KR20170050062A

KR20170050062A - Composition for Beverage and Cosmetics Containing Asiaticoside Madecassoside Asiatic acid Madecasic acid Extracted from Centella Asiatica Manufacturing Method Thereof

Info

Publication number: KR20170050062A
Application number: KR1020150151106A
Authority: KR
Inventors: 최경석; 최환
Original assignee: 최경석
Priority date: 2015-10-29
Filing date: 2015-10-29
Publication date: 2017-05-11

Abstract

The present invention relates to a beverage and cosmetic composition containing asiaticoside, madecassoside, asiatic acid and madecasic acid obtained from a centrum, and a manufacturing method thereof. The invention comprises the following steps: obtaining a mixture of decolorized and concentrated solutions made with glycoside such as asiaticoside and madecassoside, by allowing the centrum to be reacted with alcohol and filtered; obtaining asiaticoside by discoloring, concentrating and crystallizing the glycoside mixture; obtaining hydrolysate such as asiatic acid and madecasic acid by allowing the mixture of the glycoside including asiaticoside and madecassoside to be dissolved by alcohol, reacted with a strong alkali and dehydrated by a strong acid; manufacturing a cosmetic or beverage composition by mixing the glycoside including asiaticoside and madecassoside, or hydrolysate such as asiatic acid and madecasic acid, which can be used in a single or in combination of two or more and mixed with cosmetic or beverage minor ingredients. The invention is capable of providing effects of moisturizing, wound healing and cell regeneration through a beverage or cosmetic composition by mixing asiaticoside and madecassoside or asiatic acid and madecasic acid in a single or in combination of two or more.

Description

TECHNICAL FIELD The present invention relates to a composition for beverage and cosmetic composition containing Asian ticoside, madecasoside, asialic acid, and methacaric acid isolated from a centrum and a method for producing the same. Manufacturing Method Thereof}

The present invention relates to a beverage and cosmetic composition comprising Asian ticoside, madecasoside, asialic acid and methacaric acid isolated from a centrum, and a method for producing the same. More particularly, the present invention relates to a method for producing a glycoside compound, which comprises reacting a fenugreek with an alcohol and filtering the mixture to thereby obtain a mixture of glycosidic compounds such as Asiaticoside and Madecassoside, Crystallizing the mixture to obtain an Asian ticoside; and dissolving the glycoside asidoside and the mixture of madecassoside in alcohol, reacting with a strong base, neutralizing with strong acid, and concentrating and drying to obtain a hydrolyzate, asiatic acid, And Madecasic acid; and a step of mixing the above glycosides such as Asian ticoside and madecassoside or hydrolyzate, asialic acid and methacaric acid, alone or in admixture of two or more with cosmetic or beverage ingredients, To form a composition or beverage composition.

The present invention is to provide a beverage composition or a cosmetic composition by using alone or in combination of an Asian ticoside and a madecassoside or an asialic acid and a madeca acid to provide effects such as moisturizing effect, wound healing and cell regeneration.

Centella asiatica is a perennial plant called the tiger grass and clam, which is common in the mountains and fields of southern islands of the peninsula. It has been widely used for skin diseases due to burns, wound healing and protection. The total amount of Asian ticoside and madecasoside is 2 ~ 8% depending on the growing area. When they are extracted with ethanol, Asian ticoside and Asian acid are obtained. Wound healing, and cell regeneration. The pharmacological mechanism is known to have an effect of promoting collagen biosynthesis in skin cells.

