JPS59141050A - Electrophoretic apparatus - Google Patents

Abstract

PURPOSE:To well achieve plural times of electrophoretic treatments, by constituting the titled apparatus so that a buffer solution is flowed into a liquid sump along a water absorbing sheet while flowed out through a large number of small grooves or small perforations provided to the upper part of a partition plate. CONSTITUTION:A first and a second partition plates 10a, 10b used as the partition plates for dividing a treating tank 1 into three sections respectively have linear grooves 11a, 11b provided to the upper parts thereof and the outer side walls 12a, 12b of the linear grooves 11a, 11b have a large number grooves 13 opposed to a first and a second water absorbing sheet 6a, 6b. Therefore, the buffer solutions after neutrailization treatment flowed into the linear grooves 11a, 11b from pipes 14a, 14b are fallen on the upper regions of the water absorbing sheets 6a, 6b through a large number of the small grooves 13. As a result, the alomst all regions of the water absorbing sheets 6a, 6b are washed by the buffer solutions after neutralization treatment and plural times of electrophoretic treatments can be achieved well without replacing the absorbing sheet.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to an electrophoresis apparatus particularly suitable for creating electrophoresis patterns of serum proteins.

Conventional Structure and Problems It is common practice to create electrophoretic patterns of serum proteins for the purpose of human health management.

In this case, as shown in FIG. 1, a serum sample 2 is coated on a sheet-like support 1 made of a cellulose acetate membrane or the like and subjected to electrophoresis treatment.
The serum sample 2 above is developed to a length of 15 to 50 mm, creating a striped latent image depending on the protein content of the serum sample.

This latent image becomes a colored striped pattern as shown in Figure 2 by the selective staining process that is performed next, and by analyzing the shading of this striped pattern 3, the quality of the serum sample is determined. .

By the way, an electrophoresis apparatus is configured as shown in FIG. 3, for example. The internal space of the treatment tank 4 is watertightly partitioned by first and second partition plates 5a and 5b, and the first water-absorbing sheet 6a hanging down from the top of the first partition plate 5a is
The second water-absorbing sheet 6b that sucks up the buffer solution 8a in the liquid reservoir 7a and hangs down from the top of the second partition plate 5b is used as the second liquid reservoir 7b.
The buffer solution 8b inside is sucked up. For this reason, the first partition plate 5a
The sheet-like support 1 placed on the first and second water-absorbing sheets 6a and eb so as to be separated by the top of the second partition plate 5b and the top of the second partition plate 5b contains a buffer solution and is kept in a wet state. . 9a
, 9b indicate first and second electrodes made of platinum wire provided in the first and twentieth liquid reservoirs 7a and 7b.

To create an electrophoretic pattern of serum proteins using the apparatus configured as described above, first, the serum sample 2 is applied dropwise onto the surface of the sorted support 10, and then the first and second electrodes ga , gb are used as both electrodes, and an appropriate DC voltage is applied for a predetermined period of time. Since the latent image is thus obtained, the sheet-like support 1 taken out from the processing tank 4 is subjected to selective dyeing treatment, while a new sheet-like support is installed in the processing tank 4 and the next step is carried out. Perform electrophoresis treatment.

By the way, the buffer solutions 8a and 8b remaining in the first and second liquid reservoirs 7a and 7b are removed during the previous electrophoresis process.
Since there are differences in pH ratio and ionic strength due to negative polarity, it is necessary to equalize these differences. Therefore,
A neutralization method has been adopted in which the buffer solutions aa and sb are once collected by a pump etc., mixed and distributed to the reservoirs 7a and 7b. It was not possible to replace almost the entire amount of the buffer solution with the buffer solution after the neutralization treatment. However, if multiple electrophoresis treatments are performed without replacing the first and second water absorption sheets 6a and 6b, the treatment effect will lack uniformity, which will seriously impede the intended purpose of the test. Become.

OBJECTS OF THE INVENTION Accordingly, an object of the present invention is to provide an electrophoresis device that can replace the buffer solution adsorbed on a water-absorbing sheet with a neutralized buffer solution.

Structure of the Invention In the electrophoresis apparatus of the present invention, when returning the recovered and neutralized buffer solution to the processing tank, the buffer solution is caused to flow out through a large number of small grooves or small holes provided in the upper part of the partition plate. The liquid is configured to flow into the reservoir along the water-absorbing sheet, and this will be described in detail below with reference to embodiments shown in the drawings.

