JPS54140594A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS54140594A
JPS54140594A JP4887978A JP4887978A JPS54140594A JP S54140594 A JPS54140594 A JP S54140594A JP 4887978 A JP4887978 A JP 4887978A JP 4887978 A JP4887978 A JP 4887978A JP S54140594 A JPS54140594 A JP S54140594A
Authority
JP
Japan
Prior art keywords
enzyme
nad
disk
auxiliary
oxidized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4887978A
Other languages
Japanese (ja)
Other versions
JPS5816695B2 (en
Inventor
Kenichi Nakamura
Shiro Nankai
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP53048879A priority Critical patent/JPS5816695B2/en
Publication of JPS54140594A publication Critical patent/JPS54140594A/en
Publication of JPS5816695B2 publication Critical patent/JPS5816695B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To easily quantitize the (CH3)3N, which is generated in accordance with the putrefaction of fish meat, by fixing special oxidizing and reducing enzyme or an auxiliary enzyme on to in the vicinity of an electric collector, respectively.
CONSTITUTION: As a trimethyl amine oxide reducing enzyme and an auxiliary enzyme, oxidized type nicotinamide adenine dinucleotide NAD is dissolved in a phosphoric acid of pH 7.0. The solution thus prepared is applied to a disk, which is press-molded of graphite powders, and driyed so that it is acted with flutaraldehyde. The graphite disk 4, on the surface of which the enzyme and auxiliary enzyme are bridged, is fixed to a substrate 1 by a screwing member 5, which is made of an insulating material, such that it is in contact with a Pt plate 3 having a lead wire 2 connected therewith. In case there exists (SH3)3N, the catalyzer reaction of the enzymes in the opposite direction to that shown so that the NADH made of NAD+ is oxidized electro-chemically on the disk 4 by the NAD+ and so that the concentration of the (CH3)N can be obtained from the value of the oxidizing current.
COPYRIGHT: (C)1979,JPO&Japio
JP53048879A 1978-04-24 1978-04-24 enzyme electrode Expired JPS5816695B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53048879A JPS5816695B2 (en) 1978-04-24 1978-04-24 enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53048879A JPS5816695B2 (en) 1978-04-24 1978-04-24 enzyme electrode

Publications (2)

Publication Number Publication Date
JPS54140594A true JPS54140594A (en) 1979-10-31
JPS5816695B2 JPS5816695B2 (en) 1983-04-01

Family

ID=12815565

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53048879A Expired JPS5816695B2 (en) 1978-04-24 1978-04-24 enzyme electrode

Country Status (1)

Country Link
JP (1) JPS5816695B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5759157A (en) * 1980-09-27 1982-04-09 Matsushita Electric Ind Co Ltd Enzyme electrode
US4968400A (en) * 1986-11-20 1990-11-06 Terumo Kabushiki Kaisha Enzyme sensor
US5122456A (en) * 1987-05-01 1992-06-16 Cambridge Life Science Plc Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5759157A (en) * 1980-09-27 1982-04-09 Matsushita Electric Ind Co Ltd Enzyme electrode
JPS6243496B2 (en) * 1980-09-27 1987-09-14 Matsushita Electric Ind Co Ltd
US4968400A (en) * 1986-11-20 1990-11-06 Terumo Kabushiki Kaisha Enzyme sensor
US5122456A (en) * 1987-05-01 1992-06-16 Cambridge Life Science Plc Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution

Also Published As

Publication number Publication date
JPS5816695B2 (en) 1983-04-01

Similar Documents

Publication Publication Date Title
EP0241309A3 (en) Measurement of electroactive species in solution
HUT66200A (en) Sensor for measuring the quantity of a dissolved component and apparatus comprising it
MY101817A (en) An additive composition for increasing the conductivity of an organic polymer, an active hydrogen-containing composition containing the additive composition, and an organic polymer composition containing the additive composition
CA2305800A1 (en) Biosensor electrode mediators for regeneration of cofactors
ES8302122A1 (en) Improved anode with lead base and method of making same.
DE3466323D1 (en) Platinum-iron electrocatalyst and fuel cell electrode using the same
JPS55500889A (en)
UA42670C2 (en) Method for electric power generation of organic matters
JPS5510581A (en) Enzyem electrode
NO164487C (en) ELECTRODE FOR ELECTROCHEMICAL CELLS, LOW HYDROGEN OVERVOLTING CATHODE AND PROCEDURE FOR THE PREPARATION OF SUCH A CATODO.
JPS6460963A (en) Lead storage battery
DE69016385D1 (en) Solid state sensor for determining the concentration of hydrogen-reactive gases.
EP0165024A3 (en) Cathode alloy electrocatalysts
JPS54140594A (en) Enzyme electrode
Tanaka et al. Mediating effects of ferric chelate compounds in microbial fuel cells
ES2010036A6 (en) Electrochemical cells.
ES8106337A1 (en) Material for electrodes and electrolytic cells with anodes consisting of this material.
JPS5512437A (en) Enzyme electrode
JPS5513860A (en) Enzyme electrode
JPS57177699A (en) Determination of alpha-amylase by means of enzyme electrode method
FR2356286A1 (en) Electrochemical compsn. esp. for fuel cell cathodes - consists of a mixed oxide of manganese and copper, nickel and/or silver
Bremer et al. Phosphorylation coupled to acyl-coenzyme A dehydrogenase-linked oxidation of fatty acids by liver and heart mitochondria
Sepa et al. Symmetry factor and transfer coefficient in analysis of enthalpies of activation and mechanisms of oxygen reduction at platinum electrodes
ATE27209T1 (en) ELECTROCATALYST AND FUEL CELL ELECTRODE USING THESE ELECTRIC CATALYST.
CA2376549A1 (en) Biological activity probe for the detection of sulphide and sulphide producing microorganisms