JPS586480B2 - 酸性ホスフアタ−ゼの定量 - Google Patents
酸性ホスフアタ−ゼの定量Info
- Publication number
- JPS586480B2 JPS586480B2 JP15352676A JP15352676A JPS586480B2 JP S586480 B2 JPS586480 B2 JP S586480B2 JP 15352676 A JP15352676 A JP 15352676A JP 15352676 A JP15352676 A JP 15352676A JP S586480 B2 JPS586480 B2 JP S586480B2
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- phosphomonoester
- phosphate
- buffer
- pentanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000013563 Acid Phosphatase Human genes 0.000 title claims description 20
- 108010051457 Acid Phosphatase Proteins 0.000 title claims description 20
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 title claims description 19
- 238000011002 quantification Methods 0.000 title description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 47
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 34
- 239000000758 substrate Substances 0.000 claims description 31
- 238000006243 chemical reaction Methods 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000000872 buffer Substances 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 14
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 12
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 12
- 239000012190 activator Substances 0.000 claims description 12
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 11
- 125000004432 carbon atom Chemical group C* 0.000 claims description 10
- JYVLIDXNZAXMDK-UHFFFAOYSA-N pentan-2-ol Chemical compound CCCC(C)O JYVLIDXNZAXMDK-UHFFFAOYSA-N 0.000 claims description 10
- 229910019142 PO4 Inorganic materials 0.000 claims description 9
- 210000001124 body fluid Anatomy 0.000 claims description 9
- 239000010839 body fluid Substances 0.000 claims description 9
- 125000000962 organic group Chemical group 0.000 claims description 9
- 239000010452 phosphate Substances 0.000 claims description 9
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 claims description 8
- 239000012928 buffer substance Substances 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 8
- 239000008351 acetate buffer Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- LDKDGDIWEUUXSH-UHFFFAOYSA-N Thymophthalein Chemical compound C1=C(O)C(C(C)C)=CC(C2(C3=CC=CC=C3C(=O)O2)C=2C(=CC(O)=C(C(C)C)C=2)C)=C1C LDKDGDIWEUUXSH-UHFFFAOYSA-N 0.000 claims description 6
- 239000007979 citrate buffer Substances 0.000 claims description 6
- 238000006911 enzymatic reaction Methods 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- ALQSHHUCVQOPAS-UHFFFAOYSA-N Pentane-1,5-diol Chemical compound OCCCCCO ALQSHHUCVQOPAS-UHFFFAOYSA-N 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- CMPQUABWPXYYSH-UHFFFAOYSA-N phenyl phosphate Chemical compound OP(O)(=O)OC1=CC=CC=C1 CMPQUABWPXYYSH-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 239000003599 detergent Substances 0.000 claims description 4
- -1 naphthyl phosphate Chemical compound 0.000 claims description 4
- 239000003381 stabilizer Substances 0.000 claims description 4
- WMDDNKROYKCDJC-UHFFFAOYSA-N [4-[3-oxo-1-(4-phosphonooxyphenyl)-2-benzofuran-1-yl]phenyl] dihydrogen phosphate Chemical compound C1=CC(OP(O)(=O)O)=CC=C1C1(C=2C=CC(OP(O)(O)=O)=CC=2)C2=CC=CC=C2C(=O)O1 WMDDNKROYKCDJC-UHFFFAOYSA-N 0.000 claims description 3
- DHCLVCXQIBBOPH-UHFFFAOYSA-N beta-glycerol phosphate Natural products OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 claims description 3
- GHRQXJHBXKYCLZ-UHFFFAOYSA-L beta-glycerolphosphate Chemical compound [Na+].[Na+].CC(CO)OOP([O-])([O-])=O GHRQXJHBXKYCLZ-UHFFFAOYSA-L 0.000 claims description 3
- 210000003743 erythrocyte Anatomy 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- IKQSNKKSCOCCOD-UHFFFAOYSA-N molport-028-600-099 Chemical group OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1Cl IKQSNKKSCOCCOD-UHFFFAOYSA-N 0.000 claims description 2
- 150000004712 monophosphates Chemical class 0.000 claims description 2
- 239000012086 standard solution Substances 0.000 claims description 2
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 claims 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims 1