DESCRIPTION OF THE EMBODIMENTS As shown in FIGS. 4 and 5, first and second partition plates 10a and 10 serve as partition plates that divide the inside of the processing tank 4 into three parts.
b has grooves 11L on the top of each.

11b, each groove 11&, an outer wall 12& of 11b.

12b has a large number of small grooves 13 facing the first or second water absorbing sheet. And each groove 10a, 10
A tube is inserted into each end of b.

1t&, 14b are mutual communication tubes 1sa, 1s
b.

The distal end edge of the canopy 18 is inserted into a collection tank 19, and is connected to the drain port of the first pump 1A through the pipe 16, and to the water intake port of the first pump 17.

In addition, there are tubes inserted into each of the liquid reservoirs 7a and 7b.

20a and 20b are for mutual communication/tube 21J 21b
.

The distal end edge of the tube 24, which communicates with the water intake port of the second pump 23 through 22 and the drain port of the second pump 23, is inserted into the recovery tank 19.

When the second pump 23 is rotated in the electrophoresis apparatus configured in this way, the first and second pumps, the reservoir 72
L, buffer in Tb 20J 20b.

21 &, 21 b, 22. It is collected into the collection tank 19 through the second pump 23 and the nozzle 24, and neutralized by mixing. Further, when the first pump 17 is rotated, the buffer solution 75 in the recovery tank 19 and the pipe 18 . The first and second reservoirs 7?L are supplied through the first pump 17 and the tube 16゜15&, 15b.

7b will be distributed and paid. And ) Kubu 14 &.

The neutralized buffer flowing into the grooves 11a and 11b from the grooves 14b absorbs water through a large number of small grooves 13 (6a),
It will fall on the upper region of 6b.

Because of this, almost the entire area of the water absorption sheets 6a and 6b is washed by the neutralized buffer flowing through them, not only renewing the buffer adsorbed on the sheets but also preventing clogging of the sheets. , it becomes possible to successfully perform multiple electrophoresis treatments without replacing the water-absorbing sheet.

In the embodiment shown in FIG. 6, a through hole 25 is provided in the upper part of the partition plate instead of the groove, and a large number of small holes 2 are connected to the through hole 25.
6 is provided on the outside wall. In this case, the neutralized buffer solution that has been injected into the through hole 25 through a pipe (not shown) is sprayed out in the form of a mist through the small hole 26 and hits the surface of the water absorption sheet 6b with force. Therefore, it is possible to provide the water-absorbing sheet with a better liquid renewal effect and a better effect of preventing tearing.

Effects of the Invention Since the electrophoresis device of the present invention operates as described above, not only the buffer solution in the first and second liquid reservoirs but also the buffer solution contained in the water absorption sheet can be updated at the same time. Since clogging of the water-absorbing sheet can also be prevented, multiple electrophoresis treatments can be performed successfully without replacing the water-absorbing sheet.

[Brief explanation of the drawing]

Figure 1 is a plan view showing the relationship between a water-absorbing sheet and a serum sample applied to its surface, Figure 2 is a plan view showing the electrophoresis pattern of serum proteins, and Figure 3 is a side cross-section of a conventional electrophoresis device. 4 is a side sectional view of an electrophoresis device embodying the present invention, FIG. 5 is a perspective view of the main parts of the same device, and FIG. 6 is a perspective view of the main parts of another embodiment of the invention. be. 1 sheet-like support, 6a, 6b water-absorbing sheet,
7a, 7b--Liquid reservoir, 10a, 10b partition plate, 11a, 1
1t) - groove, 13 small groove, 17.23 pump, 19 tank, 25 through hole, 26 small hole.

Claims (1)

[Claims] A first water-absorbing sheet that hangs down from the top of the first partition plate and sucks up the buffer solution in the first liquid reservoir, and a second water-absorbent sheet that hangs down from the top of the second partition plate and sucks up the buffer solution in the second liquid reservoir. In an electrophoresis apparatus in which a buffer solution is contained in a sheet-like support for supporting a specimen, a large number of small grooves or small holes are provided on each upper part of the first and second partition plates facing the first or second water-absorbing sort. An electrophoresis apparatus characterized in that a groove or a through hole communicating with the small groove or small hole is communicated with a drain port of a pump leading to a liquid recovery tank.