WO2004008139A1

WO2004008139A1 - Absorbent for diagnosis and absorption article

Info

Publication number: WO2004008139A1
Application number: PCT/JP2003/008814
Authority: WO
Inventors: Hiroaki Maeda; Koji Kawaguchi; Hiroshi Itayama
Original assignee: Sanyo Chemical Industries, Ltd.
Priority date: 2002-07-12
Filing date: 2003-07-10
Publication date: 2004-01-22

Abstract

An absorbent for diagnosis which contains a water absorption resin (A) and a diagnostic agent such as a pH diagnostic agent, a protein diagnostic agent, a glucose diagnostic agent or an occult blood diagnostic agent, wherein the above resin (A) contains one or more monomers selected from the group consisting of aliphatic unsaturated monocarboxylic acid amides, alkylene oxides and alkylated products and alkyl etherified products of the same, optionally together with one or more monomers selected from the group consisting of unsaturated anionic monomers, unsaturated cationic monomers and salts thereof as constituting monomer(s) and has an acid or base equivalence of 2 meq/g or less determined by neutralization titration at pH 4 to 9; and an absorption sheet for diagnosis of animals or humans which is made up of the above-described absorbent, a fiber, a nonwoven fabric, a paper, a synthetic resin film, etc.

Description

Description Diagnostic absorbers and absorbent articles Technical field

The present invention relates to an absorbent material for diagnostic use for animals or humans, and more particularly, to an absorbent material, an absorbent sheet and the like which can be used for medical examinations, disease diagnoses and pregnancy tests between animals or humans. The present invention relates to a diagnostic absorbent article used. Background art

Conventionally, a test material for urinalysis comprising a water-absorbent resin having a water absorption of 10 to 550 g per 10 g containing a urine test agent (Japanese Patent Laid-Open No. 11-295306). And a urine test diaper characterized by impregnating the inner surface of a diaper with a reaction reagent that changes color according to the components in the urine (Japanese Patent Application Laid-Open No. 200-289845) has been proposed. .

An object of the present invention is to provide a diagnostic absorbent that is excellent in persistence of color formation.

Another object of the present invention is to provide a diagnostic absorbent having a high coloring speed.

A further object of the present invention is to provide a diagnostic absorbent excellent in chronological stability (stability of diagnostic reagent).

Still another object of the present invention is to provide a diagnostic absorbent capable of diagnosing cancer.

Still another object of the present invention is to provide an absorbent sheet for diagnosis and an absorbent article having excellent persistence of coloring. Still another object of the present invention is to provide a diagnostic absorber and an absorbent article having a high coloring speed. Disclosure of the invention

The above and other objects of the present invention are the first embodiment of the present invention, which is a water-absorbent resin (A) (hereinafter also referred to as (A)) and a diagnostic agent (M) (hereinafter also referred to as (M)) And (A) having at least 60 times the water-absorbing ability.

Another object of the present invention described above and below is a second embodiment of the present invention, comprising a water-absorbent resin (A) and a diagnostic agent (M), wherein (A) is an aliphatic unsaturated monocarboxylic acid. One or more monomers (al) selected from the group consisting of amides, alkylene oxyside adducts of aliphatically unsaturated monocarboxylic acids, and their alkylated or alkyl etherified compounds, and optionally More than one kind of monomer (a 2) selected from the group consisting of more unsaturated anionic monomers, unsaturated cationic monomers and their salts, is contained as a constituent monomer. This is achieved by a diagnostic absorbent characterized by having an acid or base equivalent of not more than 2 milliequivalents Zg determined by neutralization titration of H4-9.

Another object of the present invention described above and below is a third embodiment of the present invention, wherein at least 40% by weight of the water-absorbent resin (A) and 25 ppm by weight based on the weight of (A). 2. 5% of diagnostic agent (M), (M) supported on a support or coated with a water-soluble or water-disintegrable coating material, (A) supported or coated (M) Diagnostic absorbers, which are obtained by mixing

Another object of the present invention described above and below is a fourth embodiment of the present invention, wherein a water-absorbent resin (A) and a diagnostic agent for antigen-antibody reaction (M 0) (hereinafter referred to as (M 0)) ), Which can be achieved by a diagnostic absorbent.

Another object of the present invention described above and below is a fifth aspect of the present invention, a diagnostic absorbent sheet having the following (1) or (2) and a BZF separation layer (C):

(1) a layer (B 1) carrying a water-absorbent resin layer (AL) and a labeled antibody (M y) or a labeled antigen (M X); or

(2) A layer (B 2) in which a labeled antibody (My) or a labeled antigen (M x) is supported on a water-absorbent resin (A). BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a diagram schematically showing a cross section of urine U and a part of a diagnostic absorbent sheet of the present invention. AL is a water absorbing resin layer, B 1 is a layer carrying a labeled antibody, and C 1 is a B / F separation layer.

FIG. 2 is a diagram schematically showing a cross section of a part of an absorbent sheet in which urine U has penetrated into the AL layer and the B1 layer.

FIG. 3 is a diagram schematically showing a cross section of a part of the absorbent sheet in which urine U has penetrated to reach the B / F separation layer C 1.

4 to 6 are diagrams schematically showing a part of the cross section of the absorbent sheet of the present invention. 〇F is a front film, C 2 is a BZF separation layer, D is a color former layer, and U F is a back film. P in Fig. 6 is a hole.

FIG. 7 is a cross-sectional view of a part of the absorbent sheet prepared in Examples 5 and 6. BEST MODE FOR CARRYING OUT THE INVENTION

Water absorbent resin (A)

The water-absorbent resin (A) that can be used in the present invention has a weight of 10 to 2,0. It is a hydrophilic cross-linked polymer that can absorb 100 times water.

These polymers are usually hydrophilic groups such as carboxylic acid (salt) groups (ie, carboxyl and / or carboxylate groups), sulfonate (salt) groups, and phosphoric acid (salt) groups. It has a tertiary amino group, a quaternary ammonium base, a hydroxyl group or a polyethylene oxide group. Preferable (A) includes the following.

(1) Starch-acrylic acid described in JP-B-53-46199

(Salt) Darafto cross-linked copolymer.

(2) Crosslinked polyacrylic acid (salt) obtained by aqueous solution polymerization (adiabatic polymerization, thin film polymerization, spray polymerization, etc.) described in JP-A-55-133334.

(3) For the reverse phase suspension polymerization described in JP-B-54-30710, JP-A-56-26909 or JP-A-11-15808. The resulting crosslinked polyacrylic acid (salt).

(4) Copolymers of vinyl esters and unsaturated carboxylic acids or their derivatives as described in JP-A-52-14689 or JP-A-52-27455 Saponified.

(5) The copolymerization of acrylic acid (salt) and sulfonic acid (salt) group-containing monomer described in JP-A-58-23212 or JP-A-61-36309. Polymer.

(6) The crosslinked isobutylene-maleic anhydride copolymer described in US Pat. No. 4,389,513.

(7) A hydrolyzate of a starch-acrylonitrile copolymer described in JP-A-46-43995.

(8) A crosslinked carboxymethyl cellulose derivative described in US Pat. No. 4,650,716.

(9) Water-swellable polyurethane, and the urethane is arranged on ordinary rubber. The combined one.

Of these (A), the preferred is at least 60 times its own weight, more preferably 60 to: I, 000 times, especially 80 to 1, 000 times, In particular, a water-absorbent resin (A 1) (hereinafter also referred to as (A 1)) capable of absorbing water 100 to 1,000 times as much as water, and an aliphatic unsaturated monocarboxylic acid Or an alkylene oxide adduct (all) (hereinafter, also referred to as (al1)) of the above, or an alkylated or alkyl ether amide (al2) (hereinafter, (al2) )) And one or more monomers (a1) (hereinafter also referred to as (a1)) selected from the group consisting of: an unsaturated carboxylic acid, an unsaturated sulfonic acid, One or more monomers (a 2) (hereinafter, also referred to as (a 2)) selected from the group consisting of saturated phosphoric acids and their salts are defined as constituent monomers. Water-absorbent resin having an acid or base equivalent of less than 2 milliequivalents / g, preferably less than 1 milliequivalent, (A 2) (hereinafter also referred to as (A 2)).

As (A1), a starch-acrylic acid (salt) graft crosslinked copolymer and a crosslinked polyacrylic acid (salt) are preferred. (A1) has the advantage that the color development duration after the color development of the diagnostic agent is long.

Water absorption can be measured by the following method:

(A 1) is added to a tea bag of known weight (specified in JIS, K7223) with a size of 20 cm in length and 10 cm in width made of nylon mesh of 250 mesh. Add 2 g and immerse in 500 mL of deionized water. After 5 minutes, remove the tea bag and weigh it to determine the weight of water absorbed. Calculate the water absorption capacity from the following formula.

Water absorption capacity (times) = [weight of absorbed water (g)] no 0.2 (g) (1) For example, if it can absorb 60 times its own weight of water, its water absorption capacity is 60 times.

(A1) preferably has an absorption rate of less than 80 seconds, especially less than 70 seconds. It is preferred that the absorption rate be less than 80 seconds, since the time from the start of absorption of the body fluid to the development of the diagnostic agent is short.

The absorption rate can be measured by the following measuring method:

Pour 5 OmL of physiological saline into a 100 ml beaker, adjust the temperature to 25 ° C, and stir at 600 rpm with a magnetic stirrer. Into this, (A 1) 2.0 g was quickly injected into the vortex, and at the same time, measurement with a stopwatch was started. The time (seconds) required for the vortex to disappear and the liquid level to become horizontal is defined as the absorption rate.

(A 1) is preferably 8 to 20 g Z g or more, especially 10 to 18 g Z g, 40 g Z cm ² absorption under load for sheet and paper use applications Quantity (hereinafter abbreviated as Ab-L). If A b — L is 8 to 20 g Z g, problems such as liquid return rarely occur. A b —L can be measured by the method described in US Pat. No. 6,156,678.

Of (al) constituting (A 2), (all) is an amide of an aliphatic unsaturated monocarboxylic acid (having 3 to 5 carbon atoms, hereinafter abbreviated as C). For example, (meta) acrylamide, crotonic acid amide] and alkylene oxide (C2-4) adduct (addition mole number 1 to 200) [(meta) acrylyl Acid adducts of 1 to 100 moles of ethylene oxide, maleic acid adducts of 2 to 50 moles of ethylene oxide, etc.].

As (a12), the alkyl (Cl-4) diamide of (a11) [mono or dimethyl (meth) acrylyl amide, monoisopropyl (meta) ata linoleamide Etc.), and (a11) alkyl (C1-4) ether compound [ethylenoxide of (meth) atalylic acid with 1 to 100 moles Methyl etherified product of the additive, etc.].

Among (a 2), examples of the unsaturated anionic monomer include unsaturated carboxylic acids, unsaturated sulfonic acids, unsaturated phosphoric acids and salts thereof, and the following are exemplified. .

