JPS56158946A - Amynological measurement method of free urokinase blocking factor in specimen liquid - Google Patents

Amynological measurement method of free urokinase blocking factor in specimen liquid

Info

Publication number
JPS56158946A
JPS56158946A JP6316880A JP6316880A JPS56158946A JP S56158946 A JPS56158946 A JP S56158946A JP 6316880 A JP6316880 A JP 6316880A JP 6316880 A JP6316880 A JP 6316880A JP S56158946 A JPS56158946 A JP S56158946A
Authority
JP
Japan
Prior art keywords
urokinase
blocking
agglutination
carrier
concn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6316880A
Other languages
Japanese (ja)
Inventor
Terumi Ninagawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP6316880A priority Critical patent/JPS56158946A/en
Publication of JPS56158946A publication Critical patent/JPS56158946A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To accurately determine free urokinase blocking factors by combining carrier agglutination blocking reaction using an urokinase sensitizing carrier and an antiurokinase antibody, and a carrier agglutination reaction method using an antiurokinase antibody sensitizing carrier. CONSTITUTION:Urokinase and urokinase blocking factors exist in urine, blood, etc., in the state that they are bound to each other or the excess portion of either of these is free. An urokinase sensitizing carrier (the carrier is a red blood cell or the like) has the minimum agglutination blocking concn. of respective corpus libera of urokinase or urokinase blocking factors in the presence of an antiurokinase antibody. Hence, the minimum agglutination blocking concn. of urokinase and its blocking factors is known beforehand with a sample of a known concn., thence the agglutination reaction of the specimen liquid and an urokinase sensitizing body is attempted, whereby a minimum agglutination blocking concn. is determined. Next, the specimen liquid and the antiurokinase antibody sensitizing carrier are caused to react, and if agglutination occurs, it indicates that the above-described blocking factors exist. The concn. of the blocking factors is calculated from the dilution of the specimen liquid and the minimum agglutination blocking concn. having determined beforehand.
JP6316880A 1980-05-12 1980-05-12 Amynological measurement method of free urokinase blocking factor in specimen liquid Pending JPS56158946A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6316880A JPS56158946A (en) 1980-05-12 1980-05-12 Amynological measurement method of free urokinase blocking factor in specimen liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6316880A JPS56158946A (en) 1980-05-12 1980-05-12 Amynological measurement method of free urokinase blocking factor in specimen liquid

Publications (1)

Publication Number Publication Date
JPS56158946A true JPS56158946A (en) 1981-12-08

Family

ID=13221444

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6316880A Pending JPS56158946A (en) 1980-05-12 1980-05-12 Amynological measurement method of free urokinase blocking factor in specimen liquid

Country Status (1)

Country Link
JP (1) JPS56158946A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003973A1 (en) * 1985-01-14 1986-07-17 Terumo Kabushiki Kaisha Fibrinophilic urokinase complex and process for its preparation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003973A1 (en) * 1985-01-14 1986-07-17 Terumo Kabushiki Kaisha Fibrinophilic urokinase complex and process for its preparation

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