JPH11295306A - Test materials for urine inspection and health checking material using the same and method for checking health - Google Patents

Abstract

PROBLEM TO BE SOLVED: To increase water-retaining capability, and to maintain the hue of an urine inspection medicine by impregnating a high-water-absorption material having a specific absorption amount of water with one kind of the urine inspection medicines being selected from a plurality of kinds of urine inspection chemical. SOLUTION: By the high-water absorption material, it is meant that the amount of water absorption per 10 g is 15-500 g. When a hydrophilic materials is used, the absorption property of urine and urine test chemical is increased, and retention capability can be improved, thus maintaining the hue of the urine test chemical due to the urine for a long time and enabling the urine inspection result to be verified even after a long time. As the raw material of the high-absorption material, cellulose, a cellulose derivative, chitosan, or the like can be used. The inspection chemical is a reagent which detects the pH, gluecose protein, occult bleeding, ketone body, specific gravity, urea, or the like of urine and detects them, according to the change of the hue. An arbitrary method such as coating, immersion, dipping, and chemical combination treatment can be adopted to incorporate the urine inspection chemical into the high-absorption material. A material for the urine inspection is mixed with an excreta treatment material for spraying to a pet toilet or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a test material for urinalysis, a health check material and a health check method using the test material.

[0002]

2. Description of the Related Art In general, health conditions such as detection and diagnosis of pet diseases such as dogs and cats are determined by observing the condition of the animals and conducting urinalysis and blood tests. Operation is necessary, so it is impossible in general households, and can only be performed in animal hospitals. As a method that can be performed at home, there is a method that can perform a urine test without performing a urine collection operation, that is, since it is difficult to collect urine from a pet, a test paper impregnated with a urine test agent is placed in a pet nest or the like. A possible method would be to place it and then wait for the pet to urinate.

[0003]

However, if the time elapses after the pet urinates on the test paper, precipitation of salts in the urine, an increase in bacteria, etc. occur, which is not hygienic and is not preferable. In addition, when urine dries with the passage of time, the color of the urine test agent may fade, and it may be difficult to determine the test.

Further, in addition to the test paper impregnated with the urine test agent, an ink composition containing a urine test indicator is printed,
A coated or impregnated pulp fiber is known (JP-A-5-223812). Since the urine test test material uses pulp fiber as a support, it can be made to have the same size and shape as the pet excrement disposal material, and can be used in combination with the pet excrement disposal material. For this reason, it has the feature that urine tests and the like can be easily performed.

[0005] However, since the urine test material has only the water absorbency of ordinary pulp, the water retention capacity is not sufficient, and urine dries over time and the color of the urine test agent fades. In some cases, it is difficult to determine the inspection. In addition, there has been no method for easily performing various urine tests simultaneously with a single test.

[0006] Therefore, an object of the present invention is to improve the water retention capacity and maintain the color of a urine test drug by using a support material having high water absorption. Another object of the present invention is to establish a method for checking animal health in which various urine tests are performed simultaneously.

[0007]

In order to solve the above problems, a urine test material according to the present invention contains a urine test agent in a highly absorbent material having a water absorption of 15 g to 500 g per 10 g. Let me do it.

[0008] Further, the superabsorbent material can be porous cellulose particles.

The use of a highly absorbent material having a high water absorption allows the absorbed urine to be retained for a long time. Therefore, the hue of the urine test agent can be maintained for a long time.

[0010] The health check material according to the present invention comprises a plurality of types of urine test materials having different shapes or different sizes. For each urine test material having a different size, one different from a test agent selected from a pH test agent, a glucose test agent, a protein test agent, an occult blood test agent, a ketone body test agent, a specific gravity test agent, or a urea test agent It is impregnated with various test agents.

In this health check material, a urine test material having a specific shape and size is impregnated with a specific test agent, that is, a urine test material having a specified shape and size is tested. Since the medicine is one-on-one, when urine of animals such as pets is applied to this health check material,
By observing the change in the hue of the test agent and at the same time, observing the shape and size of the test material for urinalysis, it is possible to judge the measurement items and the measurement results. Since this health check material is impregnated with a plurality of types of test drugs for each urine test material having each shape and size, the health check material is sprayed on a predetermined place such as a pet toilet where animals urinate. By doing so, it is possible to perform a urine test on the test drug contained in the health check material and check the health of animals such as pets in a single test.

