JPS5792000A - Purifying and separating method of vitamin b12 - Google Patents

Purifying and separating method of vitamin b12

Info

Publication number
JPS5792000A
JPS5792000A JP16737480A JP16737480A JPS5792000A JP S5792000 A JPS5792000 A JP S5792000A JP 16737480 A JP16737480 A JP 16737480A JP 16737480 A JP16737480 A JP 16737480A JP S5792000 A JPS5792000 A JP S5792000A
Authority
JP
Japan
Prior art keywords
vitamin
divinylbenzene
resin
separate
adsorbed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP16737480A
Other languages
Japanese (ja)
Other versions
JPS6324519B2 (en
Inventor
Tatsuo Kinoshita
Ichiro Kojima
Kenji Maruhashi
Yutaka Oguchi
Noboru Endo
Tetsuo Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eneos Corp
Original Assignee
Nippon Oil Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Oil Corp filed Critical Nippon Oil Corp
Priority to JP16737480A priority Critical patent/JPS5792000A/en
Priority to US06/324,633 priority patent/US4383110A/en
Priority to GB8135647A priority patent/GB2088383B/en
Priority to CH762381A priority patent/CH646716A5/en
Priority to DE19813147130 priority patent/DE3147130A1/en
Priority to FR8122356A priority patent/FR2495161A1/en
Publication of JPS5792000A publication Critical patent/JPS5792000A/en
Publication of JPS6324519B2 publication Critical patent/JPS6324519B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE: To purify and separate vitamin B12 industrially and advantageously without requiring an additional operation, by adsorbing a solution containing impure vitamin B12 on a divinylbenzene/styrene copolymeric resin having a sepecific pore diameter and pore volume as an adsorbent, and eluting the adsorbed vitamin B12.
CONSTITUTION: A solution, containing vitamin B12 and impurities, and obtained by the fermentation, cellular extraction methods, etc. is brought into contact with a divinylbenzene/styrene copolymeric resin having the most frequent cellular pore diameter ≥200Å, preferably 250W1,200Å, and a pore volume >0.6ml/g, preferably 0.68W1.2ml/g, by the column chromatographic method, etc. to adsorb the vitamin B12 in the resin. The adsorbed vitamin B12 is then eluted with an eluting agent, e.g. 20% methanol-water, to elute and separate the vitamin B12 component.
COPYRIGHT: (C)1982,JPO&Japio
JP16737480A 1980-11-29 1980-11-29 Purifying and separating method of vitamin b12 Granted JPS5792000A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP16737480A JPS5792000A (en) 1980-11-29 1980-11-29 Purifying and separating method of vitamin b12
US06/324,633 US4383110A (en) 1980-11-29 1981-11-24 Process for purifying and separating vitamin B12
GB8135647A GB2088383B (en) 1980-11-29 1981-11-26 Process for purifying and separating vitamin b12
CH762381A CH646716A5 (en) 1980-11-29 1981-11-27 METHOD FOR PRODUCING VITAMIN B12 IN PURE FORM.
DE19813147130 DE3147130A1 (en) 1980-11-29 1981-11-27 METHOD FOR CLEANING AND SEPARATING VITAMIN B12
FR8122356A FR2495161A1 (en) 1980-11-29 1981-11-30 PROCESS FOR PURIFYING AND SEPARATING B12 VITAMINS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16737480A JPS5792000A (en) 1980-11-29 1980-11-29 Purifying and separating method of vitamin b12

Publications (2)

Publication Number Publication Date
JPS5792000A true JPS5792000A (en) 1982-06-08
JPS6324519B2 JPS6324519B2 (en) 1988-05-20

Family

ID=15848523

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16737480A Granted JPS5792000A (en) 1980-11-29 1980-11-29 Purifying and separating method of vitamin b12

Country Status (1)

Country Link
JP (1) JPS5792000A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59220198A (en) * 1983-02-11 1984-12-11 リヒタ−　ゲデオン　ベジエセテイ　ジヤ−ル　ア−ル．テ−． Treatment of fermentation liquid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59220198A (en) * 1983-02-11 1984-12-11 リヒタ−　ゲデオン　ベジエセテイ　ジヤ−ル　ア−ル．テ−． Treatment of fermentation liquid

Also Published As

Publication number Publication date
JPS6324519B2 (en) 1988-05-20

Similar Documents

Publication Publication Date Title
DK0658164T3 (en) Process for chromatographic purification and separation of nucleic acid mixtures
CA2077176A1 (en) Gas separation process and unit therefor
CA2275357A1 (en) Method for separating paraxylene comprising adsorption with water injection and crystallisation
MX9707909A (en) Antibody purification by low-ph hydrophobic interaction chromatoggraphy.
MY104064A (en) An integrated process for the removal of sulfur compounds from fluid streams
MY101885A (en) A process for separating and purifying metallic elements by displacement chromatography
ES8103003A1 (en) Two-stage process for separating mixed fatty-acid esters
JPS5444199A (en) Separation system of isotopes
AU6563300A (en) Process for separating essential oils from an essential oil-containing material
JPS5792000A (en) Purifying and separating method of vitamin b12
Reeve et al. Purification of plant hormone extracts by gel permeation chromatography
JPS5441899A (en) Purification of stevioside
FR2449671B1 (en) PROCESS FOR SEPARATING ORTHOCHLORONITROBENZENE
JPS5231038A (en) Separation process of p-xylene by adsorption
JPS56113725A (en) Separation of aliphatic aldehyde isomer mixture
YU46194A (en) PROCEDURE FOR DIVISION OF LIQUID MIXTURE COMPONENTS
Hill et al. Frontal chromatographic techniques in preparative chromatography
JPS52152893A (en) Separation and recovery of nitrogen
JPS5663804A (en) Oxygen concentrating method
JPS57207507A (en) Separation by adsorption
JPS56145299A (en) Purification of s-adenosyl-l-methionine
JPS55167228A (en) Purification of high-purity kallikrein
JPS5557530A (en) Separation of phenol isomer substituted with polyhydrocarbon
JPS57206626A (en) Separating method of isomer
JPS5373526A (en) Adsorptive separation of xylene isomers