JPS5872049A - Selective dyeing apparatus for electrophoresis pattern - Google Patents

Abstract

PURPOSE:To improve the treatment accuracy and efficiency of selective dyeing, by carrying out dyeing and decoloring treatments in a single treatment vessel automatically and continuously. CONSTITUTION:A dyeing liquid in a dyeing liquid tank 29 is introduced into a treatment vessel 15 by rotating a pump 31 to a normal direction and a sheet- shaped supporting body after treating by electrophoresis is inserted into the vessel 15. The dyeing liquid is returned to the tank 29 at the point of time of finishing dyeing treatemt. Already used decoloring liquid in the first decoloring liquid vessel 26 is sent into the vessel 15 by a pump 36 and the first decoloring treatment is carried out and the decoloring liquid is introduced into a waste liquid tank 32 by a pump 34. New decoloring liquid in the second decoloring liquid tank 37 is charged into the vessel 15 by a pump 39 and the decoloring treatment is carried out then, the decoloring liquid is transferred to the tank 26 as an old liquid by a pump 28. A series of these actions is carried out successively by an electric signal of a preset timer.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an electrophoretic pattern selective staining apparatus particularly suitable for creating electrophoretic patterns of serum proteins.

For the purpose of human health management, it is common practice to create electrophoretic patterns of serum proteins.

In this case, as shown in FIG. 1, the serum sample 2 on the support 1 is applied dropwise onto a sheet-like support 1 made of a cellulose acetate membrane, etc., and subjected to electrophoresis treatment. expands to a length of 16 to 50 m+n, creating a striped latent image depending on the protein content of the serum sample. This latent image becomes a t-colored striped pattern as shown in FIG. 2 by the subsequent selective staining process, and by analyzing the density distribution of this striped pattern 3, the quality of the serum sample is determined.

According to the electrophoretic pattern selective staining apparatus of the present invention, the staining process and decolorizing process of the selective staining process are automatically and continuously performed in a single liquid tank, thereby significantly improving process accuracy and process efficiency. can do. Also,
The utilization rate of the decolorizing solution can be increased.Next, the outpost apparatus of the present invention will be explained along with embodiments shown in the drawings.

In FIG. 3, the electrophoresis processing tank 4 has an internal space partitioned by first and second partition plates 5 and 6, and a first and a 20th liquid reservoir 8, and a platinum wire tube 8 inside the electrophoresis processing tank 4. First and second electrodes 9 and 10 are laid down, respectively. Further, the lower end of the first water-absorbing paper 11 hanging down from the top of the first partition plate 6 is immersed in the buffer solution 12 in the first liquid reservoir 7, and the lower end part of the first water-absorbing paper 11 hanging down from the top of the second partition plate 6 is immersed in the buffer solution 12 in the first liquid reservoir 7. The lower end of the second water-absorbing paper 13 is immersed in the buffer solution 14 in the second liquid reservoir 8. Then, the sheet-like support 1 made of cellulose acetate membrane or agar membrane is attached to the top of each of the first and second partition plates 6.6.
It is placed on 11.130 pieces of water-absorbing paper.

Therefore, the first and second water-absorbing papers 11.13 and the support 1 are kept in a moist state, and the support 1"
2. Apply serum sample 2 dropwise to the first and second electrodes 9.
, 10, the electrophoresis process is started. Then, an electrophoretic pattern of serum proteins corresponding to the protein content of the serum sample is formed as a latent image on the support 1, and this is selectively stained to obtain a colored electrophoretic pattern as shown in FIG.

The selective dyeing process consists of a dyeing process and a decolorizing process,
Conventionally, both processes were performed in separate processing tanks. That'-
1. In order to ensure complete decolorization treatment, approximately four decolorization treatment tanks are provided for one dyeing treatment tank, and the sheet-like support after dyeing treatment is transferred from the first decolorization treatment tank to the fourth decolorization treatment tank. It was common practice to move from one stage to the next. For this reason, there is a drawback that not only does it require considerable effort to transfer the support, but also that the membrane surface of the support is easily damaged.

In the apparatus of the present invention, the dyeing process and the decolorizing process can be carried out automatically and continuously in the processing tank 15 configured as shown in FIG. :Resolved.

The processing tank 15 has a bottom wall 16 that is low in the front and high in the back, and has a first wall at the bottom of the front wall. 2nd and 3rd supply 4J1 water 1'' 17.18
．． It has 19. There is also a fourth wall on the top of the front wall.
51st 6th and 7th water supply and drainage ports 20.21.22.2
3, an eighth water supply υ1 water port 24 is provided at the bottom of the left and right 10 side walls, and a ninth water supply and drain port 25 is provided at the bottom of the right side wall.

A first electric pump 28 is connected to a pipe 2 provided between the first water supply and drainage port 17 and the first decolorizing liquid tank 26, and a first electric pump 28 is connected between the second water supply and drainage port 18 and the dyeing liquid tank 29. A second electric pump 31 is connected to a pipe 30 that connects the two, and a third electric pump 34 is connected to a pipe 33 that connects the third water supply port 19 and the waste liquid tank 32. . The fourth and seventh water supply and drainage pipes 1120.
A fourth electric pump 36 is connected to the nozzle 35 that connects the fifth and sixth water supply/drainage ports 21 and 22 to the first decolorizing liquid tank 37. 1 to the pipe 38 connecting between. A fifth electric pump 39 is connected to the eighth water supply port 24 and the ninth water supply and drainage port 2.
A sixth electric pump 41 is connected to a pipe 40 connecting between the pump and the pump.

