CN218297766U

CN218297766U - Biopolymer substance dyeing instrument

Info

Publication number: CN218297766U
Application number: CN202222085119.6U
Authority: CN
Inventors: 张硕; 曹飞婷; 方利
Original assignee: Changzhou Boyi Biotechnology Co ltd
Current assignee: Changzhou Boyi Biotechnology Co ltd
Priority date: 2022-08-09
Filing date: 2022-08-09
Publication date: 2023-01-13
Anticipated expiration: 2032-08-09

Abstract

The utility model belongs to the technical field of the biotechnology technique and specifically relates to a biological macromolecular substance dyeing instrument is equipped with dyeing pond and upper cover, the upper cover lid closes on the dyeing pond, the bottom of upper cover is fixed with negative plate and division board, the negative plate is located between upper cover and the division board, and leaves the clearance between negative plate and the division board, the bottom in dyeing pond is fixed with the positive plate, the positive plate top is fixed with another division board, and leaves the clearance between this division board and the positive plate, all be equipped with the hole system that is used for the liquid flow on the division board, form the clearance that is used for holding the gel between two division boards when the upper cover lid closes, adopt the electric field to dye, the gel is the slice, and the gel does not take place the condition such as curling, float, can save time greatly, adopts unified instrument and operation flow to dye, increases the repeatability of result effectively, avoids the uncertainty that human factor brought, adopts balanced processing to shorten the dyeing time to strengthen the dyeing effect.

Description

Biopolymer substance dyeing instrument

Technical Field

The utility model belongs to the technical field of biotechnology technique and specifically relates to a biological macromolecular material dyeing instrument.

Background

Biopolymer polymers having different charges can be separated by electrophoresis on a medium, which is usually referred to as gel medium, and includes but is not limited to polyacrylamide and agarose. The polymer as used herein includes proteins, nucleic acids, polysaccharides, and the like. For example, polyacrylamide gel electrophoresis, which is used to separate and detect proteins, is a method in which proteins are arranged on a gel according to a certain rule after a protein sample is electrophoresed on polyacrylamide gel. Hereinafter, such a dye is referred to as a gel, and the substance to be dyed is a protein. The purpose of the staining is to apply a method of staining the protein bands on the gel with a dye that allows the protein bands to be distinguished. For polyacrylamide gel staining dyes, the main ones include, but are not limited to, amino black, coomassie brilliant blue G250, coomassie brilliant blue R250, and the like. For dyeing polyacrylamide gels, it is common to immerse the gel in a liquid with some additional shaking. Conventional dyeing steps are classified into fixing (fix), dyeing (stain), and decoloring (destain), although some modifications may omit steps other than dyeing.

The entire process is usually completed for non-heated dyeing in a time of 2 hours to several days, which is excessively long. The heating dyeing process usually takes 30 minutes to 1 hour, and the heating dyeing has the disadvantages that the organic solvent or acid substances of the dyeing solution or the decoloring solution volatilize when the dyeing solution or the decoloring solution is heated to boiling, the environment is not friendly, and the gel is broken by heating. In addition, since the thermal dyeing is performed manually, the effect of thermal dyeing cannot be guaranteed to be the same as that of thermal dyeing.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model provides a biomacromolecule dyeing instrument.

The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a biological macromolecular substance dyeing instrument, is equipped with dyeing pond and upper cover, the upper cover lid closes on the dyeing pond, the bottom of upper cover is fixed with negative plate and division board, the negative plate is located between upper cover and the division board, and leaves the clearance between negative plate and the division board, the bottom in dyeing pond is fixed with the positive plate, the positive plate top is fixed with another division board, and leaves the clearance between this division board and the positive plate, all be equipped with the hole system that is used for the liquid to flow on the division board, form the clearance that is used for holding the gel between two division boards when the upper cover lid closes.

According to the utility model discloses a further embodiment, further include, be equipped with the shell, the dyeing pond is fixed on the top surface of shell, one side of upper cover is connected with one side of dyeing pond is articulated.

According to the utility model discloses a further embodiment, further include, the dyeing pond respectively establishes one in the left and right sides of shell, the upper cover is connected with the left and right sides face of shell is articulated respectively.

