JPS597103A - Agricultural and horticultural germicide - Google Patents

Abstract

PURPOSE:The titled germicide, containing a kasugamycin and a substituted triazole derivative as active constituents, capable of exhibiting improved synergistic effects on the control of vegetables and fruit trees, e.g. powdery mildew of cucumbers, in a small amount of the chemicals, and having the residual effect and rainfall resistance. CONSTITUTION:An agricultural and horticultural germicide containing (A) a kasugamycin having a controlling activity against blast of rice plants, leaf mold of tomato, powdery mildew, scab and anthracnose of cucumbers, etc. as an antibiotic substance agricultural chemical having a high safety or a salt thereof and (B) a substituted triazole derivative of the formula (X is <=2 halogens or lower haloalkyl; Y is <=2 halogens) at 1:(0.1-10) weight ratio between the components (B) and (A) as active constituents. The above-mentioned mixture is useful for controlling various blights, e.g. rust, anthracnose and a blight caused by Mycosphaerella melonis of cucumbers, leaf mold of tomato, brown rust of wheat, rust of soybean, rust of pear and blast of rice plants. The selecting action of the kasugamycin on resistant germs can be prevented.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a novel fungicidal agent for Jllkl and Tl, which contains two types of fungicidal ingredients having different action characteristics. In other words, this book @ Ming uses kasugamycin or its salts (referred to as K8M) and the general formula (
The object of the present invention is to provide a MIII1 artistic fungicide which is blended with at least one novel substituted triazole derivative represented by I1.

General formula (I) and Y represents up to two halogen atoms.

The agricultural and horticultural fungicide of the present invention is characterized by expanding the practical disease control spectrum by mutually enhancing the disease control effects of each agent and by compensating for each other's shortcomings. It is possible to achieve extremely stable and high pesticidal effects just by spraying a large amount.

In particular, its control effect is effective against cucumber downy mildew, cucumber charcoal dragon disease, cucumber vine blight, wheat rot, soybean rust,
It is effective against important diseases of vegetables and fruit trees, such as apple scab and pear scab, due to an extremely pronounced synergistic effect that is difficult to imagine from spraying each agent alone.

The facilities that cultivate these arts are becoming popular throughout the country, especially in the suburbs of cities. The cultivation conditions of these greenhouse horticulture systems are very different from the general plain conditions, and due to the high humidity conditions under heavy fertilization cultivation, various types of diseases are occurring rapidly and causing significant damage. As a countermeasure against this problem, it is common practice to use non-labor-saving chemical control techniques, such as spraying various chemicals at high concentrations many times before and after the outbreak of the disease and just before harvest. Such multiple-ended chemical spraying poses a major problem from the viewpoint of crop contamination, chemical residue in crops, and health management of the parties spraying the chemical.

Conventional agricultural and horticultural fungicides include organic mercury agents such as PMA (phenylmercuric acetate) and BMP (ethylmercuric phosphate), zineb (ethylene bisdithiocarbamate lead salt) and maneb (ethylene bisdithiocarbamate manganese salt). Sudo dithiocarbamate agents, PCP (pentachlorophenol) and TPN
(Tetrachloroisophthalonitrile) and other chlorine agents whose activity against plant pathogens is relatively non-selective, that is, agents that have inhibitory effects on many points of action within IVJK, are conveniently used. It's here. However, some of these drugs pose problems regarding environmental pollution and systemic toxicity, and their use is currently prohibited and their use is restricted. In recent years, since chemicals are used in the closed environment of greenhouse horticulture as mentioned above, chemicals with the lowest possible risk of environmental pollution and systemic toxicity have been loaded into KSM,
Antibiotics such as polyoxin and streptomycin%
Organic phosphors such as IBP (0,0-diisopropyl S-benzylthiophosphate) and gDDP (0-ethyl 8,8-diphenyldithiophosphate), benomyl [
Methyl 1-(n-butylcarbamoyl)benzimidazole-2-yl-bamate], thiophanate methyl (1,2-di(8-methoxycarbonyl-2-thioureido)benzene), etc. are selective and have a very localized point of action. A type of mulberry shaving was developed, and it has been used over a wide range of areas due to its long-lasting disease control effect.
Shinam et al. have found that repeated and continuous use of these selective pesticides has resulted in a 4-degree resistance to the drugs, leading to a decline in the control effect of spraying the pesticides, which is a major problem in disease control. The reality is that this is an obstacle.

Therefore, there is a strong desire to develop a multi-effective IP drug that can replace C1 and has low toxicity and does not pollute the environment. .

The present inventors can simultaneously control various diseases with low mulberry
In order to develop a modern fungicide, we conducted intensive research by combining various fungicide with different activities. As a result, as described above, a novel and practical fungicide for agriculture and horticulture was created by combining KPM and each substituted triazole conductor represented by the general formula fIl.

