JPS55127988A - Preparation of peptidase - Google Patents
Preparation of peptidaseInfo
- Publication number
- JPS55127988A JPS55127988A JP3319179A JP3319179A JPS55127988A JP S55127988 A JPS55127988 A JP S55127988A JP 3319179 A JP3319179 A JP 3319179A JP 3319179 A JP3319179 A JP 3319179A JP S55127988 A JPS55127988 A JP S55127988A
- Authority
- JP
- Japan
- Prior art keywords
- peptidase
- precipitate
- bodies
- flavobacterium
- genus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To prepare peptidase, which is an enzyme of the endo type hydrolyzing the peptide bonds between proline and other amino acids specifically, from the bodies of peptidase-producing bacteria belonging to the genus Flavo bacterium.
CONSTITUTION: Peptidase-producing bacteria, e.g. Flavobacterium TY-78-74 (FERM-P No.4849), which belong to the genus Flavobacterium, are incubated, and the bacterium bodies are destroyed to obtain the cell fluid, which is salted out with ammonium sulfate to form a precipitate. The precipitate is subjected to chromatography with a cation exchanger to separate a peptidase-containing fraction, which is passed through a hydroxy apatite column, and purified by gel filtration.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3319179A JPS55127988A (en) | 1979-03-23 | 1979-03-23 | Preparation of peptidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3319179A JPS55127988A (en) | 1979-03-23 | 1979-03-23 | Preparation of peptidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55127988A true JPS55127988A (en) | 1980-10-03 |
JPS6156997B2 JPS6156997B2 (en) | 1986-12-04 |
Family
ID=12379584
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3319179A Granted JPS55127988A (en) | 1979-03-23 | 1979-03-23 | Preparation of peptidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55127988A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0104554A2 (en) * | 1982-09-28 | 1984-04-04 | Miles Laboratories, Inc. | Preparation of an alkaline protease from flavobacterium arborescens |
WO1996001894A1 (en) * | 1994-07-08 | 1996-01-25 | Ibex Technologies R And D, Inc. | Chondroitin lyase enzymes |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03295595A (en) * | 1990-04-12 | 1991-12-26 | Matsushita Electric Ind Co Ltd | Steam iron |
-
1979
- 1979-03-23 JP JP3319179A patent/JPS55127988A/en active Granted
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0104554A2 (en) * | 1982-09-28 | 1984-04-04 | Miles Laboratories, Inc. | Preparation of an alkaline protease from flavobacterium arborescens |
WO1996001894A1 (en) * | 1994-07-08 | 1996-01-25 | Ibex Technologies R And D, Inc. | Chondroitin lyase enzymes |
US6054569A (en) * | 1994-07-08 | 2000-04-25 | Ibex Technologies R And D, Inc. | Chondroitin lyase enzymes |
US6093563A (en) * | 1994-07-08 | 2000-07-25 | Ibex Technologies R And D, Inc. | Chondroitin lyase enzymes |
EP1607478A3 (en) * | 1994-07-08 | 2006-02-22 | BioMarin Pharmaceutical Inc. | Chondroitin lyase enzymes |
Also Published As
Publication number | Publication date |
---|---|
JPS6156997B2 (en) | 1986-12-04 |
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