JPH10313610A - Seed immersion adjusting agent and seed processed by the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a means for more easily imparting a desired property such as the useless umproductive growth prevention or the like of the seedlings of rice plants to plants. SOLUTION: Paclobutrazol is used as the effective component of this seed immersion adjusting agent capable of imparting the desired property to the seed by simply immersing the seed in an aqueous solution containing the effective component. The paclobutrazole is used so as to bring the aqueous solution containing the paclobutrazole and the seed into contact, then dry it and produce a plant body provided with a fixed property.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to the use of pactbutrazole as an active ingredient of a seed immersion regulator capable of imparting desired properties to a seed simply by immersing the seed in an aqueous solution containing the active ingredient. Seed immersion adjuster, use of pactbutrazole to produce a plant having certain properties by contacting the seeds with an aqueous solution containing pactbutrazole and drying, and the seed immersion adjuster and the above It is an invention belonging to the technical field related to seeds and plants having desired properties, which are produced through the use of pactbutrazole. Further, the present invention is an invention which is preferably applied particularly when the seed in the above invention is rice seed or wheat seed.

[0002]

2. Description of the Related Art Conventionally, attempts have been made to improve the yield of most cultivated plants by spraying pesticides, plant hormones and the like for various purposes during the growth process. . However, such application of pesticides,
Since the soil is exhausted, the surrounding environment is degraded, and the safety of the harvest itself is also raised, it is preferable to use as little as possible. In response to such demands, that is, in order to keep the application rate of pesticides low and to secure the yield, in recent years, attempts have been made to incorporate specific genes into plant genes to produce plants having specific properties. It has been done.

[0003]

One of the cases particularly problematic when cultivating cultivated plants is the raising of rice seedlings. In other words, seedling raising is the most important work in paddy rice cultivation, and it is said that it is half-cropping or seven-cropping. In particular, sapling seedlings resulting from high temperature and high humidity have poor survival and growth after transplantation, and great care and effort have been devoted to nurturing healthy seedlings. In order to control the length of the rice seedlings, it is necessary to control the temperature in the house, etc., according to the germination and the growing season from seeding to transplanting. It takes a considerable number of days for raising seedlings because of the necessity of avoidance), and it is necessary to minimize irrigation in order to prevent breeding. Various considerations are required, such as performing a heat retention process to avoid low-temperature damage.

[0004] However, with the recent lack of successors and an increase in the scale of planting, a simpler method of raising seedlings (the conventional method is said to limit planting to 5 ha per person) is desired. In order to satisfy this demand, creating the above-described transgenic plant is also considered as one means, and it cannot be denied that the utility of this transgenic plant is certainly excellent. It is general that a great deal of labor is required to produce a transgenic plant.

[0005] That is, the problem to be solved by the present invention is to provide a means for more easily imparting the above-mentioned desired properties such as the prevention of rice seedling growth to plants.

[0006]

Means for Solving the Problems The present inventor has made intensive studies to solve this problem. As a result, by pre-treating seeds (paddy) with pactbutrazole, which is a plant growth regulator, before raising the seedling, it is excellent in cold resistance, appearance is suppressed in length, and the leaf age is advanced, We found that so-called "stubby seedlings" can be raised easily and in a short number of days.

[0007] In particular, according to this technique, in rice seedlings and wheat seedlings, low-temperature tolerance is easily and quickly imparted without strict temperature control and water control, and the length of the seeds is suppressed, and healthy seedlings are advanced. The inventors have found that seedlings can be raised, and completed the present invention. That is, the present inventor provides the following invention in the present application.

[0008] In the first aspect, there is provided a seed immersion adjuster comprising pactbutrazole as an active ingredient.

The plant according to claim 3, wherein the seeds are brought into contact with an aqueous solution containing pactbutrazole, and from which the seeds are dried, the plant length is suppressed, the cold resistance is imparted, and the leaf age is further advanced. Provide the use of pactbutrazole to produce

[0010] The present invention provides a seed according to claim 5, wherein the seed is brought into contact with an aqueous solution containing pactbutrazole and then dried.

[0011] In claim 7, the seeds are brought into contact with an aqueous solution containing pactbutrazole, and then the dried seeds are germinated. Provide the plant.

