JPS5696699A - Determination of enzyme - Google Patents

Determination of enzyme

Info

Publication number
JPS5696699A
JPS5696699A JP17238179A JP17238179A JPS5696699A JP S5696699 A JPS5696699 A JP S5696699A JP 17238179 A JP17238179 A JP 17238179A JP 17238179 A JP17238179 A JP 17238179A JP S5696699 A JPS5696699 A JP S5696699A
Authority
JP
Japan
Prior art keywords
enzyme
standard
solution containing
solution
measured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP17238179A
Other languages
Japanese (ja)
Other versions
JPS5729997B2 (en
Inventor
Kiyokazu Nakano
Taiji Hashimoto
Jugoro Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP17238179A priority Critical patent/JPS5696699A/en
Publication of JPS5696699A publication Critical patent/JPS5696699A/en
Publication of JPS5729997B2 publication Critical patent/JPS5729997B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: Absorptivities of the first standard solution containing a standard substance and the second standard solution containing the same amount of water instead are measured to determine an enzyme simply and accurately.
CONSTITUTION: The first standard solution is prepared by adding a substance that is the reaction product derived from the substrate and causes absorption to the substrate in which an enzymatic reaction occurs in the presence of an enzyme to be determined. In the meantime, the second standard solution is prepared by adding the same amount of water instead of the standard substance in the first solution. The asorptivities of the first and second standard solutions are measured and the device constant is calculated from the difference between them. Then, the absorptivities of the second solution and another solution containing the same amount of a solution containing the enzyme to be determined instead of water are measured and the enzyme is determined from the difference between them and the device constant.
COPYRIGHT: (C)1981,JPO&Japio
JP17238179A 1979-12-27 1979-12-27 Determination of enzyme Granted JPS5696699A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17238179A JPS5696699A (en) 1979-12-27 1979-12-27 Determination of enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17238179A JPS5696699A (en) 1979-12-27 1979-12-27 Determination of enzyme

Publications (2)

Publication Number Publication Date
JPS5696699A true JPS5696699A (en) 1981-08-04
JPS5729997B2 JPS5729997B2 (en) 1982-06-25

Family

ID=15940850

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17238179A Granted JPS5696699A (en) 1979-12-27 1979-12-27 Determination of enzyme

Country Status (1)

Country Link
JP (1) JPS5696699A (en)

Also Published As

Publication number Publication date
JPS5729997B2 (en) 1982-06-25

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