JPH11202213A - Sample holder for microscope - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a conveniently usable sample holder which can surely hold a petri dish of a different diameter and can easily hold slide glass or a sample wider than the slide glass can be easily held. SOLUTION: In the sample holder for a microscope provided with a holding part for holding a petri dish 70 or slide glass attached on the stage of the microscope and placed on a holder substrate 20, the holding part is composed of a petri dish holding part 30 for holding the petri dish 70 and a slide glass holding part for holding the slide glass. Further, the petri dish holding part 30 is moved in respect to the holder substrate 20 to make the petri dish 70 different in size possible to be held and the slide glass holding part is composed of step parts 21a and 21b provided on the holder substrate 20.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a specimen holder for a microscope.

[0002]

2. Description of the Related Art Specimens to be observed with a microscope, especially specimens to be observed with an inverted microscope, include circular petri dishes having various diameters, a rectangular slide glass, a hemocytometer wider than the slide glass, and a so-called Terasaki plate. .

[0003] Since there are various types of specimens having different shapes as described above, conventionally, a user prepares a plurality of specimen holders corresponding to each specimen type and prepares a corresponding specimen holder each time the specimen type is changed. It had to be replaced, which was inconvenient. As a sample holder which solves this problem, a sample holder capable of holding various types of samples disclosed in Japanese Patent Publication No. Sho 62-23843 is known.

FIG. 7 is a plan view of a sample holder of a conventional microscope.

The specimen holder includes a holder substrate 120 and a pair of holding portions 131 provided on the holder substrate 120.
132.

The holding portions 131 and 132 are provided on the holder substrate 120.
Strips 121, 122 in the direction approaching or moving away from each other
And can move along the strips 123 and 124. The Petri dishes 1 are placed on the holding portions 131 and 132 at positions facing each other.
V-shaped steps 131a, 132a for holding the
Are formed.

Therefore, by moving the holding portions 131 and 132, the V-shaped step portions 131a and 132a
Thus, petri dishes 170 of various diameters can be held from the diameter direction.

Also, two notches 131b, 13b are formed at the edges 131e, 132e of the V-shaped steps 131a, 132a.
1c and notches 132b and 132c are formed, respectively, so that these 131b and 131c and notch 132
The slide glass (not shown) can be sandwiched and held from the longitudinal direction by b and 132c.

Further, a click mechanism is constituted by the projections 133a and 134a provided on the holder substrate 120 and the spring wires 133b and 134b provided on the holding portions 131 and 132.

[0010] The holding mechanism 131, which corresponds to the slide glass whose size is determined by the click mechanism, is determined by this click mechanism.
132 is performed.

Further, recesses 131d and 132d for holding a petri dish having a small diameter are formed in the edges 131e and 132e of the V-shaped steps 131a and 132a.

[0012]

However, when holding the slide glass, it is necessary to move the holding portions 131 and 132 so that the projections 133a and 134a are dropped into the click portions of the spring wires 133b and 134b.
It is necessary to carefully move the holding units 131 and 132.

Further, since the holding portions 131 and 132 move except when the projections 133a and 134a are completely dropped into the click portions of the spring wires 133b and 134b, reliable holding cannot be performed depending on the diameter of the petri dish 170. There is a problem that happens.

Further, in order to secure the recesses 131d and 132d for holding a petri dish with a small diameter, the cut radii of the V-shaped steps 131a and 132a, the notches 131b and 131c for holding the slide glass, and the like. Notch 13
There is a problem that it is necessary to reduce 2b and 132c and it becomes difficult to process.

SUMMARY OF THE INVENTION The present invention has been made in view of such circumstances, and an object thereof is to surely hold petri dishes having different diameters and to easily hold a slide glass or a specimen wider than the slide glass. The object of the present invention is to provide a microscope sample holder that can be easily used.

[0016]

According to a first aspect of the present invention, there is provided a holding means for holding a petri dish or a slide glass mounted on a stage of a microscope and mounted on a holder substrate. In the sample holder of the microscope, the holding means is composed of a petri dish holding means for holding the petri dish and a slide glass holding means for holding the slide glass, and the petri dish holding means moves with respect to the holder substrate and has different sizes. Wherein the slide glass holding means is a step provided on the holder substrate.

The holding means comprises a petri dish holding means for holding a petri dish and a slide glass holding means for holding a slide glass. The petri dish holding means can move with respect to the holder substrate and hold petri dishes of different sizes. Since the slide glass holding means is a step provided on the holder substrate, a circular petri dish is held by the petri dish holding means, and a rectangular slide is held by the step. Further, the petri dish holding means is not affected by the shape of the slide glass holding means.

