CA2203627C - Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes - Google Patents

Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes Download PDF

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Publication number
CA2203627C
CA2203627C CA 2203627 CA2203627A CA2203627C CA 2203627 C CA2203627 C CA 2203627C CA 2203627 CA2203627 CA 2203627 CA 2203627 A CA2203627 A CA 2203627A CA 2203627 C CA2203627 C CA 2203627C
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CA
Canada
Prior art keywords
nucleic acid
cleavage
substrate
stranded
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 2203627
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English (en)
Other versions
CA2203627A1 (fr
Inventor
James E. Dahlberg
Victor I. Lyamichev
Mary Ann D. Brow
Mary C. Oldenburg
Laura M. Heisler
Lance Fors
David Michael Olive
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Third Wave Technologies Inc
Original Assignee
Third Wave Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/484,956 external-priority patent/US5843654A/en
Priority claimed from US08/520,946 external-priority patent/US6372424B1/en
Application filed by Third Wave Technologies Inc filed Critical Third Wave Technologies Inc
Publication of CA2203627A1 publication Critical patent/CA2203627A1/fr
Application granted granted Critical
Publication of CA2203627C publication Critical patent/CA2203627C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur des moyens de segmentation de structures de clivage d'acide nucléique d'une manière propre à un site. On utilise des enzymes, dont des 5' nucléases et des 3' exonucléases, pour dépister les mutations connues et inconnues y compris les mutations de la base unique des acides nucléiques. L'invention porte également sur des méthodes d'identification dans des échantillons de mutation génétiques dans des séquences de gènes humains y compris le gène humain p53, et sur des méthodes de détection et d'identification dans des échantillons d'espèces et d'agents pathogènes bactériens et viraux.
CA 2203627 1994-11-09 1995-11-09 Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes Expired - Lifetime CA2203627C (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US33716494A 1994-11-09 1994-11-09
US08/337,164 1994-11-09
US40260195A 1995-03-09 1995-03-09
US08/402,601 1995-03-09
US08/484,956 1995-06-07
US08/484,956 US5843654A (en) 1992-12-07 1995-06-07 Rapid detection of mutations in the p53 gene
US08/520,946 US6372424B1 (en) 1995-08-30 1995-08-30 Rapid detection and identification of pathogens
US08/520,946 1995-08-30
PCT/US1995/014673 WO1996015267A1 (fr) 1994-11-09 1995-11-09 Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes

Publications (2)

Publication Number Publication Date
CA2203627A1 CA2203627A1 (fr) 1996-05-23
CA2203627C true CA2203627C (fr) 2000-06-06

Family

ID=27502560

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2203627 Expired - Lifetime CA2203627C (fr) 1994-11-09 1995-11-09 Detection et identification rapides de variants de l'acide nucleique et d'agents pathogenes

Country Status (5)

Country Link
EP (1) EP0788557A4 (fr)
JP (1) JPH10509322A (fr)
AU (2) AU4234796A (fr)
CA (1) CA2203627C (fr)
WO (1) WO1996015267A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5922575A (en) * 1994-04-18 1999-07-13 Mayo Foundation For Medical Education & Research Mutations in the katG gene useful for detection of M. tuberculosis
DK1634890T3 (da) * 1996-01-24 2009-03-09 Third Wave Tech Inc Invasiv klövning af nukleinsyrer
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
EP0966542B1 (fr) * 1996-11-29 2008-11-12 Third Wave Technologies, Inc. Agents de clivage ameliores
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
US7060436B2 (en) 2000-06-17 2006-06-13 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
WO2002008265A2 (fr) * 2000-07-19 2002-01-31 Pharmacia & Upjohn Company Sequence nucleotidique complete du gene de la proteine ribosomale de staphylococcus aureus, s20, et methodes d'identification de substances antibacteriennes
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法
US20170204452A1 (en) * 2014-07-23 2017-07-20 Steffen Mergemeier Method for the detection of sepsis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2064555A1 (fr) * 1990-06-27 1991-12-28 Arnold J. Levine Sondes pour detecter p53 mutant
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase

Also Published As

Publication number Publication date
AU4444699A (en) 1999-10-14
CA2203627A1 (fr) 1996-05-23
AU4234796A (en) 1996-06-06
EP0788557A4 (fr) 2003-08-06
EP0788557A1 (fr) 1997-08-13
JPH10509322A (ja) 1998-09-14
WO1996015267A1 (fr) 1996-05-23

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Effective date: 20151109