The unsaturated carboxylic acids include the above-mentioned aliphatic unsaturated monocarboxylic acids, C 9 to 12 aromatic monocarboxylic acids [such as cinnamic acid and vinyl benzoate] and C 4 to 12 unsaturated dicarboxylic acids. [Maleic acid, fumaric acid, itaconic acid, aconitic acid, etc.].

Unsaturated sulfonic acids include alkene (C 2-6) sulfonic acid [such as vinyl sulfonic acid and (meth) aryl sulfonic acid], unsaturated aromatic (C 6-18) sulfonic acid (styrene sulfonic acid and α —Anolecenyl and alkyl (C 1-18) alkenyl esters of methyl styrene sulphonic acid, etc., and sulfocarboxylic acids (such as monosulfoalkanoic acid and snolephoconodic acid) [methyl vinyl, propyl (meta) aryl and Stearyl (meta) arylsulfosuccinate and (meta) arylsulfophosphate, etc., sulfo (hydroxy) alkyl (C2-6) (meta) atalylate and equivalent ( (Meta) Acrylylamide [Sulfoethyl and sulfopropyl (meta) atalylate, + 3 — (meta Acryloyloxy 1- 2-hydroxyprononsulfonic acid, 2- (meta) acrynoreamid 2-, methylprononsulfonic acid and 3-(meta) acrylyl amide 1-hydroxypropanesulfonic acid Etc.].

Unsaturated phosphoric acids include (meta) atalyloyloxyalkyl (C2-24) mono- and diphosphates, [2— (meta) atalyloyloxyxethyl phosphate and Hue-Lu 2 — (meta) Atari Loiro Kishechyl phosphate etc.].

These salts include alkali metals (sodium, potassium and lithium). ), Alkaline earth metal (such as calcium and magnesium) salts, ammonium salts, organic amines (such as dimethylamine, trimethylamine, jetanolamine, triethanolamine), and quaternary salts Ammonia (such as tetramethylammonium) and the like.

Among (a 2), unsaturated cationic monomers and their salts include:

C 3-6 alkenylamine (arinoreamin, crotylamine, etc.), unsubstituted or mono- or di-alkyl (Cl-6) aminoalkyl (C2-6) (meta ) Attalylate or (meta) acrylate amide [aminoethyl (meta) atalylate or (meta) acrylyl amide, dimethylaminoethyl (meta) acrylate or Are (meth) acrylyl amide, acetylaminoethyl (meta) acrylate, or (meth) acrylamide, morpholineethyl (meth) acrylate, and the like. Acids (such as hydrochloric acid and sulfuric acid) salts and organic acids (force of C 1 to 12, rubonic acid: acetic acid and propionic acid, etc.)

(A 2) has an acid or base equivalent of less than 2 milliequivalents, preferably less than 1 milliequivalent Zg, as determined by neutralization titration at pH 4-9. By being less than 2 milliequivalents / g, the diagnostic accuracy of the diagnostic agent (M), particularly the pH diagnostic agent (Ml) and the protein diagnostic agent (M2) described below is improved.

The acid or base equivalent of pH 4-9 can be measured as follows.

A 0.5 g sample of the water-absorbing resin is precisely weighed, and 100 mL of ion-exchanged water is added, followed by stirring for 30 minutes. Thereafter, 3% (hereinafter, unless otherwise specified,% represents% by weight) of an aqueous hydrogen chloride solution is added dropwise, and the pH is adjusted to 3 to 4. Titrate with an NZ10NaOH aqueous solution using a potentiometric titrator, read the titration ml number from pH4 to pH9, and calculate by the following formula (2). Acid or base equivalent 2 (fXAmL) / (10XS) (2) (where f is the factor of N / l ON a OH, AmL is the titration mL, S is the weight of the water-absorbent resin (g) Represents.)

Shiino preferred among (A 2) is 9 0-1 0 0 mole ^_0/0, especially 1 0 0 mole ⁰ / o units of (a 1), 0 to: 1 0 mole ⁰ /. , Especially 0 mol ⁰ /. (A2) and 0.01 to 3 mol% of the unit of the crosslinkable monomer (b). (A 1) and when used in combination with (a 2), (a 1 ) 9 0 to 9 9 mol ^_0/0, the (a 2):! Rere Shi preferred is to 1 0 mol ^_0/0.

The crosslinkable monomer (b) is a compound having 2 to 4 or more functional groups capable of reacting with (a1) or (a2), and is a bis (meth) acrylamide or poly (meth) acrylamide. All di or polyunsaturated carboxylic esters, and polyol di or poly (meth) aryl ethers, and US Pat. No. 5,728,792 The compounds described are exemplified.

The water absorption capacity of (A 2) (measuring method is the same as that of (A 1)) is preferably 10 to 1000 times, more preferably 15 to 800 times.

The shape of (A) may be granular (spherical, granular, crushed, etc.), needle-like, flake-like, block-like, agglomerated form in which granular primary particles are fused together, and form-like. In particular, they are granular, needle-like, flaky and agglomerated.

When (A) is granular or aggregated, the preferred size is that when the absorbent described below is used as cat litter, a particle size of 90% or more of (A) is preferable. :! ~ 850im, especially 3 ~ 500μπι. This range is preferable because the absorption rate is particularly high.

When the absorbable sheet described below is used as a urine sheet for dogs, 90% or more of (Α) is preferably 38 to 118 μμπι. In particular, it is 63-10000. If it is more than 38 μπι, gel blockage is less likely to be generated in the sheet when it comes into contact with an aqueous solution, and the absorption rate is improved. If it is not more than 118 μm, the surface area per volume of the absorbing material does not become small, and the absorbing speed tends to be high.

The size of the granular or agglomerated (A) was measured using a rotary test sieve shaker and a sieve specified in JISZ8801-2000. The method can be performed according to the method described in the “Chemical” Engineers ”Handbook, 6th Edition (Mac Glow Hinole Book, Kannony, pp. 1984-21, page 21). Is based on this method.

The average particle size (weight average particle size, hereinafter abbreviated as Dav) is used as (A) for absorbent articles for humans (diapers, urine collection pads, etc.). A mixture of two types of particles, each of which uses a tap sieve, the same applies hereinafter), is preferable because it can achieve both an absorption rate and prevention of moisture return after water absorption.

The water-absorbent resin having a smaller Dav (Α ′) (hereinafter also referred to as (Α ′)) preferably has a Dav of 50 to 400 m, particularly 100 to 350 m, It mainly contributes to the absorption rate. If it is 50 m or more, gel blocking is unlikely to occur, and if it is 40 μm or less, the surface area increases and the absorption rate tends to improve. The water-absorbent resin with a large Dav (Α '') (also referred to as (Α '')) is preferably 410-750 μηι, especially 450-650 μηη Dav Having. If it is 410 μm or more, the return of water after water absorption tends to be small, and if it is 75 μm or less, it is easy to exhibit sufficient water absorption capacity.

The weight ratio of (A ′) :( Α ′ ′) is usually (3: 7) to (7: 3), preferably (4: 6) to (6: 4).

When (Α) is used as an absorbent in combination with the occult blood diagnostic agent (Μ4) (hereinafter also referred to as (Μ4)) described below, the stability of peroxides contained in (Μ4) is stabilized. Water-soluble inorganic salt based on the weight of (ii) is preferably 2% or less, more preferably 1% or less, and especially 0.3% or less, for the purpose of maintaining its properties over time. It is. Examples of the inorganic salts include catalyst residues [sulfate (sodium sulfate, calcium sulfate) and sulfite (sodium sulfite, calcium sulfite)], and other inorganic salts (salts). , Calcium chloride, magnesium chloride, etc.).

To reduce the inorganic salts, for example, add (A) to a large amount of ethanol water = 1/1 (weight ratio), stir well, filter through a nylon mesh (eg, 250 mesh), This can be achieved by adding a large amount of the same solvent to the filtration residue and repeating the same operation several times.

The inorganic salt content can be measured as follows.

Add 2.O g of (A) to 1 L of ethanol / water = 11 (weight ratio) at 25 ° C, stir gently for 30 minutes, and then use a nylon mesh of 250 mesh. Filter, add 1 L of the same solvent to the filtration residue, and repeat the same operation 4 times. About 5 L of the obtained solvent is concentrated to 500 mL with an evaporator, and anion chromatography and cation groups are quantified with an ion chromatograph, and the total is defined as the inorganic salt content. Diagnostic (M)

(M) includes a qualitative or quantitative amount of the component to be detected in a body fluid (urine or blood), or a diagnostic agent that diagnoses by measuring a characteristic value of urine.

In the present invention, the "diagnostic agent" includes a part or all of the reagents involved in the diagnosis, that is, a compound that reacts with a component in a body fluid, and, if necessary, a labeling substance or a coloring agent. included.

(M) includes the following.

pH diagnostic agent (Ml): For example, methyl red Z bromthymol blue (BTB) (in the following, / represents a combination).

Protein diagnostics (M 2): For example, tetrabromphenol blue (TB PB) / Nouchenic acid dihydrogen potassium (for measuring albumin content). Glucose diagnostics (M 3) (hereinafter also referred to as (M 3)): For example, glucose oxidase peroxidase Z color former (carboxylate, o-tridin, 3-amino-6-c) Roll 9-dimethylaminopropylcarbazol hydrochloride, 2,7-diaminofluorin dihydrochloride or N- (3-sulfopropyl) -1,3,3 ', 5,5'-tetramethylbenzidine Li).

Occult blood diagnostic agents (M4): For example, tamenyl hydroperoxydono o-triidine, citrate buffer 2,5-dimethylhexane 1,2,5—dihydrobaroxydotetramethylbenzidine, tamene hydroperoxide / Tetra methylbenzidine hydrochloride.

Pyrirubin diagnostics (M5): for example, 2,4-dichloroaniline, 2,6-dichlorobenzeneazoniummute trafluoroborate, or 2—trifluorobenzenebenzenediazoumute Borate.

Perobilinogen diagnostic agent (M 6): For example, p-dimethylaminobenzaldehyde, 4-methoxybenzenediazodidimethyl tetrafluoroborate, 3,4-methylenedioxybenzene 1-1 Mono-diazo-tetrafluoroborate or 4-fluoro-3-nitrobenzene difluorotrifluoromethylsulfonate.

Nitrite diagnostics (M7): eg N- (1-naphthyl) ethylenediamin 'dihydrochloride, 3-hydroxy-1,2,3,4-tetrahydro-7,8-benzoquinoline, or N— (1—Naphthylamino) One 3—Prono. Sunsolefonic acid.

Leukocyte diagnostic agent (M8): For example, indoxycarboxylic acid ester.

Specific gravity diagnostic agent (M 9): For example, BTB / methoxyethylene maleic anhydride copolymer or BTB polyacrylic acid. Amylase diagnostics (M 10): For example, carboxymethyl starch remazolamide.

