JPS5747495A - Determination of alpha-amylase activity in serum in the coexistence of glucose - Google Patents
Determination of alpha-amylase activity in serum in the coexistence of glucoseInfo
- Publication number
- JPS5747495A JPS5747495A JP12243580A JP12243580A JPS5747495A JP S5747495 A JPS5747495 A JP S5747495A JP 12243580 A JP12243580 A JP 12243580A JP 12243580 A JP12243580 A JP 12243580A JP S5747495 A JPS5747495 A JP S5747495A
- Authority
- JP
- Japan
- Prior art keywords
- glucose
- serum
- reaction system
- coexistence
- alpha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: The glucose in serum is previously removed by the hexokinase reaction system, then starch is added as a substrate to measure free glucose, thus determining the activity of the titled substance rapidly and accurately.
CONSTITUTION: The glucose in serum is previously removed by the hexokinase reaction system and the hexokinase is deactivated with an anionic surfactant. A modified starch is added as a substrate and the glucose liberated by the action of α-amylase is measured colorimetrically using an enzymatic reaction system consisting of glucose oxidase, peroxidase, 4-aminoantipyrin and a coupler to determine the activity of the α-amylase in serum.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12243580A JPS5747495A (en) | 1980-09-05 | 1980-09-05 | Determination of alpha-amylase activity in serum in the coexistence of glucose |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12243580A JPS5747495A (en) | 1980-09-05 | 1980-09-05 | Determination of alpha-amylase activity in serum in the coexistence of glucose |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5747495A true JPS5747495A (en) | 1982-03-18 |
JPS5733958B2 JPS5733958B2 (en) | 1982-07-20 |
Family
ID=14835769
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12243580A Granted JPS5747495A (en) | 1980-09-05 | 1980-09-05 | Determination of alpha-amylase activity in serum in the coexistence of glucose |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5747495A (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60106070U (en) * | 1983-12-26 | 1985-07-19 | シャープ株式会社 | Solar thermal collector heat collection unit |
JPS6166741U (en) * | 1984-10-02 | 1986-05-08 |
-
1980
- 1980-09-05 JP JP12243580A patent/JPS5747495A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5733958B2 (en) | 1982-07-20 |
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