JPS57147058A - Measuring method for hydrogen peroxide - Google Patents

Measuring method for hydrogen peroxide

Info

Publication number
JPS57147058A
JPS57147058A JP3226681A JP3226681A JPS57147058A JP S57147058 A JPS57147058 A JP S57147058A JP 3226681 A JP3226681 A JP 3226681A JP 3226681 A JP3226681 A JP 3226681A JP S57147058 A JPS57147058 A JP S57147058A
Authority
JP
Japan
Prior art keywords
solution
uricase
peroxidase
hydrogen peroxide
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3226681A
Other languages
Japanese (ja)
Inventor
Kazuhiko Yamanishi
Toshiro Hanada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP3226681A priority Critical patent/JPS57147058A/en
Publication of JPS57147058A publication Critical patent/JPS57147058A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To carry out the accurate calorimetry without being affected by bilirubin, etc. by measuring hydrogen peroxide produced by uric acid.uricase reaction, etc. under the existence of metallic azide compound. CONSTITUTION:An uricase solution containing uricase, 4-amino antipyrin in 0.05M phosphoric acid buffer solution (8.0pH) is used for the uricase solution and a peroxidase solution containing peroxidase, 3-methyl-N-ethyl-N-hydroxyethyl aniline in 0.15M phosphoric acid buffer solution (5.0pH) is used for the peroxidase solution and then, 550nm wavelength is used for measuring the wavelength. 100mul blood serum or a standard solution of uric acid, is sampled and 1.0ml uricase solution is added and then, is mixed. Further, reaction is carried out for a constant time in a 27 deg.C thermostat and next, 20ml peroxidase solution is added and is mixed. Absorbance is measured after reacting two minutes in said thermostat and it is shown by the figure. The accurate calorimetry is carried out from this result because the uricase solution added sodium azide does not decompose hydrogen peroxide.
JP3226681A 1981-03-06 1981-03-06 Measuring method for hydrogen peroxide Pending JPS57147058A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3226681A JPS57147058A (en) 1981-03-06 1981-03-06 Measuring method for hydrogen peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3226681A JPS57147058A (en) 1981-03-06 1981-03-06 Measuring method for hydrogen peroxide

Publications (1)

Publication Number Publication Date
JPS57147058A true JPS57147058A (en) 1982-09-10

Family

ID=12354197

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3226681A Pending JPS57147058A (en) 1981-03-06 1981-03-06 Measuring method for hydrogen peroxide

Country Status (1)

Country Link
JP (1) JPS57147058A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60262598A (en) * 1984-06-04 1985-12-25 マイルス・インコーポレーテッド Test kit for measuring presence of ethanol in aqueous specimen, its production and measuring method
JPS63163164A (en) * 1986-12-25 1988-07-06 Konica Corp Multi-layer analysis element
WO2012081539A1 (en) * 2010-12-13 2012-06-21 協和メデックス株式会社 Method for analysis for component to be assayed

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60262598A (en) * 1984-06-04 1985-12-25 マイルス・インコーポレーテッド Test kit for measuring presence of ethanol in aqueous specimen, its production and measuring method
JPH0586197B2 (en) * 1984-06-04 1993-12-10 Miles Inc
JPS63163164A (en) * 1986-12-25 1988-07-06 Konica Corp Multi-layer analysis element
WO2012081539A1 (en) * 2010-12-13 2012-06-21 協和メデックス株式会社 Method for analysis for component to be assayed
US9671348B2 (en) 2010-12-13 2017-06-06 Kyowa Medex Co., Ltd. Method for measuring component by treating aqueous sample with alpha-keto acid and then converting component to hydrogen peroxide

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