JPS57122756A - Purification of protein - Google Patents
Purification of proteinInfo
- Publication number
- JPS57122756A JPS57122756A JP56008593A JP859381A JPS57122756A JP S57122756 A JPS57122756 A JP S57122756A JP 56008593 A JP56008593 A JP 56008593A JP 859381 A JP859381 A JP 859381A JP S57122756 A JPS57122756 A JP S57122756A
- Authority
- JP
- Japan
- Prior art keywords
- ion
- liquid chromatography
- type counter
- phase
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To purify protein efficiently in high purity, by adding an anion type counter ion to a raw protein solution or mobile phase, carrying out reversed phase liquid chromatography.
CONSTITUTION: In purifying protein such as an enzyme contained in an animal organ, vegetable, fermentation or culture solution by liquid chromatography using a stationary phase of the reversed phase type, cyanopropylated silica gel is used as the stationary phase, an anion type counter ion such as an alkyl sulfonic acid ion, alkyl sulfuric acid ion, etc. is added to a raw protein solution or mobile phase to carry out liquid chromatography and to separate admixtures, and the anion type counter ion is released and removed compulosirly by electrodialysis or by ion exchange using an ion exchange resin.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56008593A JPS57122756A (en) | 1981-01-24 | 1981-01-24 | Purification of protein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56008593A JPS57122756A (en) | 1981-01-24 | 1981-01-24 | Purification of protein |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57122756A true JPS57122756A (en) | 1982-07-30 |
JPH0217154B2 JPH0217154B2 (en) | 1990-04-19 |
Family
ID=11697274
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56008593A Granted JPS57122756A (en) | 1981-01-24 | 1981-01-24 | Purification of protein |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57122756A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106872633A (en) * | 2017-04-11 | 2017-06-20 | 陕西慧康生物科技有限责任公司 | A kind of rp-hplc analysis method of recombinant human lysozyme |
-
1981
- 1981-01-24 JP JP56008593A patent/JPS57122756A/en active Granted
Non-Patent Citations (3)
Title |
---|
JOURNAL OF CHROMATOGRAPHY=1978 * |
JOURNAL OF CHROMATOGRAPHY=1979 * |
JOURNAL OF LIQUID CHROMATOGRAPHY=1978 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106872633A (en) * | 2017-04-11 | 2017-06-20 | 陕西慧康生物科技有限责任公司 | A kind of rp-hplc analysis method of recombinant human lysozyme |
Also Published As
Publication number | Publication date |
---|---|
JPH0217154B2 (en) | 1990-04-19 |
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