JPS54101486A - Purification of urokinase - Google Patents

Purification of urokinase

Info

Publication number
JPS54101486A
JPS54101486A JP776278A JP776278A JPS54101486A JP S54101486 A JPS54101486 A JP S54101486A JP 776278 A JP776278 A JP 776278A JP 776278 A JP776278 A JP 776278A JP S54101486 A JPS54101486 A JP S54101486A
Authority
JP
Japan
Prior art keywords
urokinase
high purity
substances
crosslinked agarose
crude
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP776278A
Other languages
Japanese (ja)
Inventor
Hideki Yanagi
Yasuo Uta
Junichi Yoshikawa
Shigeo Ogino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
Original Assignee
Sumitomo Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Priority to JP776278A priority Critical patent/JPS54101486A/en
Publication of JPS54101486A publication Critical patent/JPS54101486A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare a high purity urokinase which can be used as a component of an injection without further purification, by selectively adsorbing and removing pyrogenic substances and other impurities from a crude urokinase with diethyllaminoethylagarose.
CONSTITUTION: A crude urokinase solution is treated with a crosslinked agarose buffered with a buffer solution having a concentration of 0.01W0.1M and pH of 7W8. Pyrogenic substances and colored substances are selectively adsorbed to the adsorbent and removed. The crosslinked agarose is, e.g. DEAE-Sepharose CL-6B. A high purity, colorless, clear urokinase can be obtained at a recovery of ≥90%.
COPYRIGHT: (C)1979,JPO&Japio
JP776278A 1978-01-25 1978-01-25 Purification of urokinase Pending JPS54101486A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP776278A JPS54101486A (en) 1978-01-25 1978-01-25 Purification of urokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP776278A JPS54101486A (en) 1978-01-25 1978-01-25 Purification of urokinase

Publications (1)

Publication Number Publication Date
JPS54101486A true JPS54101486A (en) 1979-08-10

Family

ID=11674691

Family Applications (1)

Application Number Title Priority Date Filing Date
JP776278A Pending JPS54101486A (en) 1978-01-25 1978-01-25 Purification of urokinase

Country Status (1)

Country Link
JP (1) JPS54101486A (en)

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