JPS5786288A - Anaerobic cultivating method - Google Patents
Anaerobic cultivating methodInfo
- Publication number
- JPS5786288A JPS5786288A JP16178380A JP16178380A JPS5786288A JP S5786288 A JPS5786288 A JP S5786288A JP 16178380 A JP16178380 A JP 16178380A JP 16178380 A JP16178380 A JP 16178380A JP S5786288 A JPS5786288 A JP S5786288A
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- container
- sealed
- oxygen
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To carry out the anaerobic cultivation of microorganisms easily, by the presence of an oxygen absorbing agent in a chamber separated from a culture medium through a thin film having the water impermeability and oxygen permeability.
CONSTITUTION: A thin film, e.g. polypropylene or polyethylene, is used as a material having the water impermeability and oxygen permeability. A culture medium is sealed in a container consisting of the material, sterilized and inoculated with a microorganism. The inoculated culture medium is then introduced into a large-sized container containing an aqueous solution of a sulfite as an oxygen absorbing agent and cultivated. The cultivation may be carried out by the following other methods: (1) The culture medium is introduced into a general sealed container for the cultivation, and a container, molded from the material, and containing the oxygen absorbing agent is introduced into the culture medium. (2) One of the materials capable of absorbing oxygen on mixing, e.g. ferrous sulfate or pyrogallol and an aqueous solution of sodium hydroxide, is sealed in an ampul, sealed with the other in the container, and then mixed therewith on breaking the ampul in use.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16178380A JPS5823074B2 (en) | 1980-11-17 | 1980-11-17 | Anaerobic culture method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16178380A JPS5823074B2 (en) | 1980-11-17 | 1980-11-17 | Anaerobic culture method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5786288A true JPS5786288A (en) | 1982-05-29 |
JPS5823074B2 JPS5823074B2 (en) | 1983-05-12 |
Family
ID=15741817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16178380A Expired JPS5823074B2 (en) | 1980-11-17 | 1980-11-17 | Anaerobic culture method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5823074B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0502076A1 (en) * | 1989-11-27 | 1992-09-09 | Martek Corporation | Closed photobioreactor |
-
1980
- 1980-11-17 JP JP16178380A patent/JPS5823074B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0502076A1 (en) * | 1989-11-27 | 1992-09-09 | Martek Corporation | Closed photobioreactor |
Also Published As
Publication number | Publication date |
---|---|
JPS5823074B2 (en) | 1983-05-12 |
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