JPS5696698A - Determination of enzyme - Google Patents
Determination of enzymeInfo
- Publication number
- JPS5696698A JPS5696698A JP17238079A JP17238079A JPS5696698A JP S5696698 A JPS5696698 A JP S5696698A JP 17238079 A JP17238079 A JP 17238079A JP 17238079 A JP17238079 A JP 17238079A JP S5696698 A JPS5696698 A JP S5696698A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- standard
- absorptivities
- standard solution
- measured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: Absorptivities of the first solution containing a standard substance and of the second standard solution containing the same amount of water instead of the standard substance are measured to determine enzymes simply and accurately.
CONSTITUTION: A mixture containing (A) a substrate that supports an enzymatic reaction in the presence of an enzyme to be determined, (B) a reagent that reacts with the reaction product to show absorptivity and (C) an enzyme catalyzing the reaction is combined with (D) the enzymatic reaction product from the substrate A as a standard substance to prepare the first standard solution. In the meantime, the same amount of water is added instead of the standard substance in the first standard solution to prepare the second standard solution. The absorptivities of the first and second solutions are measured and the device constant is calculated from the difference. Further, the absorptivities of the second standard solution and a sample containing the same amount of a solution containing the enzyme to be determined instead of water are measured and the enzyme is determined from the difference between them and the above device constant.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17238079A JPS5696698A (en) | 1979-12-27 | 1979-12-27 | Determination of enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17238079A JPS5696698A (en) | 1979-12-27 | 1979-12-27 | Determination of enzyme |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5696698A true JPS5696698A (en) | 1981-08-04 |
JPS5729996B2 JPS5729996B2 (en) | 1982-06-25 |
Family
ID=15940829
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17238079A Granted JPS5696698A (en) | 1979-12-27 | 1979-12-27 | Determination of enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5696698A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0471062A1 (en) * | 1990-03-01 | 1992-02-19 | Baxter Diagnostics Inc. | Method to use a reaction by-product as a calibrator for enzymatic assays |
-
1979
- 1979-12-27 JP JP17238079A patent/JPS5696698A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0471062A1 (en) * | 1990-03-01 | 1992-02-19 | Baxter Diagnostics Inc. | Method to use a reaction by-product as a calibrator for enzymatic assays |
EP0471062A4 (en) * | 1990-03-01 | 1992-09-09 | Baxter Diagnostics Inc. | Method to use a reaction by-product as a calibrator for enzymatic assays |
Also Published As
Publication number | Publication date |
---|---|
JPS5729996B2 (en) | 1982-06-25 |
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