JPS5523401A - Measuring method for lipid peroxide - Google Patents

Measuring method for lipid peroxide

Info

Publication number
JPS5523401A
JPS5523401A JP2231378A JP2231378A JPS5523401A JP S5523401 A JPS5523401 A JP S5523401A JP 2231378 A JP2231378 A JP 2231378A JP 2231378 A JP2231378 A JP 2231378A JP S5523401 A JPS5523401 A JP S5523401A
Authority
JP
Japan
Prior art keywords
valences
coloration
reagents
approximate
kinds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2231378A
Other languages
Japanese (ja)
Inventor
Kan Kasahara
Kazuhiko Yamanishi
Toshiro Hanada
Kuniaki Tokuda
Shinzo Obata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP2231378A priority Critical patent/JPS5523401A/en
Priority to DE19782856487 priority patent/DE2856487A1/en
Priority to FR7836821A priority patent/FR2413660A1/en
Priority to US05/974,148 priority patent/US4295853A/en
Publication of JPS5523401A publication Critical patent/JPS5523401A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: To make it possible to measure accurately and rapidly lipid peroxide in an organism sample through simple opration by making use of oxidization which increases remarkably fat peroxid extracted by a solvent while a metal compound of not less than two kinds of valences resides.
CONSTITUTION: An organism sample such as blood sedimentation of approximate 20W200μλ is mixed with an organic solvent such as isopropyl alcohol by stirring and after centrifugal precipitation, the supernatant fluid is separated. To the supernatant fluid, a solution of oxidizable colration reagents, e.g. 4-aminoantipyrin and 3-methyl-N-ethyl-N(β-hydroxyethyl)-aniline and a solution of a metal compound such as FeCl3 with not less than two kinds of valences are added and held at a temperature of approximate 37°C for the measurement of coloration. Consequently, the need of protein removal is eliminated and the measurement is fulfilled by using conventional oxidizable coloration reagents.
COPYRIGHT: (C)1980,JPO&Japio
JP2231378A 1977-12-29 1978-02-28 Measuring method for lipid peroxide Pending JPS5523401A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2231378A JPS5523401A (en) 1978-02-28 1978-02-28 Measuring method for lipid peroxide
DE19782856487 DE2856487A1 (en) 1977-12-29 1978-12-28 PROCEDURE FOR DETERMINING PEROXID SUBSTANCES AND COMPOUNDS THAT CAN BE USED THEREFORE
FR7836821A FR2413660A1 (en) 1977-12-29 1978-12-29 METHOD FOR DETERMINING ONE OR MORE PEROXIDIC SUBSTANCES
US05/974,148 US4295853A (en) 1977-12-29 1978-12-29 Method for measuring peroxidic substances

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2231378A JPS5523401A (en) 1978-02-28 1978-02-28 Measuring method for lipid peroxide

Publications (1)

Publication Number Publication Date
JPS5523401A true JPS5523401A (en) 1980-02-19

Family

ID=12079237

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2231378A Pending JPS5523401A (en) 1977-12-29 1978-02-28 Measuring method for lipid peroxide

Country Status (1)

Country Link
JP (1) JPS5523401A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851353A (en) * 1980-04-14 1989-07-25 Kyowa Hakko Kogyo Co., Ltd. Method and test composition for determination of lipid peroxide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851353A (en) * 1980-04-14 1989-07-25 Kyowa Hakko Kogyo Co., Ltd. Method and test composition for determination of lipid peroxide

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