As a related art related to the present invention, Korean Patent Laid-Open Publication No. 10-2015-0050310 is a composition for improving skin condition containing a mixed extract of Cheongho, milt leaf, Korean green pepper and green tea or a fraction thereof as an active ingredient. Korean Patent Laid-Open No. 10-2003-0024932 is a dried product of Kunchuk extract containing 0.0001 to 10.0% by weight, based on the total dry weight of the cosmetic composition, using the active ingredient, Asian ticoside. Korean Patent No. 10-0641302 is a cosmetic composition for improving skin wrinkles containing Asian ticoside and Asian acid at a ratio of 1.5 to 2.5: 1. Korean Patent No. 10-0511944 is a cosmetic composition comprising a multilayer liposome in which Asian ticoside as an active ingredient for improving wrinkles is captured. These prior arts have focused on moisturizing effect or wrinkle-improving effect as a composition of a cosmetic composition which utilizes a mixture of Asian ticoside alone or with an asialic acid. However, the present invention relates to a cosmetic composition containing a further addition of the compound of the formula, that is, a cosmetic cream, a shampoo, a soap, etc. to improve skin wounds and improve the cell regeneration effect. Focused on improved differentiated effects.

The present invention also provides a functional drink using a single ingredient or a mixture of Asian ticoside, madecaside, asialic acid, and methacaric acid, and has an anti-epileptic function utilizing cell regeneration effect. Until now, no patent has been developed as a functional drink And the present invention is unique.

The chemical structures of the Asian ticoside, the madecaside, the asialic acid and the methacaric acid mentioned in the present invention are as shown in the following chemical formulas (1) to (4). The following formula 1 is an asymmetric acid, the following formula 1 is asiaticoside, the following formula 2 is a madecosaide, the following formula 3 is an asialic acid, and the following formula 4 is a magetic acid.

Korean Patent Registration No. 10-0641302

It is an object of the present invention to provide a cosmetic composition that is more effective for skin moisturizing and wrinkle-improving as well as wound healing and cell regeneration. The present invention also provides a functional beverage having wound healing and cell regeneration effects, as well as a skin improving effect, by using a centilla extract or a hydrolyzate thereof singly or in combination.

In the prior art, when extracts of the clover are extracted with ethanol, only the Asian ticoside and the Asian acid are obtained, and they have been used as a cosmetic composition for moisturizing or improving wrinkles by using them alone or in a mixture. However, since there is no technique for separating madecasoside and methacaric acid separately, the effect of improving skin beauty is deteriorated because they are not included, and consumers who do not use such cosmetics have a disadvantage that it is difficult to expect a skin cosmetic improvement effect of centilla extract.

The present invention relates to a method for producing an Asian ticoside, which comprises reacting a centromeric clove with an alcohol and filtering the mixture to obtain a mixture thereof by decolorizing and concentrating the glycoside, Asian ticoside and a magicacosaide solution, and then crystallizing the glycoside mixture, Dissolving the glycoside asialicoside and the madecaside mixture in an alcohol and reacting the mixture with a strong base; neutralizing with a strong acid, followed by concentration and drying to obtain hydrolysates asialic acid and methacaric acid; And a step of mixing a cosmetic or a beverage with a combination of at least one of glycyrrhizic acid, asialicoside, madecassoside or hydrolysates, asialic acid and methacaric acid, to form a cosmetic composition or a beverage composition.

1) Extract of glycosides Asian ticoside and Madecasoside

Alcohols are added to the pulverized seedlings, which are then heated, stirred, filtered, decolorized with activated charcoal, and concentrated. The resulting solid is cooled, filtered and dried to obtain a mixture of glycoside, aspartic acid and madecaxone.

2) Crystallization of Asian ticoside sole material

The mixture of glycosides Asian ticoside and madecasoside was filtered, discolored, concentrated and crystallized, and the resulting solid was filtered and dried to obtain an Asian ticoside.

3) Crystallization of hydrolysates, asialic acid and methacaric acid

The mixture of Asian ticoside and madecasoides of glycosides is dissolved in alcohol and added with strong bases (NaOH, KOH, etc.) and adjusted to pH 5 ~ 7 with strong acid (HCl, H ₂ SO ₄ etc.) A solid of the madecanoic acid is produced.