Ascorbic Acid Diagnostics (Mil): For example, Methylene Green Dutranolered, 2,6—Dichlorophene-Norenolein Defeno-Norhenium. Salt diagnostics (Ml 2): For example, silver chloride 2,7_dichlorofluorescein.

Ketone body diagnostic agent (M13): For example, a two-mouthed pursidona triduniuni hydrate.

(M 0) is a reagent for diagnosing a disease using an antigen-antibody reaction, and is an antibody (y) immobilized on a support (hereinafter also referred to as (y)) or an antigen (X) (Hereinafter referred to as (X)) binds to (X) or (y) in the body fluid to form a conjugate, and the conjugate is further labeled with an antibody (M y) (hereinafter referred to as (M y)) or a labeled antigen (M x) (hereinafter also referred to as (M x)), and diagnosed based on a change in the color of the label, or as necessary, a coloring agent (d) Thus, a diagnostic agent utilizing a so-called two-site sandwich measurement method based on the principle that the label can be colored to make a diagnosis based on the degree of color development can be given.

(X) and (y) include the antigens and antibodies described in US Pat. No. 5,073,485.

Among (X), preferred are protein-related substances, particularly CEA, TPA, polyamine, β2-microglobulin, Ι-Ι, γ-GTP, FDP, from the viewpoint of simplicity of measurement. , Amylase, ct1 antitrypsin, esterase, hemoglobin, amylase, albumin, globulin and NAG; and hormone-related substances, especially HCG, E3 and FDP.

Among (y), preferred are IgA and microglobulin. Different types of labels can be used depending on the diagnostic method. No. 5,073,485. Preferred are chemiluminescent substances, and especially enzymes, latexes and metals.

For example, when the labeling substance is a chemiluminescent substance (eg, acrylonitrile ester, N-aminobutyl-N-ethylethylisonorenol (ABEI), etc.) And a method of reacting a solution of the prepared antigen with a sulfonate (Japanese Patent Application Laid-Open No. H05-340946).

For example, when the labeling substance is an enzyme (for example, penoleoxidase, anorecalyl phosphatase, gnorecosoxidase, β-galactosidase, etc.), And Ν—succinimide 1— (Ν—mice lime) After reacting cyclohexane 1 11 ノレレレ画画、画分分分分分分分にて分マ画マAnd then fractionating it with a gel filtration column (Biochemical Experiment 27 Enzyme Labeling (Eiji Ishikawa: Gakkai Shuppan Center)). When the labeling substance is latex (for example, SBR latex), 0.5 mg of the antibody is added to a buffer (pH 7.0 to 9.0), dissolved, and 10% latex particles (usually Add 0.5 mL of 0.3-0.5 μηι), allow the reaction to proceed (for example, at 25 ° C for 1 hour), and then centrifuge (for example, 5 ° C, 100,000 rpm, (30 minutes), and put the sediment in a stabilizing agent (Block Ace (Dainippon Pharmaceutical Co., Ltd.)) to prepare 0.2% latex-labeled antibody.

If the labeling substance is a metal (for example, a method using gold colloid), it is reported by Lot & Geeuze (Eur. J. Cell Biol., 38, 87, 18985). There is a method that has been.

The binding amount and the binding ratio of the labeling substance are usually 70 to 100% based on the number of moles of the labeled antibody. When the label is an enzyme, (d) is required for coloring the label, and (d) is a redox coloring agent [ortho-trizine, 1,2-phenylenediamine Fluorescent reagents [4—hydroxyphenylacetic acid, 3— (4—hydroxyphenyl) propionic acid, etc.], bioluminescent reagents [2—nitrophenyl'β—Ό-galactoside, noreciferi And its derivatives], and chemiluminescent reagents [luminol and its derivatives (such as phenylaminobutyl-ethylethyl sorbinol)] and the like. The amount of (d) is usually 100 to 100,000,000 times based on the equivalent weight of the label.

Diagnosis according to (d) is performed by spraying, applying, dipping, or dropping a coloring aid to develop color, and observing or measuring the coloring intensity with a visual inspection device (spectroscopic analyzer, etc.).

In addition to (d), reagents related to the color reaction such as oxidizing agents (peroxides, etc.) and enzymes (luciferase, glucose 16-phosphate dehydrogenase, etc.) are used simultaneously as color-forming aids. May be.

The weight ratio of the color-forming aid to (d) is preferably from 1 Z 1 to 100. Diagnostic absorber

The diagnostic absorbent of the present invention is composed of (A), (M) and, if necessary, other constituent materials.

The shape of the absorbent material is granular (spherical, granular, crushed, etc.), needle-like, flake-like, block-like, or agglomerated, in which primary particles are fused to each other. Needles, needles, flakes and aggregates are preferred.

As for the size of the absorbent, the major axis and the minor axis are usually from 5 to 20 m, and preferably from 15 to: L O, Ο Ο Ο μ ιη.

The absorbent of the present invention comprises (Α) and (Μ) supported on it. Absorbents; and absorbents (M) and (A) supported on a support other than (A) or coated with a water-soluble or water-degradable coating material.

In the present invention, “support” means that (M) is also present on the surface of the support and, if necessary, inside the support, and “coating” means that (M) is present only inside the support. This is what you are doing.

Of (M), the components to be supported or coated may be all of the components of (M), or some of the components of (M).

For example, in the case of (M 0), a method of spraying or applying (M y) and (d) at the time of diagnosis / judgment by forming an absorbent made of (y) carried thereon, a method of spraying or applying (d) at the time of diagnosis / judgment, and carrying (y), coated (y) A method of forming an absorbent material consisting of My) and (d).

Absorbent materials in which (M) or a part of (M) is covered with a coating material are superior in that the storage stability can be improved compared to an absorbent material in which all of (M) is supported on a support. This is particularly preferred when (M) contains enzymes or peroxides.

Examples of the support other than (A) include the following fibers, granules, and chips.

The fibers include natural fibers, man-made fibers and synthetic fibers, mixtures thereof, and regenerated fibers thereof, and the following are exemplified.

Natural fiber [cotton, absorbent cotton, fibrous rattle, straw, grass charcoal, wool, microfibril, nocteria cellulose, hardwood bleached kraft pulp (LBKP), softwood bleached kraft pulp (NBKP), and other flapping pulp (E.g., waste materials from the manufacture of disposable diapers), artificial fibers [rayon and acetate, etc.], synthetic fibers [polyolefin fibers (Polyolefin fibers) Polyethylene fiber, polypropylene fiber, polyethylene / propylene composite fiber, etc.), polyester fiber (polyethylene terephthalate fiber and polyethylene terephthalate.polyethylene ethylene phthalate copolymer composite fiber, etc.), Polyolefin / polyester composite fibers (polyethylene'polyethylene terephthalate composite fiber and polypropylene'polyethylene terephthalate composite fiber, etc.), polyamide fibers (polyethylene terephthalate ethylene diamide fiber, polyisoterephthalic acid Ethylene diamide fiber, polyterephthalic acid propylene diamide fiber, polyisoterephthalic acid propylene diamide fiber, etc.), polyacrylic fiber (polybutyl acrylate fiber and polyacrylic acid 2-ethylhexyl)維等), and poly Ata Li Roni preparative drill fibers.

Of these, natural fibers and synthetic fibers are preferred, and natural fibers are more preferred, particularly cotton, absorbent cotton, and fibrous rags, from the viewpoint that the strength of the absorbent is easily provided. Is waste from the production of pulp or disposable diapers.

Further, the particulates include inorganic particulates and organic particulates, and inorganic particulates are preferable.

The composition of the inorganic particulate matter includes oxides (such as silicon oxide, aluminum oxide, iron oxide, titanium oxide, magnesium oxide, and zirconium oxide), calcium carbonate, kaolin, tanolek, mai power, and bentonite. Glass, powder, balloon, glass (powder and balloon), glass, graphite, metal (iron, copper, aluminum and gold, etc.), zeolite and aspect.

Two or more of these may be used in combination, or two or more of these may be combined. Of these, preferred are silicon oxide, anodic aluminum oxide and titanium oxide, and more preferred are silicon oxide and oxidized oxide. Noremium, especially amorphous silicon oxide.

Inorganic particulates include porous inorganic particles (P 1) and non-porous inorganic single particles (P 2).

Examples of the porous inorganic particles (P 1) include hollow, porous, petal, aggregated, and granulated particles.

The preferred Dav of the porous inorganic particles (P 1) is from 0.1 to: Ι Ο Ο Ομπι. The preferred Dav of the non-porous inorganic single particles (Ρ2) is 0.03 to 0.07 / m, and more preferably 0.01 to 0.054 μπι.

Examples of the organic particulates include particulates made of natural, semi-synthetic or synthetic resins other than (ii).

Natural resins include mannan, starch, plant mucilage (arabia gum, guar gum, locust bean gum, carrageenan, etc.), seaweed-derived products (sodium alginate, agar (galatatan), etc.), and microbial mucilage. (Dextran, levan, etc.), protein (gelatin, casein, collagen, keratin, etc.), granular oataz, and the like.

Semi-synthetic resins include cellulosic derivatives such as viscose, methylcellulose, ethinoresenololose, hydroxysenorelose, canoleboximetinolenose, canolepo'ximetinolethynoresenorelose, nitrosenorelose, and cationizer. Senollose, acetinolate cellulose, senorelose succinate, etc., starch derivatives (acetylated starch, acrylated starch, solubilized starch, carboxymethyl starch, dialyzed starch, oxidized starch, cationized starch, dextrin, etc. ), Pulp (hardwood bleached kraft pulp (L Β Κ Ρ), softwood bleached kraft pulp (Ν Β Κ Ρ), flap pulp, etc.).

Examples of the synthetic resin include thermoplastic resins (polyamide resin, polyester resin, polyurethane resin, polyolefin resin, polystyrene resin, and acrylic resin). Examples thereof include granular resin (including powder) of a thermosetting resin (epoxy resin, urea resin, phenol resin, and the like) or a resin such as a rill resin, a polyvinyl alcohol, or a polyether resin.

Examples of chips include pulp chips and chip-like sawdust.

The mode of loading of (M) may include physisorption, chemisorption or chemical bonding.

The method for supporting (M) on a support includes the following method. (1): Powder or liquid (M) is simply mixed with the support. (2): The support is impregnated with water of (M) or a mixed solvent solution of water and a hydrophilic organic solvent, and then dried.

In each step, if necessary, heating (preferably at 30 to 90 ° C) can promote adsorption or binding.

Absorbents obtained by physical adsorption by the above method (2) include, for example, (M) such as bromthymol blue, and water and a hydrophilic organic solvent (eg, methanol, ethanol, or acetate). Dissolve in a mixed solvent of pH or pH buffer to make a solution, add (A) to it, and gently stir at room temperature for a certain period of time (usually 1 to 120 minutes). After sufficient swelling, it can be obtained by drying under reduced pressure (40-60 ° C). The preferred concentration of (M) based on the weight of the solution in the solution is usually 10 ppm to 50%, preferably 50 ppm π! ~ 30%.