According to the utility model discloses a further embodiment, further include, be equipped with three solution on the shell and pour into interface and a waste liquid discharge interface, the solution is poured into the interface and is used for respectively pouring into balanced liquid, dyeing liquid and decoloration liquid in the dyeing pond, waste liquid discharge interface is used for discharging the waste liquid after handling in the dyeing pond.

According to the utility model discloses a further embodiment, further include, interface and waste liquid discharge interface are irritated by a peristaltic pump and diverter valve control to solution, the diverter valve is used for switching the liquid kind and the discharge waste liquid of irritating.

According to the utility model discloses a further embodiment, further include, be equipped with control panel on the shell, control panel is touch display screen control system.

The beneficial effects of the utility model are that, adopt the electric field to dye, the gel is the slice, the gel does not take place to curl, the condition such as float, can save time greatly, it is balanced, the dyeing, the decoloration step all can be controlled within 5 minutes, effectively avoid the heating of dyeing in-process volatile materials to volatilize, and operate in airtight system, can effective control experimental environment, adopt unified instrument and operation flow to dye, increase the repeatability of result effectively, avoid the uncertainty that artificial factor brought, adopt balanced processing to shorten the dyeing time, and strengthen the dyeing effect, and convenient operation.

Drawings

The present invention will be further explained with reference to the drawings and examples.

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a schematic structural view of the utility model when the upper cover is opened;

FIG. 3 is a schematic partial sectional view of the dyeing tank with the upper lid closed;

fig. 4 is an enlarged view at X in fig. 3.

In the figure, a dyeing tank 1, an upper cover 2, a positive plate 3, a negative plate 4, a separation plate 5, a hole system 51, a shell 6, a solution filling interface 7 and a control panel 8 are arranged.

Detailed Description

Fig. 1-4 are the structural schematic diagram of the utility model, a biological macromolecular substance dyeing instrument is equipped with dyeing pond 1 and upper cover 2, upper cover 2 lid closes on dyeing pond 1, the bottom of upper cover 2 is fixed with negative plate 4 and division board 5, negative plate 4 is located between upper cover 2 and division board 5, and leaves the clearance between negative plate 4 and division board 5, the bottom of dyeing pond 1 is fixed with positive plate 3, positive plate 3 top is fixed with another division board 5, and leaves the clearance between this division board 5 and positive plate 3, all be equipped with the hole system 51 that is used for the liquid to flow on the division board 5, form the clearance that is used for holding the gel between two division boards 5 when upper cover 2 lid closes.

Placing a gel medium to be dyed for separating high molecular polymers in an electric field, and dyeing the gel medium by using the electric field. The positive electrode plate 3 and the negative electrode plate 4 form an electric field in the solution when energized.

The tank body of the dyeing tank 1 is made of corrosion-resistant materials, and the upper cover 2 and the dyeing tank 1 can be sealed in a closed mode. The material of the positive plate 3 is titanium or titanium alloy, and the surface of the positive plate is prevented from being corroded by electricity by the inert metal plating. The negative plate 4 is made of stainless steel or titanium alloy, and the negative plate 4 is not generally subjected to plating treatment. The gel placing area is arranged between the two isolation plates 5, the thickness of the area between the two isolation plates is about 3mm, the thickness of the gel is 1-1.5mm, the space can effectively control the situations that the gel does not curl, float and the like, the isolation plates 5 are made of corrosion-resistant materials, and the solution can effectively immerse the gel due to the porous structure.

The middle part of the upper cover 2 protrudes downwards and extends into the dyeing tank 1. So that the dyeing liquid can contact the liquid without filling the whole dyeing tank 1 with the dyeing liquid and the negative plate 4.

Preferably, a shell 6 is arranged, the dyeing tank 1 is fixed on the top surface of the shell 6, and one side of the upper cover 2 is hinged with one side of the dyeing tank 1. When the upper cover 2 is turned over by the structure, the negative plate 4 and the isolation plate 5 are still positioned above the dyeing tank 1, and the solution is not easy to drip or flow out.

Preferably, the dyeing tank 1 is respectively provided with one on the left side and the right side of the shell 6, and the upper cover 2 is respectively hinged with the left side and the right side of the shell 6.

