KR100443641B1 - DNA stealth pin manufacture method - Google Patents

Abstract

The present invention relates to a method for producing a DNA impregnating pin that takes a DNA sample and takes a certain volume. A body forming a first curved surface and a first flat surface curved at a predetermined radius of curvature with the first curved surface to maintain a predetermined distance at the edge of the DNA impregnation pin, and a second adhesive surface facing the body; A process of separating and processing the pin member forming the second curved surface and the second flat surface, and a fine gap is formed between the first flat surface and the second flat surface, and the pair of first curved surface and the first (2) an assembling process of assembling the second bonding surface of the pin member against the first bonding surface of the body so that the curved surface can accommodate the DNA sample, and the first bonding surface and the second bonding adhered to each other. For welding so that the face can be fixed It is made of a contact process, so that the micro-gaps formed at the edge of the DNA impregnation pin can be finely adjusted using a pin member assembled to the body, thereby improving the precision of the DNA impregnation pin.

Description

DNA impregnation pin manufacturing method {DNA stealth pin manufacture method}

The present invention relates to a method for producing a DNA impregnated pin, and more particularly to a method for producing a DNA impregnated pin for precisely processing a DNA impregnated pin taking a predetermined volume of DNA sample using a durable material. It is about.

Recently, in the field of genetics and protein research, the use of microarrays in which DNA samples are arranged in order is exploding.

The microarray is a mediator that binds known or unknown genes based on the base binding law, and provides a mediator that automates the process of uncovering unknown genes. The microarray is on a glass or nylon nylon. Thousands of spots are made by DNA impregnating pins on the membrane, and the probe can be used to search for complementary binding using known probes. .

The DNA impregnation pin is an ultra-precision component taking a DNA sample and taking a predetermined volume. Referring to FIG. 1, a conventional DNA impregnation pin is as follows.

Referring to FIG. 1, in the conventional DNA impregnation pin, a pair of pin members 11a and 11b are integrally formed, and the pair of pin members 11a and 11b form the entry and exit portions 12 facing each other. In addition, the receiving part 12 forms a receiving part 13 which is bent at a predetermined radius of curvature.

The entrance and exit portion 12 is compressed from the outside to the inside of the pair of pin members (11a, 11b) to adjust the micro-gap of the entry and exit portion 12, the receiving portion 13 to the impregnation amount of the DNA sample Therefore, it can be produced in various forms, such as straight, streamlined.

Therefore, the DNA sample is introduced through the entry and exit 12 formed by the pair of pin members 11a and 11b, and the DNA sample introduced through the entry and exit 12 is a receiving portion 13 having a predetermined radius of curvature. Volume).

Subsequently, when the DNA impregnated pin, in which the DNA sample is deposited, is moved and taken on a glass plate or a nylon membrane, the DNA sample deposited on the DNA impregnated pin flows out to form a spot.

However, as described above, the conventional DNA impregnating pin compresses a pair of pin members 11a and 11b to adjust the gap between the entry and exit portions 12, so that the gap between the entry and exit portions 12 cannot be finely adjusted and thus the precision is low. Has a problem.

In addition, since the pair of pin members 11a and 11b are compressed to adjust the gap between the entry and exit portions 12, a metal material having a high hardness cannot be used, and thus, the wear resistance of the DNA impregnation pin is inferior.

The present invention has been made in view of the above-described problems, and is intended to improve the precision of DNA impregnated pins by making it possible to finely adjust the interval of the DNA impregnated pins, which can volume the target DNA sample.

Another aim is to minimize surface deformation and morphological changes due to the repeated use of DNA impregnated pins by enabling the use of durable materials.

1 is a perspective view showing a conventional DNA impregnation pin.

Figure 2 is an exploded perspective view showing the DNA impregnation pin of the present invention.

Fig. 3 is an assembly view showing the DNA impregnation pin of the present invention. Fig. 4 is a front view of the DNA impregnation pin of the present invention.

* Description of the symbols for the main parts of the drawings *

11a, 11b: pin member 12: entry and exit portion

13, 50: receiving portion 20: body

21: first adhesive surface 23: the first curved surface

25: first flat surface 30: pin member

31: second bonding surface 33: second bending surface

35: second flat surface 40: fine spacing

Method for producing a DNA impregnated pin of the present invention for achieving the above object is configured as follows.

The first adhesive surface bonded by welding, the first curved surface and the first curved surface is bent to a predetermined radius of curvature to the volume of the DNA sample, and the first curved surface is bent to the constant radius of curvature and the DNA impregnation pin A processing step of separating and processing a body forming a first flat surface having a constant spacing at an edge, and a pin member forming a second adhesive surface, a second curved surface, and a second flat surface so as to face the body; ,

A second gap is formed between the first flat surface and the second flat surface so as to be spaced apart from each other, and the pair of first curved surfaces and the second curved surfaces are provided with an accommodating portion capable of depositing a DNA sample. An assembling process of assembling the surface by bringing it into close contact with the first adhesive surface of the body;

Characterized in that the welding process for welding so that the first adhesive surface and the second adhesive surface which are in close contact with each other can be fixed.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Figure 2 is an exploded perspective view showing a DNA impregnation pin of the present invention.

