JPS59153150A - Optical analytic apparatus of minute amount of component - Google Patents

Abstract

PURPOSE:To attain to miniaturize the titled apparatus by making it possible to freely set the positional relation of a wavelength selecting optical system and a cell while facilitating assembling/adjusting work, by irradiating the cell through an optical fiber. CONSTITUTION:One end part of an optical fiber 14 is secured to the light output part 2a of a wavelength selecting optical system 2 and the other end part thereof is inserted through the piercing orifices of the gasket bodies 12, 12 of a T-shaped joint 10 to be arranged in one end of a cell 7 so as to leave the gap between the inner peripheral wall of said cell 7 and the optical fiber 14. By this mechanism, ultraviolet rays having predetermined wavelenghts outputted from the wavelength selecting optical system 2 are introduced into the cell 7 through the optical fiber 14 to irradiate the specimen liquid supplied into the cell 7 from a liquid supply tube 9. In this case, the width of luminous flux is not restrained in the light output part 2a, the luminous flux has a large diameter as compared with that of the optical fiber 14 and adjusting work becomes extremely easy.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an optical trace component analyzer that analyzes components by irradiating a sample with light of a predetermined wavelength.

An example of this type of device is an ultraviolet fluorescence photometry device used as an analysis device for liquid chromatography, which is generally configured as follows. That is, ultraviolet rays emitted from an ultraviolet lamp are sorted into predetermined wavelength components by a wavelength selection optical system equipped with a filter, a diffraction grating 1 reflecting mirror, a slit, etc., and are made into a narrow beam of light. A cell was placed in the light output section, and the sample for analysis flowing through the cell was irradiated with ultraviolet light.

However, since the cell is extremely small with an internal volume on the order of microliters, it is necessary to use a condenser lens and a mirror to reliably and effectively irradiate the sample in the cell with the light beam output from the wavelength selection optical system.

It was necessary to further narrow down the luminous flux using a slit or the like, and to strictly align the luminous flux with the cell. For this reason, although the cell originally has a relatively complex structure with pipes for sample supply and discharge, it must be constructed in a form that is integrated with a wavelength selection optical system, making it difficult to assemble and adjust. Moreover, since there is no degree of freedom in respect of each other between the wavelength selection optical system and the cell, there is a problem in that wasted space is likely to be created within the device, and as a result, the entire device becomes larger.

The present invention has been developed in view of the above-mentioned 13 circumstances, and its purpose is to irradiate light from a wavelength selective optical system to a cell via an optical fiber, thereby enabling a wavelength selective optical system to be irradiated to a cell. To provide an optical trace component analyzer in which the positional relationship with a cell can be freely set, the accuracy of assembly and adjustment is not so strictly required, and the overall size can be reduced.

The following is a first example in which the present invention is applied to an ultraviolet spectral photometry device.
An example will be described with reference to FIGS. 1 and 2. 1
2 is an ultraviolet lamp as a light source, and 2 is a wavelength selection optical system. This wavelength selection optical system 2 uses a filter 6 to remove unnecessary wavelength components from the ultraviolet rays emitted from the ultraviolet lamp 1, and irradiates the concave diffraction grating 5 through a slit 4. , the ultraviolet rays of a predetermined wavelength that have been separated and reflected by the concave diffraction grating 5 are focused on the light output section 2a. On the other hand, it is a 6-cell unit, and as shown in detail in FIG. 2, it is equipped with a self formed by a right cylindrical quartz tube. Sef L/7
A liquid supply pipe 9 is connected to one end via a Teflon tube 8, and this liquid supply pipe 9 is connected, for example, to the outlet side of a column of a liquid chromatograph (not shown). 10 is a T-shaped joint, which has through holes 1 at both ends of the tube body 11, respectively.
2B is screwed onto the plug body 12, and a drainage hole 11& is formed in the peripheral wall of the tube body 11.
The other end of the self is liquid-tightly fitted into the through hole 12B, and a drain pipe 13 is connected to the drain hole 11a. 14
is an optical fiber, one end of which is fixed to the light output part 2a of the wavelength selection optical system 2, and the other end is connected to the through hole 1'l a of each stopper 12, 12 of the T-shaped joint 10.
, jZ Insert the inside of the light irradiation part 7a of C/L/7
That is, it is arranged within the other end of the self with a gap between it and the inner circumferential wall. As a result, the ultraviolet rays of a predetermined wavelength outputted from the wavelength selection optical system 2 are transferred to the optical fiber 14.
The sample liquid is introduced into the lua through the liquid supply tube 9 and supplied into the cell section from the liquid supply pipe 9 so that the sample liquid is irradiated with light L7.

In addition, the through hole 1 of the other stopper 12 of the T-shaped joint 10
2a is a portion through which an optical fiber 14 is passed and is sealed liquid-tightly, so that the sample liquid that has flowed into the T-shaped joint 10 from the luer flows out to the drain pipe 15 side. Reference numeral 15 denotes a concave mirror, which is disposed so that its reflective surfaces face each other along the direction fv7, and reflects and condenses the t-spectral light generated from the sample liquid irradiated with ultraviolet rays in a predetermined direction.

The fluorescent light reflected by the concave mirror 15 or directly emitted from the self-internal force passes through a filter 16 and a slit 17 provided on the opposite side of the concave mirror 15 to a photomultiplier tube 18.
and is converted into an electrical signal corresponding to the luminous intensity ζ of the fluorescent light. In addition, 19 is a /\-Fumifu placed in the optical path of the ultraviolet rays of the wavelength selection optical system 2;
A portion of the ultraviolet rays is reflected and irradiated to the photomultiplier tube 21 through the slit 20, and an electric signal corresponding to the intensity of the ultraviolet rays is output. The output signal of this photomultiplier tube 21 is inputted to a differential amplifier (not shown) having the same number as the output signal of the self-side photomultiplier tube 18, for example, and cancels output fluctuations of the ultraviolet radiation 1. Or the power supply circuit of ultraviolet lamp 1 (
Ultraviolet lamp 1
Rub the output for a certain amount of time.

According to the above configuration, if one end of the optical power Rufaino<-14 is fixed to the light output section 2ai of the wavelength selection optical system 2, the ultraviolet rays from the wavelength selection optical system 2 can be removed from the light irradiation section 7a of the wavelength selection optical system 2. can be reliably guided. Here, in the light output section 2aζ of the wavelength selection optical system 2, as in the conventional case,
Since the beam width is not limited by the slit, the beam is larger than the diameter of the optical fiber 14, and therefore the positional accuracy 1d of the optical force applied to the optical output section 2a is significantly rougher than before. This greatly facilitates assembly and adjustment work. Moreover, since the optical fiber 14 is bendable, the cell unit 6
can be arranged in a free positional relationship with respect to the wavelength selection optical system 2, thereby preventing wasted space within the device and making it possible to downsize the entire device. Furthermore, in the conventional configuration in which the luminous flux is focused using a slit-nod, if the cell is to be made smaller, the opening width of the slit must be made extremely narrow, which ultimately limits positional accuracy. However, according to this embodiment, the optical sophino (-1
4, it is possible to narrow the ζζ beam without considering positional accuracy, and accordingly, the volume of the cell can be reduced accordingly to improve the resolution of measurement.

Next, a second embodiment in which the present invention is applied to an ultraviolet absorption photometry device will be described with reference to FIGS. 22
is an ultraviolet lamp as a light source; 23 is a wavelength selection optical system; this wavelength selection optical system 23 reflects the ultraviolet rays emitted from the ultraviolet lamp 22 by a concave mirror 24 and a plane mirror 25, and irradiates the diffraction grating 26 through a slit 26; The ultraviolet rays of a predetermined wavelength that have been separated and reflected by the diffraction grating 26 are reflected by a concave mirror 27 and focused on the light output section 23a. As shown in FIG. 4, the cell 28 is formed of a right cylindrical quartz tube, and the two ends of the cell 29 are covered with Teflon tubes 50. ,
A lees/liquid pipe 31 and a drain pipe 32 are connected through a pipe 30, and the liquid supply pipe 31 is connected to a fliE outlet (1111) of a column of liquid chromatography (not shown), and the drain pipe 52 is connected to a drain pipe (not shown). 35 is an optical fiber, which is composed of, for example, four single wires as shown in FIG. They are fixed to each other by a binding member 34, and on the other end side, each single wire is lined up in a row and a second
are fixed to each other by binding members 55. and,
The 10M eastern part 34 of this optical fiber 63
is placed in the light output section 23a of the wavelength selection optical system 23, and the second
The binding member 65 is attached to the light irradiation part 29a of the cell 29, that is, the cell 2
90 is tightly fixed to a predetermined portion of the peripheral wall. By kneading, the ultraviolet rays of a predetermined wavelength output from the wavelength selection optical system 23 are passed through the optical fiber 33 to 7/l/
29 is irradiated by passing through the peripheral wall. 36 is a photomultiplier tube, which is disposed opposite to the light irradiation part 29a of the cell 29 (111), and is connected to the optical fiber 63.
The ultraviolet rays irradiated from 77 to 29 and transmitted through the sample liquid in the cell 29 are received, thereby measuring the absorbance of the sample liquid. Note that 57 is an /X-Fumifu located along the optical path of the ultraviolet edge of the wavelength selection optical system 23, which reflects a part of the ultraviolet light from the wavelength selection optical system 23 and passes it through the slit 38 to, for example, a photomultiplier tube 69. The device outputs a drowsiness signal depending on the intensity of the ultraviolet rays. Then, from this output signal ζ, the ultraviolet lamp 2 is
This is to cancel the output fluctuation of 2 or to make the output constant.