[0012]

Embodiments of the present invention will be described below with reference to the drawings.

The urine test material according to the present invention comprises a highly absorbent water-absorbing material containing a urine test agent.

The superabsorbent material is a material having a water absorption of 15 g to 500 g per 10 g. Among these, when a material having hydrophilicity is used, the adsorbability of urine to be absorbed and the urine test agent is enhanced, and the ability to retain these can be improved. When the ability to hold urine is improved, the hue of the urine test drug due to urine is retained for a long time in the superabsorbent material, and a urinalysis can be confirmed even after a long time has passed. If the amount of water absorption is less than 15 g, the amount of urine and urine test drug absorbed will not be sufficient, and it will be difficult to change the hue by the urine test drug more clearly, making it difficult to judge a urine test based on hue. In addition, after a long time, urine dries and the color of the urine test drug fades, and urinalysis tends to be difficult. On the other hand, those having a water absorption of more than 500 g have too high water absorption and tend to partially develop color, which may be overlooked.

Examples of the raw material of the superabsorbent material having the above-mentioned water absorption include cellulose, cellulose derivatives, chitosan, polyvinyl alcohol, hydrophilic polyurethane, hydrophilized polyolefin and the like. Among them, biodegradable raw materials such as cellulose and chitosan are more preferable in terms of disposability. Examples of the superabsorbent material include porous particles, paper, nonwoven fabric, woven fabric, and porous film using the above raw materials. The shape is not particularly limited, and may be any shape such as powder, particle, sheet such as film or sheet, and sponge, depending on the method of use and purpose of use. Specific examples of the superabsorbent material include porous cellulose particles, pulp balls, cellulose sponge, rayon nonwoven fabric, superabsorbent paper, chitosan fiber, chitosan nonwoven fabric and the like.

The above urinalysis reagent is a reagent for detecting urine pH, glucose, protein, occult blood, ketone bodies, specific gravity, urea, etc., and any reagent that detects a change in hue can be used in the present invention.

Examples of the above pH test agents include those having a pH indicator as a main component. Examples of the pH indicator include bromothymol blue (BTB) and methyl red. BTB is yellow at pH 6 or lower, but changes from yellow-green to blue (pH 7.
The hue changes in 6).

Examples of the above glucose test agents include those utilizing the reaction of glucose by an enzymatic reaction.
For example, the enzyme group of glucose oxidase and peroxidase is a combination of 4-aminoantipyrine and chlorophenol, and the enzyme group is a combination of 4-aminoantipyrine and naphtholdisulfonic acid. In the case where 4-aminoantipyrine and chlorophenol are combined with the above enzyme group, the hue changes to pink due to the presence of glucose. Further, the combination of the above enzyme group with 4-aminoantipyrine and naphthol disulfonic acid changes its hue to purple due to the presence of glucose. Further, o-tolidine can also be used in combination with the above enzymes. In this case, the hue changes to green due to the presence of glucose.

Examples of the above-mentioned protein test agents include those mainly containing an indicator for determining the presence or absence of a protein by a protein error method. Examples of the indicator include tetrabromo blue, tetrabromo thymol blue, tetrabromophenol blue and the like. Tetrabromoblue forms a complex when a protein is present, and its hue changes from an acidic yellow color to a basic blue color.

Examples of the above occult blood test agents include those containing an oxidizable indicator, an organic peroxide or the like as a main component. Examples of oxidizable indicators include o-tolidine and benzidine, and examples of organic peroxides include cumene hydroperoxide. When occult blood is present in urine, the occult blood combines with, for example, cumene hydroperoxide to generate nascent oxygen, which is
-The indicator develops color in response to trizine, benzidine, etc.

Examples of the above ketone body test agents include a combination of nickel chloride and sodium nitroprussed, and the color thereof changes from pale yellow to reddish purple in the presence of acetoacetic acid.

The above urea test agents include urease, p
A combination of H indicators can be considered, and urea reacts with the enzyme to produce ammonia, changing the pH and changing the hue.

In addition to the above urinalysis drugs, any drug for test papers can be used.

As a method of incorporating the urine test agent into the highly absorbent material, any method such as coating, impregnation, dipping, and chemical bonding can be employed.

