JPS5934103B2 - How to activate and preserve the life of crustaceans - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for activating and preserving live Kurumae shrimp and similar crustaceans, and its purpose is to increase the vitality of live Kurumae shrimp and similar crustaceans, and to By dramatically increasing compatibility with the natural environment, aquaculture, transport,
The object of the present invention is to provide an economical method for extending survival time and increasing survival rate in farming and the like.

In general, crustaceans, especially Kuruma shrimp, have a low vitality, and during their cultivation, they are exposed to various environmental conditions that differ from their original survival conditions, such as deterioration of water quality, and they may die in large numbers due to fish diseases. There are many.

In addition, during the process of capturing, shipping, transporting, and preserving both farmed and wild animals, they are subjected to various handling conditions that differ from their original survival conditions, leading to exhaustion and some death.

Conventionally, various measures have been taken to prevent mortality during aquaculture, including proactively administering nutrients such as vitamins, and more passively, preventing and maintaining water quality.

Antibiotics have also been administered to treat fish diseases.

In addition, when handling live aquatic products outside of the normal growing environment, there are methods such as sedating them with anesthesia, making the handling environment rich in oxygen, etc., to simulate the growing environment, and minimizing fatigue. Proposed.

However, once exhausted crustaceans such as Kuruma shrimp,
None of these methods are sufficiently effective, and it is currently difficult to resuscitate and revitalize exhausted crustaceans such as Kuruma shrimp and maintain them for a longer period of time.

The present inventors have worked diligently to dramatically increase the vitality of live Kurumae shrimp and similar crustaceans and their compatibility with the external environment, and to extend the survival time and increase the survival rate during aquaculture, transportation, farming, etc. I would like to arrive at the present invention as a result of my research.

In other words, when an amino acid derivative hormone was added to seawater or artificial seawater, which is the growth environment for Kuruma shrimp and similar crustaceans, the animals surprisingly died even after being left in an aquarium for a long period of 3 to 4 weeks. The survival time was significantly prolonged and the survival rate was improved.

In particular, the effect of adding these chemicals to Kuruma shrimp and spiny shrimp is remarkable.

Kuruma shrimp (about 3
0g/fish) 50 fish were mixed with seawater to which adrenaline, an amino acid derivative hormone, was added (adrenaline 710T/u).
? /V) 301 for 24 hours.

After that, we took them out and compared them with 50 untreated Kuruma shrimp that had been similarly transported with sawdust and exhausted them, and observed their survival rate in the aquarium. After 10 days, the survival rate of the treated shrimp was 100, whereas there was no - 13 treated shrimp died, with a survival rate of 74.

After 20 days, the survival rates were 96 and 50, respectively.

This indicates that adrenaline, which is an amino acid derivative hormone, has a certain resuscitative and revitalizing effect on exhausted Kuruma shrimp.

Similar experiments were conducted with various amino acid derivative hormones, and it was confirmed that they have the same effect as epinephrine in increasing survival time and survival rate.

Amino acid derivative hormones refer to amino acid hormones such as iodoamino acids as defined by H. Heller, and amine hormones such as catecholamines and indole derivative amines.

(H, Heller: Mo1ecularaspe
cts in Comparative en-do
rinology, Gen, Comp, Endoct,
22, 315-332 (1974)) As an amino acid hormone, thyronine 3°5.3'
, mono-, di-, and tri-iodine substitution products at the 5'-position, the thyroid hormone thyroxine, and metal salts thereof, etc., gave particularly favorable results.

Among the amine hormones, catecholamines include
Dopa, dopamine, noradrenaline, epinephrine, and their hydrochlorides, tartrates, etc., and among indole derivative amines, melatonin, a pineal gland hormone, etc. gave particularly favorable results.

Examples of crustaceans to which the present invention can be applied include prawns,
There are shrimp such as white shrimp, red shrimp, white shrimp, Japanese rock shrimp, white shrimp, white shrimp, and rock shrimp lobster, and crabs such as red king crab, Japanese king crab, crab, hair crab, and Japanese rock shrimp, but this hormone is especially used for black shrimp and rock shrimp. The effect is remarkable.

Such an effect of increasing survival time and survival rate is universally observed in amino acid derivative hormones, and is probably due to the promotion of humoral coordination of hormones, that is, the promotion of metabolic regulation (especially metabolic effects in the kidneys, liver, etc.). It is thought that suppression dramatically increases the external environmental suitability of live Kurumae shrimp and similar crustaceans, resulting in increased vitality, that is, improved survival rate under a certain environment.