When a support other than (A) is used, the weight ratio of the support Z (A) is preferably 95/5 to 55/45, more preferably 80/20 to 6 0/40.

When all the components of (M) or a part of (M) are coated with a coating material, the coating material may be a water-soluble or water-disintegrable polymer, One or more amphiphilic substances which form micelles, vesicles or liposomes in aqueous solution can be used.

Examples of the water-soluble or water-disintegrable polymer include the aforementioned seaweed-derived substances, proteins (gelatin, casein, etc.), and cellulosic derivatives (methylcellulose, ethynoresenorelose, hydroxy). Such as sonololose and canoleboximetinolecellulose), solubilized starch, polyvinyl alcohol, and polyoxyalkylene oxide.

Examples of the amphiphilic substance include known surfactants (for example, those described in US Pat. No. 4,331,447), as well as amino acids, phospholipids, and the like. Biosurfactants such as bile salts, cyclodextrin derivatives, crown ether derivatives and the like.

As a coating method, a method of spraying or applying a coating material from the outside of (M) to harden it, and a method of coating (M) with a vesicle of the coating material in a solvent (water and Z or the above-mentioned hydrophilic organic solvent) are used. , Trapping in micelles or ribosomes and then removing the solvent.

The absorbent of the third embodiment is composed of (A) and (M) supported and / or coated, and includes a mixture of both, and a molded product thereof. The method of mixing may be powder mixing, or liquid mixing in a solvent followed by solvent removal.

The absorbent of the present invention may further contain other constituent materials. Other constituent materials include, for example, the aforementioned fibers, granules, chips, and the like.

Other additives include preservatives, fungicides, antibacterial agents, antioxidants, ultraviolet absorbers, coloring agents, fragrances, deodorants, and U.S. Patent No. 5,743,213. One or more selected from the additives described in this document can be added. The content of each component based on the weight of the absorbent of the present invention is as follows.

(A) is preferably at least 40%, more preferably at least 60%, especially at least 70%.

When (A) is at least 40%, it is preferable in that a sufficient diagnostic speed and sufficient water return preventing property after water absorption can be maintained.

Also, (M) (however, only those that are supported or coated) are preferably 1 pp π! ~ 4%, especially 10 ppm force, etc. 1%.

The content of the support other than (A) is 0:! To 60%, preferably 3 to 50%.

Other constituent materials are 0 to 60%, preferably 5 to 50%.

Other additives are 0.0000 to 1: 10%, especially 0.0000 to 5%.

5% is preferred.

The content of (M) based on the weight of (A) is preferably 2.5 ppm to 10%, and more preferably 25 ppm. ~ 2.5%.

As a method for obtaining the absorbent of the present invention,

(1): (A) carrying (M) (or a mixture of (A) with (M) another supporting or coated support) and, if necessary, other constituent materials and / or A method of granulating other additives (hereinafter abbreviated as a substrate including all of them) by adding a small amount of water while mechanically stirring, and

(2): A method of molding a substrate by pressure molding.

Examples of devices that can be used in (1) include a Nauta mixer, a Ribon mixer, a conical blender, a mortar mixer, and a universal mixer. Examples of the method of adding water include a method of spraying water on a substrate, a method of blowing steam, and a method of absorbing moisture while maintaining the substrate at high humidity. Monohydric alcohol in water as needed (E.g., methanol, ethanol, isopropyl alcohol), polyvalent ethanol (ethylene glycol, propylene glycol, glycerin, etc.) polyalkylene (2-4 carbon atoms) glycol (poly) Ethylene glycol, etc.), polyvinyl alcohol, the above-mentioned surfactants, etc. can be added for the purpose of improving the effect as a binder and the effect of water permeating into the base material and granulated material. it can.

The amount of water added depends on the type of substrate, but is usually 1 to 30%, preferably 2 to 15%, based on the weight of the substrate. When the amount of water to be added is 30% or less, the granulated material is not too soft, and the shape of the granulated material is less likely to be broken and the granulated materials are less likely to stick to each other. On the other hand, if the amount of water to be added is 1% or more, the granulation speed is high and a granulated product having a uniform shape is easily formed.

When the diagnostic agent is (M 3), it is preferable to keep the water content at 3% or less, particularly 2% or less, for the purpose of stabilizing the enzyme activity for a long period of time. In order to suppress the water content to 3% or less, a method of drying the absorbent under reduced pressure, adding a desiccant such as silica gel, and storing the absorbent in a bag may be used.

Examples of the method (2) include a method in which the base material is pressed into a pellet shape or the like in a mold having an appropriate shape and size, or a sheet shape, a rod shape, or a simple shape. Can be formed by pressure-molding into a block shape, and then cutting or pulverizing to the desired size and shape. The pressure molding may be performed under a force S normally performed at normal temperature, a calo temperature (for example, 30 to 300 ° C.), or humidification (for example, 50 to 100% R.H.). The pressure at the time of pressure molding is a force S which can be appropriately selected according to the type, particle size or properties of the base material, usually 1 to 3, 000 kg / cm ² , preferably 10 to 2, 0 0 0 K g Z cm ² . Rolling press machines (compacting press machine, pre-cutting press machine, etc.), piston press machine, and screw press machine —Pressure molding machine or perforated plate extrusion molding machine can be used.

Among the shapes of the absorbent obtained by molding as described above, preferred are a sphere, a pellet, and a column.

Among the absorbent materials of the present invention, the absorbent material of the first embodiment is for animal diagnosis, and the absorbent material of the other embodiments is for animal and human diagnosis.

The absorbent of the present invention can be suitably used as it is as an absorbent for animal diagnosis, for example, an absorbent for pets, particularly cat sand. Diagnostic absorbent sheet

The diagnostic absorbent sheet for animals or humans composed of the absorbent material according to the first to fourth aspects of the present invention comprises the above absorbent material, and further comprises fibers, nonwoven fabric, paper and synthetic resin film. It is an absorbent sheet consisting of one or more members selected from the group.

These absorbent sheets include single-layer sheets consisting of one sheet containing the absorbent material and multilayer sheets consisting of two to five or more layers.

As the fibers, the above-mentioned fibers can be used.

Non-woven fabrics include non-woven fabrics made of thermoplastic resin (for example, polyolefin resin, polyester resin or polyolefin-polyester copolymer), and paper made of cellulose fiber (Japanese paper and As the film, a film made of the above thermoplastic resin is preferably used.

The size of the absorbent sheet is usually an area of ² 5~ 2 5 0 0 cm 2 of rectangles, squares, and the like circular or oval, thickness 0. 5~ 5 0 mm, good or lay Ha :! ~ 20 mm.

For example, the absorbent sheet is obtained by simultaneously mixing the above-mentioned absorbent material and fiber in an air stream. Manufacture by combining and stacking fibers, mixing absorbent and fiber in advance and stacking in airflow, stacking absorbent on fiber, and stacking fiber can do.

An apparatus used in these methods includes, for example, a drum forming apparatus.

The basis weight of (A) contained in the absorbent sheet {content of (A) per unit area of the absorbent sheet} is 10 ~ :! OOO g Zm ² is preferred, and more preferably 50 to 700 g Zm ² .

When the basis weight is in this range, the water retention capacity of the absorbent sheet is further increased, and there is a tendency that even more excellent dryness and moisture resistance under load are exhibited.

The basis weight of the fibers contained in the absorbent sheet (excluding the fibers contained in the absorbent material) is 10 ~ :! OOO g Zm ² is preferred, particularly preferably 50 to 70

It is 0 gm ² .

The content of the absorbent in the absorbent sheet is preferably 40 to 99.9%, more preferably 62 to 80%, based on the weight of the absorbent sheet. . Within this range, the absorbent sheet can be made thinner. The preferred structure of the absorbent sheet is a liquid-permeable front film (hereinafter abbreviated as OF) and a liquid-impermeable back film (hereinafter, referred to as “OF”) on both sides of the absorbent sheet.

U F).

For example, a structure in which OF is arranged in the uppermost layer, UF is arranged in the lowermost layer, and an absorbent sheet (one layer) composed of the above-mentioned absorbent and fiber is arranged as an intermediate layer (K)

1), OF is arranged in the uppermost layer, UF is arranged in the lowermost layer, and (K

2) to 4 layers of the same absorbent sheet as in the case of 1), and provide one or more liquid-permeable intermediate films (hereinafter abbreviated as CF) between each absorbent sheet as necessary. In the structures (K 2) and (K 1) or (K 2), at least between the uppermost layer and the intermediate layer and / or between the intermediate layer and the lowermost layer. Another example is a structure in which one type of CF is arranged (K 3) (in this case, the diffusivity of the bodily fluid absorbed by the absorbent sheet is easily improved).

Of these, (K 3) is preferable from the viewpoint of leakage of the absorbent from the absorbent sheet.

The OF is not particularly limited as long as bodily fluids that come into contact with the absorbent sheet easily penetrate and reach the internal absorbent material. For example, paper made of cellulose fiber (such as Japanese paper and tissue paper) ), Non-woven fabric, woven fabric or knitted fabric made of thermoplastic resin, etc., and perforated film made of thermoplastic resin (for example, polyolefin resin, polyester resin or polyolefin'polyester copolymer). It is preferably used. The hole diameter of the apertured film is preferably 0.01 to 1 mm.

The thickness of the 〇F is preferably 5 to 500 // m, particularly preferably 20 to 750 111.

The preferred range of the material, pore diameter and thickness of CF is the same as that of OF.

The UF is not particularly limited as long as it can prevent the bodily fluid absorbed by the absorbent sheet from seeping out to the back surface.For example, a film made of the aforementioned thermoplastic resin or the like is used. Let's do it. Of these, sheets made of polyolefin are preferred, more preferably sheets made of polyethylene, polypropylene or polyethylene / propylene, particularly preferably made of polyethylene. It is a sheet that can be obtained. The thickness of U F is preferably in the same range as that of O F described above.

The absorbent sheet of the present invention can be suitably used, for example, as a urine sheet for dogs. The diagnostic absorbent sheet according to the fifth embodiment of the present invention comprises the following (1) Is an absorbent sheet for diagnosis having (2) and a BZF separation layer (C) (hereinafter also referred to as (C)).

(1) Water-absorbent resin layer (AL) (hereinafter also referred to as (AL)) and layer (B 1) (hereinafter referred to as (B 1)) having (My) or (M x) supported on a support. U); or

(2) A layer (B 2) carrying (My) or (M x) on (A) (hereinafter also referred to as (B 2)).

In the above (1), (AL), (B1) and (C) are all layered, and these layers are laminated to form the absorbent sheet of the present invention.

