JPH11349468A - Anti-sunburning agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject anti-sunburning agent that can help the prevention and recovery of the damage to skin cells due to sunburning by using an extract from pistils of saffron. SOLUTION: This anti-sunburning agent contains an extract from pistils of saffron. This extract is collected by taking out the pistils from the preliminarily sterilized and other treated floral buds of saffrons so that the styles may remain long, inoculating them to the culture medium prepared by adjusted in its pH of the Murashige and Skoog culture medium as a base and culturing the culture mixture in the dark at 25 deg.C for 32 weeks as the culture medium is exchanged every 8 weeks, then, drying the cultured tissues at 80 deg.C for 1 hour and extracting the dried tissue with 50% aqueous ethanol. This medicinal agent can be used in the preparation forms in variety, for example, lotion, cream and the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention is a sunburn-resistant agent which helps prevent and recover damage to skin cells caused by sunburn.

[0002]

2. Description of the Related Art There are various reasons why skin does not maintain a normal state, and there are various adverse effects due to ultraviolet rays. One way to prevent this is to prevent it with a hat or clothing, and it can also be prevented by applying a cream or the like containing an ultraviolet inhibitor. However, it is not possible to completely protect the skin, and there is also a merit that the ultraviolet rays hit the skin. Therefore, there is a need for a method for minimizing the adverse effects of the ultraviolet rays hitting the skin.

[0003] Saffron is a perennial herb of the Iridaceae family, Crocus sativas L. (CrocusSativus L.), whose stigma is dried is used as a crude drug. It is produced in Hiroshima, Kagawa, Oita and Iwate prefectures in Spain, France, Italy and Japan. It contains about 2% of carotenoid pigment, crocin (glycoside of crocetin) and bitter glycoside (picrocrocid).
n) about 2%, containing 0.4-1.3% of essential oils. The main component of the essential oil is safranal (fragrance component peculiar to crude drugs).

[0004] The present inventors have conducted extensive studies and found that saffron mesisibe extract helps prevent and restore damage to skin cells due to sunburn. It was found that the extraction method of saffron may be extraction with water or a hydrophilic organic solvent alone or in combination. As the hydrophilic organic solvent, ethanol, methanol, butanol, ethylene glycol, diethylene glycol, triethylene glycol, more polyethylene glycols, propylene glycol, dipropylene glycol, more polypropylene glycols, 1,3-butylene glycol, Examples thereof include butylene glycols such as 1,4-butylene glycol, glycerin, diglycerin, and higher polyglycerins.

[0005] The saffron saffron extract thus extracted can be blended into various dosage forms by removing the solvent, if necessary. For example, it can be in the form of lotions, emulsions, creams, ointments, packs, and bath preparations. In order to formulate these dosage forms, natural animal or vegetable oils such as olive oil, mink oil, castor oil, palm oil, tallow, evening primrose oil, coconut oil, castor oil, cocoa oil,
Macadamia nut oil and the like; waxes such as jojoba oil, beeswax, lanolin, carnauba wax, candelilla wax and the like;
Higher alcohols such as lauryl alcohol, stearyl alcohol, cetyl alcohol and oleyl alcohol; higher fatty acids such as lauric acid, palmitic acid, stearic acid, oleic acid, behenic acid and lanolin fatty acid; higher aliphatic hydrocarbons such as liquid paraffin; Solid paraffin, squalane, vaseline, ceresin, microcrystalline wax, etc .; synthetic ester oils such as butyl stearate, hexyl laurate, diisopropyl adipate, diisopropyl sebacate, octyldodecyl myristate, isopropyl myristate, isopropyl palmitate isopropyl myristate, Cetyl isooctanoate, neopentyl glycol dicaprate; silicone derivatives such as methyl silicone,
Silicone oil such as methylphenyl silicone

Examples of the surfactant include anionic surfactants such as alkyl sulfates, fatty acid salts, alkyl phosphates, phosphates and sulfates of polyoxyethylene alkyl ethers; nonionic surfactants such as Glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether, sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyglycerin fatty acid ester, etc .; , Phosphatidylserine, phosphatidylglycerol, phosphatidylinositol and their lyso forms, as well as phosphatidic acid and its salts

