JPS5972040A - 電子顕微鏡観察用高分子試料の調製方法 - Google Patents
電子顕微鏡観察用高分子試料の調製方法Info
- Publication number
- JPS5972040A JPS5972040A JP57181392A JP18139282A JPS5972040A JP S5972040 A JPS5972040 A JP S5972040A JP 57181392 A JP57181392 A JP 57181392A JP 18139282 A JP18139282 A JP 18139282A JP S5972040 A JPS5972040 A JP S5972040A
- Authority
- JP
- Japan
- Prior art keywords
- contrast
- electron microscopy
- phase
- osmium tetroxide
- tetroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 17
- 229920000642 polymer Polymers 0.000 title claims description 8
- 238000001493 electron microscopy Methods 0.000 title claims description 7
- 229910000489 osmium tetroxide Inorganic materials 0.000 claims description 17
- 239000012285 osmium tetroxide Substances 0.000 claims description 17
- 229910001927 ruthenium tetroxide Inorganic materials 0.000 claims description 12
- 230000005855 radiation Effects 0.000 claims description 11
- 238000010186 staining Methods 0.000 claims description 9
- 239000002861 polymer material Substances 0.000 claims description 7
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 description 14
- 239000000463 material Substances 0.000 description 11
- 238000010894 electron beam technology Methods 0.000 description 9
- 238000004627 transmission electron microscopy Methods 0.000 description 9
- 239000000523 sample Substances 0.000 description 8
- 229920001577 copolymer Polymers 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- -1 polybutylene terephthalate Polymers 0.000 description 6
- 239000002245 particle Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N ethylene glycol Natural products OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 3
- 229920000098 polyolefin Polymers 0.000 description 3
- 230000009257 reactivity Effects 0.000 description 3
- 238000007447 staining method Methods 0.000 description 3
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 229920000554 ionomer Polymers 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229920001748 polybutylene Polymers 0.000 description 2
- 229920001707 polybutylene terephthalate Polymers 0.000 description 2
- 229910052707 ruthenium Inorganic materials 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229920000800 acrylic rubber Polymers 0.000 description 1
- 229920001893 acrylonitrile styrene Polymers 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 125000001033 ether group Chemical group 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- SCUZVMOVTVSBLE-UHFFFAOYSA-N prop-2-enenitrile;styrene Chemical compound C=CC#N.C=CC1=CC=CC=C1 SCUZVMOVTVSBLE-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57181392A JPS5972040A (ja) | 1982-10-18 | 1982-10-18 | 電子顕微鏡観察用高分子試料の調製方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57181392A JPS5972040A (ja) | 1982-10-18 | 1982-10-18 | 電子顕微鏡観察用高分子試料の調製方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5972040A true JPS5972040A (ja) | 1984-04-23 |
| JPH0225447B2 JPH0225447B2 (OSRAM) | 1990-06-04 |
Family
ID=16099935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57181392A Granted JPS5972040A (ja) | 1982-10-18 | 1982-10-18 | 電子顕微鏡観察用高分子試料の調製方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5972040A (OSRAM) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036612C (zh) * | 1994-03-08 | 1997-12-03 | 中国人民解放军第304医院 | 透射电子显微镜生物样品超薄切片染色液 |
| JP2013209647A (ja) * | 2012-03-29 | 2013-10-10 | Xerox Corp | ポリマー表面の化学的な表面安定化プロセス |
| JP2013224927A (ja) * | 2012-03-23 | 2013-10-31 | Sumika Chemical Analysis Service Ltd | 観察試料、観察試料の作製方法、及び観察方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3922064B2 (ja) * | 2002-03-25 | 2007-05-30 | 松下電器産業株式会社 | 電池極板中の結着剤の分散状態を評価する方法 |
-
1982
- 1982-10-18 JP JP57181392A patent/JPS5972040A/ja active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036612C (zh) * | 1994-03-08 | 1997-12-03 | 中国人民解放军第304医院 | 透射电子显微镜生物样品超薄切片染色液 |
| JP2013224927A (ja) * | 2012-03-23 | 2013-10-31 | Sumika Chemical Analysis Service Ltd | 観察試料、観察試料の作製方法、及び観察方法 |
| JP2013209647A (ja) * | 2012-03-29 | 2013-10-10 | Xerox Corp | ポリマー表面の化学的な表面安定化プロセス |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0225447B2 (OSRAM) | 1990-06-04 |
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