JPS5928496A - 生体試料の分析方法 - Google Patents
生体試料の分析方法Info
- Publication number
- JPS5928496A JPS5928496A JP13693982A JP13693982A JPS5928496A JP S5928496 A JPS5928496 A JP S5928496A JP 13693982 A JP13693982 A JP 13693982A JP 13693982 A JP13693982 A JP 13693982A JP S5928496 A JPS5928496 A JP S5928496A
- Authority
- JP
- Japan
- Prior art keywords
- peak
- liver
- liquid chromatography
- peaks
- acetonitrile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000019423 liver disease Diseases 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims description 34
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000004811 liquid chromatography Methods 0.000 claims abstract description 16
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000001099 ammonium carbonate Substances 0.000 claims abstract description 15
- 235000012501 ammonium carbonate Nutrition 0.000 claims abstract description 15
- 239000012472 biological sample Substances 0.000 claims abstract description 9
- 230000007170 pathology Effects 0.000 claims description 5
- 238000002405 diagnostic procedure Methods 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract description 6
- 238000012856 packing Methods 0.000 abstract description 4
- 229910000077 silane Inorganic materials 0.000 abstract description 3
- 239000000377 silicon dioxide Substances 0.000 abstract description 3
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 abstract description 2
- 230000001575 pathological effect Effects 0.000 description 19
- 210000002966 serum Anatomy 0.000 description 19
- 238000004458 analytical method Methods 0.000 description 15
- 239000000523 sample Substances 0.000 description 11
- 230000002159 abnormal effect Effects 0.000 description 10
- 208000006454 hepatitis Diseases 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 210000004185 liver Anatomy 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 208000019425 cirrhosis of liver Diseases 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000000945 filler Substances 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 5
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 5
- 206010008909 Chronic Hepatitis Diseases 0.000 description 5
- 238000012742 biochemical analysis Methods 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 5
- 231100000354 acute hepatitis Toxicity 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 208000017169 kidney disease Diseases 0.000 description 4
- 230000003907 kidney function Effects 0.000 description 4
- 230000003908 liver function Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 206010016654 Fibrosis Diseases 0.000 description 3
- 238000010876 biochemical test Methods 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000007882 cirrhosis Effects 0.000 description 3
- 231100000283 hepatitis Toxicity 0.000 description 3
- 201000007270 liver cancer Diseases 0.000 description 3
- 208000014018 liver neoplasm Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 206010008635 Cholestasis Diseases 0.000 description 2
- 206010019663 Hepatic failure Diseases 0.000 description 2
- 102000002704 Leucyl aminopeptidase Human genes 0.000 description 2
- 108010004098 Leucyl aminopeptidase Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 231100000359 cholestasis Toxicity 0.000 description 2
- 230000007870 cholestasis Effects 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 208000007903 liver failure Diseases 0.000 description 2
- 231100000835 liver failure Toxicity 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- -1 silane compound Chemical class 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical group O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 208000008439 Biliary Liver Cirrhosis Diseases 0.000 description 1
- 208000033222 Biliary cirrhosis primary Diseases 0.000 description 1
- 101100258233 Caenorhabditis elegans sun-1 gene Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010019755 Hepatitis chronic active Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 208000001940 Massive Hepatic Necrosis Diseases 0.000 description 1
- 208000012654 Primary biliary cholangitis Diseases 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 206010052568 Urticaria chronic Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 208000020832 chronic kidney disease Diseases 0.000 description 1
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 1
- 208000024376 chronic urticaria Diseases 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 229940037395 electrolytes Drugs 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 201000009925 nephrosclerosis Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007310 pathophysiology Effects 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000003969 polarography Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002441 uremic toxin Substances 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13693982A JPS5928496A (ja) | 1982-08-06 | 1982-08-06 | 生体試料の分析方法 |
| CA000433955A CA1220700A (en) | 1982-08-06 | 1983-08-05 | Aqueous solution as an eluant used in liquid chromatography |
| DE8383304555T DE3374604D1 (en) | 1982-08-06 | 1983-08-05 | Eluent for liquid chromatography |
| EP83304555A EP0102769B1 (en) | 1982-08-06 | 1983-08-05 | Eluent for liquid chromatography |
| US06/795,275 US4666861A (en) | 1982-08-06 | 1985-11-05 | Aqueous solution as an eluent used in liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13693982A JPS5928496A (ja) | 1982-08-06 | 1982-08-06 | 生体試料の分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5928496A true JPS5928496A (ja) | 1984-02-15 |
| JPH0337708B2 JPH0337708B2 (https=) | 1991-06-06 |
Family
ID=15187067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13693982A Granted JPS5928496A (ja) | 1982-08-06 | 1982-08-06 | 生体試料の分析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5928496A (https=) |
-
1982
- 1982-08-06 JP JP13693982A patent/JPS5928496A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0337708B2 (https=) | 1991-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Eberts et al. | A simplified, colorimetric micromethod for xylose in serum or urine, with phloroglucinol. | |
| JP4356086B2 (ja) | Ｆｔｉｒを使用しての生物学的物質についての迅速試験 | |
| de Macedo et al. | Strong anion determination in biological fluids by capillary electrophoresis for clinical diagnostics | |
| Smith et al. | Time-insensitive fluorescent sensor for human serum albumin and its unusual red shift | |
| Preeti et al. | Estimation of serum creatinine by routine Jaffé’s method and by dry chemistry in icteric and hemolytic serum samples | |
| JP4958272B2 (ja) | アルブミン分子のブラウン運動の拡散係数変化に基づく血清または血漿粘度測定方法及び装置 | |
| JP2004501366A (ja) | 患者の全血、血漿および（または）血清中のアルファ・オキソアルデヒドを検出する方法 | |
| Shahangian et al. | Turbidimetric measurement of total urinary proteins: a revised method | |
| JPS6345066B2 (https=) | ||
| Rice | Improved biuret procedure for routine determination of urinary total proteins in clinical proteinuria | |
| Otnes et al. | Analytical interference by contrast agents in biochemical assays | |
| JPS5928496A (ja) | 生体試料の分析方法 | |
| JPS5940254A (ja) | 生体試料の分析方法 | |
| CN114047263A (zh) | 代谢标志物在制备用于诊断ais的检测试剂或检测物的用途及试剂盒 | |
| JP2002156377A (ja) | 吸光ラベルを用いる尿中疾患マーカータンパク質の迅速・簡易検出方法 | |
| Hilborne et al. | Evaluation of the Behring nephelometer for detection of low level urinary albumin | |
| JP3547879B2 (ja) | フィブリノーゲン中のシアリル糖鎖による生化学検査方法およびフィブリノーゲンの精製方法 | |
| RU2666948C1 (ru) | Способ диагностики мочекаменной болезни | |
| RU2069857C1 (ru) | Способ определения степени поражения почек при нефротическом синдроме | |
| JPS5992349A (ja) | 生体試料の分析法 | |
| Mijušković et al. | Urinary cystatin C as a marker of tubular dysfunction | |
| JPH0337710B2 (https=) | ||
| RU2276366C1 (ru) | Способ диагностики степени тяжести синдрома эндогенной интоксикации у больных с хронической почечной недостаточностью | |
| Benhamza et al. | Comparison of two automated methods for the measurement of glycated hemoglobin HBA1C: ADAMS A1c (ARKRAY) versus Capillarys 2 Flex Piercing®(SEBIA) | |
| Meola et al. | Fluorometric measurement of theophylline. |