JPS5759157A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS5759157A
JPS5759157A JP55134907A JP13490780A JPS5759157A JP S5759157 A JPS5759157 A JP S5759157A JP 55134907 A JP55134907 A JP 55134907A JP 13490780 A JP13490780 A JP 13490780A JP S5759157 A JPS5759157 A JP S5759157A
Authority
JP
Japan
Prior art keywords
coenzyme
substrate
immobilizing
electrode
adsorbing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55134907A
Other languages
Japanese (ja)
Other versions
JPS6243496B2 (en
Inventor
Shiro Nankai
Kenichi Nakamura
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP55134907A priority Critical patent/JPS5759157A/en
Publication of JPS5759157A publication Critical patent/JPS5759157A/en
Publication of JPS6243496B2 publication Critical patent/JPS6243496B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To obtain an enzyme electrode responding quickly to a change in the concn. of substrate by adsorbing and immobilizing coenzyme on a conductive carrier, and immobilizing oxidoreductase to the resultant carrier by a cross-linking method. CONSTITUTION:A material which is conductive, has good adsorbing and holding power for coenzyme and is chemically stable, such as acetylene black, is molded under pressure as a binder, whereby an electrode substrate 1 is made. An aq. soln. of, for example, coenzyme nicotinamide adenine nucleotide (NAD) is developed on the substrate 1, after which it is dried, whereby an adsorbing and immobilizing layer 2 for coenzyme is provided. Next, an aq. soln. of oxidoreductase alcohol dehydrogenase is developed on the layer 2 and is dried, whereby an immobilizing layer 3 is formed. Thence these are caused to crosslink and react in the vapor of glutaraldehyde, whereby the enzyme electrode is obtained. This enzyme electrode is mounted in the bottom end part of a holder 7, and is immersed in buffer 8 contg. substrate (ethanol) 8, so that the analysis of good response speed is accomplished by using a device constituting a counter electrode 10, a salt bridge 9, a reference electrode 9, a potentiostat 5, and a recorder 4.
JP55134907A 1980-09-27 1980-09-27 Enzyme electrode Granted JPS5759157A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55134907A JPS5759157A (en) 1980-09-27 1980-09-27 Enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55134907A JPS5759157A (en) 1980-09-27 1980-09-27 Enzyme electrode

Publications (2)

Publication Number Publication Date
JPS5759157A true JPS5759157A (en) 1982-04-09
JPS6243496B2 JPS6243496B2 (en) 1987-09-14

Family

ID=15139310

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55134907A Granted JPS5759157A (en) 1980-09-27 1980-09-27 Enzyme electrode

Country Status (1)

Country Link
JP (1) JPS5759157A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02209300A (en) * 1989-02-10 1990-08-20 Takara Co Ltd Decorative body

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54140594A (en) * 1978-04-24 1979-10-31 Matsushita Electric Ind Co Ltd Enzyme electrode

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54140594A (en) * 1978-04-24 1979-10-31 Matsushita Electric Ind Co Ltd Enzyme electrode

Also Published As

Publication number Publication date
JPS6243496B2 (en) 1987-09-14

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