JPS5925185B2 - 固相放射免疫測定担体及びその調製及び使用方法 - Google Patents
固相放射免疫測定担体及びその調製及び使用方法Info
- Publication number
- JPS5925185B2 JPS5925185B2 JP54036350A JP3635079A JPS5925185B2 JP S5925185 B2 JPS5925185 B2 JP S5925185B2 JP 54036350 A JP54036350 A JP 54036350A JP 3635079 A JP3635079 A JP 3635079A JP S5925185 B2 JPS5925185 B2 JP S5925185B2
- Authority
- JP
- Japan
- Prior art keywords
- gel
- column
- antigen
- tablet
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000003127 radioimmunoassay Methods 0.000 title claims description 22
- 238000000034 method Methods 0.000 title claims description 21
- 239000007790 solid phase Substances 0.000 title claims description 15
- 238000002360 preparation method Methods 0.000 title description 10
- 239000000499 gel Substances 0.000 claims description 33
- 239000000427 antigen Substances 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 6
- 235000019739 Dicalciumphosphate Nutrition 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- NEFBYIFKOOEVPA-UHFFFAOYSA-K dicalcium phosphate Chemical compound [Ca+2].[Ca+2].[O-]P([O-])([O-])=O NEFBYIFKOOEVPA-UHFFFAOYSA-K 0.000 claims description 3
- 229940038472 dicalcium phosphate Drugs 0.000 claims description 3
- 229910000390 dicalcium phosphate Inorganic materials 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000007791 liquid phase Substances 0.000 claims description 3
- 235000019359 magnesium stearate Nutrition 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000012752 auxiliary agent Substances 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims 1
- 239000000872 buffer Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 102000011923 Thyrotropin Human genes 0.000 description 10
- 108010061174 Thyrotropin Proteins 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 238000011534 incubation Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000010532 solid phase synthesis reaction Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 229910001369 Brass Inorganic materials 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000473945 Theria <moth genus> Species 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000010951 brass Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004452 microanalysis Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/891,650 US4170454A (en) | 1978-03-30 | 1978-03-30 | Process for the preparation of a solid-phase radioimmunoassay support and use thereof |
| US000000891650 | 1978-03-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54143200A JPS54143200A (en) | 1979-11-08 |
| JPS5925185B2 true JPS5925185B2 (ja) | 1984-06-15 |
Family
ID=25398594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP54036350A Expired JPS5925185B2 (ja) | 1978-03-30 | 1979-03-29 | 固相放射免疫測定担体及びその調製及び使用方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4170454A (enExample) |
| JP (1) | JPS5925185B2 (enExample) |
| AU (1) | AU528893B2 (enExample) |
| CA (1) | CA1118346A (enExample) |
| CH (1) | CH642459A5 (enExample) |
| DE (1) | DE2912239C2 (enExample) |
| FR (1) | FR2421381A1 (enExample) |
| GB (1) | GB2017909B (enExample) |
| SE (1) | SE437731B (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4244694A (en) * | 1978-03-31 | 1981-01-13 | Union Carbide Corporation | Reactor/separator device for use in automated solid phase immunoassay |
| US4259207A (en) * | 1979-09-19 | 1981-03-31 | American Hospital Supply Corporation | Suspending medium for immunologic reactions |
| US4623629A (en) | 1983-10-31 | 1986-11-18 | Daniel Kerschensteiner | Solid-phase immunoassay support and method of use thereof |
| US5229268A (en) * | 1986-07-14 | 1993-07-20 | Abbott Laboratories | Method for diagnostic immunoassay by solid phase separation |
| NO892244L (no) * | 1988-06-06 | 1989-12-07 | Photest Diagnostics Inc | Fremgangsmaate til immundiagnostisk bestemmelse. |