Further, in claim 2, claim 4, claim 6 and claim 8, the use of the seed soaking conditioner (claim 2), pactbutrazole (claim 4), The invention provides that the seed in the seed (claim 6) and the seed in the plant body (claim 8) are rice paddy or wheat seed, respectively.

In the present invention, "seed immersion regulator"
The expression means the use of a specific component that can impart desired properties to the seed by simply immersing the seed in an aqueous solution containing the active ingredient.

[0014]

Embodiments of the present invention will be described below. The present invention, by contacting an aqueous solution containing paclobutrazol with the seeds of the plant, to prevent the growth of the plant, impart cold resistance, and can further promote leaf age, the technical idea of that This is the invention based on the basic axis.

Paclobutrazol has the chemical name (2
RS-3RS) -1- (4-chlorophenyl) -4,4
-Dimethyl-2- (1H-1,2,4-triazole-
1-yl) pentan-3-ol, a triazole-based plant growth regulator represented by the following chemical formula and already registered in 21 countries including Japan.

[0016]

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[0017] Pactoptrazole has a main effect of inhibiting the biosynthesis of gibberellin in plants, is absorbed from the roots and foliage of the plant, translocates apically in the conduit, and is transferred to various parts of the plant. It is known to be carried.

As a typical action of this pactbutrazole, a dwarfing action is known. That is, it is known that administration of pactobutrazol to a plant causes internode shortening, reduction of leaf area, thickening of leaf, deep greening of leaf, and increase of chlorophyll content in the plant. ing.

The present invention has been completed by finding that this very useful action of pactbutrazole as a dwarfing agent can be exerted on a plant just by bringing it into contact with a seed before germination in an aqueous solution. It is an invention. As will be described later, the present invention makes it possible to dramatically reduce the labor required in the process of raising plants.

At present, as pesticides containing pactbutrazole as an active ingredient, Trimid Flowable (Nippon Agrochemical Co., Ltd., etc.), Bonsai Flowable (Nippon Agrochemical Co., Ltd., Zeneca Co., Ltd., etc.), Smarect Granules (Japan Agricultural Chemicals Co., Ltd., Zeneca Co., Ltd.), Boundy Flowable (Zeneca Co., Ltd., Takeda Pharmaceutical Co., Ltd., etc.), Boundy Granules (Zeneca Co., Ltd., Takeda Pharmaceutical Co., Ltd., etc.)
And the like.

In the present invention, this aqueous solution containing paclobutrazol is subjected to a dipping step of bringing the aqueous solution into contact with the seeds of the plant, thereby preventing the plant from growing, providing cold resistance, and further increasing the leaf age. Let it proceed.

The concentration of pactoptrazole in this aqueous solution varies depending on the type of plant seeds to which the effect is imparted. For example, in the case of rice paddy or wheat seed, the concentration is 0.01% by weight or more, 1% or more, based on the whole aqueous solution. 0.0% by weight or less, preferably 0.02% by weight or more and 0.1% by weight or less.

When the amount is less than 0.01% by weight based on the total amount of the aqueous solution, it is difficult and unfavorable to sufficiently provide the desired effect of preventing rice growth at least in rice seeds or wheat seeds. If the content is more than 10% by weight, the dwarfing effect becomes remarkable, the workability at the time of transplantation becomes poor, and the germination becomes uneven, and the subsequent growth of the plant is not preferred.

When a commercial product containing paclobutrazol as an active ingredient is used, the amount of the commercial product in water is determined according to the concentration of paclobutrazol in the product.

The temperature of the aqueous solution in the immersion step is set at 0 ° C. to 30 ° C., preferably 5 ° C. to 20 ° C. for rice seed or wheat seed. If the temperature is lower than 0 ° C, the aqueous solution freezes, which is not preferable.
It is not preferable to exceed the value, since the uniformity of the effect on individual seeds is reduced.

Under these conditions, the seeds are immersed in the above aqueous solution of paclobutrazol for about 12 hours or more, and in the case of rice or wheat seeds, preferably for 16 hours to 24 hours, and then dried. By doing so, seeds having desired properties can be obtained.

