JPS5971651A - Feed for fish farming - Google Patents

Abstract

PURPOSE:To promote palatability and growth of fishes and Crustacea, by adding a digestion extract of mold of yeast to a feed. CONSTITUTION:A large amount of a mold of a yeast such as baker's yeast, wine yeast, Sake yeast, beer yeast, etc. is cultivated, a self digestion or digestion promoter is added to it, and the cell membrane of a mold of yeast is disrupted, to give a digestion extract which is treated in such a way that effective components in the cell are digested and absorbed in organisms of fishes and proper tasty components are increased, and the extract is added to a feed for fish farming. Both palatability and growth are improved by using the feed.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a fish feed containing a digested extract of yeast cells.

What is generally required of fish feed is that it is highly palatable to fish, has a large feed intake, and has high feed efficiency. Currently used aquaculture feeds include fresh fish, frozen fish (including shrimp, shrimp, and sea bream meat), and oysters that belong to live feed, and paste feed that is mixed with water and mixed with powder that belongs to compound feed. Alternatively, there are Oregon pellets, which are extruded and mixed with live bait, and crumple pellets, porous pellets, and rod-shaped pellets that are molded into a solid form and dried and fed as is or with the addition of an appropriate amount of water or oil. Furthermore, there are additive feeds containing vitamins and other various substances.

The present invention has been completed by discovering that the feeding and growth of fish and crustaceans can be significantly promoted by adding a digested extract of yeast cells described below to these feeds.

The yeast cells referred to in the present invention include, for example, bakery yeast, wine yeast, sake yeast, beer yeast, etc., and the digested extract refers to yeast cells that have been cultured in large quantities by autolysis or by adding a digestion promoter. This destroys the cell membrane of yeast cells, making it easier for fish to digest and absorb the active ingredients inside the cells.
Furthermore, it refers to products that have been treated to increase appropriate flavor components.

Furthermore, the above-mentioned digestive extract as used in the present invention may be desired to be in a liquid state or to be dried and powdered (- whichever is acceptable).
.

In carrying out the present invention, a liquid digested extract of yeast cells or a dry powder thereof (hereinafter referred to as yeast extract powder) is used, and usually 0.01 to 5.
．． It is preferable that it is contained in an amount of 0%. This content is based on fish species,
It goes without saying that the choice should be made appropriately depending on the developmental stage, feed composition, etc.

As a result of numerous tests conducted until the present invention was completed, it was discovered that when unaki were fed with and without a digested extract of yeast cells, the unaki first flocked to the former to feed.

In addition, although fish, especially farmed fish, have a habit of gathering on familiar feed that is fed daily, the former, that is, on feed that has been deprived of digested extracts of yeast cells, attracts the yeast cells. The word string effect of the digested extract was fully demonstrated.

Furthermore, perhaps because the digested extract of yeast cells contains proteolytic intermediates M (pebquid, etc.), a remarkable growth effect was observed when added to feed for young fish.

Next, aspects of the present invention will be illustrated by test examples and examples.

Test Example] 50% yeast milk of commercially available baker's yeast (Saccharomyces cerevisiae) cultured using blackstrap molasses was mixed with 40% to 40% yeast milk.
Autolysis was carried out by stirring for 25 hours while heating to 5°C.

Next, this was centrifuged, the supernatant liquid was collected, and after being appropriately concentrated, it was applied to a spray dryer to obtain a yeast extract powder. The composition of the yeast extract powder thus obtained was as follows.

1-Shell component (part) Total nitrogen 1004 Direct 7.30 Amino ♀ element [F. Water splitting - Carbohydrate 1128 Nucleic acid [RNA 7°34D
NA 0.80 Crude Ash Content 8.72 Water
Minutes 6932,
Amino acid composition (%) 3° (direct) (hydrolysis) G 1 y2.06 3.20Aha
4.33 4.98Vat
3.49 3.92 Leu
3,34 3.711
1eu 2.72 3.0
8Asp 3.22 6.91G
1 u 5.48 10.42Se
r 3.28 3.32
Thr 3.58 3.
23Tyr O,220,28 Phe 2.25 2.56'Pr
o 1.71 2.44
Arg 2.59 3.
20Lys 4,63 6.00C
ys troce 1.60Me
t O,810,67 H4s 1.27 1.
54Try O, 640, 62 Total 45.62 61.68 (7
,30%N) (9,87%NNH30,190,
72 Vitamin (μyiy yeast extract) B 1 17.25 B2 167.50 B6 31.25 Ca-pant 95.00Biot
O, 48 Ni a 580 Inosit 1400 Inorganic (mg/100p yeast extract) Fe
9.28K
360O Na 40 Mg 200 Ca 60 Mn 1.41Cu
1.15 P 1530 Zn 5.55 Example 1 As shown in Table 1 below, the yeast extract powder obtained in Test Example 1 was added to the initial feed thick powder for sweetfish mainly consisting of fish meal.
, 0.1, 0.5, 1, and 5% of the mixed powder was granulated and dried to obtain a granular test feed.

