JPH11512616A - 抗線虫性バチルス・チューリンギエンシス(Bacillus thuringiensis)遺伝子、毒素、および単離株の同定およびその利用 - Google Patents
抗線虫性バチルス・チューリンギエンシス(Bacillus thuringiensis)遺伝子、毒素、および単離株の同定およびその利用Info
- Publication number
- JPH11512616A JPH11512616A JP9514366A JP51436697A JPH11512616A JP H11512616 A JPH11512616 A JP H11512616A JP 9514366 A JP9514366 A JP 9514366A JP 51436697 A JP51436697 A JP 51436697A JP H11512616 A JPH11512616 A JP H11512616A
- Authority
- JP
- Japan
- Prior art keywords
- nematode
- toxin
- seq
- nrrl
- bacillus thuringiensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- C—CHEMISTRY; METALLURGY
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.PS32B(NRRL B-21531)、PS49C(NRRL B-21532)、PS52E3(NRRL B-21533)、PS 54G2(NRRL B- )、PS10ICC3(NRRL B-21534)、PS178D4(NRRL B- )、PS185L2(N RRL B-21535)、PS197P3(NRRL B-21536)、PS242B6(NRRL B-21537)、PS242G4(NRRL B-21538)、PS242H10(NRRL B-21539)、PS242K17(NRRL B-21540)、PS244A2(NRRL B-21541)、PS244D1(NRRL B-21542)、およびそれらの変異体からなる群より選択 される単離株の同定されている特徴を有する、新規のバチルス・チューリンギエ ンシス(Bacillus thuringiensis)単離株。 2.PS32B、PS49C、PS52E3、PS54G2、PS101CC3、PS178D4、PS185L2、PS197P3 、PS242B6、PS242G4、PS242H10、PS242K17、PS244A2、PS244D1およびそれらの変 異体、または該毒素の抗線虫性断片もしくは変異体からなる群より選択されるバ チルス・チューリンギエンシス(Bacillus thuringiensis)単離株に由来する抗 線虫性毒素。 3.バチルス・チューリンギエンシス単離株がPS32Bである、請求項2記載の 抗線虫性毒素。 4.バチルス・チューリンギエンシス単離株がPS49Cである、請求項2記載の 抗線虫性毒素。 5.バチルス・チューリンギエンシス単離株がPS52E3またはその変異体である 、請求項2記載の抗線虫性毒素。 6.バチルス・チューリンギエンシス単離株がPS54G2である、請求項2記載の 抗線虫性毒素。 7.バチルス・チューリンギエンシス単離株がPS101CC3である、請求項2記載 の抗線虫性毒素。 8.バチルス・チューリンギエンシス単離株がPS178D4である、請求項2記載 の抗線虫性毒素。 9.バチルス・チューリンギエンシス単離株がPS185L2である、請求項2記載 の抗線虫性毒素。 10.バチルス・チューリンギエンシス単離株がPS197P3である、請求項2記 載の抗線虫性毒素。 11.バチルス・チューリンギエンシス単離株がPS242B6である、請求項2記 載の抗線虫性毒素。 12.バチルス・チューリンギエンシス単離株がPS242G4である、請求項2記 載の抗線虫性毒素。 13.バチルス・チューリンギエンシス単離株がPS242H10である、請求項2記 載の抗線虫性毒素。 14.バチルス・チューリンギエンシス単離株がPS242K17である、請求項2記 載の抗線虫性毒素。 15.バチルス・チューリンギエンシス単離株がPS244A2である、請求項2記 載の抗線虫性毒素。 16.バチルス・チューリンギエンシス単離株がPS244D1である、請求項2記 載の抗線虫性毒素。 17.PS32B、PS49C、PS52E3、PS54G2、PS101CC3、PS187D4、PS185L2、PS197P 3、PS242B6、PS242G4、PS242H10、PS242K17、PS244A2、PS244D1、およびそれら の変異体からなる群より選択されるバチルス・チューリンギエンシス単離株に由 来する抗線虫性毒素、または該毒素の抗線虫性断片もしくは変異体をコードする ポリヌクレオチド配列。 18.PS32B株に由来する抗線虫性毒素をコードする、請求項17記載のポリ ヌクレオチド配列。 19.PS49C株に由来する抗線虫性毒素をコードする、請求項17記載のポリ ヌクレオチド配列。 20.PS52E3株に由来する抗線虫性毒素をコードする、請求項17記載のポリ ヌクレオチド配列。 21.PS54G2株に由来する抗線虫性毒素をコードする、請求項17記載のポリ ヌクレオチド配列。 22.PS101CC3株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 23.PS178D4株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 24.PS185L2株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 25.PS197P3株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 26.PS242B6株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 27.PS242G4株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 28.PS242H10株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 29.PS242K17株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 30.PS244A2株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 31.PS244D1株に由来する抗線虫性毒素をコードする、請求項17記載のポ リヌクレオチド配列。 32.以下の各項からなる群より選択されるプライマー対を用いてPCRにより 増幅しうる、抗線虫性毒素をコードするポリヌクレオチド配列: (a) V3-ΔV5'プライマー対、配列番号:1および配列番号:4 (b) V3-ΔV8'プライマー対、配列番号:1および配列番号:5 (c) V7-ΔV8'プライマー対、配列番号:3および配列番号:5、ならびに (d) V5-ΔV8'プライマー対、配列番号:2および配列番号:5。 33.V7-ΔV8'プライマー対、配列番号:3および配列番号:5を用いて増幅 しうる、請求項32記載のポリヌクレオチド配列。 34.増幅により約320bpの断片が産生される、請求項33記載のポリヌクレ オチド配列。 35.PS32B、PS49C、PS52E3、PS54G2、PS101CC3、PS178D4、PS185L2、PS197P 3、PS242B6、PS242G4、PS242H10、PS242K17、PS244A2、PS244D1およびその変異 体からなる群より選択されるバチルス・チューリンギエンシス単離株に由来する 抗 線虫性毒素、または該毒素の抗線虫性断片もしくは変異体をコードする、請求項 32記載のポリヌクレオチド配列。 36.配列番号:1、配列番号:2、配列番号:3、配列番号:4、配列番号 :5、および配列番号:1から配列番号:5のいずれかとハイブリダイズするポ リヌクレオチド配列からなる群より選択される単離ポリヌクレオチド配列。 