JPS5764160A - Method and apparatus for measuring urea - Google Patents

Method and apparatus for measuring urea

Info

Publication number
JPS5764160A
JPS5764160A JP55139606A JP13960680A JPS5764160A JP S5764160 A JPS5764160 A JP S5764160A JP 55139606 A JP55139606 A JP 55139606A JP 13960680 A JP13960680 A JP 13960680A JP S5764160 A JPS5764160 A JP S5764160A
Authority
JP
Japan
Prior art keywords
solution
bro
concentration
measured
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55139606A
Other languages
Japanese (ja)
Other versions
JPS6317181B2 (en
Inventor
Hisayuki Ikeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yokogawa Electric Corp
Original Assignee
Yokogawa Electric Corp
Yokogawa Hokushin Electric Corp
Yokogawa Electric Works Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yokogawa Electric Corp, Yokogawa Hokushin Electric Corp, Yokogawa Electric Works Ltd filed Critical Yokogawa Electric Corp
Priority to JP55139606A priority Critical patent/JPS5764160A/en
Publication of JPS5764160A publication Critical patent/JPS5764160A/en
Publication of JPS6317181B2 publication Critical patent/JPS6317181B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To accrately measure urea concentration by acting urease-enzyme to the urea-contg. solution to be measured and allowing the resulting NH3 to contact and react with the solution contg. BrO<->, then detecting the change of BrO<-> concentration by polarography. CONSTITUTION:A prescribed amount of the solution to be measured is introduced from an inlet opening 1a of a sampling valve 1, and reacted with urease-enzyme 2 to produce NH3. After that, the reaction product is introduced to a flow cell 4 to transmit NH3 alone through a high polymer membrane 4c. On the other hand, an electrolytic solution contg. Br<-> is supplied from a container 8 for electrolytic solution to a reagent producing apparatus 7, and there BrO<-> is produced by electrolytic oxidation. Then it is introduced to the secondary chamber 4b to be reacted with said NH3. Consequently, the change of BrO<-> concentration is detected by polarography through electrodes 4d, 4e, 4f which are charged with onstant voltage from a potentiostat 5, and after prescribed signal treatment, the urea concentration in the solution to be measured in indicated in an indicater 6.
JP55139606A 1980-10-06 1980-10-06 Method and apparatus for measuring urea Granted JPS5764160A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55139606A JPS5764160A (en) 1980-10-06 1980-10-06 Method and apparatus for measuring urea

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55139606A JPS5764160A (en) 1980-10-06 1980-10-06 Method and apparatus for measuring urea

Publications (2)

Publication Number Publication Date
JPS5764160A true JPS5764160A (en) 1982-04-19
JPS6317181B2 JPS6317181B2 (en) 1988-04-12

Family

ID=15249192

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55139606A Granted JPS5764160A (en) 1980-10-06 1980-10-06 Method and apparatus for measuring urea

Country Status (1)

Country Link
JP (1) JPS5764160A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2435510A (en) * 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
US7935497B2 (en) 2006-02-23 2011-05-03 Mologic Ltd Protease detection
US8241588B2 (en) 2006-02-23 2012-08-14 Mologic Ltd Binding assay

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2435510A (en) * 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
US7935497B2 (en) 2006-02-23 2011-05-03 Mologic Ltd Protease detection
US8241588B2 (en) 2006-02-23 2012-08-14 Mologic Ltd Binding assay
US8361386B2 (en) 2006-02-23 2013-01-29 Mologic Ltd Enzyme detection
US8846328B2 (en) 2006-02-23 2014-09-30 Mologic Ltd Method for detecting the presence of a protease enzyme in a sample

Also Published As

Publication number Publication date
JPS6317181B2 (en) 1988-04-12

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