KR100811110B1 - Feed additive containing alpha-lipoic acid capsule composition stabilized in water phase for increasing immunity of fish and method for using the same - Google Patents

Abstract

A method of using a food additive composition for increasing the immunity of fish containing a water-dispersible alpha lipoic acid capsule composition is provided to ensure an excellent preventing effect on the occurrence of iridovirus or a disease whose cause is not known. A feed additive composition for increasing immunity of fish contains a water-dispersible alpha lipoic acid capsule composition which is obtained by mixing 0.5 to 20.0% by weight of alpha-lipoic acid, 0.5 to 25.0% by weight of one or more water-insoluble polymers having self-emulsifying properties, 0.1 to 12.0% by weight of one or more amino acids; and a balance of water, a hydrophilic solvent or a mixture thereof and encapsulating. The fish is carp, catfish, loach, Oreochromis niloticus, parrot fish(Oplegnathus fasciatus), eel, Sebastes schlegeli, Konosirus punctatus and the like. The amount of the water-dispersible alpha lipoic acid capsule composition which is added to feed is 150 to 2,000ppm per 1 ton feed. A daily dose of alpha lipoic acid comprising the water-dispersible alpha lipoic acid capsule composition is 759 to 10,000mg based on a fish cage.

Description

Feed additive containing alpha-lipoic acid capsule composition stabilized in water phase for increasing immunity of fish and Method for using the same}

The present invention relates to a feed additive for boosting immunity of fish containing a water dispersible α-lipoic acid capsule composition and a method for enhancing immunity of fish using the feed additive, and more specifically, α-lipoic acid, self-emulsifying properties. Water-insoluble polymer having a water or a mixture thereof, amino acids and water, a hydrophilic solvent or a mixture thereof as a mixture; the immunity enhancing feed additive and the feed additive of the fish containing the water-dispersible α- lipoic acid capsule composition encapsulated by mixing It relates to a method for enhancing the immunity of fish, characterized in that the added feed to the fish.

α-lipoic acid has various names such as thioctic acid, 6,8-dithiooctanoic acid, or 1,2-diethylene-3 pentanoic acid. In 1951, L. J Reed et al. The traces were crystallized from the liver and named alpha-lipoic acid, followed by synthesis.

α-lipoic acid is produced as a low concentration of natural products in the form of R enantiomers in plant and animal cells. Physiological action of hydrophilic and lipophilic media of α-lipoic acid, originally found as a growth factor, is as a coenzyme and as an antioxidant in the oxidative decarboxylation of α-keto carboxylic acids, for example pyruvate. α-lipoic acid plays a role in the regeneration of vitamin C, vitamin E, glutathione and coenzyme Q10, and when used as animal feed, prevents anti-aging (PCT International Application No. PCT / US01 / 02713) and natural killer cell activity in animals. It is effective in increasing immune function (PCT International Application No. PCT / US05 / 42805).

In addition, when applied to the skin is known to have the effect of removing the active oxygen and to prevent wrinkles, when applied to not only cosmetic compositions but also pharmaceutical compositions, household goods and the like has been expected to exhibit an excellent function.

However, α-lipoic acid has difficulty in formulating due to its very low solubility and dispersibility in water, and has a problem of discoloration and discoloration due to intrinsic odor by the sulfur component and separation of the bond to thiol in sunlight. For this reason, despite its excellent performance, it is very limited to use a sufficient amount of α-lipoic acid as an active ingredient in medicine, food, cosmetics, and the like. Particularly, when α-lipoic acid is used as an active substance for fish, α-lipoic acid crystals have low solubility and dispersibility in water, and thus, α-lipoic acid crystals are generally used in an amount of about 100 ppm per ton of feed. There is a limit to evenly administering itself to a large number of fish in a certain area. In addition, due to the low stability of α-lipoic acid, there is a problem that activity decreases over time.

On the other hand, with the recent development of artificial seedling production technology of fish and the rapid development of marine fish farming industry, high-quality fish such as flounder, skinfish rock, sea bream, perch and puffer fish have been spotlighted as the main farming species. Due to the rapid growth of aquaculture, the surrounding environment, including coastal seawater in Korea, is likely to be contaminated by Herpervirus, Birnavirus, and Iridovirus, the major pathogenic viruses of fish diseases. There is always a risk of disease caused by viruses, and fish farming is severely damaged by the infection of these pathogens.