Fig. 3 is an assembly view showing the DNA impregnation pin of the present invention. Fig. 4 is a front view of the DNA impregnation pin of the present invention.

2 to 3, the method of manufacturing the DNA impregnated pin of the present invention is processed to separate the body 20 and the pin member 30 welded to the body 20 so that the DNA sample volume Process, and an assembly process of assembling the body 10 and the pin member 30 processed in the processing process, and a welding process of welding the body 20 and the pin member 30 assembled in the assembly process. .

The body 20 to be processed in the processing step is a first adhesive surface 21 which is bonded by welding, the first bending surface and the first bending surface to be bent at a predetermined radius of curvature with the first adhesive surface 21 ( 23) and a first flat surface 25 that is bent at a predetermined curvature radius with the first curved surface 23 to form a predetermined gap at the edge of the DNA impregnated pin is formed, the pin member 30 is the body The first adhesive surface 21, the first curved surface 23, and the first flat surface 25 formed on the surface 20 of the second adhesive surface 31 and the second curved surface 33 to face each other. The second flat surface 35 is formed.

In the assembling process, when the pin member 30 is brought into contact with the body 20, the pair of first adhesive surfaces 21 and the second adhesive surface 31 are in close contact with each other, and the pair of first curved surfaces 33 are in contact with each other. ) And the second curved surface 33 are matched to the volume of the DNA sample, and are assembled so as to face each other so that a minute gap is formed between the first flat surface 25 and the second flat surface 35.

The welding process welds the first adhesive surface 21 and the second adhesive surface 31 of the body 20 and the pin member 30 which are in contact with each other to be fixed.

Therefore, when the body 20 and the pin member 30 of the DNA impregnated pin processed as described above are assembled to face each other and welded, the first flat surface 25 and the second flat surface at the edge of the DNA impregnated pin. As the 35 is spaced by a predetermined interval, the micro-gap 40 is formed, the DNA sample is extracted through the micro-gap 40, the receiving port that can volume the DNA sample introduced through the micro-gap. 50 is formed in a predetermined space by the first curved surface 23 and the second curved surface 33.

The fine interval 40 is preferably 1/100 [mm], the error range is ± 3/1000 ~ 1/1000, the shape of the receiving port 50 can be formed in various ways depending on the volume. .

Looking at the operation and effects of the present invention as described above are as follows.

A first adhesive surface 21 adhered by welding, a first curved surface 23 that is bent to a predetermined radius of curvature with the first adhesive surface 21, and a first curved surface 23 for depositing a DNA sample, and the first curved surface 23 ) And a body 20 to be bent at a constant radius of curvature to form a first flat surface 25 that maintains a constant distance to the edge of the DNA impregnation pin, and the second adhesive surface 21 to face the body 20. ), The second curved surface 33, and the pin member 30 forming the second flat surface 35 is separated and processed.

Therefore, the DNA impregnation pin can be manufactured using a metal material having excellent durability, such as cemented carbide, by separating and processing the body 20 and the pin member 30 to manufacture the DNA impregnation pin.

Thereafter, a fine gap 40 is formed between the first flat surface 25 and the second flat surface 35, and the pair of first curved surfaces 23 and the second curved surfaces 33 are DNA samples. The second adhesive surface 31 of the pin member 30 is assembled to the first adhesive surface 21 of the body 20 so as to form a receiving opening 40 capable of forming a volume, and then the first adhesive surface is assembled. (21) and the second adhesive surface 31 are welded to produce a DNA impregnation pin.

Therefore, the micro-gap 40 formed between the first flat surface 25 and the second flat surface 35 can be finely adjusted according to the movement of the pin member 30, so that the micro-interval on the DNA impregnated pin 40 can be formed precisely.

As described above, the present invention can use a metal material having high durability by separating the body and the pin member to produce a DNA impregnation pin, and by using a metal material having high durability, Reduces deformation or shape change

In addition, the fine spacing formed at the edge of the DNA impregnation pin can be finely adjusted using a pin member assembled to the body, thereby improving the precision of the DNA impregnation pin.

Claims (1)

A first adhesive surface 21 adhered by welding, a first curved surface 23 that is bent to a predetermined radius of curvature with the first adhesive surface 21, and a first curved surface 23 for depositing a DNA sample, and the first curved surface 23 ) And a body 20 to be bent at a constant radius of curvature to form a first flat surface 25 that maintains a constant distance to the edge of the DNA impregnation pin, and the second adhesive surface 31 to face the body 20. ), The process of separating and processing the pin member 30 forming the second curved surface 33, the second flat surface 35, Between the first flat surface 25 and the second flat surface 35 so that the micro-gap 40 is spaced apart, between the pair of first curved surface 23 and the second curved surface 33 Assembling process for forming the receiving portion 50 to the volume of the DNA sample is formed in the second adhesive surface 31 of the pin member 30 in close contact with the first adhesive surface 21 of the body 20 and, DNA impregnated pin manufacturing method comprising a welding process for welding so that the first and second contact surfaces 21 and 31 are in close contact with each other can be fixed.