Also in the above configuration, the light output section 2 of the wavelength selection optical system 26
3a, unlike the conventional case, the luminous flux of the violet 9I line is not narrowed down by a slit, so the luminous flux is large, and therefore the positioning accuracy when fixing the first binding member 34 of the optical fiber 35 to the optical fiber 35 to the optical output part 23a is lower than that of the conventional one. It does not require as much rigor as the previous method, making assembly and adjustment work extremely easy.

In this way, even if the first binding member 34 of the optical fiber 36 is arranged with rough precision with respect to the light output part 23a, the ultraviolet edge can be reliably guided to the light irradiation part 29a of the cell 29, and the slit can be Of course, when used, the light beam can be narrowed down to the diameter of the optical fiber 33. Moreover, the light beam can be formed into any shape by combining a plurality of optical fibers 33 or the like. In addition, the cell 29
Since the positioning accuracy between the wavelength selection optical system 23 and the wavelength selection optical system 23 can be narrowed down sufficiently without considering the tl, the volume of the cell 29 can be reduced accordingly, thereby improving the measurement resolution. I can do it. Furthermore, since the cell 29 can be arranged in a free positional relationship with respect to the wavelength selection optical system 23, it is also possible to downsize the entire device.

Note that the present invention is not limited to the embodiments described above and shown in the drawings. For example, if an optical fiber in which the refractive index of the core part and the cladding part is continuously changed is used, the optical fiber can be It is possible to irradiate the cell without unnecessarily diffusing the light beam irradiated from the fiber, which can further improve the resolution of measurement, and the shape of the cell is not limited to a cylindrical shape, within the scope of the main point. It can be implemented with various modifications.

As described above, the present invention provides an optical fiber with one end facing the light output section of the wavelength selection optical system on the light source side,
The feature is that the other end of the optical fiber is arranged so as to face the light irradiation part of the cell, and as a result, the positional relationship between the wavelength selection optical system and the cell can be completely free, and one end of the optical fiber can be used as the light output part. On the other hand, even when placed with rough positioning accuracy, the light beam can be made sufficiently thin and guided to the cell reliably.
As a result, assembly and adjustment work can be made extremely easy, and the entire device can be made smaller.

[Brief explanation of drawings]

1 and 2 show a first embodiment of the present invention, FIG. 1 is an overall schematic configuration, FIG. 2 is an enlarged sectional view of a cell unit, and FIGS. 3 to 5 are in accordance with the present invention. A second example of
FIG. 3 is a schematic diagram of the overall configuration, FIG. 4 is an enlarged sectional view of the cell unit, and FIG. 5 is a perspective view of the optical fiber. In the figure, 1 and 22 are ultraviolet lamps (light sources), 2.23 are wavelength selection optical systems, 2a, 23& are light output parts, 6.28 are cell units, 7.29 are cells, and 7a. 29a is a light irradiation section, 14.33 is an optical fiber, and 18, 21.56.59 are photomultiplier tubes. 6 jPl 1 Figure 2 Figure jlli3 Figure

Claims (1)

[Claims] 1. In a device that analyzes sample components in a cell by irradiating light of a predetermined wavelength from a light source into the cell, one end of the optical fiber faces the light output section of the wavelength selection optical system on the light source side, An optical trace component analyzer, characterized in that the other end is arranged so as to face the light irradiation part of the cell.