KSM, one of the M active ingredients of the present invention, has been widely used as an antibiotic 5R pickled mulberry to control rice blast disease due to its safety. It has also been found to have good control activity against mildew, scabrous blight, and anthrax. However, the control effect of KSM against these diseases has a strong therapeutic character, and when it is sprayed prophylactically, it lacks residual efficacy and T11 resistance, so it has drawbacks that make it difficult to expect practical effects when used as a single agent. have

In addition, one of the active ingredients, a substituted triazole derivative represented by the general formula (II), is disclosed in Japanese Patent Application No. 56-1684
This is a compound disclosed in the specification of No. 9.

The present inventors combined 8M, which has such drawbacks, with at least one substituted triazole derivative represented by the general formula I11, and surprisingly, they were able to cure cucumber powdery mildew, cucumber scab, and cucumber charcoal. A small number of sprays of mulberry can be extremely effective against various diseases of vegetables and fruit shoots such as pox, cucumber vine blight, tomato leaf mold, wheat rot, soybean rot, and pear rot, as well as rice blast. It was found that a remarkable control effect was expressed, and the effect of preventing the appearance of 8M resistance was clearly confirmed when compared to the use of KPM alone even when applied to rice blast for several consecutive times. In other words, by simply applying the IA horticultural pesticide of the present invention to mosquitoes, it exerts a strong control effect in terms of both preventive and therapeutic effects against diseases that have a low control effect when sprayed alone. Moreover, these effects have residual effects and ampholytic properties. Furthermore, these control effects are due to the pitiful and self-evident additive effects that are completely inconceivable from the use of each drug alone, and they also block the effect of KPM in selecting resistant bacteria. The present invention has been made based on new findings that could not have been found by using the method alone.

Since the AI horticultural fungicide of the present invention has such an excellent effect, the usage value of the agent is 4 times higher than that when each agent is used alone.
A sufficient practical control effect can be obtained even if the amount is reduced to 1/10 to 1/10 or less.

Representative examples of substituted triazole derivatives that can be used in the present invention are as follows. In addition, the compound number is also referred to in the following Examples and Test Examples.

Compound 14-chlorobenzyl l''J-(2,4-dichlorophenyl) 2 (1-2゜4-triazole-
1-yl)ethanethioimidate compound 2 s,4-dichlorobenzyl N-(2゜4-dichlorophenyl)-2-(1゜2.4-triazol-1-yl) ethanethioimidate t compound 82,4 -Dichlorobenzyl N-(2゜4-dichlorophenyl)-・2-(1゜2.4-)riazole-
1-yl) Ethanethioimidate t Compound 42,4-dichlorobenzyl N-(4-chloro-
Compound 5 4-chlorobenzyl N-(2-chloro-4-
In carrying out the present invention, the active ingredient compound is diluted with a carrier and prepared in the commonly used form In4s, such as water. It may be formulated into Japanese preparations, emulsions, powders, DI, (triless) type powders, powders for flow dust, flowable preparations (sols), granules, fine granules, tablets, etc., and used according to known methods. Common carriers used in the present invention include clay, talc, bentonite, kaolin, diatomaceous earth,
Solid carriers such as silica, or benzene, xylene, toluene, kerosene, alcohols (methanol, ethanol, isopropanol, n-butanol, etc.)
Liquid carriers such as , ketones (acetone, methylethane, cyclohexane, etc.) can be used. These can be used by mixing appropriate surfactants, preservatives, etc. The mixing ratio of the active ingredient in the present invention is 1 to 10 parts of 8Mα per 1 s of IJ azole cast conductor as a mixing ratio, but depending on the application conditions and disease occurrence situation. The blending ratio can be changed as appropriate. In addition to the active ingredient of the present invention, other bactericides, insecticides, plant growth regulators, etc. may also be added.

When spraying the lk horticultural fungicide of the present invention, the amount of active ingredient is in the range of 1 to 1000 g per 10 ares, and
The degree of the active ingredient can be appropriately selected within the range of α001 to 10.

Next, examples will be shown regarding the horticultural fungicide A of the present invention, but the present invention is not limited only to the following examples.

Example 1 26 parts of a wettable powder compound, 1.2 parts of KPM hydrochloride (hereinafter KSM refers to hydrochloride), 5 parts of lauryl sulfate, 15 parts of white carbon, 8 parts of lignin sulfonic acid.
and 708 parts of finely powdered clay were uniformly mixed and pulverized to obtain a wettable powder.

Example 2 605 parts of powder compound, 1 part of K8Mα, white carbon α8
1 part and 1 part of fine powder clay 9a are uniformly mixed and pulverized to obtain a powder.

Next, m1ll of the present invention! 1. The disease control effect of the cocoonicide for entertainment will be explained using test examples.