4) Used for cosmetics and beverages of ginseng gypsum and hydrolyzate

The present invention provides a cosmetic composition having an effect of wound healing and cell regeneration by obtaining a mixture of an Asian ticoside and a madecasoide, a mixture of an Asian ticoside and a saccharide, and an Asian ticoside and a hydrolyzate of a centipede, By applying a mixture of two or more paper to a functional beverage, it can be used for skin improvement and skin disease prevention and treatment in daily life. This suggests that the conventional cosmetic composition is more effective than the moisturizing or wrinkle-improving application of centilla extracts. That is, 30 to 50% (w / w) of Asian ticoside was added to two kinds of mixtures containing 30 to 50% (w / w) of asialic acid and 30 to 50% (w / w) , Or a mixture of two kinds of asialicoside and madecassoside.

The content of the asialicoside and the madecosaide used in the present invention is 50% (w / w) or more, 50% (w / w) or more, respectively, (W / w) according to cosmetic creams, lotions, shampoos, soaps, functional beverages and the like used in the preparation of the composition.

The conventional cosmetic composition using a mixture of Asian ticoside alone or with an asialic acid shows only moisturizing effect and wrinkle-improving effect. However, the present invention can provide a cosmetic or beverage composition by adding madecaside, asialic acid or methacaric acid to the Asian ticoside to provide wound healing, cell regeneration and skin disease prevention effects.

1 is a manufacturing process diagram of the present invention.

In the present invention, Asian ticoside, madecaside, and asialic acid and methacaric acid to be used by the present invention were produced from the centellae by the following method.

<First step>: Asian Ticoside And Madecasso Side extraction.

1 kg of the dried centipede is pulverized, and 3 to 10 kg of ethanol is added at 3 to 10 kg (w / w) at 40 to 60 ° C, followed by agitation for 8 to 12 hours to extract the mixture of the Asian ticoside and the madecosaide. The extract was filtered, decolorized with activated charcoal, and then the ethanol was concentrated to 80%. The resulting solid was cooled to 24 to 26 ° C, filtered and dried to obtain 68 g of asialicoside and madecassoside. As the extraction solvent, in addition to ethanol, lower alcohol such as methanol, propanol, and butanol may be used.

<Second Step>: Asian Ticoside Single material crystallization

In the first step, the filtered filtrate was decolorized and concentrated to crystallize. The resulting solid was filtered and dried to obtain 30 g of Asian ticoside.

<Step 3>: Asian Acid Mountain Of Madeca crystallization

After dissolving 6 L of 70% (w / w) ethanol obtained in the first step and 120 mL of 20% NaOH as a hydrolyzing agent, stirring was carried out at 70-80 ° C for 3-4 hours, (W / w) HCl to adjust the pH to 5-7 and concentrate to remove the ethanol, resulting in the formation of asialic acid and methacaric acid. After filtration and drying, 41 g of a mixture of at least 80% (w / w) of asyric acid and methacaric acid was obtained. In this case, KOH may be used instead of NaOH as the hydrolyzing agent, and H ₂ SO ₄ may be used instead of HCl as a neutralizing agent.

< Asian Ticoside Analysis conditions>

After dissolving 20 mg of methanol in 50 ml of Asian ticoside and using a C18, 3.9 x 150 mm (Bondapak) column, the ratio of acetonitrile: methanol: water = 10: 40: In the squad, Asian ticoside and Madecaso side appear in 1.1 minutes.

< Asian Acid Mountain Of Madeca Analysis conditions>

Methanol (50 ml) was dissolved in 30 mg of the two components, and then a C8, 4.6 × 150 mm (Beckman) column was applied at a flow rate of 1.5 ml / min in a ratio of acetonitrile: methanol: water = 3: 3: , And peak of asialic acid appeared in 8.7 minutes. Asiatic acid was 40.2% and that of methacaric acid was 44.1%. The sum of both substances was 84.3%, which satisfied 50% (w / w) or more.