Polyhydric alcohols such as ethylene glycol, diethylene glycol, triethylene glycol,
More polyethylene glycols, propylene glycol, dipropylene glycol, more polypropylene glycols, 1,3-butylene glycol,
Butylene glycols such as 1,4-butylene glycol, glycerin, diglycerin, and more polyglycerins, sorbitol, mannitol, xylitol,
Sugar alcohols such as maltitol, ethylene oxide (hereinafter abbreviated as EO) of glycerin, propylene oxide (hereinafter abbreviated as PO) adduct, sugar alcohol E
O, PO adducts, monosaccharides such as galactose, glucose and fructose and their EO, PO adducts, maltose,
Polysaccharides such as lactose and polyhydric alcohols such as EO and PO adducts

As drugs, tocopherol, tocopherol acetate, vitamin C, allantoin, placental extract, elastin, arbutin, collagen, triclosan, trichlorocarban, dipotassium glycyrrhizinate, methylparaben, butylparaben

[0009] These ingredients are combined to obtain the required preparation. However, the surfactant is disclosed in JP-A-3-83909,
As described in JP-A-5-43416 and JP-A-6-9333, there is also one advantage by not blending, and depending on the purpose, it is not blended depending on the purpose. Of course, other raw materials are blended as needed.

[0010]

EXAMPLES The present invention will be described more specifically with reference to the following production examples and examples. However, it goes without saying that the present invention is not limited to these production examples and examples.

Production Example After removing the discolored part of the saffron bulb from which the flower buds extended to about 10 cm, and removed the discolored part, the bulb was washed with running water for 3 hours. 10
% Of benzalkonium chloride for 3 to 4 seconds, 70% of ethanol for 1 to 2 seconds, and 5% of sodium hypochlorite for 20 to 40 minutes. After that, it was performed aseptically. The flower buds were cut off except for the bulb portion and washed three times with sterile water. Next, the buds were taken out from the flower buds, cut and incised, and the mesibes were taken out in a state where the style was long and inoculated on the medium. The medium is Murashige
and Skoog's medium was adjusted to 4% saccharose, 0.9% agar powder, pH 5.8. To this was added 10 ppm of naphthalene acetic acid and 1 ppm of benzyl adenine. This was left in a dark place at 25 ° C. Change the medium every 8 weeks,
Cultured for 32 weeks. The cultured tissue was dried at 80 ° C. for 1 hour and extracted with a 50% aqueous ethanol solution.

Example 1 Lotion Olive Oil 0.5 Extract of Preparation Example 0.5 Polyoxyethylene (20E.O) Sorpitan Monostearate 2.0 Polyoxyethylene (60E.O) Hardened Castor Oil 2.0 Ethanol 10.0 1.0% aqueous solution of sodium hyaluronate 5.0 Purified water 80.0

Example-2 Cream A Squalane 20.0 Olive Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerin Monostearate 1.0 Sorbitan Monostearate 2. 0 Extract of Preparation Example 1.0 B Purified water 47.9 Polyoxyethylene (20E.O) sorbitan monostearate 2.0 Polyoxyethylene (60E.O) hydrogenated castor oil 1.0 Glycerin 5.0 0% aqueous sodium hyaluronate solution 5.0 Methyl parahydroxybenzoate 0.1 A and B were each weighed and heated to 70 ° C., and A was gradually added to B while stirring, and then slowly stirred for 30 minutes.
Cooled to ° C.

An experiment was conducted to see how these are effective. UV damage recovery ability test Test method Human normal epidermal cells (Kurabo Industries, Ltd., NHEK) are subcultured and inoculated in 35 mm Petri dishes. (2 to 50,000) 2. Culturing until reaching 60 to 60% confluence. 3. Wash the Petri dish twice with PBS (-), then add 0.5ml of PBS (-)
Added. 4. Irradiate 1.5 J / cm2 of UV light. (Strength 2.0 ~
2.5 mW / cm ² ) 5. Add 0.5 ml of the test sample solution prepared in the medium,
Incubate for 2 hours. 6. The culture solution is sampled, placed in a 1.5 ml tube, and centrifuged. The dehydrogenase activity is measured using the supernatant as free dehydrogenase. 7. The Petri dish was washed twice with PBS (-), and then 0.1% polyoxyethylene sorbitan monolaurate (20E.O.) was added to the dish.
Add ml. 8. Peel off the cells remaining in the Petri dish with a scraper.
Put in a 5ml tube. 9. Centrifuge and measure the dehydrogenase activity using the supernatant as viable cells. Ten. The recovery rate was calculated by calculating the ratio of the amount of viable cell dehydrogenase activity to the total amount of dehydrogenase activity in the petri dish, and expressed as a relative value to the control without addition.