It is possible to use the seeds immersed as described above as they are. However, in order to more effectively exert the effect of contacting pactbutrazole in the aqueous solution with the seeds, the above-described drying method is used. Processing is effective. In addition, the effect of the immersion treatment in the aqueous solution of pactbutrazole was found to last for at least one year after the drying, and it was possible to store the batch-treated seeds until they were sold to each farmer. Can also be stored up to the seedling raising operation, which brings about an advantage that the seedling raising operation can be started at a desired time.

The specific form of the drying treatment is not limited, and any commonly known drying means such as sun drying and hot air drying can be selected. However, these drying treatments are usually performed at 25 ° C. or less, preferably 20 ° C. or less, for the purpose of making the effect on the seeds after the immersion treatment as uniform as possible.

By germinating and growing the seed thus prepared under normal conditions or a wider range of temperature conditions and irrigation conditions, the seeds are dwarfed than plants grown from normal untreated seeds. , The leaf age is advanced, and a plant to which cold resistance has been imparted can be grown. Furthermore, in plants germinated from these seeds, growth at normal and high temperatures is prevented, and healthy plants are easily produced. In particular, in rice paddy, the number of days for raising seedling is reduced from 2/3 to 1/2 of the conventional value. It can be shortened and the occurrence of stuffy seedlings can be suppressed.

The seeds of plants applicable to the present invention include a wide range of seed plants in general, including the rice paddy or wheat seed (spring wheat, autumn wheat, etc.). Specifically, for example, barley seeds,
Examples include sugar beet seeds, corn seeds such as sweet corn and dent corn, but are not limited to these seeds. Also, it does not matter the variety of each plant.

As described above, in the present invention, by contacting an aqueous solution containing paclobutrazol with the seed of a plant, the plant is prevented from growing and is given cold resistance, and the leaf age is further advanced. be able to.

That is, as described above, the present invention provides the use of pactbutrazole as a "seed immersion control agent", and also provides the "use of pactbutrazole" in the above steps. Is what you do. The present invention also provides a seed obtained by the above steps and a plant obtained by germinating the seed.

In the seed immersion control agent of the present invention,
Auxiliary components such as drugs other than pactoptrazole can be added as long as the intended effects of the present invention are not impaired. For example, it is possible and effective to add a surfactant such as Tween 20 as an auxiliary component for the purpose of improving the solubility and spreadability of pactbutrazole. It is also possible to add other agents that do not essentially affect the effects of the present invention, for example, a bactericide as an auxiliary component for disinfecting seeds before raising seedlings.

Further, a commercially available agricultural chemical in which the above-mentioned auxiliary components are previously blended with pactbutrazole can be used for preparing the seed immersion regulator of the present invention.

According to the present invention, the following ripple effects can be expected. 1. Agricultural Effects According to the present invention, it is possible to grow healthy plants that have been given firm resistance to low temperatures after transplantation and have been grown. That is, even if these plants, especially seedlings, encounter an abnormally low temperature, it is possible to prevent a decrease in yield. Furthermore, since the plant body according to the present invention does not prolong even under long-term normal temperature or short-term high temperature, the temperature in the house can be kept higher than that of the conventional method. For example, in the case of rice, as described above, this leads not only to a reduction in the number of days for raising seedlings and suppression of stuffy seedlings, but also to a great reduction in the labor of careful house management as in the past.

2. Economic effects The yield of paddy rice nationwide in Japan is about 12 million tons, and the gross production value is about 1 trillion yen. The ability to stably grow good seedlings in the seedlings that are important for this production will have a very large economic effect (1% increase in yield affects 10 billion gross production value). Furthermore, when the labor involved in raising seedlings is converted into monetary value, there is a great economic effect.

[0037]

The present invention will be described more specifically with reference to the following examples, which should not limit the technical scope of the present invention. In these examples, "%" indicating the blending amount represents% by weight based on the total amount unless otherwise specified.