According to a second aspect of the present invention, in the sample holder of the microscope according to the first aspect, the petri dish holding means moves in a direction orthogonal to a longitudinal direction of the slide glass held by the sample holder. It is characterized by.

The petri dish holding means moves in a direction perpendicular to the longitudinal direction of the slide glass held by the specimen holder, so that the operating range of the holding means can be narrowed.

According to a third aspect of the present invention, in the sample holder of the microscope according to the first aspect, the dimension of the step portion in the moving direction of the petri dish holding means is larger than the dimension of the slide glass in the transverse direction, and The glass holding means includes an adjusting mechanism for adjusting the dimensional difference.

The size of the step in the moving direction of the Petri dish holding means is larger than the dimension in the short direction of the slide glass, and the slide glass holding means has an adjusting mechanism for adjusting the dimensional difference. Can easily hold not only the slide glass but also the one in which the size of the dish holding means moving direction is larger than the slide glass.

According to a fourth aspect of the present invention, in the sample holder of the microscope according to the third aspect, the adjusting mechanism is a rotatable claw provided on the step portion.

Since the adjusting mechanism is a rotatable claw provided on the step portion, it is possible to adjust a dimensional difference in the moving direction of the petri dish holding means with a simple configuration.

According to a fifth aspect of the present invention, in the sample holder of the microscope according to the first aspect, a scale indicating a diameter of the petri dish is provided on the holder substrate, and a pointer of the scale is provided on the petri dish holding means. It is characterized by having.

A scale indicating the diameter of the petri dish is provided on the holder substrate, and a scale pointer is provided on the petri dish holding means. If the diameter of the petri dish is measured in advance, the pointer of the petri dish holding means is set to the diameter of the petri dish. Can be moved to the graduation position corresponding to.

[0026]

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is a plan view showing a sample holder of a microscope according to an embodiment of the present invention mounted on a stage of an inverted microscope.

On the fixed stage 11 of the microscope main body 10, a movable portion 12 movable in the XY directions is provided. A sample holder 15 for holding a sample such as a slide glass 60 is fixed to the movable portion 12 via a leaf spring 13. The specimen holder 15 is provided with a screw 1 from the movable part 12.
It is possible to remove it by loosening 4.

The sample holder 15 can be moved by a stage handle (not shown) provided on the movable part 12.

FIG. 2 is an enlarged view of the specimen holder for explaining a holding state of the petri dish, and FIG. 3 is a sectional view taken along the line III-III of FIG.

The specimen holder 15 includes a holder substrate 20 and a Petri dish holding section (Petri dish holding means) 30.

Steps (slide glass holding means) 21a, 21 on which both ends of the slide glass 60 (see FIG. 1) are placed are placed on the holder substrate 20 having an opening formed in the center.
b is formed.

The steps 21a and 21b are composed of two rectangular portions having different longitudinal dimensions. Rotatable claws (adjustment mechanisms) 22a and 22b are provided at both ends in the longitudinal direction of the rectangular portion having a large longitudinal dimension. Claws 22a,
22b has a substantially elliptical shape, and each has a shaft 23 at one end.
a and 23b.

The holder substrate 20 is provided with a step 24 for holding the petri dish 70 in a direction perpendicular to the longitudinal direction of the slide glass 60 (moving direction of the petri dish holding means indicated by an arrow a). ing.

The Petri dish holding unit 30 is a slide glass 60
The petri dish holding portion 30 is formed with a step portion 31 facing the step portion 24.

The steps 24 and 31 are formed with two curved surfaces having different curvatures. Large radius of curvature R1 is 32.5m
m, and the small radius of curvature R2 is 15 mm.

On the outer periphery of the step portions 24 and 31, a step portion 25 having a predetermined width serving as an escape of a lid (not shown) of the petri dish 70 is provided.
32 are formed. The petri dish 70 can be held by the steps 25 and 32 with the lid kept on, so that the lid is not lifted up by the holder substrate 20 and comes off.

At both ends of the petri dish holding portion 30, fixing nuts 33a and 33b are provided. At one end of the petri dish holding portion 30, a pointer 34 of a scale (described later) 27 indicating the diameter of the petri dish 70 is provided.

The nuts 33a and 33b are provided with the holder substrate 20.
The screws 35a, 3 are extended through guide grooves 26a, 26b extending in the short direction of the slide glass 60 of FIG.
5b is screwed. The head of the screw is machined at both ends of the circular shape to have the same width as the guide grooves 26a and 26b.
a, 26b, but cannot rotate.

The petri dish 7 extends along one guide groove 26a.
A scale 27 indicating a diameter of 0 is provided.

