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YATORON KK
Mitsubishi Kagaku Iatron Inc
Original Assignee
YATORON KK
Mitsubishi Kagaku Iatron Inc
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Filing date
Publication date
Application filed by YATORON KK, Mitsubishi Kagaku Iatron IncfiledCriticalYATORON KK
Priority to JP8130179ApriorityCriticalpatent/JPS568699A/en
Publication of JPS568699ApublicationCriticalpatent/JPS568699A/en
Publication of JPS5636920B2publicationCriticalpatent/JPS5636920B2/ja
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
PURPOSE: To determine uric acid in blood serum using uricase/peroxidase/hydrogen-donor system, accurately, without interference of ascorbic acid, by removing ascorbic acid in the blood serum with Cu2+ and Fe2+ before the determination.
CONSTITUTION: Ascorbic acid is removed from blood serum containing uric acid by adding 0.2W0.5mmol/l of Cu2+ and 0.02W0.06mmol/l of Fe2+to the serum at pH3W5. After removing ascorbic acid, uric acid in the serum is determined by the use of a reaction system comprising uricase, peroxidase and a hydrogen donor. The method enables the accurate and reproducible determination of uric acid in a specimen containing a large amount of ascorbic acid which is a strong reducing substance, without the interference of ascorbic acid.
COPYRIGHT: (C)1981,JPO&Japio
JP8130179A1979-06-291979-06-29Novel method for determination of uric acid in blood serum
GrantedJPS568699A
(en)
Diagnostic means for the determination of ammonia or of substrates liberating ammonia in reacting, new ph-indicators used therein and method for the determination of ammonia or the above-mentioned substrates
Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances