JPS5747495A - Determination of alpha-amylase activity in serum in the coexistence of glucose - Google Patents

Determination of alpha-amylase activity in serum in the coexistence of glucose

Info

Publication number
JPS5747495A
JPS5747495A JP12243580A JP12243580A JPS5747495A JP S5747495 A JPS5747495 A JP S5747495A JP 12243580 A JP12243580 A JP 12243580A JP 12243580 A JP12243580 A JP 12243580A JP S5747495 A JPS5747495 A JP S5747495A
Authority
JP
Japan
Prior art keywords
glucose
serum
reaction system
coexistence
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12243580A
Other languages
Japanese (ja)
Other versions
JPS5733958B2 (en
Inventor
Shigeru Miwa
Norimasa Takizawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YATORON KK
Mitsubishi Kagaku Iatron Inc
Original Assignee
YATORON KK
Mitsubishi Kagaku Iatron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YATORON KK, Mitsubishi Kagaku Iatron Inc filed Critical YATORON KK
Priority to JP12243580A priority Critical patent/JPS5747495A/en
Publication of JPS5747495A publication Critical patent/JPS5747495A/en
Publication of JPS5733958B2 publication Critical patent/JPS5733958B2/ja
Granted legal-status Critical Current

Links

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: The glucose in serum is previously removed by the hexokinase reaction system, then starch is added as a substrate to measure free glucose, thus determining the activity of the titled substance rapidly and accurately.
CONSTITUTION: The glucose in serum is previously removed by the hexokinase reaction system and the hexokinase is deactivated with an anionic surfactant. A modified starch is added as a substrate and the glucose liberated by the action of α-amylase is measured colorimetrically using an enzymatic reaction system consisting of glucose oxidase, peroxidase, 4-aminoantipyrin and a coupler to determine the activity of the α-amylase in serum.
COPYRIGHT: (C)1982,JPO&Japio
JP12243580A 1980-09-05 1980-09-05 Determination of alpha-amylase activity in serum in the coexistence of glucose Granted JPS5747495A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12243580A JPS5747495A (en) 1980-09-05 1980-09-05 Determination of alpha-amylase activity in serum in the coexistence of glucose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12243580A JPS5747495A (en) 1980-09-05 1980-09-05 Determination of alpha-amylase activity in serum in the coexistence of glucose

Publications (2)

Publication Number Publication Date
JPS5747495A true JPS5747495A (en) 1982-03-18
JPS5733958B2 JPS5733958B2 (en) 1982-07-20

Family

ID=14835769

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12243580A Granted JPS5747495A (en) 1980-09-05 1980-09-05 Determination of alpha-amylase activity in serum in the coexistence of glucose

Country Status (1)

Country Link
JP (1) JPS5747495A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60106070U (en) * 1983-12-26 1985-07-19 シャープ株式会社 Solar thermal collector heat collection unit
JPS6166741U (en) * 1984-10-02 1986-05-08

Also Published As

Publication number Publication date
JPS5733958B2 (en) 1982-07-20

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