| US7108800B2 (en) | 2004-05-14 | 2006-09-19 | Nalco Company | Method for preventing the agglomeration or generation of dust from a particulate material comprising coal |
| JP6023943B2 (ja) | 2009-12-22 | 2016-11-09 | ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ | クロマトグラフィーカラムを乾式充填する方法 |
| WO2025125266A1 (en) * | 2023-12-11 | 2025-06-19 | Cube Biotech Gmbh | Tablets containing fuctionalized agarose particles, method for producing them and use of the tablets |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA990416A (en) * | 1972-04-19 | 1976-06-01 | Sieuwko J. Mantel | Device suitable for use in radioimmunoassay and method of determining the content of a specific hormone in a serum sample |
| SE343949B (enExample) * | 1966-06-02 | 1972-03-20 | Pharmacia Ab | |
| FR1527279A (fr) * | 1966-06-02 | 1968-05-31 | Pharmacia Ab | Procédé et réactif de dosage des protéines et des polypeptides, par exemple des hormones, dans un échantillon aqueux |
| US3996162A (en) * | 1970-02-27 | 1976-12-07 | Nuclear Medical Laboratories, Inc. | Analytical sorbent and method of use |
| AU2919171A (en) * | 1970-05-27 | 1972-11-30 | Pharmacia Ab | A method for carrying out tests, a reagent andan additive for carrying out the method |
| US3659104A (en) * | 1970-06-29 | 1972-04-25 | Univ Jerusalem The | Method of measuring serum thyroxine |
| IL42105A (en) * | 1973-04-25 | 1976-08-31 | Yissum Res Dev Co | Process for determination of triiodothyronine |
| US3925017A (en) * | 1973-05-01 | 1975-12-09 | Wisconsin Alumni Res Found | Preparation of dry, porous gel particles having high water regain for liquid sampling |
| US4070348A (en) * | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
| US4039652A (en) * | 1973-10-11 | 1977-08-02 | Miles Laboratories, Inc. | Column method of immunoassay employing an immobilized binding partner |
| US3980764A (en) * | 1973-11-05 | 1976-09-14 | Curtis Nuclear Corporation | Polymeric competitive protein binding adsorbents for radioassey |
| US3953172A (en) * | 1974-05-10 | 1976-04-27 | Union Carbide Corporation | Method and apparatus for assaying liquid materials |
| US3947352A (en) * | 1974-05-31 | 1976-03-30 | Pedro Cuatrecasas | Polysaccharide matrices for use as adsorbents in affinity chromatography techniques |
| US4061466A (en) * | 1974-10-16 | 1977-12-06 | Ingvar Gosta Holger Sjoholm | Biologically active composition and the use thereof |
| US4018883A (en) * | 1975-03-10 | 1977-04-19 | Nichols Institute For Endocrinology | Thyroxine (T4) radioimmunoassay |
| JPS5267395A (en) * | 1975-12-01 | 1977-06-03 | Japan Exlan Co Ltd | Opening prcess for high density filling column in liquid chromatography |
-
1978
- 1978-03-30 US US05/891,650 patent/US4170454A/en not_active Expired - Lifetime
-
1979
- 1979-03-15 CA CA000323523A patent/CA1118346A/en not_active Expired
- 1979-03-28 DE DE2912239A patent/DE2912239C2/de not_active Expired
- 1979-03-29 JP JP54036350A patent/JPS5925185B2/ja not_active Expired
- 1979-03-29 GB GB7910983A patent/GB2017909B/en not_active Expired
- 1979-03-29 SE SE7902810A patent/SE437731B/sv unknown
- 1979-03-29 AU AU45580/79A patent/AU528893B2/en not_active Ceased
- 1979-03-29 FR FR7907903A patent/FR2421381A1/fr active Granted
- 1979-03-29 CH CH293879A patent/CH642459A5/fr not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| GB2017909B (en) | 1983-01-19 |
| GB2017909A (en) | 1979-10-10 |
| CH642459A5 (fr) | 1984-04-13 |
| JPS54143200A (en) | 1979-11-08 |
| AU4558079A (en) | 1979-10-04 |
| SE437731B (sv) | 1985-03-11 |
| FR2421381A1 (fr) | 1979-10-26 |
| FR2421381B1 (enExample) | 1984-03-16 |
| US4170454A (en) | 1979-10-09 |
| DE2912239A1 (de) | 1979-10-04 |
| SE7902810L (sv) | 1979-10-01 |
| DE2912239C2 (de) | 1982-12-30 |
| CA1118346A (en) | 1982-02-16 |
| AU528893B2 (en) | 1983-05-19 |
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