Hormones can be administered in any way as long as they are taken into the body fluids of live shrimp and similar crustaceans, such as a medicated bath method in which they are added and dissolved in environmental water, or suspended in environmental water. Any method may be used, such as feeding, mixing with feed, or injecting.

To exemplify the simplest medicinal bath method, for example, in a 20°C water tank, the effect is sufficiently exerted by acting at a concentration of o, i to 100 ppm, more preferably 0.5 to 20 ppIll, for 16 to 48 hours. .

The present invention may be used alone or in combination with two or more of the hormones, and its effectiveness is further demonstrated when used in combination with known nutritional supplements such as vitamins, nutritional salts, and antibiotics. It is something to do.

As described above, the present invention improves the environmental adaptability, that is, the vitality, of living Kuruma shrimp and similar crustaceans through the metabolic regulation effect in the body of living organisms, and improves the ability to adapt to the environment, that is, the vitality of living shrimp and similar crustaceans.
It can be used for the purpose of increasing its vitality, survival time, and survival rate regardless of its cultivation.

It is also an economically advantageous method because sufficient efficacy can be expressed at extremely low concentrations and in a short period of time.

The effects of the present invention will be illustrated below with reference to Examples.

Example 1 The results of administering adrenaline, which is an amino acid hormone, at different times are shown in comparison with those without administration.

Put 301 natural seawater into the aquarium and add 1yn of adrenaline.
The mixture was added to a concentration of 9/12, and 50 Kuruma shrimp transported through sawdust were housed.

The temperature of the seawater was controlled to 20°C ± 2°C with aeration, and after leaving it for a certain period of time, it was placed in an aquarium in which water quality such as water temperature, pH1NH3 concentration, COD, and humidity was maintained, and survival time was measured.

The residence time of the Kuruma shrimp used in the sawdust was 4 hours, and the shrimp had a uniform size of 30 g/fish on average.

The results are shown in Table-1. *The survival rate of Kuruma shrimp without the addition of 1m9/l of adrenaline decreased to 90% and 6c10 after 21 days, whereas the survival rate of shrimp treated with adrenaline for 24 hours was 100% and 90%, respectively. , showing remarkable effects.

From the above, when administered under certain treatment conditions, the activation and retention effects of adrenaline are significant.

Example 2 The results of administering adrenaline, an amino acid hormone, at varying concentrations are shown in comparison with the case of no administration.

The procedure was the same as in Example 1 except that the additive concentration was 1 to 10 m.9/11 treatment time was changed to 16 hours.

The results are shown in Table-2.

*The survival rate of Kuruma shrimp treated with adrenaline without administration for 16 hours decreased to 7 out of 90 and 21 days to 4.
Those added were 100 parts and 96 parts, respectively, showing remarkable effects, and additions of 5 to 411 parts/l also showed effects.

From the above, when administered under certain treatment conditions, the activation and retention effects of adrenaline are significant.

Example 3 The results of administering various amino acid derivative hormones are shown as examples, with no administration as a comparative example.

The process was the same as in Example 1 except that the additive concentration was 1-10-/11 and the treatment time was 24 hours.

The results are shown in Table-3.

The survival rate for those without administration was 62% on day 14 and 62% on day 21.
In contrast, adrenaline, thyroxine, and melatonin levels were 18 to 38 on the 14th day, and 24 on the 21st day.
The survival rate was ~50 times higher, and epinephrine was particularly effective.

From the above, it is clear that administration of amino acid hormones has activation and maintenance effects.

The procedure was the same as in Example 1, except that the Japanese prawns were changed to spiny lobsters, the number of housed lobsters was changed to 50, and the amount added was 5 yntp/l.

The average weight of spiny lobsters is 18,097 fish, and they are all uniform in size.

The results are shown in Table-4.

The survival rate of untreated spiny lobsters decreased to 80% for the 7th caliber and well over 0 for the 21st caliber, whereas those treated for 48 hours showed high survival rates of 100% and 90f0, respectively.

From the above, the activation and retention effects of amino acid derivative hormones are remarkable.

Claims (1)

[Claims] 1. A method for activating and maintaining crustaceans, which comprises administering an amino acid derivative hormone.