Thus, in the present invention, 41 g of the obtained mixture of asialic acid and methacaric acid and 30 g of Asian ticoside were mixed in the following proportions to prepare a composition to be added to the final cosmetic as shown in Table 1.

ingredient Weight ratio (%) Weight (g) Acetic acid and methacrolein 57.6 41 Asian Ticoside 42.4 30 system 100% 71

The three-component composition obtained from the present invention can be used in an amount of 0.01 to 10% (w / w) based on the total weight of cosmetic and functional beverages, preferably 0.2 to 0.5% (w / w). In addition, the centipede composition of the present invention (a mixture of Asian ticoside, madecasoside, asialic acid, and methacaric acid) can be used for wound healing medicines, intrinsic medicinal food additives and the like for oral use. Especially when it is added to beverages and so on, it is possible to cure the inner ear such as the stomach due to the wound healing function. Cosmetics can be widely used such as nutritional cream, softening longevity, skin adhesive patch, essence, nutritional gel, massage cream, pack, soap, water bottle, shampoo, etc. Especially when it is used for cosmetics, purified water, oil, surfactant , A moisturizing agent, a high alcohol, a pigment, a fatty acid, a chelating agent, an antiseptic, a wax, an antioxidant, a fragrance, a pH adjusting agent and the like.

Among the above excipients, as the oil component, mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, dimethicone, Pentasiloxane, sunflower seed oil and the like are used. Surfactants include, but are not limited to, sorbitan sesquiunorate, polysorbate 60, glyceryl stearate, prochymal glyceryl stearate, sorbitan oleate, sorbitan stearate, diai-cetyl phosphate, sorbitan stearate / Croscocite, glyceryl stearate / polyethylene glycol-100 stearate, cetearyl-6 olivate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil and the like. As the moisturizing agent, glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin, betaine, glycereth-26, methylglucose-20 and the like are used. As the higher alcohol, cetyl alcohol Stearyl alcohol, octyldodecanol, isostearyl alcohol and the like are used. Examples of thickening agents include xanthan gum, carbomer, magnesium aluminum silicate, cellulose gum and dextrin palmitate. Examples of the chelating agent include disodium, Tetra sodium dithiophosphate and the like are used. As the fatty acid, stearic acid, myristic acid, palmitic acid, isostearic acid, lauric acid and the like are used, and as the fatty acid, Examples of the preservative include methylparaben, butylparaben, propylparaben, phenoxyethanol, imidazolidinyl urea, chlorphenacin and the like as the preservative. As the wax, for example, Carnauba wax, beeswax, beeswax, etc. may be used. As the pH adjusting agent, triethanolamine, citric acid and the like may be used. As the fragrance, natural fragrance, combination fragrance and the like may be used.

Thus, in a preferred embodiment, the present invention relates to a cosmetic wrinkle containing the above composition. In a more preferred embodiment, the wrinkle-improving cosmetic product includes a nutritive cream, a convergent lotion, a soft lotion, an essence, a nutritional gel, and a massage cream.

The present invention also relates to a cosmetic composition for the purpose of additionally using madecasone or methacaric acid, which enhances wound healing and cell regeneration function, which is not solely or in combination with Asian ticoside and asialic acid of the existing patent in Table 2 do.

As described above, the present invention is also applicable to a mixture of asialic acid and methacaric acid, which can be obtained by hydrolysis of asialicoside and madecassoside.

These compounds, which are the components of the centipedes, are not toxic, and they are also sold as gastrointestinal drugs because of their ability to heal and regenerate cells. Since they can be developed as gastrointestinal diseases or other non-pharmaceutical beverages, Were used alone as shown in Table 2 to prepare health functional beverages.

Centipede extract ingredient One Asian ticoside single component 2 Mixed ingredients of Asian ticoside and Asian acid 3 Asian Ticoside and Madecasoide Mixed Ingredients 4 Mixture of asialic acid and methacaric acid 5 Asian ticoside, asialic acid, mixed with methacrolein acid

When preparing a functional drink with the composition as shown in Table 2, it is aimed to enhance the function of the beverage composition by adding the components of the centilla extract 1, 2, 3, 4 or 5 to the generally applied beverage composition.

