JPS5615685A - Induction of l-form bacterium of hemolytic streptococcus - Google Patents
Induction of l-form bacterium of hemolytic streptococcusInfo
- Publication number
- JPS5615685A JPS5615685A JP8931379A JP8931379A JPS5615685A JP S5615685 A JPS5615685 A JP S5615685A JP 8931379 A JP8931379 A JP 8931379A JP 8931379 A JP8931379 A JP 8931379A JP S5615685 A JPS5615685 A JP S5615685A
- Authority
- JP
- Japan
- Prior art keywords
- bacterium
- hemolytic
- culture medium
- hemolytic streptococcus
- cell wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain an L-form bacterium having a good antitumor activity and no side effects, e.g. phlogogenic and pyrogenic properties, by subjecting a bacterium body of a hemolytic streptococcus to the action of a hemolytic enzyme, and incubating the resulting protoplast in a culture medium containing a cell wall synthesis inhibitor.
CONSTITUTION: The bacterium body of a hemolytic streptococcus is subjected to the action of a hemolytic enzyme to form a plastoplast, which is then incubated in a culture medium containing a cell wall synthesis inhibitor to give an L-form bacterium of the hemolytic streptococcus. For example, a bacterium body of a hemolytic streptococcus is suspended in a hypertonic solution, and a hemolytic enzyme is added to the solution and treated at 10W40°C for 10W120min. The resulting protoplast is then incubated in a solid culture medium for the L-form bacterium containing an inhibitor for the cell wall synthesis, e.g. penicillin, at 25W40°C. In the incubation, the hemolytic streptococcus is in oculated in the solid culture medium for the L-form bacterium, and a paper disk impregnated with the inhibitor tfor the cell wall synthesis is placed on the culture medium to incubate the protoplast effectively.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8931379A JPS5615685A (en) | 1979-07-16 | 1979-07-16 | Induction of l-form bacterium of hemolytic streptococcus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8931379A JPS5615685A (en) | 1979-07-16 | 1979-07-16 | Induction of l-form bacterium of hemolytic streptococcus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5615685A true JPS5615685A (en) | 1981-02-14 |
| JPS6359676B2 JPS6359676B2 (en) | 1988-11-21 |
Family
ID=13967170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8931379A Granted JPS5615685A (en) | 1979-07-16 | 1979-07-16 | Induction of l-form bacterium of hemolytic streptococcus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5615685A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6030677A (en) * | 1983-08-01 | 1985-02-16 | Juzo Udaka | Cultivation of bacteria belonging to streptococcus genus |
-
1979
- 1979-07-16 JP JP8931379A patent/JPS5615685A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6030677A (en) * | 1983-08-01 | 1985-02-16 | Juzo Udaka | Cultivation of bacteria belonging to streptococcus genus |
| JPH026511B2 (en) * | 1983-08-01 | 1990-02-09 | Juzo Udaka |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6359676B2 (en) | 1988-11-21 |
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