JPS56154992A - Heat-resistant immobilized acetate kinase - Google Patents

Heat-resistant immobilized acetate kinase

Info

Publication number
JPS56154992A
JPS56154992A JP5768280A JP5768280A JPS56154992A JP S56154992 A JPS56154992 A JP S56154992A JP 5768280 A JP5768280 A JP 5768280A JP 5768280 A JP5768280 A JP 5768280A JP S56154992 A JPS56154992 A JP S56154992A
Authority
JP
Japan
Prior art keywords
acetate kinase
heat
resistant
immobilization
collected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5768280A
Other languages
Japanese (ja)
Other versions
JPS5933355B2 (en
Inventor
Kazutomo Imahori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN Institute of Physical and Chemical Research
Original Assignee
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN Institute of Physical and Chemical Research filed Critical RIKEN Institute of Physical and Chemical Research
Priority to JP5768280A priority Critical patent/JPS5933355B2/en
Publication of JPS56154992A publication Critical patent/JPS56154992A/en
Publication of JPS5933355B2 publication Critical patent/JPS5933355B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: Heat-resistant acetate kinase is collected from a thermophilus Bacillus bacterium and made to react with a carrier of porous glass for immobilization, thus producing the titled substance showing high enzymatic activity after immobilization.
CONSTITUTION: Bacillus stearothermophilus or the like is cultured and acetate kinase is collected at any stage, e.g., from the culture mixture, culture filtrate or separated cells or obtained as the purified product. A previously activated carrier for immobilization such as porous glass, polysaccharide derivative or polyacrylic acid amide and the above heat-resistant acetate kinase are dispersed in a buffer solution of 6W9.5 pH and they are stirred at 0W70°C, when necessary irradiated with UV or visible rays to give the objective heat-resistant immobilized acetate kinase.
COPYRIGHT: (C)1981,JPO&Japio
JP5768280A 1980-04-30 1980-04-30 Immobilized thermostable acetate kinase Expired JPS5933355B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5768280A JPS5933355B2 (en) 1980-04-30 1980-04-30 Immobilized thermostable acetate kinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5768280A JPS5933355B2 (en) 1980-04-30 1980-04-30 Immobilized thermostable acetate kinase

Publications (2)

Publication Number Publication Date
JPS56154992A true JPS56154992A (en) 1981-11-30
JPS5933355B2 JPS5933355B2 (en) 1984-08-15

Family

ID=13062694

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5768280A Expired JPS5933355B2 (en) 1980-04-30 1980-04-30 Immobilized thermostable acetate kinase

Country Status (1)

Country Link
JP (1) JPS5933355B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4886749A (en) * 1986-05-28 1989-12-12 Unitika Ltd. Process for producing diadenosine tetraphosphate and derivatives thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0418266Y2 (en) * 1986-09-30 1992-04-23

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4886749A (en) * 1986-05-28 1989-12-12 Unitika Ltd. Process for producing diadenosine tetraphosphate and derivatives thereof

Also Published As

Publication number Publication date
JPS5933355B2 (en) 1984-08-15

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