JPS5523401A - Measuring method for lipid peroxide - Google Patents
Measuring method for lipid peroxideInfo
- Publication number
- JPS5523401A JPS5523401A JP2231378A JP2231378A JPS5523401A JP S5523401 A JPS5523401 A JP S5523401A JP 2231378 A JP2231378 A JP 2231378A JP 2231378 A JP2231378 A JP 2231378A JP S5523401 A JPS5523401 A JP S5523401A
- Authority
- JP
- Japan
- Prior art keywords
- valences
- coloration
- reagents
- approximate
- kinds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To make it possible to measure accurately and rapidly lipid peroxide in an organism sample through simple opration by making use of oxidization which increases remarkably fat peroxid extracted by a solvent while a metal compound of not less than two kinds of valences resides.
CONSTITUTION: An organism sample such as blood sedimentation of approximate 20W200μλ is mixed with an organic solvent such as isopropyl alcohol by stirring and after centrifugal precipitation, the supernatant fluid is separated. To the supernatant fluid, a solution of oxidizable colration reagents, e.g. 4-aminoantipyrin and 3-methyl-N-ethyl-N(β-hydroxyethyl)-aniline and a solution of a metal compound such as FeCl3 with not less than two kinds of valences are added and held at a temperature of approximate 37°C for the measurement of coloration. Consequently, the need of protein removal is eliminated and the measurement is fulfilled by using conventional oxidizable coloration reagents.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2231378A JPS5523401A (en) | 1978-02-28 | 1978-02-28 | Measuring method for lipid peroxide |
DE19782856487 DE2856487A1 (en) | 1977-12-29 | 1978-12-28 | PROCEDURE FOR DETERMINING PEROXID SUBSTANCES AND COMPOUNDS THAT CAN BE USED THEREFORE |
FR7836821A FR2413660A1 (en) | 1977-12-29 | 1978-12-29 | METHOD FOR DETERMINING ONE OR MORE PEROXIDIC SUBSTANCES |
US05/974,148 US4295853A (en) | 1977-12-29 | 1978-12-29 | Method for measuring peroxidic substances |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2231378A JPS5523401A (en) | 1978-02-28 | 1978-02-28 | Measuring method for lipid peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5523401A true JPS5523401A (en) | 1980-02-19 |
Family
ID=12079237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2231378A Pending JPS5523401A (en) | 1977-12-29 | 1978-02-28 | Measuring method for lipid peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5523401A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4851353A (en) * | 1980-04-14 | 1989-07-25 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for determination of lipid peroxide |
-
1978
- 1978-02-28 JP JP2231378A patent/JPS5523401A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4851353A (en) * | 1980-04-14 | 1989-07-25 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for determination of lipid peroxide |
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