JPS54101486A - Purification of urokinase - Google Patents
Purification of urokinaseInfo
- Publication number
- JPS54101486A JPS54101486A JP776278A JP776278A JPS54101486A JP S54101486 A JPS54101486 A JP S54101486A JP 776278 A JP776278 A JP 776278A JP 776278 A JP776278 A JP 776278A JP S54101486 A JPS54101486 A JP S54101486A
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- high purity
- substances
- crosslinked agarose
- crude
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare a high purity urokinase which can be used as a component of an injection without further purification, by selectively adsorbing and removing pyrogenic substances and other impurities from a crude urokinase with diethyllaminoethylagarose.
CONSTITUTION: A crude urokinase solution is treated with a crosslinked agarose buffered with a buffer solution having a concentration of 0.01W0.1M and pH of 7W8. Pyrogenic substances and colored substances are selectively adsorbed to the adsorbent and removed. The crosslinked agarose is, e.g. DEAE-Sepharose CL-6B. A high purity, colorless, clear urokinase can be obtained at a recovery of ≥90%.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP776278A JPS54101486A (en) | 1978-01-25 | 1978-01-25 | Purification of urokinase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP776278A JPS54101486A (en) | 1978-01-25 | 1978-01-25 | Purification of urokinase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54101486A true JPS54101486A (en) | 1979-08-10 |
Family
ID=11674691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP776278A Pending JPS54101486A (en) | 1978-01-25 | 1978-01-25 | Purification of urokinase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54101486A (en) |
-
1978
- 1978-01-25 JP JP776278A patent/JPS54101486A/en active Pending
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