When a hydrophilic resin is used as the high-absorbency material and the urine test agent is a hydrophilic substance, both of them have hydrophilic properties. Can be easily contained. In addition, when a hydrophilic resin is used as the high-absorbency material, and the urine test agent is a hydrophobic substance, a surfactant or the like is added to the urine test agent and emulsified in water. The affinity for the material is improved, and the urine test agent can be easily contained by impregnating the highly absorbent material with the urine test agent.

The obtained urine test material can be mixed with excrement disposal materials and the like and dispersed to the urination area of animals such as pet toilets by modifying the shape of the urine test material, thereby facilitating urinalysis. It can be carried out. Further, since a sufficient amount of urine can be held for a long time, the hue of the urine test drug is held for a long time, and the necessity of frequently checking the hue of the urine test drug is eliminated.

Further, when several urine test materials impregnated with several test agents are mixed and used simultaneously, a plurality of urine tests can be performed at the same time, and various test data can be simultaneously obtained. Obtainable.

Next, a description will be given of a health check material capable of simultaneously performing a plurality of types of urine tests and simultaneously obtaining various test data by using the above urine test materials.

The health check material according to the present invention comprises a plurality of types of urine test materials having different shapes or different sizes. Among the plurality of urine test materials, the above-mentioned pH test agent, glucose test agent, protein test agent, occult blood test agent, ketone test agent , A specific gravity test agent, or a different test agent selected from urea test agents. This will be described using a specific example. FIG.
As shown in (1), a plurality of urine test materials having different shapes and sizes are prepared. In addition, among the above test drugs, test drugs A to F to be used for the test are prepared. A urine test material having one specific shape and size is impregnated with one test agent. A urine test material having a specific shape and size different from the above is impregnated with one different test agent. In this way, the test is performed on urine test materials having different specific shapes and sizes. This allows
The shape and size of the test agents A to F impregnated into the urine test material can be visually recognized.

Further, the predetermined test agents selected from the above-mentioned reagent groups are test agents whose measurement is carried out by changing the hue, so that the selected test agents have different hue changes. For this reason, as shown in FIG. 1, the urine test test materials having different shapes and sizes are impregnated with the test agents A to F having different hue changes as described above, respectively. From the shape and size and the change in hue, the urine test items and the results can be easily confirmed visually.

The mixture of the urine test material thus obtained, that is, a health check material, is sprayed on a predetermined place such as an animal urinating area such as a pet toilet. If an animal such as a pet urinates on the urine, the urine is applied to each urine test material. This makes it possible to simultaneously perform a urine test for each of the test agents contained in the health check material. Since the test agent impregnated in the above various urine test materials is measured by the change in hue as described above, the health of animals such as pets can be easily checked by observation. This health check method is effective for checking the health of non-human animals that cannot collect urine arbitrarily.

[0032]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The test material for urinalysis according to the present invention will be specifically described below.

[Measurement of Water Absorption] As a highly water-absorbing material, cellulose sponge (made by Toray Industries, Inc., cubic (5 mm × 5 m
m × 5 mm)), porous cellulose particles (manufactured by Rengo: trade name Biscopearl, shape: spherical, particle size 4 mm),
And pulp balls (pulp ball-type cat sand [trade name: flower field: manufactured by Pet Line Co., Ltd.]) were measured for water absorption by the following method. Table 1 shows the results.

On the other hand, for comparison, the water absorption of cellulose acetate particles (manufactured by Nakarai) and N-pulp (manufactured by Rengo) were measured in the same manner. Table 1 shows the results.

Measurement of water absorption About 10 g of a carrier for measuring water absorption is taken and immersed in 500 ml of water. After 5 minutes, the carrier is taken out and weighed.

[0036]

[Table 1]

[Examples 1 to 3] Hue change test As a glucose test agent, a combination of glucose oxidase and peroxidase with 4-aminoantipyrine and naphthol disulfonic acid was used as described below.

EDTA-2Na 2mg 4-aminoantipyrine 5mg 1-naphthol-3,6-disulfonic acid Na 16mg Peroxidase 6mg Glucose oxidase 25mg Dihydrogen phosphate 8mg Hydrogen phosphate 2Na 4mg Water 10g The above components were mixed, Each 10 g of the above-mentioned cellulose sponge, porous cellulose particles or the above-mentioned pulp ball was immersed. Then, it dried at 50 degreeC and manufactured the test material for glucose test.

20 g / g of the obtained test material for glucose measurement
The glucose solution of mg / dl was sprayed and left. The change in hue after 1 minute, 5 hours, and 17 hours was observed. Table 2 shows the results.

