JPS5733595A - Purification of urokinase - Google Patents

Purification of urokinase

Info

Publication number
JPS5733595A
JPS5733595A JP10871480A JP10871480A JPS5733595A JP S5733595 A JPS5733595 A JP S5733595A JP 10871480 A JP10871480 A JP 10871480A JP 10871480 A JP10871480 A JP 10871480A JP S5733595 A JPS5733595 A JP S5733595A
Authority
JP
Japan
Prior art keywords
solution
adsorb
ion
urokinase
exchanger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10871480A
Other languages
Japanese (ja)
Inventor
Masaaki Kato
Hiroyasu Suzuki
Masahiro Higuchi
Yoshiaki Ishimatsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Denka Co Ltd
Original Assignee
Denki Kagaku Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Denki Kagaku Kogyo KK filed Critical Denki Kagaku Kogyo KK
Priority to JP10871480A priority Critical patent/JPS5733595A/en
Publication of JPS5733595A publication Critical patent/JPS5733595A/en
Pending legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: An ion-exchanger containing diethylaminoethyl groups is made to adsorb a quaternary ammonium salt solution and brought into contact with a crude urokinase solution to adsorb impurities, thus producing the titled substance of high purity rapidly and easily.
CONSTITUTION: An ion-exchanger containing diethylaminoethyl groups that is composed of a high polymeric polysaccharide such as diethylaminoethyl group-containing cellulose or crosslinked dextran is made to adsorb a quaternary ammonium salt solution. For example, a cationic surfactant such as stearyltrimethylammonium chloride or an amphoteric surfactant is used and the unadsorbed salt is removed with a buffer solution of pH5W8. Then, a crude urokinase solution is brought into contact with the resultant ion-exchanger to effect the adsorption of impurities and obtain the purified urokinase.
COPYRIGHT: (C)1982,JPO&Japio
JP10871480A 1980-08-07 1980-08-07 Purification of urokinase Pending JPS5733595A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10871480A JPS5733595A (en) 1980-08-07 1980-08-07 Purification of urokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10871480A JPS5733595A (en) 1980-08-07 1980-08-07 Purification of urokinase

Publications (1)

Publication Number Publication Date
JPS5733595A true JPS5733595A (en) 1982-02-23

Family

ID=14491721

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10871480A Pending JPS5733595A (en) 1980-08-07 1980-08-07 Purification of urokinase

Country Status (1)

Country Link
JP (1) JPS5733595A (en)

Similar Documents

Publication Publication Date Title
JPS52100500A (en) Purification and concentration of aqueous stevioside extract
ES525361A0 (en) PROCEDURE TO PREPARE A SAFE MEDICATION AGAINST HEPATITIS CONTAINING PROTEIN CALLED INACTIVATOR CL.
JPS5733595A (en) Purification of urokinase
GB902639A (en) Method of purification of streptokinase
JPS5517364A (en) Purification of pancreatic kallikrein
HUT35471A (en) Herbicide preparations containing antidote of n-/dichlor-acetyl/-n-substituted.glycine-n', n-disubstituted-amide-type, or containing agent and antidote, as well as process for the production of the antidote
JPS51126399A (en) Purification of sodium hydroxide
JPS54101486A (en) Purification of urokinase
JPS5599191A (en) Purification of kallikrein in human urine
JPS5212984A (en) Purification of urokinase
JPS52128294A (en) Separation and purification of cephalosporins
JPS57150382A (en) Purification of super oxide dismutase
JPS52797A (en) Purification process of solution of sodium hypochlorite
JPS5513002A (en) Preparation of urokinase
JPS57203015A (en) Purifying method of human urinary callicrein
JPS5211359A (en) Manufacturing process of spring washer and its washer
JPS55150900A (en) Production of interferon
JPS51136784A (en) Method f or preparing a polymer containing a a skelton of stabilizer in the molecule
JPS6485077A (en) Purification of arginine residue-specific endopeptidase
JPS51117995A (en) Hidrogen purifying process
JPS5581587A (en) Purification of kallikrein
JPS5420191A (en) Purification of urokinase
JPS5259081A (en) Method of purifying liquid crystal
JPS55304A (en) Purification of hbs antigen
JPS5264697A (en) Process for treating single area