37.配列番号:1である、請求項35記載の単離ポリヌクレオチド。 38.配列番号:2である、請求項35記載の単離ポリヌクレオチド。 39.配列番号:3である、請求項35記載の単離ポリヌクレオチド。 40.配列番号:4である、請求項35記載の単離ポリヌクレオチド。 41.配列番号:5である、請求項35記載の単離ポリヌクレオチド。 42.請求項32記載のポリヌクレオチド配列で形質転換された組換え宿主。 43.細菌である、請求項42記載の組換え宿主。 44.バチルス・チューリンギエンシス(Bacillus thuringiensis)、大腸菌 (Escherichia coli)およびシュードモナス(Pseudomonas)からなる群より選 択される、請求項43記載の組換え宿主。 45.植物である、請求項42記載の組換え宿主。 46.PS32B(NRRL B-21531)、PS49C(NRRL B-21532)、PS52E3(NRL B-21533)、P S54G2(NRRL B- )、PS101CC3(NRRL B-21534)、PS178D4(NRRL B- )、PS185L2( NRRL B-21535)、PS197P3(NRRL B-21536)、PS242B6(NRRL B-21537)、PS242G4(NRR L B-21538)、PS242H10(NRRL B-21539)、PS242K17(NRRL B-21540)、PS244A2(NRRL B-21541)、PS244D1(NRRL B-21542)、およびそれらの変異体からなる群より選択 される同定されている特徴を有するバチルス・チューリンギエンシス単離株に由 来する毒素、または該毒素の抗線虫性断片もしくは変異体と、線虫を接触させる ことを含む、線虫を制御する方法。 47.毒素が、86Q3(a)またはその抗線虫性断片である、請求項46記載の方 法。 48.毒素が、PS201T6であるか、またはPS201T6由来の毒素の抗線虫性断片で ある、請求項46記載の方法。 49.毒素が、約30kDaの毒素であるか、またはその抗線虫性断片である、請 求 項48記載の方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/540,104 US5667993A (en) | 1995-10-06 | 1995-10-06 | Primers and probes for the identification of bacillus thuringiensis genes and isolates |
US08/540,104 | 1995-10-06 | ||
US08/620,717 | 1996-03-21 | ||
US08/620,717 US5670365A (en) | 1995-10-06 | 1996-03-21 | Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates |
PCT/US1996/015730 WO1997012980A1 (en) | 1995-10-06 | 1996-10-01 | Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates |
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JPH11512616A true JPH11512616A (ja) | 1999-11-02 |
JP4359653B2 JP4359653B2 (ja) | 2009-11-04 |
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JP51436697A Expired - Fee Related JP4359653B2 (ja) | 1995-10-06 | 1996-10-01 | 抗線虫性バチルス・チューリンギエンシス(Bacillus thuringiensis)遺伝子、毒素、および単離株の同定およびその利用 |
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US (1) | US5670365A (ja) |
EP (1) | EP0853671B1 (ja) |
JP (1) | JP4359653B2 (ja) |
AT (1) | ATE287955T1 (ja) |
AU (1) | AU729343C (ja) |
CA (1) | CA2232233A1 (ja) |
DE (1) | DE69634258T2 (ja) |
ES (1) | ES2235198T3 (ja) |
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WO (1) | WO1997012980A1 (ja) |
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1996
- 1996-03-21 US US08/620,717 patent/US5670365A/en not_active Expired - Lifetime
- 1996-10-01 AT AT96933212T patent/ATE287955T1/de not_active IP Right Cessation
- 1996-10-01 AU AU72036/96A patent/AU729343C/en not_active Ceased
- 1996-10-01 NZ NZ319495A patent/NZ319495A/xx unknown
- 1996-10-01 ES ES96933212T patent/ES2235198T3/es not_active Expired - Lifetime
- 1996-10-01 WO PCT/US1996/015730 patent/WO1997012980A1/en active IP Right Grant
- 1996-10-01 EP EP96933212A patent/EP0853671B1/en not_active Expired - Lifetime
- 1996-10-01 CA CA002232233A patent/CA2232233A1/en not_active Abandoned
- 1996-10-01 DE DE69634258T patent/DE69634258T2/de not_active Expired - Lifetime
- 1996-10-01 JP JP51436697A patent/JP4359653B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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AU7203696A (en) | 1997-04-28 |
DE69634258D1 (de) | 2005-03-03 |
NZ319495A (en) | 1999-11-29 |
ES2235198T3 (es) | 2005-07-01 |
US5670365A (en) | 1997-09-23 |
DE69634258T2 (de) | 2005-12-22 |
JP4359653B2 (ja) | 2009-11-04 |
AU729343B2 (en) | 2001-02-01 |
EP0853671B1 (en) | 2005-01-26 |
AU729343C (en) | 2003-07-24 |
WO1997012980A1 (en) | 1997-04-10 |
EP0853671A1 (en) | 1998-07-22 |
ATE287955T1 (de) | 2005-02-15 |
CA2232233A1 (en) | 1997-04-10 |
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