves inoculating Reishi fungi into a solid medium based on Bacchus, propagating Ganoderma mycelium, and unraveling the obtained mycelium-containing solid medium. ,
It is characterized by adding water and then crushing the Bacas fibers while heating to a humidity of up to 95°C.

The Ganoderma mycelium extract according to the present invention contains saccharides.

Ergosterine, mannitol, adenine, uracil,
Glycine betaine, stearic acid, polynureosytate,
It contains high concentrations of medicinal ingredients such as polyamino acids and interferon derivatives as well as nutritional ingredients. on the other hand,
When the Ganoderma fruiting body is extracted with water, the concentration of the above-mentioned medicinal and nutritional components is not as high as when the Ganoderma mycelium is extracted with water.

Furthermore, since the Ganoderma mycelium extract according to the present invention is extracted from a Bacas base medium containing Ganoderma mycelium, it is free from sugars and proteins contained in Bacas.

-Active ingredients such as active ingredients are also extracted at the same time, and the nutritional and medicinal ingredients in the bakasu are also contained in sufficiently high concentrations.

As the base material of the solid medium in the present invention, Bacas or Bacas to which the dried leaf diameter of sugar cane is added, or Bacas to which rice bran is added is used. Bakas is the squeezed residue of sugarcane, and contains sugars and proteins that serve as nutritional sources for mycelium.
Although it can be used as a solid medium as it is, it can also be made into a solid medium by adding 10 to 30 parts by weight of rice bran to 100 parts by weight of bakasu. Furthermore, minerals such as phosphorus, ul, germanium, etc. can be added to the Bacchus medium as necessary.

Reishi bacteria to be inoculated into a solid medium based on Bakasu include green grass, red grass, yellow grass, white grass, black grass, and purple grass. is not limited to. After the Reishi fungus is inoculated onto solid j8 soil, humidity and i! ii! The mycelium is left for a predetermined period of time in a culture chamber with regulated illuminance to allow the mycelium to grow.

After the Ganoderma mycelium has sufficiently grown and the mycelium has sufficiently spread in the medium, the 41 fibers of the Bass medium are untied, preferably 12 meshes, immediately before or after the emergence of fruiting bodies. The amount passed through should be 30% by weight or less. When unbundling this Bakasu cellulose, the amount passing through 12 meshes is
=mJn% or more requires a special grinder, which is not preferable because the active ingredient may be crushed.

In other words, when the Bacchus culture medium is unbundled without using a special pulverizer or the like, the 12-mesh unbundled content is 30% by weight or less. Incidentally, it is preferable to unbundle the Bacchus substrate medium immediately after the fruiting body emerges or immediately after the fruiting body emerges, but depending on the case (in some cases, it may be done after the fruiting body has grown considerably).

Bacas medium I
Kg to 1-10Kg, preferably 2-6! ] of water is added. There is no need to adjust the pH of this added water. At this time, in addition to water, enzymes such as cellulase, protease, glucosylase, alcohols such as ethyl alcohol, etc. may be added as necessary.

Next, the suspension consisting of the Bacas medium and water prepared as described above is ground and moistened so that preferably at least 70% by weight of the Pacas fibers pass through 12 meshes. This crushing and crushing may be carried out while maintaining the humidity at 30 to 50°C, but during the crushing and crushing process, the temperature may be raised above the above temperature to slightly improve the quality. It is preferable to do it slowly.

The Bacchus 41 fibers are crushed and crushed by circulating the Bacchus fiber-containing mixture using a variable speed gear pump or the like.
However, this can be done by applying crushing and crushing action to the Bacchus 41i fiber in a portion of gear A7. J. While circulating the solid medium-containing mixture using a pump, a fine crusher was installed separately, and this sowing was performed using a pump.
The solid medium may be heated by heating. In the heating process, when water) B reaches 60°C, preferably 70'C or higher, when air at room temperature is injected into the suspension, the air bubbles are heated to the core and cause a destruction phenomenon. For this reason, it is possible to impact the Bacchus fibers and extract the active ingredients more effectively. It is preferable to repeat this operation several times.

