JPS5669556A - Protein analyzing chemical and protein quantitative method - Google Patents

Protein analyzing chemical and protein quantitative method

Info

Publication number
JPS5669556A
JPS5669556A JP14576879A JP14576879A JPS5669556A JP S5669556 A JPS5669556 A JP S5669556A JP 14576879 A JP14576879 A JP 14576879A JP 14576879 A JP14576879 A JP 14576879A JP S5669556 A JPS5669556 A JP S5669556A
Authority
JP
Japan
Prior art keywords
protein
reagent
sample
dye
quantitative method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14576879A
Other languages
Japanese (ja)
Other versions
JPS6049256B2 (en
Inventor
Katsuyuki Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Otsuka Pharmaceutical Co Ltd
Original Assignee
Otsuka Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Otsuka Pharmaceutical Co Ltd filed Critical Otsuka Pharmaceutical Co Ltd
Priority to JP14576879A priority Critical patent/JPS6049256B2/en
Publication of JPS5669556A publication Critical patent/JPS5669556A/en
Publication of JPS6049256B2 publication Critical patent/JPS6049256B2/en
Expired legal-status Critical Current

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

PURPOSE: To provide a protein analyzing reagent and protein quantitative method including dye coomassie briliant blue. G-250, methyl cellurose and acid.
CONSTITUTION: A reagent comprises 2,500.008W0.014% kown dye reagent. briliant blue. G of formula (I), 0.01W0.03% methyl cellurose, and aicd (phosphoric acid, oxalic acid). pH is kept at 0.7W0.9 and if required, a buffering agent for stabilizing pH and a suitable solvent are added in a suitable amount. The colouring degree of the mixing liquid of the chemicals and a sample is measured by a spectrophotometer or a colorimeter to make the protein estimation. In this way, this reagent is not likely to be influenced by a substance coexisiting in the sample and the various type of protein in the sample is estimated with good regeneration and high detecting sensitivety and the mixture solution including the produced protein-dye complex is good in stability, and said reagent is suitable for detecting the protein in such as urine, blood serum, pith liquid, food.
COPYRIGHT: (C)1981,JPO&Japio
JP14576879A 1979-11-09 1979-11-09 Shiyaku for protein analysis Expired JPS6049256B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14576879A JPS6049256B2 (en) 1979-11-09 1979-11-09 Shiyaku for protein analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14576879A JPS6049256B2 (en) 1979-11-09 1979-11-09 Shiyaku for protein analysis

Publications (2)

Publication Number Publication Date
JPS5669556A true JPS5669556A (en) 1981-06-10
JPS6049256B2 JPS6049256B2 (en) 1985-10-31

Family

ID=15392705

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14576879A Expired JPS6049256B2 (en) 1979-11-09 1979-11-09 Shiyaku for protein analysis

Country Status (1)

Country Link
JP (1) JPS6049256B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5284560A (en) * 1991-06-24 1994-02-08 Wako Pure Chemical Industries, Ltd. Acid dye staining method
US9091694B2 (en) 2011-08-19 2015-07-28 Acquascience Limited Protein detection

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5284560A (en) * 1991-06-24 1994-02-08 Wako Pure Chemical Industries, Ltd. Acid dye staining method
US9091694B2 (en) 2011-08-19 2015-07-28 Acquascience Limited Protein detection
US9377467B2 (en) 2011-08-19 2016-06-28 Acquascience Limited Protein detection

Also Published As

Publication number Publication date
JPS6049256B2 (en) 1985-10-31

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