In general, sweeteners to be used in beverages include sardine glucose, maltose, fructose, stevioside isomer, honey, aspartame, acesulfame K and the like. Examples of the organic acid include citric acid, lactic acid, various fruit extracts, Natural ginseng extracts such as wood, dandelion, red ginseng, jujube, squid, and ginger are used. Gellan gum, guar gum, alginic acid sodium, CMC sodium and the like are used as the viscosity regulators, and sodium benzoate, P-metal benzoic acid, Propyl benzoic acid and the like can be used. D-sorbitol and xylitol can be used as polyhydric alcohols, citric acid, vitamin C, taurine, royal jelly and oligosaccharide can be added in various forms. To provide a health functional beverage.

< Example 1> Nourishing Cream 1

The mixture ratio of nutritional cream was 0.2% (w / w) of mixture of Asian ticoside and madecasoside as a centipede extract ingredient. The nutritional cream composition is composed of 6% w / w 1,3-butylene glycol, 46% w / w glycerin, 1.26% w / w mineral oil, 36% w / w polysorbate 60, Nutrition was performed using purified water of 56% (w / w) cetyl alcohol, 0.16% (w / w) propylparaben, benzophenone-9 26% (w / w), carboxyvinyl polymer 16% Cream.

< Example 2> Milk lotion

Mixing ratio of milk lotion in cosmetic ingredients was 0.2% (w / w) of mixture of Asian ticoside and didecanoic acid as herbal extract ingredients. The milk lotion composition contains propylene glycol 6% (w / w), mineral oil 4% (w / w), triethanolamine 1.2% (w / w), tocopheryl acetate 3% (w / 5% (w / w), allantoin 3% (w / w), macadamia nut oil 2% (w / w), polysorbate 20 1.5% (w / w), sorbitan sesquioleate 1% (w / w), 0.1% (w / w) propylparaben and 1% (w / w) lecithin.

< Example 3> Nutrition Cream 2

Among the cosmetics, the centipede extract of the nutritional cream was used as a mixture of 0.5% (w / w) of an Asian ticoside and a madecassoside. The composition of the nutritional cream is composed of 0.2% (w / w) alanantonin, 7% (w / w) 1,3-butylene glycol, 0.1% w / w tocopheryl acetate, 2.2% w / w stearic acid, (w / w), ceramide 2% (w / w), polysorbate-60 2% (w / w), sorbitan sesquioleate 2.5% (w / ), Carboxyvinyl polymer 1% (w / w), mineral oil 4% (w / w), vegetable oil 4% (w / w), sodium hyaluronate 0.5% (w / w / w) and the rest of the purified water.

< Example 4> Massage Cream

Among the cosmetics, 0.3% (w / w) of a mixture of Asian ticoside, asialic acid and methacaric acid was used as a centipede extract component of massage cream. The massage cream composition comprises at least one composition selected from the group consisting of: 6% w / w 1,3-butylene glycol, 4% w / w glycerin, 0.5% w / w triethanolamine, 0.1% w / w tocopheryl acetate, (W / w), 2% w / w cetearyl alcohol, 2% w / w alentonine, 2.1% w / w polysorbate-60, sorbitan sesquioleate 2.5 A massaging cream was prepared using purified water of% w / w, 0.1% w / w propylparaben, 1% w / w carboxyvinyl polymer, 0.2% w / w sodium hyaluronate, .

< Example 5> Cosmetics pack

Among the cosmetics, 0.2% (w / w) of a mixture of Asian ticoside and a madecasonic acid was used as a centipede extract component of the pack. The pack composition contained 3% w / w ethyl alcohol, 0.02% w / w EDTA-2Na, 5.1% w / w propylene glycol, 4.5% w / w glycerin, 1% w / w), 0.2% (w / w) of polyoxide, 0.2% (w / w) of sodium hyaluronate and the rest of purified water.