During the dyeing process, first, the second electric pump 31 is rotated in the forward direction to transfer the dyeing solution in the dyeing solution tank 29 to the processing tank 1.
At the same time, the sixth electric pump 41 is rotated in the forward direction to circulate the dyeing solution in the processing tank 16. and,
The sheet-like support 1 after the electrophoresis treatment is inserted into the treatment tank 15 and suspended in the staining solution. However, when dyeing a plurality of sheet-like supports at once, a suitable spacer made of wire mesh or the like is interposed between the supports to prevent them from overlapping each other. As the dye of the dyeing liquid, an acidic dye such as Bonso 3R can be used, and the time required for dyeing by dipping is 1 minute 30 seconds to 3 minutes.

When the dyeing process is completed, the second electric pump 31 is rotated in the opposite direction to transfer the dyeing solution in the processing tank 15 to the dyeing solution tank 2.
Return to 9. Then, the fourth electric pump 36 is rotated to transfer the decolorizing liquid in the first decolorizing liquid tank 26 to the processing tank 16.
The sample is sent inside and subjected to the first decolorization process. Although RL sagar acid can be used as the decolorizing liquid, the decolorizing liquid t1 in the first decolorizing liquid tank 26 is an old liquid that has been used once, and is highly concentrated due to the staining liquid mixed in. The first decolorization process is set for a short time, and the third electric pump 34 is rotated every i'i'l to guide the decolorization liquid in the processing tank 15 into the waste liquid tank 32.

Next, the fourth electric pump 36 is rotated again to pour the old solution in the first decolorizing solution tank 26 into the processing tank 15.
Second decolorization process - This is done relatively carefully. After this treatment, the decolorizing liquid in the processing tank 16 is transferred to the waste liquid tank 32, and then the first and sixth electric pumps 39 are rotated, and the second
The new decolorizing liquid in the decolorizing liquid tank 37 is poured into the processing tank 15. Then, a third decolorization process is performed, but since the decolorization liquid in the processing tank 15 becomes slightly contaminated during this process, the first electric pump 28 is rotated to remove the old liquid from the process tank 15. The liquid is then transferred into the first decolorizing liquid tank 26. Thereafter, the fifth electric pump 39 is rotated again to pour the new decolorizing liquid in the second decolorizing liquid tank 37 into the processing tank 15, and a fourth decolorizing process is performed. Then, the decolorizing liquid in the processing tank 15 is transferred into the first decolorizing liquid tank 26 by the first electric pump 28, thereby completing a series of decolorizing processes.

Note that the sixth electric pump 41 for circulation continues to rotate during the decolorization process. Further, the above-described series of operations are sequentially performed by electrical signals from a timer that performs these operations. If there is a risk that, for example, the decolorizing solution in the processing tank 15 will leak into the staining solution tank 29 through the second electric pump 31, a valve can be installed on the pipe, but immediately before stopping each electric pump, the pipe should be closed. The above-mentioned valve is not required when air is allowed to flow inside. Furthermore, when installing the first decolorizing liquid tank 26 and the waste liquid tank 32 below the processing tank 15, the first electric pump 28 and the third electric pump 33 can be replaced with a solenoid valve. 1
The electric pump 39 installed in the decolorizing liquid tank 26 or 2 and the second decolorizing liquid tank 3 on the side of the processing tank 15 can be replaced with a solenoid valve.

As described above, when the selective staining apparatus of the present invention is used, the staining process and the decolorizing process can be performed automatically and continuously in a single processing tank, which improves the processing accuracy and processing efficiency of selective staining. can be significantly improved. 4. Since the decolorizing liquid is rationally reused, there is an advantage that the utilization rate of the decolorizing liquid can be increased without reducing the processing temperature.

[Brief explanation of drawings]

Figure 1 is a plan view showing the relationship between a sheet-like support and a serum sample coated on the support, Figure 2 is a plan view showing electrophoresis and patterns of serum proteins, and Figure 3 is a plan view showing electrophoresis. FIG. 4 is a partially cutaway perspective view of a processing tank, and FIG. , , Serum sample, 15 , , Processing tank, 17.1B, 19
,20゜21 ,22 ,23 ,24 ,25 ,,
,,,,supply JJI water],26,,,,,,first decolorizing liquid tank,28,31°34,36,39,4
1, +, electric pump, 290...staining liquid tank, 32, waste liquid tank, 37... second decolorizing liquid tank.

Claims (1)

[Claims] A staining liquid tank, a processing tank connected to the tank via an electric pump, first and second decolorizing liquid tanks connected to the processing tank via respective electric pumps or solenoid valves, and a timer. The timer includes an electric signal for guiding the staining liquid in the staining liquid tank into the processing tank, an electric signal for guiding the staining liquid in the processing tank into the staining liquid tank, and the timer. an electric signal for guiding the decolorizing liquid in the first decolorizing liquid tank into the processing tank; an electric signal for discharging the decolorizing liquid in the processing tank to the outside; and an electric signal for discharging the decolorizing liquid in the second decolorizing liquid tank to the outside of the tank. A signal generating means for sequentially generating an electric signal for guiding the decolorizing liquid into the processing tank and an electric signal for guiding the decolorizing liquid in the processing tank into the first decolorizing liquid tank. An electrophoretic pattern selective staining device featuring: