JPS57122756A - Purification of protein - Google Patents

Purification of protein

Info

Publication number
JPS57122756A
JPS57122756A JP56008593A JP859381A JPS57122756A JP S57122756 A JPS57122756 A JP S57122756A JP 56008593 A JP56008593 A JP 56008593A JP 859381 A JP859381 A JP 859381A JP S57122756 A JPS57122756 A JP S57122756A
Authority
JP
Japan
Prior art keywords
ion
liquid chromatography
type counter
phase
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56008593A
Other languages
Japanese (ja)
Other versions
JPH0217154B2 (en
Inventor
Naoki Asakawa
Akira Hashimoto
Yoshinobu Shinoda
Yasuo Miyake
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eisai Co Ltd
Original Assignee
Eisai Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai Co Ltd filed Critical Eisai Co Ltd
Priority to JP56008593A priority Critical patent/JPS57122756A/en
Publication of JPS57122756A publication Critical patent/JPS57122756A/en
Publication of JPH0217154B2 publication Critical patent/JPH0217154B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To purify protein efficiently in high purity, by adding an anion type counter ion to a raw protein solution or mobile phase, carrying out reversed phase liquid chromatography.
CONSTITUTION: In purifying protein such as an enzyme contained in an animal organ, vegetable, fermentation or culture solution by liquid chromatography using a stationary phase of the reversed phase type, cyanopropylated silica gel is used as the stationary phase, an anion type counter ion such as an alkyl sulfonic acid ion, alkyl sulfuric acid ion, etc. is added to a raw protein solution or mobile phase to carry out liquid chromatography and to separate admixtures, and the anion type counter ion is released and removed compulosirly by electrodialysis or by ion exchange using an ion exchange resin.
COPYRIGHT: (C)1982,JPO&Japio
JP56008593A 1981-01-24 1981-01-24 Purification of protein Granted JPS57122756A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56008593A JPS57122756A (en) 1981-01-24 1981-01-24 Purification of protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56008593A JPS57122756A (en) 1981-01-24 1981-01-24 Purification of protein

Publications (2)

Publication Number Publication Date
JPS57122756A true JPS57122756A (en) 1982-07-30
JPH0217154B2 JPH0217154B2 (en) 1990-04-19

Family

ID=11697274

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56008593A Granted JPS57122756A (en) 1981-01-24 1981-01-24 Purification of protein

Country Status (1)

Country Link
JP (1) JPS57122756A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106872633A (en) * 2017-04-11 2017-06-20 陕西慧康生物科技有限责任公司 A kind of rp-hplc analysis method of recombinant human lysozyme

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF CHROMATOGRAPHY=1978 *
JOURNAL OF CHROMATOGRAPHY=1979 *
JOURNAL OF LIQUID CHROMATOGRAPHY=1978 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106872633A (en) * 2017-04-11 2017-06-20 陕西慧康生物科技有限责任公司 A kind of rp-hplc analysis method of recombinant human lysozyme

Also Published As

Publication number Publication date
JPH0217154B2 (en) 1990-04-19

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