[Comparative Examples 1 and 2] Instead of the porous cellulose bead particles, the above-mentioned N pulp or cellulose acetate-based particles were used, and the glucose solution was immersed in a glucose test agent in the same manner as in Example 1. I sprayed. Table 2 shows the results.
Shown in

Results From Examples 1 to 3, the change in hue caused by glucose was maintained after 17 hours, whereas in Comparative Examples 1 and 2, the hue of purple disappeared after 5 hours, and Had become. This is presumably because the water absorption of Comparative Examples 1 and 2 was small, so that the retained glucose was not sufficiently retained, and the impregnation amount of glucose was reduced after 5 hours.

[0042]

[Table 2]

[Example 4] When a plurality of urine test materials were used Among the glucose test agents used in Example 1, a glucose test was conducted by replacing the 1-naphthol-3,6-disulfonic acid Na with phenol. This was used as a medicine, and was impregnated with 10 g of the porous cellulose particles. In addition, 10 g of the above cellulose sponge was impregnated with a BTB solution.

1 g of the obtained porous cellulose particles and 1 g of cellulose sponge were mixed, and a 20 mg / dl glucose solution adjusted to pH 8 was sprayed on the mixture and allowed to stand. As a result, the hue of the spherical particles changed vividly from white to red, and the hue of the angular particles changed from yellow to blue through green.

RESULTS A glucose test comprising a combination of 4-aminoantipyrine and phenol changes color from white to red in the presence of glucose. In addition, the BTB solution shows yellow at pH 6 or lower, but turns green at pH 7 and blue at pH 7.6 or higher.

Therefore, the above-mentioned mixed urine test agent test material clearly shows the change in hue of each test agent, and by combining with the shape of the urine test agent test material, the type of the test agent and the hue of the test agent are obtained. Was clearly recognized.

[0047]

According to the present invention, since a material having a high water absorption is used, a large amount of urine and a urine test agent can be held. For this reason, a change in hue due to a predetermined component of urine of the urine test agent clearly appears, and urinalysis becomes easier.

When a hydrophilic material is used as the highly absorbent material, the urine water absorption rate is increased. Thereby, the urine test agent comes into contact with the urine test agent instantaneously, and the hue changes quickly.

[Brief description of the drawings]

FIG. 1 is a schematic diagram of a health check material according to the present invention.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI G01N 33/68 G01N 33/68 33/72 33/72 A 33/84 33/84 A (72) Inventor Masaaki Kajino Osaka, Osaka 3-12-22 Kitahama, Chuo-ku, Ribason Co., Ltd. (72) Inventor Nobuyuki Kawachi 3-1-21, Kitahama, Chuo-ku, Osaka-shi, Osaka Ribason Co., Ltd. (72) Mao Fujita Sakai-gun, Fukui Prefecture 1-8-10 Jiyugaoka, Kanazu-cho Rengo Co., Ltd. Fukui Research Laboratory (72) Inventor Akio Tanaka 1-8-10 Jiyugaoka, Kanazu-cho, Sakai-gun, Fukui Prefecture Fukui Research Laboratory Rengo Co., Ltd. (72) Inventor Hiromi Fujita Fukui Prefecture 1-8-10 Jiyugaoka, Kanazu-cho, Sakai-gun Rengo Co., Ltd. Fukui Research Laboratory

Claims (5)

[Claims] 1. The amount of water absorption per 10 g is 15 g to 500 g.
A test material for urinalysis comprising a highly water-absorbent material g, and a urine test agent. 2. The urine test material according to claim 1, wherein the superabsorbent material is porous cellulose particles. 3. A urine test material according to claim 1 or 2 having a different shape or a different size, wherein the urine test material has a specific shape and size. For each urinalysis test material that you have, a pH test, a glucose test, a protein test, an occult blood test, a ketone test,
A health check material impregnated with one different test agent selected from specific gravity test agents and urea test agents. 4. The above-mentioned pH test agent, glucose test agent, protein test agent, occult blood test agent, ketone body test agent, specific gravity test agent,
4. The health check material according to claim 3, wherein the predetermined test agent selected from the urea test agents is a test agent whose measurement is performed based on a change in hue, and the selected test agents have different hue changes from one another. 5. 5. A method for checking the health of an animal, comprising spraying the health check material according to claim 3 or 4 at a predetermined location and simultaneously performing a urine test for the test agent contained in the health check material.