In the above (1), (AL) is a mixture of (A) and the above-mentioned fiber, nonwoven fabric, paper, or the like, or a layered composite. In (B 1) in (1) above, the labeled antibody-carrying layer (B My) (hereinafter also referred to as (BMy)) in which (My) is supported on the support and (M x) are supported on the support Labeled antigen-bearing layer (BM x) (hereinafter also referred to as (BM x)). Among (B1), the preferred one is (BMy).

Examples of the material of the support constituting (B1) include the aforementioned fibers, paper (eg, filter paper), nonwoven fabric, synthetic resin, and the aforementioned natural resin. The shape of the support may be any of sheet, powder, granule and fiber. Preference is given to sheets, powders and granules.

In (2) above, (B 2) and (C) are both layered, and these layers are stacked to form the absorbent sheet of the present invention.

(B 2) is obtained by using (A) as a support and carrying (My) or (M x) on (A).

(B) [Hereinafter, (B) including (B1) and (B2) is referred to as (B). The dimensions are usually less than 30 x 30 cm, preferably less than IOXIOC m, especially from 3 x 3 cm to 1 x 1 cm, such as rectangles, squares and circles. Although the preferred size and shape are different depending on the type and size of the human being or the animal, the viewpoint is that (M y) or (M x) must be completely released by body fluids. However, the area is usually smaller than (AL) (for example, an area of 1 to 5 to 100), and is an area through which a single body fluid of the object sufficiently penetrates. The thickness is usually between 0.1 and 10 mm, preferably between 0.1 and 3 mm.

The method for supporting (M y) or (M x) on a support includes water, a buffer solution (eg, pH 6.8, 0.02 M phosphate buffer), a hydrophilic organic solvent, and the like. (For example, alcohol-based solvents such as methanol and ethanol, ketone-based solvents such as acetone and methylethyl ketone, etc.) and water, or a mixed solvent of a buffer and a hydrophilic organic solvent, (My ) Or (M x) is dissolved (100 ppb to 20% by weight), mixed with the support or impregnated in the support, and dried under reduced pressure or freeze-dried.

Incidentally, (M y) or (M x) supported on the support is generally released from the support at the same time as the body fluid permeates.

The amount of to be supported per unit area of the layered body of the support (M y) or (M x) is favored properly is _{0 1 11 § (:. 111} 2 ~ 1 0 0 111 8 0 11 2, and the Ku ^{1 O ng Z cm 2 ~ l} 0 mg / cm Demel.

The ratio of (M y) or (M x) based on the weight of (A) is preferably 0. lppb ~: 1,000 ppm, and more preferably 100 ppm from lppb force. ppm.

(B) is preferably in the range of 0.01 to 3 mL / Z, particularly preferably in the range of 0.05 to 2.

It has a permeation rate of 5 mLZ (25 ° (: 1 atm). Within the range, the diagnosis speed is high and the diagnosis can be performed with high accuracy.

The water permeability can be measured by the following method.

(1) In a room adjusted to a humidity of 65% at 25 ° C, (B) was sandwiched by a glass type> (AD VAN TEC, KG S-47), which is a filter holder for vacuum filtration. Weigh the set (F 0) precisely (up to 1 mg unit), secure the set with clamps so that the filtration surface is horizontal, and install a receiver (do not perform depressurization). )).

(2) Next, about 300 g (W O) of ion-exchanged water of 25 ± 1 ° C weighed precisely (up to 1 mg unit) is injected from above and the stopwatch is started.

(3) At the elapse of 60 minutes, the water adhering to the lower branch of the filter is quickly wiped off to remove, and the weight of the final holder set with unfiltered ion-exchanged water remaining (FW) Is measured.

(4) The permeability rate is calculated by the following formula (3).

Permeability rate (m LZ min) = water filtration (g) ÷ water specific gravity _{(g / / m L) ÷} 6 0 ( min)

(3) However, the amount of filtered water (g) = {F0 (g) + W0 (g)}-FW (g), and 25 ± 1. As the specific gravity of C water, a value of 1.0000 (g / mL) is used.

(C) is a conjugate formed by an antigen-antibody reaction between (M y) or (M x) and (X) or (y) in a body fluid [hereinafter, referred to as a labeled conjugate. (M xy)] and unreacted (M y) or (M x).

(C) has a BZF separation layer (C 1) (hereinafter referred to as “BZF separation layer”) having an immobilized antigen (f X) or an immobilized antibody (fy) that reacts with unreacted (M y) or (M x). (Also referred to as (C 1)), and a BZF separation layer (C 2) (hereinafter also referred to as (C 2)) that separates (M xy) depending on the pore size of the separation layer. Preferred is (CI). (CI) usually contains (X) or (y) of the same type as (X) or (y) in the body fluid to be diagnosed, either on the surface or inside the support. Has been immobilized. As the shape and material of the support, those similar to the support described in the above (B1) or (B2) are exemplified. Preferred examples thereof include sheet-like materials such as paper (eg, filter paper), and the like. It is a sheet made of granular water-absorbent resin and pulp.

The amount of (X) or (y) immobilized on (C 1) is preferably 0.1 g / cm ² to 500 mg / c per unit surface area of the layered material of the support. lng / cm ² to 250 mg Z cm ^2, which is larger than the amount of (M x) or (My) carried on (B) [for example, the amount carried on (B) 1.2 to 10 times, preferably 1.5 to 5 times).

As a method for immobilizing (X) or (y) on the support, there are a method of physically adsorbing and a method of bonding by a chemical reaction. Examples of physical adsorption include the method of physically adsorbing (X) to a support having high adsorptivity to protein, such as a cellulose ester (sheets such as cellulose acetate), and a chemical reaction. For this purpose, a support (eg, a sheet of nitrocellulose) is silylated with γ-aminophenol. N-sulfosuccinimidyl-41 (Methylmethyl) cyclohexane-111-carboxylate, and a cross-linking reaction with an amino group present in (X).

The (C 2) is a sheet or film, having a molecular weight cut off smaller than the molecular weight of S (MX y), and is an unreacted (X) or (y) molecule. Those larger than the amount are preferred. The molecular weight cut off of (C 2) is preferably 100,000 to 300,000, especially 30,000 to 200,000, and (C 2) Difference between the molecular weight of the fraction and the molecular weight of (M xy) It is preferable that the absolute value of the difference between the molecular weight of the fraction and the molecular weight of the unreacted (X) or (y) is 5,000 or more.

As (C2), commercially available products (for example, Diaf 1 o UM series (Am icon), Penoleto LaFino letter Q series (Ad Vantech Toyo), etc.) are also used. it can.

The water permeation rate in (C) is related to the antigen-antibody reaction rate in the absorbent sheet. For example, if (C) is placed at the bottom of the absorbent sheet and the sample liquid is dropped on the top, the lower the water permeation rate of (C), the lower the (X) or (y) force S (fy) or The number of times that (fx) can be contacted increases, the reaction rate increases, and the resolution in (C) increases. However, if the water permeation speed becomes extremely slow, the leaching speed of the separated liquid will decrease, and the time required for measurement will increase.

(C) preferably has a water permeation rate (25 ° C, 1 atm) of 0.01 to 3 mLZ, especially 0.05 to 2.5 mLZ. Same as (B) above). Within this range, BF separation is easily performed and the diagnostic speed is high.

The absorber of the present invention may further comprise a color former layer (D) (hereinafter also referred to as (D)), OF and Z or UF.

Examples of (d) carried on (D) include those described above, and examples of the support include those having the same shape and material as those exemplified in (B1) or (C) above. Is exemplified. The same goes for the preferred ones.

Loading amount of (d) is per unit surface area of the support, favored properly is ^{1 0 0 fmo 1 / cm 2} ~ 5 0 0 mm o 1 / cm ", and Ku in 1 pmo 1 / cm ~ 5 0 mmo 1 / cm.

Examples of the method of supporting the same include the same method as the method of supporting (M y) or (M X) on the substrate as exemplified in (C 1) above.

Diagnosis by (D) is color development after reaching (My) or (X) (D) The reaction is performed, and the color intensity is observed or measured with a visual inspection device (such as a spectroscopic analyzer). The color-forming reaction is carried out by carrying all reagents involved in the color-forming reaction on (D) and developing without post-treatment (dl), (D) to (M y) or (X) after reaching (X). Either method (d 2), in which a part of the relevant reagents are added later to develop color, may be used.

In (D) in the case of (d1), in addition to (d), the above-described color-forming auxiliary may be supported simultaneously. The amount of the color-forming aid is preferably the same weight as the amount of (d) carried, and 1/100.

In the case of (d 2), (D) may be taken out and a coloring reaction may be carried out by spraying, applying, dipping or dripping a coloring aid. Observation or measurement of the coloring intensity may be performed with (D) after the coloring reaction fixed at the original position, or may be taken out and performed.

The same OF and UF as described above can be used as the OF and UF used as needed.

The order of the essential constituent materials (B) and (C) in the absorbent sheet according to the fifth embodiment of the present invention is usually such that the side to which the body fluid is supplied (hereinafter referred to as the body fluid side) ) Is arranged, and (C) is arranged outside. Also, the position of (AL) is preferably on the fluid side of (B), between (B) and (C), outside of (C), and in combination of two or more of these positions. Yeah.

The absorbent sheet according to the fifth embodiment of the present invention is obtained by laminating each layer of (AL), (B), (C) and (D) if necessary, in the above order, and then obtaining the desired size. It can be manufactured by cutting, or by producing layers of the desired size and shape for each layer, and then laminating or bonding each layer.

Examples of diagnosis using an absorbent sheet according to the fifth embodiment of the present invention are shown in FIGS. This will be described in Section 3.

The absorbent sheet shown in FIG. 1 is composed of a B / F separation layer (C) having a water-absorbent resin layer (AL), a layer (B1) supporting a labeled antibody (My), and an immobilized antigen (fx). 1).

When urine (U) containing the antigen (X) comes into contact with the absorbent sheet of FIG. 1, (U) penetrates as shown in FIG. (U) that has penetrated is partially absorbed by (AL), but part of it penetrates into (B1) to release (My), and (X) and (My) in urine are lost. Reacts to produce (M xy).

The (U) partially containing unreacted free (M y) penetrates further and reaches (C 1) as shown in FIG.

Here, the free (My) is reacted with (fx) and immobilized to (C1). On the other hand, (M x y) passes through (C 1) because it no longer reacts with (f x).

Therefore, the concentration of (M xy) exiting through (C 1) is correlated with the concentration of (X) in (U). Therefore, the diagnosis can be made by measuring the concentration of (Mxy) by the colorant method or the like.

The absorbent sheet shown in FIG. 4 is composed of the layer of FIG. 1 described above, and further comprises a color former layer (D), a front film (OF) and a back film (UF).

