JPS5639800A - Method for specific quantitative analysis of pyruvic acid - Google Patents
Method for specific quantitative analysis of pyruvic acidInfo
- Publication number
- JPS5639800A JPS5639800A JP11490379A JP11490379A JPS5639800A JP S5639800 A JPS5639800 A JP S5639800A JP 11490379 A JP11490379 A JP 11490379A JP 11490379 A JP11490379 A JP 11490379A JP S5639800 A JPS5639800 A JP S5639800A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- pyruvic acid
- pyruvic
- amino acid
- hypotaurine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine pyruvic acid accurately even in the presence of other salts, by subjecting a pyruvic acid solutio containing a hypotaurine to the action of an ω-amino acid-pyruvic acid transaminase to generate SO2 gas.
CONSTITUTION: A pyruvic acid solution containing a hypotaurine is subjected to the action of an ω-amino acid -pyruvic acid tansaminase which is an enzyme having very high specificity for the pruvic acid. The amount of SO2 gas in th resultant substances is measured to determine the pyruvic acid specifically. The purified and crystallized ω- amino acid -pyruvic acid transaminase has a molecular weight of 167,000±3,000, shows the maximum activity at pH8.5W10.5, and is specifically active only for the pyruvic acid. Thus, the determined value is not affected even by the presence of an amino acid, e.g. glutamic acid, methionine or alanine, α-ketoglutaric acid, α-ketobutyric acid, etc.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11490379A JPS5639800A (en) | 1979-09-07 | 1979-09-07 | Method for specific quantitative analysis of pyruvic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11490379A JPS5639800A (en) | 1979-09-07 | 1979-09-07 | Method for specific quantitative analysis of pyruvic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5639800A true JPS5639800A (en) | 1981-04-15 |
JPS6319160B2 JPS6319160B2 (en) | 1988-04-21 |
Family
ID=14649507
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11490379A Granted JPS5639800A (en) | 1979-09-07 | 1979-09-07 | Method for specific quantitative analysis of pyruvic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5639800A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01139893A (en) * | 1987-11-26 | 1989-06-01 | Hakuto Kagaku Kk | Clogging preventing agent and method of suction roll for papermaking machine |
-
1979
- 1979-09-07 JP JP11490379A patent/JPS5639800A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01139893A (en) * | 1987-11-26 | 1989-06-01 | Hakuto Kagaku Kk | Clogging preventing agent and method of suction roll for papermaking machine |
Also Published As
Publication number | Publication date |
---|---|
JPS6319160B2 (en) | 1988-04-21 |
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