In particular, tens of millions of domes are killed every year due to the viral disease caused by Iridovirus infection.In addition, once such a viral disease occurs, treatment is very difficult and economic loss is large. It has a huge economic impact on fish farming and adversely affects fish stocks. Iridovirus, which infects kidneys, spleen, nerve cells, or fish immune-related cells such as domes, perch, flounder, and defenses, causes massive mortality of these infected fish, which did not occur in Korea until 1997, Mass mortality of rock domes on caged farms in the South Coast began to occur (Sohn et al. J. Fish Path, 13 (2): 121-127, 2000), which has caused a great deal of economic damage to fishermen to date.

Accordingly, the present inventors have found that the water-dispersible α-lipoic acid (PCT International Application No. PCT / KR2006 / 001574) capsule composition invented by the present inventors is dispersed in α-lipoic acid, a water-insoluble polymer having self-emulsifying properties, and a hydrophilic solvent. By encapsulating α-lipoic acid by mixing, it has excellent stability and high dispersibility in water. When the α-lipoic acid capsule composition is used as a feed additive for fish, the water-dispersible α-lipoic acid is diluted in water. It can be evenly administered to a large number of fish in a certain area by the method of evenly applied to the feed, and as a result of administering the feed additive to the fish, the α- lipoic acid capsule composition is the immune enhancing effect of the fish and preventive effect against various viruses It was confirmed that there is, and completed the present invention.

It is an object of the present invention to contain a water-dispersible α-lipoic acid capsule composition encapsulated by mixing α-lipoic acid, a water-insoluble polymer having self-emulsifying properties or a mixture thereof, an amino acid and a residual amount of water, a hydrophilic solvent or a mixture thereof; It is to provide a method for enhancing the immunity of the fish, characterized in that the feed additive for immunity enhancement of the fish and the feed to which the feed additive is added to the fish.

In general, α-lipoic acid has a very low solubility and dispersibility in water, so it is very difficult to evenly supply fish living in a certain area of water, and there is a problem in that activity decreases over time. The α-lipoic acid capsule composition used as a feed additive in the present invention is mixed with water-dispersed α-lipoic acid, a water-insoluble polymer having self-emulsifying properties, and a hydrophilic solvent to encapsulate α-lipoic acid, thereby providing excellent stability and dispersibility in water. There is a very high characteristic.

The present invention relates to a feed additive for enhancing immunity of fish containing a water dispersible α-lipoic acid capsule composition and a method for enhancing immunity of fish using the feed additive.

The present invention relates to a composition of a fish containing a water-dispersible α-lipoic acid capsule composition encapsulated by mixing α-lipoic acid, a water-insoluble polymer having self-emulsifying properties or a mixture thereof, amino acids and water as a residual amount, a hydrophilic solvent or a mixture thereof. It relates to a feed additive for enhancing immunity.

The water-dispersible α-lipoic acid capsule composition used in the present invention is characterized in that it comprises water, a hydrophilic solvent or a mixture thereof as α-lipoic acid, a water-insoluble polymer having a self-emulsifying property or a mixture thereof, amino acids and the balance thereof. . More specifically, the water-dispersible α-lipoic acid capsule composition is 0.5-20.0 wt% of α-lipoic acid, 0.5-25.0 wt% of one or more water-insoluble polymers having self-emulsifying properties, 0.1-12.0 wt% and one or more amino acids It is characterized in that it comprises water, a hydrophilic solvent or a mixture thereof. The α-lipoic acid is preferably contained in an amount of 0.5 to 20.0% by weight based on the total weight of the α-lipoic acid capsule composition. If the content is less than 0.5% by weight, the amount of α-lipoic acid for which α-lipoic acid can be stabilized is too small, and if it exceeds 20.0% by weight, unencapsulated α-lipoic acid remains in excess water phase, making it difficult to stabilize. have.

The method for preparing the water dispersible α-lipoic acid capsule composition may include a first step of mixing a hydrophilic solvent, α-lipoic acid, a water-insoluble polymer having self-emulsifying properties, or a mixture thereof at room temperature; A second step of adding amino acids to the mixture of the first step; And a third step of preparing a capsule by adding water, a hydrophilic solvent or a mixture thereof to the mixture of the second step.