The absorptive sheet shown in FIG. 5 includes a layer (B 2) carrying the labeled antibody (My) on the water-absorbent resin (A), a layer (C I) having the immobilized antigen (fx),

F) and (U F).

The absorbent sheet shown in FIG. 6 has a configuration using (C 2) which separates according to the size of the pore diameter instead of (C 1) in the configuration of FIG. The diagnostic absorbent sheet of the present invention can be used as it is as an excrement disposal material. Further, a pellet-shaped excrement disposal material composed of a water-absorbent resin and a fibrous material on an absorbent sheet (for example, one described in Japanese Patent Application Laid-Open No. Hei 7-325756) or sand, etc. A known excrement disposal material made of a material (such as a plate-like material with a hole or a mesh-like material) may be laminated or sprayed. Diagnostic absorbent articles

The disposable diaper, the pet toilet, the simple toilet, the portable toilet, the sanitary napkin, the incontinence pad and the wound sheet according to the present invention may be any one of the above-mentioned absorbent or absorbent sheets, trays, It is composed of a bag, a nose or a bottle, and is a diagnostic absorbent article.

The pet toilet for the present invention is (1) a cat sand placed on a tray or the like, (2) a urine sheet placed on a tray vinyl sheet, A combination of (1) and (2) (a urine sheet is laid on the tray, a net-shaped tray is placed on top of it, and cat sand is placed on top of it. Composite type toilet).

Paper mummets include those in which an absorbent body is provided with gathers or the like to enhance the adhesion to the abdomen, waist, thighs, or crotch, and a mounting tape or the like to prevent slipping. The material and type of the gather and the mounting tape are not particularly limited, and known materials used for conventional paper mats can be used.

Diagnosis using an absorbent sheet or absorbent article can be performed by checking the color change by opening the film with a structure that can open and close (OF) or a part of (UF) as necessary. Diagnose.

INDUSTRIAL APPLICABILITY The absorbent sheet and absorbent article of the present invention can be used for terrestrial animals and animals or humans, and are bred, for example, for pets and livestock. It can be suitably used for animals, especially mammals and birds.

Among the mammals, pets include dogs, cats, egrets, monkeys, nomsters, fillets, raccoons and risks, and livestock such as horses, horses, hibiscus, and pigs. And other bred mammals include wild boar, fox, deer, camel, zodia and kirin.

Among the birds, pets include canaries, parakeets, notes, wild birds and birds, and other bred birds include birds, ducks, fish, hawks and crows. can give.

Humans include children (including newborns, babies and infants) and adults (including the elderly). Example

Hereinafter, the present invention will be further described with reference to Examples, but the present invention is not limited thereto.

Example 1 Absorbent for measuring specific gravity of urine (Absorbent of 1 'embodiment)

Preparation of absorbent material:

Dissolve 5 g of BTBO and 2.5 g of polyacrylic acid (Mw = 100, 500) in 500 mL of 0.02 M phosphate buffer (pH 7). A diagnostic solution was prepared.

Water-absorbent resin "Aquapearl AP211DS" (Acrylic acid-based polymer, manufactured by Sun Diamond Polymer Co., Ltd .: Water absorption capacity = 600 times, absorption speed = 40 seconds, Ab-L = 1 (0 g / g, Dav = 210 μm, granulated) Add 100 g of the above solution to 100 g, stir well with a glass rod, and incubate at 50 ° C at 0.05 MPa. It was dried under reduced pressure for 24 hours. After drying, the partially blocked portion was crushed with a glass rod to obtain an absorbent material. (The crushing of the blocking portion is also performed in the following Examples and Comparative Examples. T). When the Dav of the absorbent was measured, it was 2 13 μm, and it was granular, making a standard color sample:

An appropriate amount of salt was added to urine of a dog with a specific gravity of 1.005 measured by a weighing-type hydrometer, and the specific gravity was 1.010, 1.020, 1.025, and 1.030. A standard urine was prepared.

A standard color sample was prepared by adding 25 g of each standard urine to 5 g of the above-mentioned absorbent (absorbent one hour after preparation). The sample with a specific gravity of 1.005 was green, and the yellow color increased as the specific gravity increased. The color samples were stored in a glass bottle with a volume of 70 mL with a lid (hereinafter all color samples were stored in the same container). Measurement of urine specific gravity:

The specific gravity of each sample obtained by adding 5 g of the above-mentioned absorbent to 25 g of urine of eight dogs was compared with a color sample, and the specific gravity was visually determined.

For example, if the color of the sample is between the color samples of specific gravity 1.015 and specific gravity 1.020, the color tone was observed and the specific gravity was determined in increments of 0.001. . The judgment was made by three people and the average value was taken as the specific gravity.

The color development speed was visually confirmed from the time urine was added to the time color development was completed, and the time required was measured.

After the color development was completed, the absorbent was allowed to stand in an open state, and the time required for the specific gravity reading to change by 0.010 was measured to evaluate the color development continuity.

On the other hand, the specific gravity of the above eight large urines was measured with a floating-type hydrometer.

The correlation coefficient between the specific gravity measured with the absorbent of the present invention and the specific gravity measured with the floating-type hydrometer was 0.87 (n = 8), and a good correlation was obtained. It was demonstrated that the specific gravity of urine can be measured with the light absorbing material. Table 1 shows the results.

Table 11

Comparative Example 1;

Preparation of absorbent material:

Instead of “Aquapearl AP2 1 1 DS”, “Sunfresh AT — 30” (acrylamide-based polymer, manufactured by Sanyo Chemical Industries, Ltd .: water absorption capacity = 45 times, absorption rate = 100 seconds, Ab-L = 7 g g Dav = 1200 μm, crushed, in the same manner as in Example 1 except that an absorbent (Dav 1210 Aim) was used. , Crushed). In addition, the absorbent is used A color sample was prepared in the same manner as in Example 1 except for the fact that the absorbent was used, and the specific gravity of dog urine was measured in the same manner as in Example except that the absorbent was used.

However, as compared with Example 1, it was found that the coloring speed was slow, the coloring was unstable, and the persistence was poor.

The correlation coefficient between the specific gravity measured with the absorbent of the comparative example and the specific gravity measured with the floating specific gravity meter was 0.63 (n = 8), indicating a low correlation, and high accuracy depending on the absorbent of the comparative example. It turned out that urine specific gravity could not be measured. The results are shown in Tables 1-2.

Table 1 — 2

Example 2 Absorbent for measuring glucose (absorbent according to the first embodiment) Preparation of absorbent material:

In 5 mL of 0.02 M phosphate buffer (pH 6), add 10 mg of glucose oxidase (from Aspergillus sp., 100 u / mg) and 10 mg of phenol oxidase (from Horseradish, 4 5 0 u / mg)

Dissolve 10 mg to make enzyme solution.

To "Aqua Pearl AP 2 1 1 DS" 1 0 0 g, o - the door Li Jin 5 weight ^_0/0 methanol solution 1 0 g was added the total amount, after which the mixture was stirred well with a glass rod, 2 5 Dede 0 It was dried under reduced pressure at 0.5 MPa for 1 hour. Thereafter, drying was performed at 105 ° C using an infrared moisture meter, and it was confirmed that the drying loss rate was 0.07%. In addition, if there is a partially blocked area, pulverize it with a glass rod, spray 5 mL of the above enzyme solution with stirring, and reduce the pressure to 0.05 MPa at 25 ° C. Was dried under reduced pressure for 24 hours to obtain absorbent material-1 (Dav = 2 12 μm: granular). The drying loss rate of the absorbent was measured with an infrared moisture meter, and it was confirmed that the moisture content was 1.20%.

Further, water was added to this absorbent material to make the water content 6.5%, thereby producing an absorbent material 2 (Dav = 2 15 μm: granular). Creating a standard color sample:

Urine test paper “Pr e t e s t” (W ako P ur eCh e m i c a

). Dissolve D-dalcos in urine of glucose-negative (1) cats at 1 1 nd., Ltd.), 100, 250, 500, 100, and 200, A standard urine of mg / dL was prepared. The standard urine was glucose (soil) urine, glucose (+) urine, glucose (2+) urine, glucose (3+) urine, and glucose (4+) urine. . 25 g of each standard urine (use 5 hours after preparation of standard urine, unless otherwise specified, the same applies to the following standard urine) To 5 g of the above-mentioned absorbent for measuring glucose (1) or 2 (above 1 hour after the preparation of the absorbent, unless otherwise specified, also apply to the following absorbents) A color sample was created. Blueness increased as the concentration of glucose increased.

Measurement of glucose content:

For a sample in which 5 g of the above-mentioned absorbent for glucose measurement 1 or 2 was added to 25 g of each of the urine of 8 cats, the budo-sugar concentration was compared with the color sample. ) Visual judgment was made in six stages of (4) to (4+). Judgment was performed by three people, and the results of each judgment were averaged (for example, (+) in the case of (+), (10), or (2+), judged as (+)), and the bud sugar concentration of each sample was determined. And

The color development speed was visually checked from the time urine was added to the time color development was completed, and the required time was measured. .

After the color development was completed, the absorbent was allowed to stand in an open state, and the time required for the sugar to change by one or more steps from the judgment value of the sugar after the color development was measured to evaluate the color development continuity.

On the other hand, in the urine of the above eight cats, the concentration of glucose was measured by “Pretest” and a six-step judgment was performed.

The glucose concentration measured with the absorbent of the present invention and the glucose concentration measured with the urine test paper were in agreement with eight samples, demonstrating that the glucose concentration could be measured with the absorbent of the present invention. The results are shown in Table 2-1 and Table 2-2. Two

Using urine test paper Measurement with the absorbent material of Example 1-1 (moisture 1.2%) Measurement result of urine No. Measurement result Coloring speed Coloring persistence Average value (seconds) (minutes)

2 +

9 2 + 2 + 2 + 7 2 9 3

2 +

3 +

1 0 3 + 3 + 3 + 7 9 8 7

2 +

Chief

1 1 8 8 8 3 Sat

+

1 2 + + + 7 5 8 6

+

Earth

1 3 person Sat 8 4 7 9 person

+

1 4 + 2 + + 8 7 8 3

+

4 +

1 5 4 + 4 + 4 + 8 3 8 6

3 +

2 +

1 6 2 + 2 + 2 + 8 1 9 1

2 +

Average value (all match) 80.4 86.0

Table 2-2

Also, after preparing Absorbent-1 and Absorbent-2 above, the glucose concentration was adjusted in the same manner as above except that the absorbent stored at 5 ° C or 50 ° C for 3 months was used. It was measured. The results are shown in Tables 2_3 and 2_4.

twenty three

Measurement with urine test paper using absorbent material of Example 2-1 (water 1.2%) Measurement result of urine N o.