< Example 6> Body soap soap

Among the cosmetics, 0.2% (w / w) of a mixture of Asian ticoside, asialic acid, and methacaric acid was used as a soap extract for body soap. The body soap composition comprises 20% (w / w) of Coconut Oil, 30% (w / w) of Olive Oil, 20% (w / w) of Evening Oil, Palm Oil 4% w / w Avocado Oil 10% w / w NaOH 9% w / w purified water 6% w / w Other Spices 0.8 % (w / w) was used to make body soap.

< Example 7> Soap for scalp

Of the cosmetic products, the extract of the soap of the scalp was 0.5% (w / w) of a mixture of Asian ticoside, asialic acid and methacaric acid. The soap composition for the scalp is composed of 30% (w / w) of Coconut Oil, 30% (w / w) of Evening Oil, 10% (w / w) of Avocado Oil, Soaps were prepared using 20% (w / w) of Palm Oil, 4% (w / w) of Seaweed Extract and 0.4% (w / w) of other fragrances.

Example 8 Shampoo composition

Among the cosmetics, 0.5 g of a mixture of Asian ticoside and madecassoside was used as a centipede extract component of the shampoo composition. The shampoo composition was prepared by mixing 100 ml of purified water, 5 g of propylene glycol, 1 g of glycerin, 8 g of sodium lauryl sulfate, 5 g of cocamide dieye, 5 g of lauraamide dihey, 5 g of cocamidopropyl methacrylate, 0.5 g of tocopherol, 0.1 g of sodium citrate, g and fragrance dose were used to prepare the shampoo.

The centilla cicada extract used in the cosmetic composition is composed of composition 1 (a single ingredient of an Asian ticoside), a composition 2 (a mixture of an Asian ticoside and an asialic acid), a composition 3 (a mixture of an Asian ticoside and a madacalcoside) Mixture of acid and methacaric acid), Composition 5 (mixture of Asian ticoside and asialic acid, mixed with methacrolein) was used. In addition, cosmetic composition was tested for skin regeneration and wound healing satisfaction test, skin cell regeneration effect, wound healing effect, skin elasticity improvement effect test and wrinkle improvement effect by using components of nutritional cream blend ratio.

< Test Example 1>; Skin regeneration and wound healing satisfaction survey

Skin regeneration and wound healing satisfaction were investigated according to the blending ratio of the above compositions 1 to 5. 50 females and 60 males and females who received laser treatment and peeling treatment were applied to the right side of the same face and the left side of the same face to the treatment site, respectively. And applied twice a day (morning / evening) for 1 week. The degree of satisfaction with the degree of skin regeneration and wound healing improvement was examined and the results are shown in Table 3 below.

division Very good Very good good usually lack persons % persons % persons % persons % persons % Test Example 1 Composition 1 2 20 3 30 5 20 One 10 - - Control group - - - - 6 60 3 30 One 10 Test Example 2 Composition 2 3 30 3 30 2 20 2 20 - - Control group - - One 10 6 60 2 20 One 10 Test Example 3 Composition 3 5 50 3 30 One 10 - - - - Control group - - - - 5 50 4 40 One 10 Test Example 4 Composition 4 4 40 3 30 3 30 - - - - Control group - - - - 4 40 4 40 2 20 Test Example 5 Composition 5 3 30 4 40 3 30 - - - - Control group - - One 10 One 10 6 60 2 20

From the above results, it was found that Test Example 3 was very good at 50% and Test Example 4 was very good at 40%, while each control group was very good and not very good.

Therefore, the composition of the present invention is judged to have excellent skin regeneration and wound healing satisfaction.

< Test Example 2>; Regenerative effect of skin cells

In order to examine the skin regeneration effect through the proliferation of skin cells donated to human skin fibroblasts, analysis was performed using Cell counting kit-8. And cultured in Dulbecco's Modification of Eagle's Medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin at 37 ° C and 5% CO 2. 1,000 human dermal fibroblasts were plated in a 96-well plate, and the composition of Table 4 was each treated in a cell culture medium. After 72 hours, the degree of cell proliferation was confirmed. For the measurement, the CCK-8 solution was added to the cell culture medium (100 μl / well), incubated for 3 hours in an incubator, and absorbance was measured at 450 nm. The increase in the number of cells was investigated based on the results of the standard curve. The results are shown in Table 4 below.

division Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Control group 1 time 5219 6327 7346 7299 6488 3689 Episode 2 5216 6331 7412 7312 6578 3567

From the above results, it can be seen that the composition of the present invention is superior to the control group in promoting skin regeneration. In particular, it can be seen that the composition 3 and the composition 4 have a skin regeneration promoting effect of 50% or more as compared with the dodo group.