The upper cover 2 is provided with a handle for facilitating the opening of the upper cover.

Preferably, the housing 6 is provided with three solution filling ports 7 and a waste liquid discharge port, the solution filling ports 7 are respectively used for filling the balance liquid, the dyeing liquid and the decoloring liquid into the dyeing tank 1, and the waste liquid discharge port is used for discharging the waste liquid treated in the dyeing tank 1.

Because the gel is subjected to an electrophoresis process before dyeing, or the gel is initially designed to meet the electrophoresis process, the gel must have an electrophoresis buffer system besides a crosslinked polymer network substance for supporting, wherein the electrophoresis buffer system substance has a large amount of residual buffer substances, such as Tris, glycine, mops and the like, in the gel after the electrophoresis is finished, and the presence of the buffer substances in the gel can interfere with the dyeing of the dye and increase the difficulty of decoloring, or the dyed polymer sample can be distinguished by decoloring treatment. Furthermore, for the biopolymer to be dyed, a certain degree of treatment thereof may increase the degree of its coloration by the dye.

Before dyeing, balance liquid is injected into the dyeing tank 1, the balance liquid can be ammonium sulfate, urea, phosphoric acid and other substances capable of reducing coloring of non-dyeing areas, charged particles of the substances in the solution can move, the substances are contained in the gel, and original buffer substances possibly interfering dyeing of the gel are left in the solution to a certain extent. By balancing the electric field prior to staining, exclusion of staining negatives adds staining negatives. The substances for treating the substance to be dyed to improve the dyeing effect are also added into the electric field and the substances in the conventional fixing (fix) step, so that the aim of optimizing the dyeing environment is fulfilled.

Preferably, the solution filling port 7 and the waste liquid discharging port are controlled by a peristaltic pump and a switching valve, and the switching valve is used for switching the type of the filled liquid and discharging the waste liquid.

Preferably, a control panel 8 is arranged on the housing 6, and the control panel 8 is a touch display screen control system.

The main control parameters include the type of liquid to be filled, the volume of the liquid to be filled, the waste liquid to be discharged, the power-on time of the electrode plate, the end, the warning and other information. The steps can be combined to form an immobilized program, and the control system can store the immobilized program for daily use.

The foregoing description is intended to be illustrative rather than limiting, and it will be appreciated by those skilled in the art that many modifications, variations or equivalents may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. The utility model provides a biological macromolecular substance dyeing instrument, characterized by is equipped with dyeing pond (1) and upper cover (2), upper cover (2) lid closes on dyeing pond (1), the bottom of upper cover (2) is fixed with negative plate (4) and division board (5), negative plate (4) are located between upper cover (2) and division board (5), and leaves the clearance between negative plate (4) and division board (5), the bottom of dyeing pond (1) is fixed with positive plate (3), positive plate (3) top is fixed with another division board (5), and leaves the clearance between this division board (5) and positive plate (3), all be equipped with hole system (51) that are used for the liquid to flow on division board (5), form the clearance that is used for holding the gel between two division boards (5) when upper cover (2) lid closes.

2. The apparatus according to claim 1, wherein a housing (6) is provided, the staining bath (1) is fixed on the top surface of the housing (6), and one side of the upper cover (2) is hinged to one side of the staining bath (1).

3. The biopolymer substance staining instrument of claim 2, wherein the staining bath (1) is provided with one on each of the left and right sides of the housing (6), and the upper cover (2) is hinged to each of the left and right sides of the housing (6).

4. The biopolymer substance dyeing instrument according to claim 2, wherein the housing (6) is provided with three solution filling ports (7) and a waste liquid discharging port, the solution filling ports (7) are respectively used for filling the balancing liquid, the dyeing liquid and the decoloring liquid into the dyeing tank (1), and the waste liquid discharging port is used for discharging the waste liquid treated in the dyeing tank (1).

5. The apparatus according to claim 4, wherein the solution inlet port (7) and the waste liquid outlet port are controlled by a peristaltic pump and a switching valve for switching the type of liquid to be introduced and the waste liquid to be discharged.

6. The biopolymer substance staining instrument of claim 2, wherein the housing (6) is provided with a control panel (8), and the control panel (8) is a touch display screen control system.