Another method of preparing the water-dispersible α-lipoic acid capsule composition may include a first step of mixing a hydrophilic solvent, α-lipoic acid, a water-insoluble polymer having an emulsifying property, an amphiphilic polymer, or a mixture thereof at room temperature; A second step of adding water, a hydrophilic solvent or a mixture thereof to the mixture of the first step to dissolve it; And a third step of mixing amino acids into the mixture of the second step.

The water dispersible α-lipoic acid capsule composition is a mixture of water-dispersed α-lipoic acid, a water-insoluble polymer having self-emulsifying properties, and a hydrophilic solvent to encapsulate α-lipoic acid. Not only can it be effectively inhibited from being degraded by, but also can maintain the stability of α-lipoic acid from interaction with other substances added together when applied to cosmetics, foods and pharmaceuticals.

In particular, the stabilizing capsule of alpha lipoic acid prepared according to the method of encapsulating alpha lipoic acid in a water-insoluble polymer having self-emulsifying properties and dispersing it in an aqueous phase containing amino acids has a water-insoluble property in which alpha lipoic acid has a self-emulsifying property in a solid phase. It is encapsulated by the polymer and can be stably maintained from external stimuli such as oxygen, water, and light, and when some alpha lipoic acid contained in the solid phase is eluted in water, it can be stabilized in the water phase.

In the present invention, when the α-lipoic acid capsule composition is used as a feed additive for immunity enhancement of fish, α-lipoic acid is preferably contained in an amount of 0.5 to 20.0% by weight based on the total weight of the α-lipoic acid capsule composition, and 0.5 to More preferably, it is contained in an amount of 5.0% by weight. If the α-lipoic acid content of the α-lipoic acid capsule composition exceeds 5.0% by weight, there is a problem in that the amount of water required to dilute the α-lipoic acid capsule composition during the addition to the feed is too large.

In the present invention, the fish is not particularly limited, but freshwater species including carp, catfish, loach, or sea bream (tilapia), or sea bream, sheep manure (eel), rockfish (zombola), trout, mullet, sea bass, red snapper, black sea bream It is preferable that it is a saltwater fish species including a crab, a prawn, a shrimp, a saltfish, a goldfish, a sea squid, or a crab.

In the present invention, the immunity of the fish is preferably the immunity of the fish against the virus, and more preferably the immunity of the fish against the virus.

Another aspect of the present invention comprises the steps of adding a feed additive containing a water dispersible α- lipoic acid capsule composition to the feed and administering a mixture of the feed additive and the feed to the fish to improve the immunity of the fish It relates to a method of enhancing.

The amount of the water-dispersible α-lipoic acid capsule composition added to the feed in the present invention is not particularly limited, but it is preferable to add the water-dispersible α-lipoic acid capsule composition in an amount of 150-2000 mL relative to 1 ton of feed. The amount of water-dispersible α-lipoic acid capsule composition added to the feed is in the range of 150 to 2000 ppm in terms of ppm based on the feed (the density of the water-dispersible α-lipoic acid capsule composition is close to 1 g / mL).

In general, considering the amount of feed administered to fish, if the amount of water-dispersible α-lipoic acid capsule composition is less than 150 ppm, the amount of α-lipoic acid capsule composition administered per fish is too low, and if it exceeds 2000 ppm, it is administered per fish. The amount of α-lipoic acid capsule composition to be made is too high.

In the present invention, when the feed additive containing the water-dispersible α-lipoic acid capsule composition is added to fish, the dosage of the α-lipoic acid capsule composition is not particularly limited, but one cage (one cage is fish 10,000) It is preferable that it is 50-20,000 mg / day, and it is more preferable that it is 750-10,000 mg / day with respect to one cage. When the dose of the α-lipoic acid capsule composition is 750 to 10,0000 mg / day for one cage, the immunity and virus prevention effect of the fish by the α-lipoic acid capsule composition is excellent.

In the present invention, a method of adding a feed additive containing the α-lipoic acid capsule composition to a feed is characterized in that the α-lipoic acid capsule composition is diluted in water and then uniformly applied to the feed using a sprayer or a mixer (loop). It is done.

In general, α-lipoic acid has a very low solubility and dispersibility in water, making it difficult to uniformly supply fish in a certain area of water. The α-lipoic acid capsule composition used as a feed additive in the present invention is mixed with water-dispersed α-lipoic acid, a water-insoluble polymer having self-emulsifying properties, and a hydrophilic solvent to encapsulate α-lipoic acid, thereby having excellent stability and dispersibility in water. There is a high characteristic. By diluting the α-lipoic acid capsule composition in water and then uniformly applying the feed to the feed by using a nebulizer or a mixer (a loop), the feed is supplied to the fish that lives in the water, and the α-lipoic acid is supplied to the fish. Can be supplied evenly.

Hereinafter, the content of the present invention will be described in more detail with reference to Examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

Example: Field Test Using Water Dispersible α-Lipoic Acid Capsule Composition

Example 1

10 ml of the water dispersible α-lipoic acid capsule composition containing 1.5 wt% α-lipoic acid was diluted in 100 ml of water and sprayed onto 20 kg of feed using a sprayer so that the composition was evenly applied to the feed. The 60,000 rice bream was fed three times a day, and one of three times a day was fed a feed containing the composition (test group). It jumped to 60,000 stone domes.

In the case of the control group, the feed without the composition was supplied three times a day. Here, per cage (one cage means 10,000 fish), the feed amount was 5 kg per feed. The administration period was 15 days from September 7 to September 21.

Example 2

40 ml of the water-dispersible α-lipoic acid capsule composition containing 1.5 wt% α-lipoic acid was diluted in 100 ml of water and sprayed onto 20 kg of feed using a sprayer so that the composition was evenly applied to the feed. The rest of the conditions were the same as in Example 1.

Example 3

3 ml of the water dispersible α-lipoic acid capsule composition containing 5.0 wt% α-lipoic acid was diluted in 100 ml of water and sprayed onto 20 kg of feed using a sprayer so that the composition was evenly applied to the feed. The rest of the conditions were the same as in Example 1.

Example 4

12 ml of the water-dispersible α-lipoic acid capsule composition containing 5.0 wt% α-lipoic acid was diluted in 100 ml of water and sprayed onto 20 kg of feed using a sprayer so that the composition was evenly applied to the feed. The rest of the conditions were the same as in Example 1.

Table 1 shows the results of Examples 1 to 4 above. According to the experimental results, the final mortality of the test groups of Examples 1 to 4 was found to be 20-25%, while the final mortality of the control group was 50-60%. In addition, in the case of fish body weight, the test groups of Examples 1 to 4 were 115 to 140 g / horse, whereas the control group was 100 to 110 g / horse.

Since the administration period in the experiment is a period of Irivirus virus on the sea bream has already been identified in the cage, the mortality due to the experimental results can be regarded mainly as the mortality caused by Irido virus. The low mortality in the test group compared to the control group indicates that the immunity of sea fish, sea bream, was significantly enhanced by the water dispersible α-lipoic acid capsule composition, and in particular, the immunity against Irido virus was markedly increased.

In addition, when the maridang body weight of the test group was larger than the control group, the water-dispersible α-lipoic acid capsule composition not only enhances immunity of fish but also has an effect of enhancing appetite, and thus is useful as a feed additive for fish. Judging.

Example 1 Example 2 Example 3 Example 4 Control  Weight Percent of α-Lipoic Acid in α-Lipoic Acid Capsule Composition   1.5   1.5   5.0   5.0   0  Amount of α-lipoic acid capsule composition added per ton of feed (mL)   500   2,000   150   600   0  Dose of α-lipoic acid capsule composition per cage (mg / day)   2,500   10,000   750   3,000   0  Final mortality (%)  20  22  21  25  50-60  Body weight (g / mari)  130-140  115-135  117-141  125-140  100-110

Example 5

Only 100,000 feeds were fed from August 23 and the rest of the conditions were the same as in Example 1. One day after feeding, unexplained mortality began. Mass mortality caused by hot water and streptococci occurred in the side cages, suggesting mixed infection.

On August 25, two days after the mortality occurred, the feed containing the composition was supplied by the feeding method, and two days after the feeding, mortality no longer occurred. As such, the administration of the water dispersible α-lipoic acid capsule composition may be effective in enhancing the antioxidant and immunity of fish in a situation where the cause is uncertain or a complex infection is concerned.

Example 6

630,000 woofers were fed from September 12 to September 15, with the rest of the conditions being the same as in Example 1. After 10 days, the feed intake of the test group increased by 30-50% compared to the control intake of the control group (from 15 kg of 15 kg to 20 to 20 of live feed). Since the increase in feed intake means weight gain and growth promotion, it was determined that the administration of the water dispersible α-lipoic acid capsule composition acted on the fish.

Example 7

The experiment was performed on 100 freshwater fish carp, and the rest of the conditions were the same as in Example 1. As a result of visual inspection, the skin of the carp was not found at all in the control group, although the skin damage caused by the aberration and the collision with the tone was observed in the control group.

In view of the above results, it was determined that the water dispersible α-lipoic acid capsule composition has an antioxidant function and a vitamin-E strengthening effect in skin cells of fish. In addition, the experimental results of Example 7 showed that the composition can be applied to freshwater fish as well as sea fish species.

The present invention provides a method for administering an immunity-enhancing feed additive and a feed to which a feed additive is added to a fish containing a water-dispersible α-lipoic acid capsule composition encapsulated by mixing a water-insoluble polymer having a self-emulsifying property and a hydrophilic solvent. It provides a method for enhancing the immunity of fish characterized in that.

Since the α-lipoic acid capsule composition of the present invention has a very high water dispersibility, when used as a feed additive for fish, it may be evenly administered to a large number of fish in a certain area.

Α-Lipoic acid capsule composition of the present invention is very effective in enhancing the immunity of fish because of its excellent stability in water, and in particular, it is very excellent in preventing the virus and the cause of the unknown disease caused by many economic damage by the virus It can be of great help to the fishermen who are wearing them.

Claims (10)

0.5-20.0 wt% of α-lipoic acid, 0.5-25.0 wt% of one or more water-insoluble polymers having self-emulsifying properties, 0.1-12.0 wt% of one or more amino acids, and a balance of water, a hydrophilic solvent, or a mixture thereof; Immunity enhancer feed additive for fish containing the water-dispersible α-lipoic acid capsule composition encapsulated by. The method of claim 1, Immunity of the fish is a feed additive for immunity enhancement of fish, characterized in that the immunity against the virus of the fish. The method of claim 2, The virus is a feed additive for immunity enhancement of fish, characterized in that the Iri virus. The method according to any one of claims 1 to 3, Immunity enhancing feed additives for fish, characterized in that the α-lipoic acid content of the water-dispersible α-lipoic acid capsule composition is 0.5 to 5.0% by weight. The method according to any one of claims 1 to 3, The fish is a freshwater fish species, including carp, catfish, loach, and reverse sea bream (tilapia), the feed additive for immunity enhancement of fish. The method according to any one of claims 1 to 3, The fish is an immunity enhancer of fish, characterized in that the sea bream, including rock bream, sheep (eel), uruk (jeolbolak), eel, mullet, sea bass, red snapper, black sea bream, bokbok, shrimp, minnow, sagefish, tuna, and crab Feed additives for. The method of claim 1, wherein the feed additive containing the water dispersible α-lipoic acid capsule composition of any one of claims 1 to 3 is added to the feed, and the feed additive and a mixture of the feed is characterized in that the step of administering to the fish. How to enhance the immunity of the fish. The method of claim 7, wherein The amount of the water-dispersible α-lipoic acid capsule composition added to the feed is 150 ~ 2000ppm based on 1 ton of feed, characterized in that for enhancing the immunity of fish. The method of claim 7, wherein In administering the feed to which the feed additive containing the water dispersible α-lipoic acid capsule composition is added to fish, the dosage of the α-lipoic acid constituting the water dispersible α-lipoic acid capsule composition is about 750 to 10,000 mg / day. Method for enhancing the immunity of fish, characterized in that. The method of claim 7, wherein the method of adding a feed additive containing the α-lipoic acid capsule composition to the feed is characterized in that the α-lipoic acid capsule composition is diluted in water and then uniformly applied to the feed using a sprayer or a mixer. How to enhance the immunity of the fish.