< Test Example 3>; Wound healing effect

The inhibition of IL-6 expression in Human Epidermal Neonatal Keratinocyte Cells was confirmed by the real time-reverse transcription polymerase chain reaction (Q-RT-PCR). Human dermal keratinocytes were purchased from Lonza, Inc. (Walkersville, MD, USA). Cell culture media were subcultured in a CO ₂ incubator (CO ₂ incubator) at 37 ° C Cells were cultured under the condition of 5% CO _2. To a 500 ml KBM-2 (Clonetics CC-3103) medium was added a KGM-2 bullet kit (BPE: Bovine pituitary extract (2 ml) (hEGF: human epidermal growth factor: 0.5 mL), insulin (0.5 mL), hydrocortisone (0.5 mL), transferrin (0.5 mL), epinephrine (0.5 mL), gentamicin sulfate The human keratinocytes were filled with 70% of the surface of the cell culture plate and cultured in a medium supplemented with interferon-gamma (Glucomycin Suflate Amphofericin-B: GA-1000, 0.5 ml) 100 U / ml), and after 30 minutes, the compositions 1 to 5 of the present invention and the control group After 24 hours of treatment with each composition, the cells were treated with 10 ml of phosphate buffered saline, and the cells were incubated for 24 hours. (Trizol reagent, Invitrogen, Carlsbad, Calif., USA) to separate total RNA (total RNA). The isolated RNA was purified using RNA kit (Qiagen RNeasy kit, Qiagen, Valencia, CA CDNA was synthesized from the RNA isolated from the above using a reverse kit (Superscript Reverse Transcriptase (RT) II kit, Invitrogen, Carlsbad, Calif.), And the cDNA was synthesized by Q-RT-PCR The expression of IL-6 was quantitatively analyzed. The expression pattern of IL-6, an inflammation inducer, was evaluated using a TaqMan gene expression assay kit (Applied Biosystems, Foster City, Calif.). IL-6 gene expression analysis was performed using the non- The results are shown in Table 5 below. The inhibition rate is shown in Table 5, and the unit is%.

division Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Control group 1 time 59.2 61.1 66.3 66.7 61.4 26.8 Episode 2 60.1 61.3 65.9 66.9 61.8 28.1

From the above results, it can be seen that the composition of the present invention is superior in the inflammatory reaction inhibitory effect as compared with the control group. In particular, it can be seen that composition 3 and composition 4 have a wound healing effect of 50% or more as compared with the dodo group. As described above, the composition of the present invention is applied as a wound dressing cosmetic or beverage because it has a clear effect compared with the control group from the results of skin regeneration and wound healing satisfaction test, skin cell regeneration test and wound healing effect test have.

< Test Example 4>; Skin elasticity improvement test

The effect of improving the skin elasticity of the control group without addition of the Asian ticoside ingredient in the preparation of the nutritional cream, milk lotion, massage cream and pack of the examples was measured through clinical studies. The skin elasticity improvement effect was evaluated by measuring changes in skin elasticity. Skin elasticity was measured in 40 healthy women in their twenties, twenties, thirties, and 40s who remained constant at 24 ~ 26 ℃ and humidity 38 ~ 40%. Nutritional cream, milk lotion, massage cream and pack were applied to the subject's face twice a day for 3 months, and then measured using Cutometer SEM 474. The results are shown in Table 6 below. The results are shown in Table 6. The results are shown in Table 6 below.

division Nourishing cream Milk lotion Massage Cream pack skin
elasticity Example Control group Example Control group Example Control group Example Control group 4.25 3.72 4.21 3.85 4.12 3.75 4.21 3.83

From the above results, it can be seen that the composition of the present invention is superior to the control group in improving the skin wrinkle (elasticity) even when applied to cosmetics.