When observing with a petri dish 70,
The petri dish holding section 30 is moved while one of the petri dishes 70 (the lower side of the petri dish 70 in FIG. 2) is held by the step portion 24.

After positioning the step portion 31 of the Petri dish holding portion 30 in contact with the other side of the Petri dish (the upper side of the Petri dish 70 in FIG. 2), the nuts 33a and 33b are tightened to put the Petri dish holding portion 30 on the holder substrate 20. Fix it.

At this time, if the diameter of the petri dish 70 is measured in advance, the pointer 34 of the petri dish holding portion 30 can be adjusted to the scale 27 of the holder substrate 20 in advance.

FIG. 4 is an enlarged view of the specimen holder for explaining the holding state of the slide glass.

Incidentally, the standard size of a standard slide glass for a microscope is determined to be 76 mm × 26 mm (tolerance: ± 0.5 mm). Therefore, the dimension between the side surface 28a of the step portion 21a and the side surface 28b of the step portion 21b is 77 m, which is slightly larger than the longitudinal dimension of the standard slide glass.
m.

The size of the step portions 21 a and 21 b in the direction of movement of the petri dish holding portion is formed larger than the size of the slide glass 60 in the short direction.

When the observation with the slide glass 60 is performed after the observation with the petri dish 70, first, the guide grooves 26a and 26b are provided so that the slide glass 60 can be easily mounted.
Up to the upper limit (the position shown in FIG. 4)
To move.

After that, the claws 22 a and 22 b are rotated by 90 ° to be parallel to the longitudinal direction of the slide glass 60.

At this time, the side surfaces 29a of the claws 22a, 22b
And the side surface 29b of the steps 21a, 21b is 27 mm.
It is formed so that it becomes.

Therefore, the side surfaces 29 of the claws 22a, 22b
a, a space (77 mm × 27 mm) surrounded by the side surface 29b of the step portions 21a and 21b, the side surface 28a of the step portion 21a, and the side surface 28b of the step portion 21b is formed, and the slide glass 60 is easily mounted on the step portions 21a and 21b. Can be placed. The width of the nail is 10 mm.

FIG. 5 is an enlarged view of a sample holder for explaining a holding state of a sample whose lateral dimension is larger than the slide glass.

As a specimen whose lateral dimension is larger than the slide glass 60, for example, there is a hemocytometer 80.

The hemocytometer 80 is formed by forming a groove 81 having a width of about 2 mm and a depth of about 0.1 mm on a rectangular glass plate such as a slide glass 60.

The hemocytometer 80 adheres a cover glass 83 so as to cover the groove 81, and
2. Used to count the number of red blood cells, white blood cells, etc.

The size of the hemocytometer 80 is 76 mm ×
It is 26 mm to 76 mm x 35 mm.

By the way, as shown in FIG.
By rotating 2b by 90 °, the steps 21a and 21b are wider by the width (10 mm) of the claws 22a and 22b.
A space of mm × 37 mm is secured.

Therefore, the hemocytometer 80 is moved to the step 21
a, 21b can be easily held.

The holder substrate 20 has steps 21a, 2a
Steps 41a and 41b having a space (81.5 mm × 56 mm) wider than 1b are 1 m away from steps 21a and 21b.
It is formed at a position higher by m.

By using the steps 41a and 41b, a so-called terasaki plate (not shown) having a size of 80 mm × 55 mm can be held.

When switching from observation using the petri dish 70 to observation using a terasaki plate (for example, a 60-hole terasaki plate), the petri dish holding portion 30 is moved to the guide groove 2 in the same manner as when switching to observation using the slide glass 50.
After moving to the upper limit position of 6a, 26b, the terasaki plate may be placed and held on the steps 41a, 41b.

According to the above embodiment, the following effects can be obtained.

Various types of petri dishes 70 having different diameters can be held only by moving the petri dish holding section 30.

The Petri dish holding section 30 has a slide glass 60
Is movable in a direction orthogonal to the longitudinal direction of the plate, so that the operating range of the petri dish holding unit 30 can be narrowed as compared with the conventional configuration.

The steps 24 and 31 have a large curvature (radius of curvature R1 = 32.5 mm) and a small curvature (radius of curvature R2 = 1).
5 mm), the two can be used properly according to the diameter of the petri dish 70, and the petri dish 70 can be easily held.

The rotatable claws 22 are provided on the step portions 21a and 21b.
Since the claw 22a and the bail 22b are provided, the claw 22a and 22b are rotated by 90.degree. to easily hold the hemocytometer 80, which is larger in the moving direction of the dish holding portion than the slide glass 60, like the slide glass. be able to.

Since the claws 22a and 22b are used, the slide glass 60 can always be placed at the same position, and the dimensional difference can be adjusted with a simple configuration.

