JPS5473187A - Purification of beta-amylase produced by bacillus - Google Patents

Purification of beta-amylase produced by bacillus

Info

Publication number
JPS5473187A
JPS5473187A JP14093177A JP14093177A JPS5473187A JP S5473187 A JPS5473187 A JP S5473187A JP 14093177 A JP14093177 A JP 14093177A JP 14093177 A JP14093177 A JP 14093177A JP S5473187 A JPS5473187 A JP S5473187A
Authority
JP
Japan
Prior art keywords
amylase
bacillus
amylase produced
beta
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14093177A
Other languages
Japanese (ja)
Other versions
JPS563031B2 (en
Inventor
Yoshiyuki Takasaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP14093177A priority Critical patent/JPS5473187A/en
Publication of JPS5473187A publication Critical patent/JPS5473187A/en
Publication of JPS563031B2 publication Critical patent/JPS563031B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To purify β-amylase produced by bacteria Belonging to bacillus genus, economically, in high efficiency, by using aluminum oxide as absorbent and solution of citric acid salt as eluant.
CONSTITUTION: β-Amylase produced by bacteria belonging to Bacillus genus, e.g. Bacillus cereus var. mycoides (FERM-P No. 2391) is adsorbed to activated alumina at a pH of 4W9, pref. 5W8, and eluted the absorbed β-amylase with an aqueous solution of sodium citrate or potassium citrate. The concentration of the salt is usually 0.01W0.05M, and the amount of adsorbed β-amylase is about 10.000 unit/g of alumina having particle size of 100W200 mesh.
COPYRIGHT: (C)1979,JPO&Japio
JP14093177A 1977-11-22 1977-11-22 Purification of beta-amylase produced by bacillus Granted JPS5473187A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14093177A JPS5473187A (en) 1977-11-22 1977-11-22 Purification of beta-amylase produced by bacillus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14093177A JPS5473187A (en) 1977-11-22 1977-11-22 Purification of beta-amylase produced by bacillus

Publications (2)

Publication Number Publication Date
JPS5473187A true JPS5473187A (en) 1979-06-12
JPS563031B2 JPS563031B2 (en) 1981-01-22

Family

ID=15280137

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14093177A Granted JPS5473187A (en) 1977-11-22 1977-11-22 Purification of beta-amylase produced by bacillus

Country Status (1)

Country Link
JP (1) JPS5473187A (en)

Also Published As

Publication number Publication date
JPS563031B2 (en) 1981-01-22

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