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 claims 1
- 238000005375 photometry Methods 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 239000000654 additive Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 4
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 3
- BTJIUGUIPKRLHP-UHFFFAOYSA-M 4-nitrophenolate Chemical compound [O-]C1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-M 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 210000002307 prostate Anatomy 0.000 description 3
- SGPKEYSZPHMVNI-UHFFFAOYSA-N 2-bromo-1-(2-hydroxyphenyl)ethanone Chemical compound OC1=CC=CC=C1C(=O)CBr SGPKEYSZPHMVNI-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000001045 blue dye Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- ANAZDOWEOOFEET-UHFFFAOYSA-N (4-hydroxyphenyl) [4-(3-oxo-1h-2-benzofuran-1-yl)phenyl] hydrogen phosphate Chemical compound C1=CC(O)=CC=C1OP(O)(=O)OC1=CC=C(C2C3=CC=CC=C3C(=O)O2)C=C1 ANAZDOWEOOFEET-UHFFFAOYSA-N 0.000 description 1
- 125000001637 1-naphthyl group Chemical group [H]C1=C([H])C([H])=C2C(*)=C([H])C([H])=C([H])C2=C1[H] 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- XYZZKVRWGOWVGO-UHFFFAOYSA-N Glycerol-phosphate Chemical compound OP(O)(O)=O.OCC(O)CO XYZZKVRWGOWVGO-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 229910018828 PO3H2 Inorganic materials 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 125000006501 nitrophenyl group Chemical group 0.000 description 1
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 229920000259 polyoxyethylene lauryl ether Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000006462 rearrangement reaction Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 1
- 235000019801 trisodium phosphate Nutrition 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1677975A CH625833A5 (en) | 1975-12-24 | 1975-12-24 | Method for the determination of acid phosphatase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5282386A JPS5282386A (en) | 1977-07-09 |
| JPS586480B2 true JPS586480B2 (ja) | 1983-02-04 |
Family
ID=4420153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15352676A Expired JPS586480B2 (ja) | 1975-12-24 | 1976-12-22 | 酸性ホスフアタ−ゼの定量 |
Country Status (11)
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3425912A (en) * | 1966-11-14 | 1969-02-04 | Smithkline Corp | Laboratory reagent for assay of alkaline phosphatase |
| GB1263202A (en) * | 1970-01-14 | 1972-02-09 | Pfizer | Alkaline phosphatase test |
| US3823071A (en) * | 1970-12-21 | 1974-07-09 | Worthington Biochem Corp | Prostatic acid phosphatase determination |
| DE2115748C3 (de) * | 1971-03-31 | 1981-07-16 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Bestimmung der sauren Phosphatase |
-
1975
- 1975-12-24 CH CH1677975A patent/CH625833A5/de not_active IP Right Cessation
-
1976
- 1976-11-30 IT IT2993976A patent/IT1154005B/it active
- 1976-12-02 CA CA267,027A patent/CA1070597A/en not_active Expired
- 1976-12-10 NL NL7613768A patent/NL178798C/xx not_active IP Right Cessation
- 1976-12-22 JP JP15352676A patent/JPS586480B2/ja not_active Expired
- 1976-12-22 DD DD19654676A patent/DD128970A5/xx unknown
- 1976-12-23 GB GB5371076A patent/GB1563517A/en not_active Expired
- 1976-12-23 SE SE7614532A patent/SE425098B/xx not_active IP Right Cessation
- 1976-12-23 DE DE19762658634 patent/DE2658634C2/de not_active Expired
- 1976-12-23 FR FR7638870A patent/FR2336683A1/fr active Granted
- 1976-12-23 DK DK582776A patent/DK582776A/da unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK582776A (da) | 1977-06-25 |
| GB1563517A (en) | 1980-03-26 |
| DE2658634A1 (de) | 1977-07-07 |
| JPS5282386A (en) | 1977-07-09 |
| NL178798B (nl) | 1985-12-16 |
| DE2658634C2 (de) | 1984-03-01 |
| FR2336683A1 (fr) | 1977-07-22 |
| CH625833A5 (en) | 1981-10-15 |
| IT1154005B (it) | 1987-01-21 |
| CA1070597A (en) | 1980-01-29 |
| NL178798C (nl) | 1986-05-16 |
| SE425098B (sv) | 1982-08-30 |
| DD128970A5 (de) | 1977-12-21 |
| NL7613768A (nl) | 1977-06-28 |
| FR2336683B1 (enrdf_load_stackoverflow) | 1980-10-03 |
| SE7614532L (sv) | 1977-06-25 |
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