The movement of the Petri dish holder 30 is controlled by the grooves 26a, 2a.
Since only the screws 35a and 35b are moved along 6b, the switching to the holding of the slide glass 60 can be easily performed, and since there is no click mechanism unlike the conventional example, the slide glass can be securely held. Can be held.

If the diameter of the petri dish 70 is measured in advance, the petri dish holding means 30 can be moved to the position of the scale 27 corresponding to the diameter of the petri dish 70 in advance. And the observation efficiency is improved.

FIG. 6 is a sectional view of a holder substrate for explaining a modification of the holding section.

In this modification, elastic bodies 36a and 36b such as springs and wave washers are interposed between the nuts 33a and 33b and the Petri dish holding portion 30.

According to this modification, the Petri dish holding section 30
Is constantly pressed against the holder substrate 20 with a predetermined force by the elastic bodies 36a and 36b. By appropriately adjusting the elastic force of the elastic bodies 36a and 36b, a suitable resistance can be given to the movement of the Petri dish holding portion 30, and the Petri dish holding portion 30 can be easily stopped at a predetermined position of the holder substrate 20. .

The shapes of the step portions 24 and 31 do not necessarily have to be curved surfaces, and may be V-shaped, for example, as long as the petri dish 70 can be held.

[0073]

As described above, according to the first aspect of the present invention, even if the circular petri dishes have different diameters, the petri dishes are securely held by the petri dish holding means, and the rectangular slide glass is formed on the holder substrate. Since it is held by the step portion independent from the Petri dish holding means, it is excellent in usability. Further, since the petri dish holding means is not affected by the shape of the slide glass holding means, no extra processing cost is required.

According to the second aspect of the invention, the operating range of the holding means can be narrowed as compared with the conventional example. According to the third aspect of the present invention, even if the step portion has a larger size in the moving direction of the Petri dish holding means than the slide glass, the slide portion can be held at the same position by adjusting the adjusting mechanism. Can be held.

According to the fourth aspect of the invention, it is possible to adjust the dimensional difference in the moving direction of the Petri dish holding means with a simple configuration.

According to the fifth aspect of the present invention, if the diameter of the petri dish is measured in advance, the pointer of the petri dish holding means can be moved to the scale position corresponding to the diameter of the petri dish. It can be immediately held on the holder substrate.

[Brief description of the drawings]

FIG. 1 is a plan view showing a state in which a sample holder of a microscope according to an embodiment of the present invention is mounted on a stage of an inverted microscope.

FIG. 2 is an enlarged view of a specimen holder for explaining a holding state of a petri dish.

FIG. 3 is a sectional view taken along line III-III in FIG. 2;

FIG. 4 is an enlarged view of a specimen holder for explaining a holding state of a slide glass.

FIG. 5 is an enlarged view of a sample holder for explaining a holding state of a sample whose lateral dimension is larger than a slide glass.

FIG. 6 is a cross-sectional view of a holder substrate illustrating a modified example of the holding unit.

FIG. 7 is a plan view of a sample holder of a conventional microscope.

[Explanation of symbols]

 Reference Signs List 11 Stage 20 Holder substrate 21a, 21b Step (slide glass holding means) 22a, 22b Claw (adjustment mechanism) 27 Scale 30 Petri dish holding part (Petri dish holding means) 34 Hand 60 Slide glass 70 Petri dish

Claims (5)

[Claims] 1. A sample holder for a microscope, comprising a holding means for holding a petri dish or a slide glass mounted on a stage of a microscope and mounted on a holder substrate, wherein the holding means comprises: a petri dish holding means for holding the petri dish; And a slide glass holding means for holding the slide glass, wherein the petri dish holding means is movable with respect to the holder substrate and can hold petri dishes of different sizes, and the slide glass holding means is provided on the holder substrate. A specimen holder for a microscope, wherein the specimen holder is a stepped part. 2. The microscope sample holder according to claim 1, wherein the petri dish holding means moves in a direction orthogonal to a longitudinal direction of a slide glass held by the sample holder. 3. The method according to claim 1, wherein a dimension of the step portion in a moving direction of the petri dish holding means is larger than a dimension of the slide glass in a transverse direction, and the slide glass holding means includes an adjusting mechanism for adjusting the dimensional difference. A specimen holder for a microscope according to claim 1, characterized in that: 4. The microscope sample holder according to claim 3, wherein the adjusting mechanism is a rotatable claw provided on the step portion. 5. The microscope sample holder according to claim 1, wherein a scale indicating the diameter of the petri dish is provided on the holder substrate, and a pointer for the scale is provided on the petri dish holding means.