JPS5473187A - Purification of beta-amylase produced by bacillus - Google Patents
Purification of beta-amylase produced by bacillusInfo
- Publication number
- JPS5473187A JPS5473187A JP14093177A JP14093177A JPS5473187A JP S5473187 A JPS5473187 A JP S5473187A JP 14093177 A JP14093177 A JP 14093177A JP 14093177 A JP14093177 A JP 14093177A JP S5473187 A JPS5473187 A JP S5473187A
- Authority
- JP
- Japan
- Prior art keywords
- amylase
- bacillus
- amylase produced
- beta
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To purify β-amylase produced by bacteria Belonging to bacillus genus, economically, in high efficiency, by using aluminum oxide as absorbent and solution of citric acid salt as eluant.
CONSTITUTION: β-Amylase produced by bacteria belonging to Bacillus genus, e.g. Bacillus cereus var. mycoides (FERM-P No. 2391) is adsorbed to activated alumina at a pH of 4W9, pref. 5W8, and eluted the absorbed β-amylase with an aqueous solution of sodium citrate or potassium citrate. The concentration of the salt is usually 0.01W0.05M, and the amount of adsorbed β-amylase is about 10.000 unit/g of alumina having particle size of 100W200 mesh.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14093177A JPS5473187A (en) | 1977-11-22 | 1977-11-22 | Purification of beta-amylase produced by bacillus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14093177A JPS5473187A (en) | 1977-11-22 | 1977-11-22 | Purification of beta-amylase produced by bacillus |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5473187A true JPS5473187A (en) | 1979-06-12 |
JPS563031B2 JPS563031B2 (en) | 1981-01-22 |
Family
ID=15280137
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP14093177A Granted JPS5473187A (en) | 1977-11-22 | 1977-11-22 | Purification of beta-amylase produced by bacillus |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5473187A (en) |
-
1977
- 1977-11-22 JP JP14093177A patent/JPS5473187A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS563031B2 (en) | 1981-01-22 |
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