Measurement result Measurement result

Average value Average value

2 +

9 2 + + 2 + 2 +

2 + +

1 0 3 + 3 + 3 + 3 +

2 + 3 +

±

1 1 Sat Sat

Earthworker

+ Chief

1 2 + + + +

+ +

Sat Sat

1 3 Shi Sat Shi

Sat Sat

+ Soil

1 4 + + + + +

+ +

4 + 3 +

1 5 4 + 4 + 4 + 4 + 4 +

3 + 4 +

2 + 2 +

1 6 2 + 2 + 2 + 2 + 2 +

2 + +

(Match all) (match all)

Table 2 _ 4

It has been found that the absorbent of the present invention has a small change over time even after 3 months at 5 ° C storage and can be measured sufficiently. I understand. Comparative Example 2;

Creation of absorbent material:

Absorbent (1.1% moisture: Dav = 12) in the same manner as in Example 2 except that “Sunfresh AT-130” was used instead of “Aquapearl AP211 DS”. 0 m) and a color sample, and Similarly, the glucose concentration in cat urine was measured.

However, there was a large variation in the coloring speed, the coloring was unstable, and the color sometimes faded over time (after several minutes).

The glucose concentration measured with the absorbent of the comparative example and the glucose concentration measured with the urine test strip only match 6 out of 8 samples, and the accurate absorption of glucose cannot be measured with the absorbent of the comparative example. I understood.

The results are shown in Table 2-5.

Table 2-5

The glucose concentration was measured using the absorbent stored at 5 ° C or 50 ° C for 3 months in the same manner as in Example 2 except that the absorbent of the comparative example was used. Was measured. The results are shown in Table 2-6,

Table 2 _ 6

Example 3 Absorbent for Protein Measurement (Absorbent of Second Embodiment) Preparation of Absorbent:

SANFRESH AT-03, a water-absorbent resin (polymer mainly composed of acrylamide, (a1) content: 90-99 mol ⁰ /., Sanyo Chemical Co., Ltd.) ) Is crushed with a mixer (Mitsubishi Electric, JM-K31) for 10 minutes, passed through a 242 μιη wire mesh, and the particulate matter remaining on the 109 m wire mesh is collected. 0 3 Fine-grained product (water absorption capacity 40 times, Dav = 1 85 m, An acid equivalent of 0.9 milliequivalent / g) was obtained by neutralization titration of PH 4 to 9. A diagnostic solution of 11.7 g of formic acid, 11.5 g of potassium dihydrogen citrate, and 9.5 g of TBPB in 50 g of water was mixed with a Dav = 360 μm silica solution. Add 10 g of powder to 100 g of Sunfresh AT-0.3 fine-grained product, mix well with a glass rod, and reduce the pressure at 50 ° C at 0.05 MPa for 24 hours. After drying, the absorbent was obtained (Dav of the water-absorbing resin portion was 24.5 μm, and Dav of the silica pad portion was 36.3 μm). Creating a standard color sample:

The sialicbumin was dissolved in protein-negative (1) cysteine urine using "Pretest" to prepare standard urine of 15, 30, 100, and lOOOOmgZdL. The standard urines were protein (sat) urine, protein (+) urine, protein (2+) urine, and protein (3+) urine. A color sample was prepared by adding 25 g of each standard urine to 5 g of the above absorbent material. When protein was present, the color changed from yellow to green, and the greenness increased as the protein concentration increased. Protein measurement:

A sample in which 5 g of the above absorbent material was added to 25 g of each of the eight urine samples was visually judged by comparing the protein concentration with the above five stages in comparison with a color sample.

The determination was made in the same manner as in Example 2.

On the other hand, the protein concentrations of the above eight samples of urine urine were measured using “Pretest” and a five-step judgment was performed.

The protein concentration measured with the absorbent of the present invention and the protein concentration measured with the urine test paper were the same in eight samples, and the protein concentration was measured with the absorbent of the present invention. It was proved that it could be set. Table 3 shows the results. Comparative Example 3;

Preparation of absorbent material:

Except that "Sunfresh AT-0.3" was replaced by "Sunfresh AT-0.3" (acid equivalent 9.7 milliequivalents / g by neutralization titration at pH 4 to 9). An absorbent material and a color sample were prepared in the same manner as in Example 3, and the protein concentration of the urine was measured in the same manner as in Example 3.

However, the protein concentration measured with the absorbent material of the comparative example and the protein concentration measured with the urine test paper matched only 5 out of the 8 samples, and the urine which was negative on the urine test paper became a false positive. In some cases, false positive urine was determined to be positive. It was found that the absorbent of the comparative example could not measure protein concentration with high reliability.

Table 3 shows the results.

Table 3

Example 4 Absorbent for Occult Blood Measurement (Absorbent of Third Embodiment) Preparation of Absorbent:

Dissolve 1 g of peroxide in mouth with 25 g of methanol, add 5 g of silica powder having a Dav of 3600 μπι to Q, and use a vacuum drier at 25 ° C and 0.0 After drying at 5 MPa for 1 hour, a silica powder supporting cumene peroxide was prepared. Thereafter, 50 g of water was added, stirred and sufficiently dispersed, and 15 g of water-soluble starch (“Pine Flow S” manufactured by Matsutani Chemical Co., Ltd.) was added. In addition, the operation of adding 5 g of methanol and stirring for 1 minute was repeated 10 times. Then, a total of 50 g of methanol was added. As the amount of methanol added increased, the starch dissolved in the aqueous solution became insolubilized and adsorbed on one surface of the silica powder. The dispersion was filtered through filter paper (Toyo Filter Paper, No. 2), and the solid content was recovered. The solid was dried at 50 ° C. under a reduced pressure drier at a temperature of 0.05 MPa for 1 day. Coated granules (Dav = 945 m, granules) were prepared.

“Aquapearl AP211DS” 45 g and water-absorbent resin “Aquapearl DS — 50 T” (manufactured by Sundia Polymer Co., Ltd .: water absorption capacity = 600 times, absorption speed = 48 seconds, Ab — L = l 2 g Z g, Dav = 350 μm) 55 g (total of 100 g as water-absorbent resin) and 10 g of o_trigin 5% methanol solution Add and stir well with a glass rod, 25 °. The mixture was dried under reduced pressure at 0.05 MPa for 1 hour. The above-mentioned peroxide-coated powder is added to the mixture, and the mixture is mixed with an absorbent (Dav = 2 13 ^ m granular water absorbent resin, Dav = 354 ix m granular water absorbent resin and Dav). = 945 μm of a mixture with a granular peroxide coating). Creating a standard color sample:

The human hemoglobin obtained by removing the plasma part from normal human blood was dissolved in human urine with negative occult blood (一) by “Pretest” to obtain 0.06, 0.15, 0.75. A standard urine of mg Z d L was prepared. The standard urine was occult blood (+) urine, occult blood (2+) urine, and occult blood (3+) urine. A color sample was prepared by adding 25 g of each standard urine to 5 g of the above-mentioned absorbent (absorbent one hour after preparation). As the occult blood concentration increased, the bluish color increased. Occult blood measurement:

For the sample obtained by adding 5 g of the above absorbent material to 25 g of each of the eight human urine samples, the occult blood concentration was divided into the above four stages as compared with the standard color sample. It was visually determined. The determination was made in the same manner as in Example 2.

On the other hand, the occult blood concentration of each of the above eight human urine samples was measured using “Pretest”, and four-step judgment was performed.

The occult blood concentration measured with the absorbent of the present invention and the occult blood concentration measured with the urine test paper were in agreement with eight samples, demonstrating that the occult blood concentration can be measured with the absorbent of the present invention. The results are shown in Table 4-1.

The color development speed was visually checked from the time urine was added to the time color development was completed, and the required time was measured.

The dry feeling was evaluated by the following method. Place absorbent paper (5 cm x 5 cm) on a polyethylene film (5 cm x 5 cm), spread the absorbent after urine addition on it to a height of 2 mm, and place it on it. Polyethylene finolem (5 cm x 5 cm) and polypropylene non-woven fabric (5 cm x 5 cm) were placed in this order (total thickness of 2.5 mm). The dry feeling was evaluated from the top of the non-woven fabric by touch feeling evaluation. Three people evaluated the following five-point scale to determine the average value.

Rating 1; The liquid floats with just a light touch

Rating 2: The liquid feels wet when touched lightly, and floats when strongly touched. Rating 3; Touch slightly to feel slightly damp, touch strongly to feel wet. Rating 4: There is no damp feeling when touched lightly, but damp feeling when strongly touched. Rating 5: No feeling of damp even if strongly touched, good feeling of try. Table 41

Comparative Example 4;

Creation of absorbent material:

Using 100 g of “Aquapearl AP211DS” in the same manner as in Example 4, a dried product of a water-absorbent resin containing o—trizine was obtained. g was dissolved in 25 g of methanol, and the mixture was stirred well with a glass rod. Then, the mixture was dried under reduced pressure at 25 ° C. for 1 hour at −0.05 MPa, and an absorbent for occult blood measurement (Dav = 2 14 m, granular).

A color sample was prepared in the same manner as in Example 4 except that the absorbent was used, and the occult blood concentration of human urine was prepared in the same manner as in Example 4 except that the absorbent was used. The degree was measured.

Using the absorbent of the comparative example, the occult blood concentration measured by the measurement method of Example 4 and the occult blood concentration measured by the urine test strip were the same in eight samples, but the dry feeling was inferior to that of the absorbent material of the example. It turned out. The results are shown in Table 4-2.

Table 4 _ 2

Further, the absorbents of the examples and the comparative examples were allowed to stand at 50 ° C. for one month after being prepared, and then the same occult blood measurement was performed as described above. The results are shown in Tables 4-13. Table 4 1 3

From Table 4-3, it can be seen that the absorbent of the present invention has a small change over time even after one month and can be measured sufficiently, whereas the absorbent of the comparative example can be sufficiently measured at the time of preparation, but after one month, However, it was found that the measurement could not be performed due to severe daily changes. Example 5 [Absorptive sheet for antigen measurement according to fifth embodiment: measurement of / 32-microglobulin in human urine]

Preparation of absorbent sheet:

FIG. 7 is a cross-sectional view of a part of the absorbent sheet of Example 5; Is as follows.

Surface film (OF) (hereinafter also referred to as (OF)): Polyethylene terephthalate nonwoven fabric (15 cm x 45 cm) with a thickness of Ι Ι Ο μηι.

Water-absorbent resin layer (AL) (hereinafter also referred to as (AL)): 12 g of Aquapearl DS-150 T and 12 g of cotton wool of Japanese Pharmacopoeia (Kawamoto Sangyo Co., Ltd.) are homogeneous in appearance. The mixture was defibrated by hand approximately 500 times while mixing. After that, the defibrated mixture was spread to a size of 15 cm x 45 cm, and then heated using a heater plate press (Model No. N 408 — 0 0) manufactured by NPA System Co., Ltd. Pressed at 8 ° C. for 30 seconds at 8 kg Z cm ² .

Labeled antibody carrying layer (B1) (hereinafter referred to as (B1)): Filter paper (No.2, manufactured by Advantech, cut into 5 mm x 5 mm) After impregnation (25 ° C, 30 minutes) with the phosphate buffer solution of y)), filter paper was taken out and dried (25 ° C, 2 hours, 1755 mmHg). The concentration of (My) in the phosphate buffer was 300 μg Zm1, pH 7, 0, containing 0.1% bovine serum albumin.

Labeled antibody (My): Peroxidase-labeled anti-32-microglobulin polyclonal antibody.

My was prepared using anti-] 32-microglobulin polyclonal antibody (manufactured by Dako Japan) and horseradish peroxidase (manufactured by Toyobo Co., Ltd.). 'Ishika, et al .; J. Biochem, Vol. 92 (1992) 14 14 13 14 24].

BZF separation layer (C 1) (hereinafter also referred to as (C 1)): A membrane filter (mixed cellulose ester type, pore size 0.3 μππ, diameter 47 mm, manufactured by Advantech) is used as antigen β2 — A solution of microglobulin (manufactured by SIPAC) in a phosphate buffer (antigen concentration 5; Ug Zml, pH 7, 0.1% bovine serum (Including Bumin) at 25 ° C for 1 hour, then take out the sheet and dry (3 hours at 25 ° C and 1 755 mmHg).

Coloring agent layer (D) (hereinafter also referred to as (D)): Filter paper (N 0.2, manufactured by Advantech, cut to 2 Omm in diameter) One sheet of o-trimethyl After immersion in a ethanol solution (0.1% (wZw)) at 25 ° C for 5 minutes, the filter paper was taken out and dried (25 ° C, —75 mmHg for 30 minutes). .

Back film (U F) (also referred to as (U F)): Polypropylene finolem (15 cm X 45 cm) with a thickness of 40 μπι.

Using the above constituent materials, processing was performed as follows to produce an absorbent sheet.

First, (AL) is placed under OF, (B1) and (C1) are placed in this order so that it is located at the center of (AL), and a hole with a diameter of 20 mm is placed at the center. After placing the (UF) with the open, place the (D) in this hole, and then apply double-sided tape with a width of 5 mm along the circumference. UF).

The sides of the above layers (15 cm x 45 cm quadrilateral) were hot-pressed with a width of 1 cm each to obtain a diagnostic absorber.

Color-forming aid solution: A solution in which phosphoric acid-monocitrate buffer (pH 5.0) contains 0.02% by weight of hydrogen peroxide.

Tested samples: Twenty samples of human urine were obtained immediately before the test.

Creating Standard Urine: artificial urine (urea 2 0 .0 g, N a C 1 8 .0 g, M g SO 4 · 6 H 2 O 0 .8 g, C a C l 2 '2 H 2 O 0. Β-microglobulin was added to 0 g, 0 g, 100 g, 100 g, and 100 g, respectively. , 140, 160, 180, and 200 ng / m 1.

Test method : The sample (80 ml) was placed in a 200 ml beaker and allowed to flow down at a speed of 5 ml Z seconds while gradually tilting the beaker so that the mouth was in contact with the absorbent sheet.

Five minutes after the entire amount was allowed to flow, the UF was peeled off, and a coloring aid solution was applied to (D) to form a sheet after coloring. The above procedure was performed on 20 human urines. In addition, a similar test was performed for standard urine to perform a color reaction, and a color sheet after standard color development corresponding to the concentration of 32-microglobulin was prepared. Visually compare the color intensity of the colored sheet obtained from the sample with the color intensity of the standard colored sheet, and determine the / 32-microglobulin concentration of the closest standard colored sheet in the sample] 3 2 — Microglobulin concentration was used. Table 5 shows the measurement results of the concentration of [32-microglopurin using the absorbent sheet of the present invention]. Conventional method for measuring 32—microglobulin concentration:

Measurement of the concentration of [32] -microglobulin using 20 samples of human urine used in Example 5 using darazym β2- -icrog 1 obu 1 in — EIATEST (released by Wako Pure Chemical Industries) Did.

The measurement procedure is as follows.

Dilute the sample 11 times with buffer (all reagents are included in the kit), add 20 μl to a test tube, put one antibody bead, and allow to stand at 37 ° C for 1 hour. Then, the reaction solution was removed with an aspirator. Wash twice with 3 ml buffer (injection → shake → aspirate), transfer the antibody beads to a new test tube, add 500 μl of substrate coloring solution, and add 37 ° C. C and left to warm for 30 minutes. 3 ml of the enzyme reaction stop solution was added, mixed well, and the absorbance was measured at 492 nm. Separately, the absorbance was measured in the same manner as above using a standard antigen solution, and a calibration curve was prepared. Then, the concentration of the sample was calculated. Table 5 shows the measured values of β 2 -microglobulin concentration by the conventional method. Note that, in each case, the concentration of 32-microglobulin is 10

If it was 0 ng / m 1 or less, it was judged to be normal urine, and if it exceeded 100 ng / m 1, it was judged to be urine-rich (probably abnormal).

Table 5 shows the results.

Table 5

Good correlation (correlation coefficient = 0.91) was observed between the measurement results obtained by the method of the present invention and the conventional method. Example 6 [Absorptive sheet for antigen measurement according to fifth embodiment: measurement of J32-microglobulin in human serum]

The same absorbent sheet for diagnosis and color-forming aid liquid as in Example 5 were used. Preparation of standard serum:

] 32-Microglobulin was added to physiological saline to prepare a standard serum having a concentration of 0.01 µg / m 1 in a concentration range of 0 to 0.22 / z g / m 1. Test sample:

Twenty samples of human serum were obtained. The test was performed by diluting serum 20-fold with phosphate buffer (0.02 M, pH 7.0) as a test sample. Test method:

The measurement was performed in the same manner as in Example 5 except that the standard serum was used instead of the standard urine, and the test sample described above was used instead of the urine as the sample. The concentration of] 32-microglobulin of the test sample obtained from the color intensity evaluation of the sheet after color development was multiplied by 20 to obtain the | 32-microglobulin concentration of the original sample. Conventional method for measuring i32—microglobulin concentration:

For 20 samples of the above human serum (before dilution with buffer), use Darazyme; 32—Microg 1 obulin—EIATEST (Distributor: Wako Pure Chemical Industries) 2—Microglobulin Was measured.

Table 6 shows the measurement results obtained by the method using the absorbent sheet of the present invention and the conventional method.

Incidentally, three 2-mic Roguropuri emission concentration in any of the methods 1. 8 £ Roh ml determined following value, if the normal serum, 1. 8 mu _{If § Ζηι} 1 Ru beyond the high-containing serum (abnormal There is a high possibility). Table 6

Good correlation (correlation coefficient = 0.88) was observed between the measured value of the / 32-microglobulin concentration between the method of the present invention and the conventional method. Industrial applicability

Diagnosis using the absorbent sheet or the absorbent article for diagnosis of the present invention allows the above-mentioned changes in the physical condition of animals and humans, the presence or absence of pregnancy, the presence or absence of disease, and the progress of the disease to be confirmed. The target diseases and changes in physical condition include cancer (stomach cancer, colorectal cancer, lung cancer, liver cancer, thyroid cancer, breast cancer, uterine cancer, urinary tract cancer, ovarian cancer, prostate cancer, etc.) , Infectious diseases, steroid therapy, renal urinary tract tumors, asymptomatic bacteriosis, antibiotic administration and hormonal abnormalities.

Claims

The scope of the claims

1. Water-absorbent resin (A) and a diagnostic agent containing the (M), animal diagnostic absorber characterized that you also have water absorbing capacity of 6 0 times with the water-absorbing resin (A) is small ₍

2. It contains a water-absorbent resin (A) and a diagnostic agent (M). The water-absorbent resin (A) is an amide of an aliphatic unsaturated monocarboxylic acid, an alkylene oxide additive, or an alkylated product thereof. And at least one monomer (a1) selected from the group consisting of alkyl and etherified compounds as a constituent monomer, and determined by neutralization titration at pH 4 to 9 A diagnostic absorbent having an acid or base equivalent of Z g or less.

3. Water-absorbing resin (A) is used to form one or more monomers (a 2) selected from the group consisting of unsaturated aionic monomers, unsaturated thione monomers and salts thereof. 3. The absorbent according to claim 2, which is contained as a constituent monomer.

4. The absorbent according to claim 2, wherein the water-absorbent resin (A) further contains 0.01 to 3 mol% of the crosslinkable monomer (b).

5. The water-absorbent resin (A) is 90 to 99 moles of the monomer (a1). /. , For the monomer (a2) :! 4. The absorbent material according to claim 3, wherein the absorbent material comprises from 0.1 to 10 mol%, and from 0.01 to 3 mol% of the crosslinking monomer (b).

6. the least even 4 0% by weight of the water absorbent resin (A), the made water absorbing resin (A) weight based Dzui on 2 5 _1! 1 to 2.5% of the diagnostic agent (M), The diagnostic agent (M) is supported on the support or coated with a water-soluble or water-degradable coating material, and the water-absorbent resin (A) is mixed with the supported or coated diagnostic agent (M). Become a diagnostic absorber.

7. The diagnostic agent (M) is selected from the group consisting of a pH diagnostic agent (Ml), a protein diagnostic agent (M2), a glucose diagnostic agent (M3), and an occult blood diagnostic agent (M4) 7. The absorbent according to claim 1, wherein the absorbent is one or more types.

8. Diagnostic absorbent containing water-absorbent resin (A) and diagnostic reagent for antigen-antibody reaction (M 0).

9. A diagnostic agent comprising an antigen-antibody reaction diagnostic agent (M 0), a labeled antibody (My) or a labeling antigen (M x), and, if necessary, a coloring agent (d). Absorbent according to item 8.

10. The absorbent material according to claim 2, wherein the water-absorbent resin (A) has at least 60 times the water-absorbing ability.

1 1. The absorbent material according to any one of claims 1, 2, 6, and 8, which is in the form of granules or aggregates.

1 2. As the constituent material, the absorbent material according to any one of claims 1, 2, 6, and 8, and at least one selected from the group consisting of fiber, nonwoven fabric, paper, and synthetic resin film Diagnostic sheet.

1 3. (1) water-absorbent resin layer (AL) and layer (B 1) carrying labeled antibody (My) or labeled antigen (M x); or

(2) The layer (B 2) carrying the labeled antibody (My) or the labeled antigen (M x) on the water-absorbent resin (A)

B / F separation layer (C), a diagnostic absorbent sheet having:

14. The absorbent sheet according to claim 13, wherein the water permeation rate of the BZF separation layer (C) at 25 ° C and 1 atm is 0.0 :! to 3 mLZ.

1 5. Paper mummets, toiles for pets, simple toys, sanitary napkins, incontinent patches, made from the absorbent sheet according to claim 12 or 13. Or wound sheet.