[Example 1] Examples relating to rice (1) Production of seed of the present invention Rice paddy (variety: Kirara 397) was used as pactbutrazole at 0.04% by weight, 0.1% by weight and 0.2% by weight. It was immersed in an aqueous solution containing 15% by weight at 15 ° C. for 18 hours (the weight ratio of rice paddy and treatment liquid at the time of immersion was rice paddy: treatment liquid = 1: 2). After the completion of the immersion, the rice paddy was air-dried for 5 days to produce a rice paddy subjected to the immersion treatment according to the present invention.

(2) Laboratory test Dwarfing effect: The rice immersed rice paddy was placed on a petri dish covered with filter paper and grown at 25 ° C. with appropriate addition of water. The leaf height after 11 days of growth is shown in FIG. 1. As is clear from this figure, in all the test systems in which pactbutrazole and rice paddy were brought into contact with each other in an aqueous solution, the pactbutrazole content was reduced. A concentration-dependent dwarfing effect was clearly observed. In the figures, "PB" represents a group treated with pactoptrazole, and "DW" represents a group treated with distilled water (common in all figures).

Low temperature tolerance test: Seedlings grown from the above-immersed rice seeds and seedlings grown from untreated rice seeds were placed in a dark place at 25 ° C. for 11 days, and then kept at a low temperature of 5 ° C. Each was subjected to low temperature stress for 8 days. These seedlings were exposed to the light at 25 ° C for 10 days,
The degree of damage (0 (small) to 5 (large)) of each seedling was examined. FIG. 2 shows the results of comparison of the degree of failure after the low-temperature treatment between the treated section and the non-treated section.

In FIG. 2, the damage due to the low temperature in the 0.04% section of pactbutrazole is smallest,
Next, in the 0.1% section, the damage was clearly reduced. However, in the 0.2% ward,
The effect of reducing the low temperature damage was hardly recognized. From this, the concentration of bactoptrazole in the aqueous solution is from 0.degree.
It turned out that about 04% by weight was appropriate.

(3) Outdoor seedling raising test In actual rice seedling raising in a farmhouse, disinfection, immersion, germination [herein "immersion" means germination (germination) before seeding rice seedlings in a seedling box. In order to maintain the uniformity, the rice paddy is immersed in water, which is different from the “immersion step” in the present invention. ] Is usually performed. Therefore, seedlings were actually bred and tested for the effect of the present invention. In this test, the rice immersed rice pad obtained in the above (1) was used.

The rice paddy was disinfected using a commercially available disinfectant (Techlead C Flowable, Kumiai Chemical Industry Co., Ltd.) according to a conventional method. The immersion treatment was performed at 15 ° C. for 5 days.
Germination was performed at 33 ° C. for 24 hours. Then, using a commercially available seedling raising soil and a seedling raising box, seeding was carried out at a rate of one grain per one trout, and germination and seedling raising were carried out in a greenhouse maintained at about 25 ° C. The survey was performed 16 days after sowing.

Examination of dwarfing effect: Table 1 shows the results of examining the dwarfing effect.

[Table 1]

As a result, it has been clarified that the dwarfing effect is exhibited without being impaired even after the above-mentioned treatments such as disinfection, immersion and germination. The effect of promoting leaf age was also observed.

Examination of Improvement of Low Temperature Resistance Table 2 shows the results of the examination of low temperature resistance. The low-temperature treatment in the low-temperature tolerance test was performed by exposing the seedlings in each test section at 5 ° C. for 10 days. Whether or not low-temperature tolerance was imparted was determined by determining the ratio of these seedlings to the total number of dead seedlings (dead rate) when the seedlings were left at 25 ° C. for 10 days.

[0047]

[Table 2]

As a result, even in a real environment,
It became clear that low temperature stress tolerance was given to rice. Also, from the viewpoint of imparting particularly low temperature resistance to rice paddy, the concentration of bactoptrazole in the aqueous solution was found to be about 0.04% by weight in this case as well.

Example 2 Example on Wheat (1) Production of Seeds of the Present Invention Seeds of wheat (variety: Chihoku) were used as pactbutrazole at 0.04% by weight, 0.1% by weight and 0.2% by weight. Immersed in an aqueous solution containing 20% by weight at 15 ° C. for 18 hours (the weight ratio of wheat seed to treatment liquid at the time of immersion was wheat seed: treatment liquid =
1: 2). After completion of the immersion, the wheat seeds were air-dried for 5 days to produce immersion-treated wheat seeds according to the present invention.

