JPS57131062A - Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier - Google Patents
Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrierInfo
- Publication number
- JPS57131062A JPS57131062A JP1501381A JP1501381A JPS57131062A JP S57131062 A JPS57131062 A JP S57131062A JP 1501381 A JP1501381 A JP 1501381A JP 1501381 A JP1501381 A JP 1501381A JP S57131062 A JPS57131062 A JP S57131062A
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- enzyme
- antibody
- labelled
- labelled antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 title abstract 11
- 102000036639 antigens Human genes 0.000 title abstract 11
- 108091007433 antigens Proteins 0.000 title abstract 11
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 108060003951 Immunoglobulin Proteins 0.000 abstract 2
- 239000012295 chemical reaction liquid Substances 0.000 abstract 2
- 102000018358 immunoglobulin Human genes 0.000 abstract 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- 229920002307 Dextran Polymers 0.000 abstract 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract 1
- 239000004698 Polyethylene Substances 0.000 abstract 1
- 102000005936 beta-Galactosidase Human genes 0.000 abstract 1
- 108010005774 beta-Galactosidase Proteins 0.000 abstract 1
- 230000001588 bifunctional effect Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- VPKDCDLSJZCGKE-UHFFFAOYSA-N methanediimine Chemical compound N=C=N VPKDCDLSJZCGKE-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- -1 polyethylene Polymers 0.000 abstract 1
- 229920000573 polyethylene Polymers 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1501381A JPS57131062A (en) | 1981-02-05 | 1981-02-05 | Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1501381A JPS57131062A (en) | 1981-02-05 | 1981-02-05 | Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57131062A true JPS57131062A (en) | 1982-08-13 |
| JPH0122905B2 JPH0122905B2 (enExample) | 1989-04-28 |
Family
ID=11876988
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1501381A Granted JPS57131062A (en) | 1981-02-05 | 1981-02-05 | Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57131062A (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6345561A (ja) * | 1986-07-24 | 1988-02-26 | マイルス・インコーポレーテッド | ポリアルキレングリコール架橋基を有する酵素標識抗体試薬 |
| US4980278A (en) * | 1985-05-28 | 1990-12-25 | Olympus Optical Co., Ltd. | Method of effecting immunological analysis and apparatus for carrying out the same |
| JPH08145995A (ja) * | 1994-11-24 | 1996-06-07 | B S R:Kk | Elisaによる血清中胆汁酸類の測定法および肝疾患診断法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49125517A (enExample) * | 1973-03-19 | 1974-12-02 | ||
| JPS51123819A (en) * | 1975-04-07 | 1976-10-28 | Summa Corp | Fixed immune adsorbing agent |
| US4104026A (en) * | 1976-03-12 | 1978-08-01 | University Of Virginia | Immunoassay separation technique |
| US4128628A (en) * | 1976-03-12 | 1978-12-05 | University Of Virginia Alumni Patents Foundation | Automated immunoassay |
| JPS5598359A (en) * | 1978-12-22 | 1980-07-26 | Amano Pharmaceut Co Ltd | Removing method of non-characteristic obstacle operation in immunity determination method |
| JPS55136261A (en) * | 1979-04-12 | 1980-10-23 | Toyo Jozo Co Ltd | Novel compound and kit comprising it |
-
1981
- 1981-02-05 JP JP1501381A patent/JPS57131062A/ja active Granted
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49125517A (enExample) * | 1973-03-19 | 1974-12-02 | ||
| JPS51123819A (en) * | 1975-04-07 | 1976-10-28 | Summa Corp | Fixed immune adsorbing agent |
| US4104026A (en) * | 1976-03-12 | 1978-08-01 | University Of Virginia | Immunoassay separation technique |
| US4128628A (en) * | 1976-03-12 | 1978-12-05 | University Of Virginia Alumni Patents Foundation | Automated immunoassay |
| JPS5598359A (en) * | 1978-12-22 | 1980-07-26 | Amano Pharmaceut Co Ltd | Removing method of non-characteristic obstacle operation in immunity determination method |
| JPS55136261A (en) * | 1979-04-12 | 1980-10-23 | Toyo Jozo Co Ltd | Novel compound and kit comprising it |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4980278A (en) * | 1985-05-28 | 1990-12-25 | Olympus Optical Co., Ltd. | Method of effecting immunological analysis and apparatus for carrying out the same |
| JPS6345561A (ja) * | 1986-07-24 | 1988-02-26 | マイルス・インコーポレーテッド | ポリアルキレングリコール架橋基を有する酵素標識抗体試薬 |
| JPH08145995A (ja) * | 1994-11-24 | 1996-06-07 | B S R:Kk | Elisaによる血清中胆汁酸類の測定法および肝疾患診断法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0122905B2 (enExample) | 1989-04-28 |
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