< Test Example 5>; Wrinkle improvement effect

The effect of improving the wrinkles of Example 1 (nutritional cream), Example 2 (milk lotion), Example 4 (massage cream), Example 5 (pack) and the control group was evaluated as follows.

Twenty women in their thirties and twenties were applied twice daily morning and evening for four weeks to the inside of the arm hump, and the state of the wrinkles was measured by an image analyzer Skin touch system (STS, Korea) . This value indicates the number of wrinkles is high and the number of wrinkles is six minutes. The higher the number, the more wrinkles.

division Nourishing cream Milk lotion Massage Cream pack wrinkle
Improving Example Control group Example Control group Example Control group Example Control group 1.8 3.4 1.9 3.3 1.6 3.2 1.5 3.1

From the above results, it can be seen that the composition of the present invention is superior to the control group in improving wrinkles even when applied to cosmetics.

< Example 9> Beverage composition

Five kinds of beverages were prepared using 0.2-0.6g of the centipede extract ingredients of the beverage composition as the following five cases. The composition used was 4 ml of honey, 2 ml of chito oligosaccharide, 1 g of sodium citrate, 1 g of sodium alginate, 1 g of D-sorbitol, 0.1 ml of ginseng, 0.5 ml of red ginseng concentrate, 0.2 g of vitamin C and purified water.

< Test Example 6>; Sensory test

According to the compounding ratio of the centilla extract (composition 1: 0.2, composition 2: 0.3, composition 4: 0.4, composition 5: 0.45), centilla's vitamin drink (K pharmaceutical product) , And palatability were compared. (5, good, 4, moderate; 3, insufficient; 2 points or less) on well-trained panels (20s, 30s, 2s in each 40s).

division Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Control group smell 4.3 4.4 4.5 4.5 4.4 4.5 flavor 4.2 4.3 4.4 4.4 4.3 4.5 Likelihood 4.3 4.3 4.5 4.4 4.4 4.4

From the above results, it can be seen that the beverage composition using the Centella asiatica extract retains the same or similar range of taste even when the products are compared with the sensory properties.

The screen fold extract of the present invention provides moisturizing effect, wrinkle improvement, wound healing and anti-epileptic effect when it is used as a beverage or a cosmetic, so that it is industrially applicable.

Claims

Cosmetic and beverage composition of wound healing, cell regeneration and skin disease prevention effect including Asian ticoside and asialic acid isolated from Centella asiatica extract

The cosmetic and beverage composition according to claim 1, which further comprises wound healing, cell regeneration and skin disease prevention, wherein the composition further comprises madecaside or methacrolein

The method according to claim 1, wherein the composition comprises 0.01 to 1% (w / w) of Asian ticoside, asialic acid, madecasoide or methacaric acid isolated from Centella asiatica Effect cosmetic and beverage composition

4. The cosmetic composition according to any one of claims 1 to 3, which is a cosmetic composition selected from the group consisting of lotions, creams, packs, sunscreens, soaps, and shampoos. And beverage composition

The cosmetic and beverage composition according to any one of claims 1 to 3, which is a beverage composition, for wound healing, cell regeneration and skin disease prevention

Reacting the mixture with alcohol and filtering the mixture to obtain a mixture thereof; and a step of discoloring and concentrating the mixture to obtain an Asian ticoside glycoside; and Dissolving a mixture of glycosides, Asian ticoside and madecasone in alcohol, reacting with a strong base, neutralizing with strong acid, followed by concentration and drying to obtain hydrolysates asialic acid and methacaric acid, And at least one of at least one of at least one of at least one of acidic acid and at least one of at least one of at least one of at least one of acidic acid and at least one acidic acid, A cosmetic for preventing skin disease and a method for producing a beverage composition