Test Example 1 Cucumber anthrax disease control effect (pot test) Example 1 was applied to the entire surface of blue cucumber (variety: Tsukuba Shirobo) 0) 5-leaf stage seedlings in a plastic pot with a diameter of 15 cIn.
Similarly, 11111JL and a predetermined M It dilution of a hydrating powder containing the active ingredients shown in Table 1 were sprayed at a rate of 16a per pot. Next, cucumber anthrax (Coletotrichum lagemerium).

conidia of tricbIAm Iagenarium) were suspended in 50 ppmmi of Tween 20 (trade name of polyoxyethylene sorbitan monolaurate manufactured by Kao Atlas Co., Ltd.) and used as a seeding source to incubate 2-day-old cucumbers. The elephants were spray-inoculated on the front and back sides. After inoculation, the cucumber fungus was kept in a greenhouse at 24°C all day and night, and then cultivated in a controlled greenhouse at 24°C to 26°C and humidity of 80 to 96%.

Disease* Seven days after inoculation, the number of cucumber anthracnose disease lesions was investigated on the second to 14th cucumber leaves, and the control value (knowledge) was determined using the following formula.

This test was conducted using a two-pot system for each drug concentration, and the average control value (chi) was calculated. The results are shown in Table 1.

Table 1 Note 1 The control agent cabtan represents N-()lichloromethylthio)-4-cyclohexene-1°2-dicarboximide.

Note 2: The numbers in parentheses indicate the average number of cucumber anthracnose lesions in plots not sprayed with mulberry chemicals.

Test Example 2 Cucumber Powdery Mildew Control Effect (Rain Resistance and Residual Effect) A blue cucumber (variety: Sagami Hanshiro) was pruned at the 4-leaf stage in a plastic pot with a diameter of 16 tM.

Then, a diluted solution of a hydrating powder containing the active ingredients shown in Table 2 at a predetermined concentration was sprayed on the front and back surfaces of cucumber leaves. Five days after the chemical spraying, the cucumbers were treated with rain for 60 minutes at 5-day intervals using an artificial rainfall device, six times at 5-day intervals.

Powdery mildew disease on cucumbers after rain treatment was mainly due to natural infection, but to accelerate the onset of the disease, the infected cucumbers were planted in plastic pots with a diameter of 5 rust (the leaves of the cucumber fungi were cored, Only cotyledons develop) on test cucumbers 4
They were arranged randomly, one pot per pot. In addition,
Cucumber oval bot for use as a seed source is % 10 2 days after spraying the chemical.
Replaced after 1 day and after 2020 days.

The onset of disease aJ4 meals are 10 days, 20 days and 8 days after drug spraying.
After 0 days, calculate the cucumber powdery mildew lesion area percentage from the 1st leaf to the 4th leaf, and calculate the control value (%) using the following formula:
was calculated.

×100 Non-tests were performed using a two-bot system for each drug concentration, and the average control value (chi) was calculated. The results are shown in Table 2.

Wiping test example 8 K8M-resistant blast fungus development inhibition test (bacteria growth box test) 1) Rice blast fungus (Pyricularia oryzae: Pyricularia 0ry) was applied to rice at the two-leaf stage.
zae) spore MfA solution was spray inoculated, and when blast lesions appeared 6 days later, a hydrating powder containing the active ingredients shown in Table 8 was sprayed, and the same drug was further sprayed 6 days later. . Twenty days after inoculation with the diseased fungus, the diseased leaves were cut out, and this was used as an inoculum source to subculture the inoculum of the rice blast fungus again by drawing 4 pieces of rice at the two-leaf stage. Thereafter, sub-inoculation every 20 days after inoculation with disease e# and chemical spraying 6 and 11 days after inoculation were repeated.

2) Cumulative number of chemical sprays: 8 times, 12 times, 16 times and 20 times
9 days after each drug application, 20 lesions were detected in each drug area.
A strain of rice blast fungus was isolated and cultured in a PEA medium in a test tube at 24°C for 10 days.

8) Cut out the bacterium-containing special layer from P8A medium and attach it to the mould.
The microorganisms were cultured at 24° C. for 48 hours, and the minimum growth inhibition (MIC) of each isolated strain of KSM was determined. Bacteria with a MIC of 100 ppm or more against KSM's rice blast disease-prone isolates were defined as KSM-resistant bacteria.

Table 8 shows the relationship between the number of times the chemical was sprayed and the frequency of appearance of KSM-resistant blast fungi.

Table 8 Show rate.

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Claims (1)

[Claims] Kas is mycin or its salts and a substituted triazole derivative represented by the general formula (wherein X represents up to two identical or different halogen atoms or a lower haloalkyl group, and Y represents two halogen atoms) An agricultural and horticultural fungicide characterized by containing a mixture of and as an active ingredient.