JPS55141199A - Qualitative determination of spermidine and putrescine - Google Patents
Qualitative determination of spermidine and putrescineInfo
- Publication number
- JPS55141199A JPS55141199A JP4718379A JP4718379A JPS55141199A JP S55141199 A JPS55141199 A JP S55141199A JP 4718379 A JP4718379 A JP 4718379A JP 4718379 A JP4718379 A JP 4718379A JP S55141199 A JPS55141199 A JP S55141199A
- Authority
- JP
- Japan
- Prior art keywords
- spermidine
- solution
- putrescine
- electron acceptor
- quickly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine spermidine with good reproducibility quickly and easily, by reacting a spermidine-containing solution with spermidine dehydrogenase in the pressence of an electron acceptor.
CONSTITUTION: A spermidine-containing solution, e.g., the human urine, an extracted solution of the human organization, is reacted usually with 0.1W5.0 enzyme units of spermidine dehydrogenase in the presence of an electron acceptor, e.g., potassium ferricyamide. A SH group-containing compound (e.g., β-mercaptoethanol) is then added to the reaction solution to reduce the excess electron acceptor so that the reaction solution becomes colorless. Prepared 1-pyrroline shown by the formla 1 is submitted to colorimetry to determine spermidine. By using this determination of sperimidine, a solution containing both spermidine and putrescine can be analyzed quantitatively to determine putrescine with good reproducibility quickly and easily.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4718379A JPS55141199A (en) | 1979-04-19 | 1979-04-19 | Qualitative determination of spermidine and putrescine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4718379A JPS55141199A (en) | 1979-04-19 | 1979-04-19 | Qualitative determination of spermidine and putrescine |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55141199A true JPS55141199A (en) | 1980-11-04 |
JPS624119B2 JPS624119B2 (en) | 1987-01-28 |
Family
ID=12767965
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4718379A Granted JPS55141199A (en) | 1979-04-19 | 1979-04-19 | Qualitative determination of spermidine and putrescine |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55141199A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7319038B2 (en) * | 2002-09-19 | 2008-01-15 | The Johns Hopkins University | Sensor for monitoring an analyte |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5067193A (en) * | 1973-10-16 | 1975-06-05 |
-
1979
- 1979-04-19 JP JP4718379A patent/JPS55141199A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5067193A (en) * | 1973-10-16 | 1975-06-05 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7319038B2 (en) * | 2002-09-19 | 2008-01-15 | The Johns Hopkins University | Sensor for monitoring an analyte |
Also Published As
Publication number | Publication date |
---|---|
JPS624119B2 (en) | 1987-01-28 |
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