Shatter and! It is preferred that at least 70% by weight of the crushed bakasu fibers pass through 12 meshes. If the amount passing through the mesh is less than 70% by weight, the effective ingredients in the solid medium will not be extracted sufficiently, and there will be a large portion where the cellulose will not be softened sufficiently, and the solid residue obtained by 1 q will be converted into feed. Otherwise, it is undesirable because it cannot be used effectively as fertilizer. These minute suspended matter are not only decayed matter from the medium, but also proteins and precipitates solidified by enzymatic reactions and heating. This microfloat itself has a nutritional value of 2, but also has the effect of improving the mouthfeel when drinking it. If the presence of floating matter is a concern, should the floating matter be allowed to settle and be separated?

Alternatively, by using a piece of cloth etc.
'11-5 and can be taken as is, or in some cases, excipients may be added to the extract to form tablets. Furthermore, it may be made into granules by freeze-drying.

Reishi mycelium obtained by the present invention is a liquid obtained by decoction of Reishi fruiting bodies or a powder obtained by crushing Reishi fruiting bodies. It has excellent medicinal properties that were previously thought to be possessed by Reishi mushrooms. The extract of Ganoderma mycelium thus obtained contains 1, saccharides, ercoderin, mannyl, adenine, uracil, glycine betaine, stearic acid, polynucleoside, polyamino acids, and interferon 1.
Contains high concentrations of major components such as carbon derivatives.

Effects of the Invention In the present invention, Ganoderma mycelium is propagated in a Bacchus medium, and water is added to this to extract the active ingredient from the Ganoderma mycelium, so it has the following effects.

(a) The active ingredient can be extracted at an extremely high concentration compared to the case where the active ingredient is extracted from the fruiting bodies of Reishi mushrooms.

(b) Efficiently extract medicinal ingredients or nutritional fIII7t ingredients from Bakasu j8 which contains Reishi fruiting bodies.
It can be extracted without H adjustment.

(C) The Bacas culture medium left after the active ingredients have been extracted is sufficiently finely and softened by enzymes and crushing/seeding action, so that it can be used as fertilizer, feed, or food.

Hereinafter, the present invention will be explained in detail with reference to Examples, but the present invention is not limited to these Examples.

Example 1 A solid medium consisting of 90 parts by weight of Bakasu and 10 parts by weight of rice bran was appropriately impregnated with pure water, and then inoculated with Ganoderma lucidum and left in a culture chamber under Condition V and humidity. Made it multiply. Just before the mycelium spreads on the solid medium and the fruiting bodies appear,
The fiber material of the Vacas base material was unbundled so that the amount passing through 12 meshes was 24% by weight or less. This unbundled medium 1. Pure water at 3.5°C was added to OK(+) while keeping the temperature at 40°C to prepare a mixture containing Bacas.

Next, while circulating the culture medium-containing mixture using a gear pump with variable speed, the solid medium was subjected to crushing and dish crushing action for about 200 minutes in a portion of Gear A7, resulting in about 80% of the Bacas fibers.
Sato% was set to 12 meshes passing through. The crushing and crushing of the Bakasu-containing mixture was carried out while gradually increasing the degree of flatness of the mixture.

Every time the water temperature reached about 70°C, the crushing and crushing of the Bacchus fibers was stopped, and the pump was rotated in the opposite direction to inject air bubbles into the suspension. The air bubbles were rapidly heated, causing a destruction phenomenon, and the Bakasu fibers were sufficiently stirred in the suspension.

Thereafter, the mixture (containing Bacas) was gently heated to 90° C. and left for 30 minutes. The enzyme was inactivated and sterilized by heating to 90°C. The obtained culture medium-containing mixture was filtered using a 60-mesh filter to obtain a health drink containing microfloated particles.

On the other hand, the solid residue can be obtained by sufficiently finely pulverizing it.
After drying, it was provided as feed for livestock such as cows.

Claims (2)

[Claims] (1) Reishi mycelium extract extracted from Bacas base medium containing Reishi mycelium. (2) A method for producing a Ganoderma mycelium extract, which comprises applying a Reishi tube to a solid medium based on Bacas and heating it to a temperature of up to 5° C. while crushing and rolling Bacas fibers.