JPS56164786A - Production of putrescine oxidase - Google Patents

Production of putrescine oxidase

Info

Publication number
JPS56164786A
JPS56164786A JP6784680A JP6784680A JPS56164786A JP S56164786 A JPS56164786 A JP S56164786A JP 6784680 A JP6784680 A JP 6784680A JP 6784680 A JP6784680 A JP 6784680A JP S56164786 A JPS56164786 A JP S56164786A
Authority
JP
Japan
Prior art keywords
putrescine
oxidase
putrescine oxidase
culture
cultured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6784680A
Other languages
Japanese (ja)
Other versions
JPS6126356B2 (en
Inventor
Masato Okada
Hiroko Tokunaga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokuyama Corp
Original Assignee
Tokuyama Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tokuyama Corp filed Critical Tokuyama Corp
Priority to JP6784680A priority Critical patent/JPS56164786A/en
Publication of JPS56164786A publication Critical patent/JPS56164786A/en
Publication of JPS6126356B2 publication Critical patent/JPS6126356B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: A microorganism capable of producing putrescine oxidase is cultured and the putrescine oxidase is collected from the culture mixture with improvement in complication for the enzyme preparation and high Km value.
CONSTITUTION: A microorganism in Nocardia capable of producing putrescine oxidase such as Nocardia erythropolis, is cultured in a medium containing putrescine, preferably in a liquid medium aerobically. As the nutrients for the culture medium, are used common ones which are usually used to culture microorganisms. As a carbon source is used an assimilable carbohydrate such as glucose, molasses, or glycerol. The nitrogen source is, e.g., pepton, yeast extract, meat essence or ammonium sulfate. Preferably, putrescine or spermidine is added as the nitrogen source to increase the capability of putrescine oxidase production on culturing.
COPYRIGHT: (C)1981,JPO&Japio
JP6784680A 1980-05-23 1980-05-23 Production of putrescine oxidase Granted JPS56164786A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6784680A JPS56164786A (en) 1980-05-23 1980-05-23 Production of putrescine oxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6784680A JPS56164786A (en) 1980-05-23 1980-05-23 Production of putrescine oxidase

Publications (2)

Publication Number Publication Date
JPS56164786A true JPS56164786A (en) 1981-12-17
JPS6126356B2 JPS6126356B2 (en) 1986-06-20

Family

ID=13356719

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6784680A Granted JPS56164786A (en) 1980-05-23 1980-05-23 Production of putrescine oxidase

Country Status (1)

Country Link
JP (1) JPS56164786A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5948097A (en) * 1982-09-14 1984-03-19 Amano Pharmaceut Co Ltd Fractionation and determination of polyamide and acetyl-polyamine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5948097A (en) * 1982-09-14 1984-03-19 Amano Pharmaceut Co Ltd Fractionation and determination of polyamide and acetyl-polyamine
JPS6337639B2 (en) * 1982-09-14 1988-07-26 Amano Pharma Co Ltd

Also Published As

Publication number Publication date
JPS6126356B2 (en) 1986-06-20

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