(2) Laboratory test Dwarfing effect: The immersed wheat seeds were placed on a petri dish covered with filter paper, and grown at 25 ° C. with appropriate addition of water. The leaf height after 13 days of growth is shown in FIG. 3, and as is clear from this figure, in all the test systems in which pactbutrazole and wheat seeds were brought into contact in an aqueous solution,
A dwarfing effect depending on the concentration of pactbutrazole was clearly observed.

Test for improving low-temperature resistance: Wheat is inherently resistant to relatively low temperatures, so that it is unlikely to be damaged by low-temperature stress at 5 ° C. like rice. Therefore, in this test, a more severe freezing test was performed. That is,
Wheat in the test plot [Autumn wheat (Chihoku wheat), Spring wheat (Haruyutaka and Spring Akebono)]
Were placed at 5 ° C. for 30 days, treated at −30 ° C. for 3 minutes, returned to a light place at 25 ° C., and examined for the degree of damage to the stems and leaves of each wheat.

FIG. 4 shows the degree of obstacle after freezing [the damage degree (the same index as in FIG. 2 above, but the maximum value of the damage degree shown in this table is 4). . ] Was compared in the pactbutrazole-treated group and the untreated group. As a result, it was clarified that in each of the wheats, the low temperature injury in the foliage was clearly light in the pactobutrazol-treated group, and the low temperature tolerance was imparted to the soaked wheat seeds.

FIG. 5 shows the growth recovery ability of each wheat after freezing (expressed as the ratio of the number of surviving seedlings to the number of seedlings used in the test system) (−30 ° C.).
After treatment at 25 ° C for 4 days after the low temperature treatment, some survived in the wheat treated with pactobutrazol, but almost all died in the control (No. The graph on the left side of FIG. 5 shows the results in the test system where the low-temperature treatment at −30 ° C. was performed for 4 minutes, and the graph on the right side shows the results in the test system where the same low-temperature treatment was performed for 5 minutes.)

[0054]

According to the present invention, seed immersion using pactbutrazole as an active ingredient of a seed immersion control agent capable of imparting desired properties to the seed simply by immersing the seed in an aqueous solution containing the active ingredient is provided. Conditioner, use of pactbutrazole to make a plant having certain properties by contacting an aqueous solution containing pactbutrazole with seeds and then drying the seeds, and the above-described seed soak conditioner and pactob Provided are seeds and plants having desired properties, which are produced through the use of tolazole.

[Brief description of the drawings]

FIG. 1 is a drawing in which the relationship between the treatment of rice paddy with pactbutrazole and the dwarfing effect on young seedlings was examined.

FIG. 2 is a drawing for examining the relationship between the treatment of rice paddy with pactbutrazole and the effect of imparting low-temperature tolerance to young seedlings.

FIG. 3 is a drawing for studying dwarfing of young seedlings by treatment of seeds of white-spotted wheat with pactobutrazol.

FIG. 4 is a drawing for examining the freezing tolerance of wheat seedlings treated with pactbutrazole.

FIG. 5 is a drawing for examining freezing tolerance of seedlings of wheat seeds treated with pactbutrazole.

Claims (8)

[Claims] 1. A seed immersion adjuster comprising pactbutrazole as an active ingredient. 2. The seed immersion regulator according to claim 1, wherein the seed is rice paddy or wheat seed. 3. After contacting the seed with an aqueous solution containing pactbutrazole, a plant is produced from the dried seed, in which the growth is suppressed, the cold resistance is imparted, and the leaf age is further advanced. Use of pactbutrazole for 4. The use of pactbutrazole according to claim 3, wherein the seed is rice paddy or wheat seed. 5. A seed obtained by bringing a seed into contact with an aqueous solution containing pactbutrazole and then drying. 6. The seed according to claim 5, wherein the seed is rice seed or wheat seed. 7. A plant body in which the seeds are brought into contact with an aqueous solution containing pactbutrazole and then germinated, and the germination of the seeds is suppressed. . 8. The plant according to claim 7, wherein the seed is rice paddy or wheat seed.