JPH10509322A - 核酸変異体および病原体の迅速な検出および同定 - Google Patents

核酸変異体および病原体の迅速な検出および同定

Info

Publication number
JPH10509322A
JPH10509322A JP8516227A JP51622796A JPH10509322A JP H10509322 A JPH10509322 A JP H10509322A JP 8516227 A JP8516227 A JP 8516227A JP 51622796 A JP51622796 A JP 51622796A JP H10509322 A JPH10509322 A JP H10509322A
Authority
JP
Japan
Prior art keywords
cleavage
nucleic acid
dna
stranded
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP8516227A
Other languages
English (en)
Japanese (ja)
Inventor
イー． ダールバーグ，ジェームス
アイ． リャミチェフ，ヴィクター
アン ディー． ブロー，メアリー
シー． オールデンバーグ，メアリー
エム． ハイスラー，ローラ
フォーズ，ランス
マイケル オリーヴ，デイヴィッド
Original Assignee
サード ウェーヴ テクノロジーズ，インコーポレーテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/484,956 external-priority patent/US5843654A/en
Priority claimed from US08/520,946 external-priority patent/US6372424B1/en
Application filed by サード ウェーヴ テクノロジーズ，インコーポレーテッド filed Critical サード ウェーヴ テクノロジーズ，インコーポレーテッド
Publication of JPH10509322A publication Critical patent/JPH10509322A/ja
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP8516227A 1994-11-09 1995-11-09 核酸変異体および病原体の迅速な検出および同定 Ceased JPH10509322A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US33716494A 1994-11-09 1994-11-09
US08/337,164 1994-11-09
US40260195A 1995-03-09 1995-03-09
US08/402,601 1995-03-09
US08/484,956 US5843654A (en) 1992-12-07 1995-06-07 Rapid detection of mutations in the p53 gene
US08/484,956 1995-06-07
US08/520,946 1995-08-30
US08/520,946 US6372424B1 (en) 1995-08-30 1995-08-30 Rapid detection and identification of pathogens
PCT/US1995/014673 WO1996015267A1 (fr) 1994-11-09 1995-11-09 Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes

Publications (1)

Publication Number Publication Date
JPH10509322A true JPH10509322A (ja) 1998-09-14

Family

ID=27502560

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8516227A Ceased JPH10509322A (ja) 1994-11-09 1995-11-09 核酸変異体および病原体の迅速な検出および同定

Country Status (5)

Country Link
EP (1) EP0788557A4 (fr)
JP (1) JPH10509322A (fr)
AU (2) AU4234796A (fr)
CA (1) CA2203627C (fr)
WO (1) WO1996015267A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5922575A (en) * 1994-04-18 1999-07-13 Mayo Foundation For Medical Education & Research Mutations in the katG gene useful for detection of M. tuberculosis
CA2243353C (fr) * 1996-01-24 2010-03-30 Third Wave Technologies, Inc. Clivage invasif d'acides nucleiques
US6562611B1 (en) * 1996-11-29 2003-05-13 Third Wave Technologies, Ins. FEN-1 endonucleases, mixtures and cleavage methods
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
WO2001098537A2 (fr) 2000-06-17 2001-12-27 Third Wave Technologies, Inc. Sites d'hybridation accessibles a l'acide nucleique
AU2001282860A1 (en) * 2000-07-19 2002-02-05 Pharmacia And Upjohn Company Staphylococcus aureus ribosomal protein s20, corresponding gene and methods for the identification of antibacterial substances
EP3172337B1 (fr) * 2014-07-23 2020-04-01 CONGEN Biotechnologie GmbH Procédé pour la détection de sepsis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3179481B2 (ja) * 1990-06-27 2001-06-25 プリンストン ユニバーシティ 突然変異体ｐ５３検出用プローブ
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法

Also Published As

Publication number Publication date
CA2203627A1 (fr) 1996-05-23
WO1996015267A1 (fr) 1996-05-23
EP0788557A4 (fr) 2003-08-06
EP0788557A1 (fr) 1997-08-13
CA2203627C (fr) 2000-06-06
AU4444699A (en) 1999-10-14
AU4234796A (en) 1996-06-06

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