JPS54140594A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS54140594A JPS54140594A JP4887978A JP4887978A JPS54140594A JP S54140594 A JPS54140594 A JP S54140594A JP 4887978 A JP4887978 A JP 4887978A JP 4887978 A JP4887978 A JP 4887978A JP S54140594 A JPS54140594 A JP S54140594A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- nad
- disk
- auxiliary
- oxidized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To easily quantitize the (CH3)3N, which is generated in accordance with the putrefaction of fish meat, by fixing special oxidizing and reducing enzyme or an auxiliary enzyme on to in the vicinity of an electric collector, respectively.
CONSTITUTION: As a trimethyl amine oxide reducing enzyme and an auxiliary enzyme, oxidized type nicotinamide adenine dinucleotide NAD is dissolved in a phosphoric acid of pH 7.0. The solution thus prepared is applied to a disk, which is press-molded of graphite powders, and driyed so that it is acted with flutaraldehyde. The graphite disk 4, on the surface of which the enzyme and auxiliary enzyme are bridged, is fixed to a substrate 1 by a screwing member 5, which is made of an insulating material, such that it is in contact with a Pt plate 3 having a lead wire 2 connected therewith. In case there exists (SH3)3N, the catalyzer reaction of the enzymes in the opposite direction to that shown so that the NADH made of NAD+ is oxidized electro-chemically on the disk 4 by the NAD+ and so that the concentration of the (CH3)N can be obtained from the value of the oxidizing current.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53048879A JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53048879A JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54140594A true JPS54140594A (en) | 1979-10-31 |
JPS5816695B2 JPS5816695B2 (en) | 1983-04-01 |
Family
ID=12815565
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53048879A Expired JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5816695B2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5759157A (en) * | 1980-09-27 | 1982-04-09 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
US4968400A (en) * | 1986-11-20 | 1990-11-06 | Terumo Kabushiki Kaisha | Enzyme sensor |
US5122456A (en) * | 1987-05-01 | 1992-06-16 | Cambridge Life Science Plc | Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution |
-
1978
- 1978-04-24 JP JP53048879A patent/JPS5816695B2/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5759157A (en) * | 1980-09-27 | 1982-04-09 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
JPS6243496B2 (en) * | 1980-09-27 | 1987-09-14 | Matsushita Electric Ind Co Ltd | |
US4968400A (en) * | 1986-11-20 | 1990-11-06 | Terumo Kabushiki Kaisha | Enzyme sensor |
US5122456A (en) * | 1987-05-01 | 1992-06-16 | Cambridge Life Science Plc | Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution |
Also Published As
Publication number | Publication date |
---|---|
JPS5816695B2 (en) | 1983-04-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0241309A3 (en) | Measurement of electroactive species in solution | |
HUT66200A (en) | Sensor for measuring the quantity of a dissolved component and apparatus comprising it | |
MY101817A (en) | An additive composition for increasing the conductivity of an organic polymer, an active hydrogen-containing composition containing the additive composition, and an organic polymer composition containing the additive composition | |
CA2305800A1 (en) | Biosensor electrode mediators for regeneration of cofactors | |
ES8302122A1 (en) | Improved anode with lead base and method of making same. | |
DE3466323D1 (en) | Platinum-iron electrocatalyst and fuel cell electrode using the same | |
JPS55500889A (en) | ||
UA42670C2 (en) | Method for electric power generation of organic matters | |
JPS5510581A (en) | Enzyem electrode | |
NO164487C (en) | ELECTRODE FOR ELECTROCHEMICAL CELLS, LOW HYDROGEN OVERVOLTING CATHODE AND PROCEDURE FOR THE PREPARATION OF SUCH A CATODO. | |
JPS6460963A (en) | Lead storage battery | |
DE69016385D1 (en) | Solid state sensor for determining the concentration of hydrogen-reactive gases. | |
EP0165024A3 (en) | Cathode alloy electrocatalysts | |
JPS54140594A (en) | Enzyme electrode | |
Tanaka et al. | Mediating effects of ferric chelate compounds in microbial fuel cells | |
ES2010036A6 (en) | Electrochemical cells. | |
ES8106337A1 (en) | Material for electrodes and electrolytic cells with anodes consisting of this material. | |
JPS5512437A (en) | Enzyme electrode | |
JPS5513860A (en) | Enzyme electrode | |
JPS57177699A (en) | Determination of alpha-amylase by means of enzyme electrode method | |
FR2356286A1 (en) | Electrochemical compsn. esp. for fuel cell cathodes - consists of a mixed oxide of manganese and copper, nickel and/or silver | |
Bremer et al. | Phosphorylation coupled to acyl-coenzyme A dehydrogenase-linked oxidation of fatty acids by liver and heart mitochondria | |
Sepa et al. | Symmetry factor and transfer coefficient in analysis of enthalpies of activation and mechanisms of oxygen reduction at platinum electrodes | |
ATE27209T1 (en) | ELECTROCATALYST AND FUEL CELL ELECTRODE USING THESE ELECTRIC CATALYST. | |
CA2376549A1 (en) | Biological activity probe for the detection of sulphide and sulphide producing microorganisms |