Method for measuring dehydrogenase activity (using a dehydrogenase measurement kit manufactured by Far East Pharmaceutical Co.) 1. 0.02 PBS (-) was added to each well of a 96-well plate.
Add 5 ml. 2. A sample for measuring free dehydrogenase activity was 0.025
Add ml. 3. The supernatant for the measurement of viable cells was diluted at time to 0.025.
Add ml. 4. Add 0.05 ml of the reaction solution and incubate at 37 ° C for 30 minutes. 5. Add 0.1 ml of the reaction stop solution, and measure the absorbance at 540 nm using a plate reader. The same test was performed using human normal fibroblasts (without reference). However, a test article is prepared using a serum-free medium containing 0.1% bovine serum albumin. (All are UV-
A and UV-B)

The results of the experiment are shown in Table 1, and it was found that the damage of the cells due to the ultraviolet rays was recovered.

[Table 1]

UV damage protection ability test Test method 1. Human normal epidermal cells (NHEK manufactured by Kurabo Industries) are subcultured and planted in a 35 mm petri dish for 20,000 minutes. 2. Use a growth medium for normal epidermal cells (Kurabo KG2)
Replace with the test solution prepared in the above and culture for 5 days. (Replace every other day.) 3. Wash the Petri dish twice with PBS (-), then add 0.5ml of PBS (-).
Added. 4. Irradiate 1.5 J / cm2 of UV light. (Strength 2.0 ~
2.5 mW / cm ² ) 5. Add 0.5 ml of medium and incubate for 2 hours. 6. Sample the culture, place in a 1.5 ml tube, and centrifuge. The supernatant is used as free dehydrogenase and the dehydrogenase activity is measured. 7. Wash the Petri dish twice with PBS (-), then add 1 ml of 0.1% polyoxyethylene sorbitan monolaurate (20E.O.).
Added. 8. Peel off the cells remaining in the Petri dish with a scraper.
Put in a 5ml tube. 9. Centrifuge and measure the dehydrogenase activity using the supernatant as viable cells. 10. The protection rate was calculated by calculating the ratio of the dehydrogenase activity of the surviving cells to the total dehydrogenase activity in the petri dish, and expressed as a relative value to the control without addition. The measuring method of the dehydrogenase activity is the same as that in the ultraviolet damage recovery ability test. A similar test was performed using normal human fibroblasts. However, a test article is prepared using a serum-free medium containing 0.1% bovine serum albumin. (All are UV-
A and UV-B)

The results of the experiment are shown in Table 2, and it was found that it was effective to apply the damage of the cells by ultraviolet rays in advance.

[Table 2]

Usage Test The face of each of six women was divided into left and right, one was used as an example, and the other was used as a comparative example. The season began on July 1. In the comparative example, the extract of the production example was replaced with water from the example. (Comparative Examples 1 and 2) The following table summarizes the results of the questionnaire with the following criteria. Example is very good 3 Example is considerably better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

[0020]

[0021]

[Effects] Thus, in both cells and actual persons, it has been found that a sunburn-resistant agent containing a saffron mesibie extract helps prevent and recover damage to skin cells caused by sunburn.

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[Procedure amendment]

[Submission date] November 9, 1998

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0016

[Correction method] Change

[Correction contents]

The results of the experiment are shown in Table 1, and it was found that the damage of the cells due to the ultraviolet rays was recovered.

[Table 1]

Claims (1)

[Claims] Claims: 1. A sunburn-resistant agent containing an extract of saffron mesbeci