A rearing test was conducted by feeding artificial feeds A, B, C, D, and E thus obtained to sweetfish larvae.

The test method was to house 1000 sweetfish larvae each 60 days after hatching (average total length 25.3 mm) in a 5-ounce + 7-light aquarium, and feed them 4 to 6 times/day with each test feed. The test period is 60 days.

Table 1: Composition of test feed (%) The results are shown in Table 2, and the yeast extract powder added feed group (B-E) of the present invention had good growth and survival.

Table 2 Test Results Example 2 Yeast extract powder was added to eel feed mainly consisting of fishmeal as shown in Table 3 below at 0, 0.05, 0.1, 0.5,
A mixed powder test feed to which 1 tsp was added was prepared and fed to eels, and the feeding rate was measured. The feed was mixed with water and given as a paste.

The test method was to stock approximately 20,000 eels with an average size of xsoy in a concrete aquarium of approximately 100 m, administer twice the daily appropriate amount of food, and measure the amount of food consumed within a certain period of time (10 minutes). It was measured. The water temperature was kept at 20°C.

Each of the test feeds B-E was compared with the control group A feed for 2 days.

The feeding rate index was expressed as the feeding amount of each test feed B to E when the feeding amount of feed A was set as 100.

Test Table 3 Feed composition (%) The results are shown in Table 4, and the feed added with the yeast extract powder of the present invention was found to have a v3 effect of promoting feeding rate, and was effective as eel feed. sex has improved.

Table 4 Test Results Example 3 As shown in Table 5, 0 and 0.5 concentrations of yeast extract powder were added to the feed powder for sweetfish, which was mainly composed of fishmeal.

] was added in an amount of 5%, mixed uniformly, and formed into a pellet after pellet formation.

The test feeds A, B, C, and D thus obtained were fed to sweetfish, and the time to satiation was measured.

The test method was to stock 316 sweetfish each with an average size of 9.1 f on two 2 m'' Marugame sides, and use feed A as a control group, and feed B and CID in order to compare satiety.

The satiation amount was the total amount between 41-1, and the testability was determined by replacing El every time. Feeding was carried out three times a day, and during the first two feedings, food was fed between meals (15 minutes of feeding), and this value was taken as the satiation amount.The last feeding was fed A feed, and the feeding amount was adjusted to the same amount during the first period. . Water temperature was 18-20°C.

The satiation index is: A - DtK satiation amount ÷ A-section satiation amount ×
It was set as 100.

Table 5: Mixed composition of test feed (%) The results are shown in Table 6, and an increase in satiety was observed in both feed groups B-D containing the yeast extract powder of the present invention compared to feed A. It was done.

Table 6 Test Results Example 4゜Yeast extract powder was added to the fish meal-based feed powder shown in Table 7 below at 0, 0,05, respectively.

The feeds containing 0.1 and 0.511% were mixed uniformly and formed into pellets of 4.5 diameter sea bream to obtain test feed.

Test feeds A, B, C, and D thus obtained.

A rearing test was conducted by feeding E.

A certain amount (daily feeding rate: 1.7 to 1.9%) of the above feed was fed within a certain period of time.

The test period is 40 days.

Table 7 Composition of test feed (H) The test results are as shown in Table 8, and the yeast extract powder-added feed of the present invention immediately achieved %B+ C+ in terms of growth and feed efficiency.
Those fed D + E performed well. In terms of feeding properties, the yeast extract powder-added feed was also better. .

Table 8 Test Results Example 5゜A feed containing 0.0.5% yeast extract powder was mixed uniformly with the powdered feed for red sea bream mainly consisting of fishmeal as shown in Table 9 below. , and molded into pellets with a diameter of 2.4 wn to obtain test feed.

A rearing test was conducted by feeding test feeds A and B thus obtained to red sea bream.

The test method was to release 50 red sea bream with an average body weight of 11F each, and administer the same to each test area A and B twice a day to saturation. The water tank has a capacity of 150L and the test period is 42 days.

The test results are shown in Table 9, and the yeast extract powder-added feed of the present invention had good feed intake and weight gain.

Table 9 Test Results Example 6 Yeast extract powder was added to the squid meal-based feed powder for Kuruma shrimp shown in Table 11 below at 0, 0.1 and 0.1, respectively.
The feed containing 0.5% and 1% is mixed uniformly, water is added, extrusion molded using a meatball, and then dried to a diameter of 2.
A cylindrical test feed with a length of 10 to 15 sieves was obtained.

A breeding test was conducted by feeding test feeds A, B, C, and D thus obtained to Kuramaehi.

The test method was to release 24 Kuruma shrimp each with an average body weight of 1.21 to 128 y, and feed each test feed in a satiated amount (the amount of remaining feed was subtracted from the amount fed each day).

The water tank had a capacity of 76 cm, and the water temperature was 21 to 24°C.

The test period is 28 days.

Table 11 Composition of test feed (%) The test results are as shown in Table 12, and the yeast extract powder-added feed group of the present invention had good results in terms of both feeding ability and growth.

Table 12 Test results 236

Claims (1)

[Claims] Fish feed containing digested yeast extract as an active ingredient