JPH10311829A - Portable analyzer for human or animal humor and card type disposable inspecting tool used therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To precisely and quickly transmit the measurement result of an inspection when an urgent patient is transferred by an emergency ambulance to a hospital or the like, and perform a proper diagnosis or treatment in emergency by providing a printing means capable of printing a data on an analyzer itself. SOLUTION: A card type disposable inspecting tool is inserted to the insert port 30 of a portable analyzer to calibrate and measure a sensor 11c. Thereafter, the nozzle of an injector containing the collected blood from a patient is fitted to the injection port of the inspecting tool to inject the blood into the internal passage. Even if the injecting pressure of the blood is dispersed because the passage has a relatively narrow part on the upstream side of the contact part with the sensor 11c and a bundled part on the downstream side, the injected blood is stably carried, and each sensor 11c can stably and precisely detect a desired inspection value. An arithmetic processing part 31 arithmetically operates the current value or voltage value detected by each sensor 11c and outputs data such as various gas concentrations, which are then stored in a memory part 32 and displayed on a display part 33. The data are also printed out by a printer 34.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a portable analyzer which can be used for analyzing gas components in human or animal body fluids, such as blood, and for measuring pH, and a card-type disposable test device used for the analyzer. is there.

[0002]

2. Description of the Related Art An analysis system of this kind is used for examining the cardiopulmonary function of a patient in a hospital or the like, for example, for the acidity PH of blood, the partial pressure of carbon dioxide PCO ₂ , the partial pressure of oxygen PO ₂ , sodium Na, potassium K, and so on. Chlorine Cl, ionized calcium C
a, urea nitrogen BUN, glucose Glu, hematocrit Hct, bicarbonate ion HCO ₃ ⁻ , oxygen saturation SO ₂ ,
Total carbon dioxide TCO ₂ , excess BE, hemoglobin H
It is used to measure the concentration of various gases and ions such as gb, and various stationary and portable types have been proposed and put to practical use. In particular, as a prior art of a portable type, for example, as described in Japanese Patent Publication No. 8-20398, at least one sensor is provided in a housing,
The collected liquid sample is held in the housing orifice, the liquid reagent contained in the housing is passed through the sensor, the sensor is calibrated and measured, and then the liquid sample held inside is passed through the sensor. There is known a system comprising a disposable sensing device adapted to perform a required measurement, and a reader capable of inserting the sensing device, reading the detected data from the sensing device, processing the detected data, and displaying the processed data on a display device. I have. According to this system, required measurement can be performed with a small amount of sample.

[0003]

In such an analysis system comprising a disposable detector and a reader according to the prior art, a small amount of sample is dropped on an orifice provided in the disposable detector and held until measurement. Because of the configuration, the sample touches the atmosphere when the sample is dropped, the gas component to be measured in the sample is affected, and accurate measurement cannot be performed, and the amount of sample that can be introduced into the detection device is very small. Since blood may coagulate quickly, it is necessary to insert the detector into the reader and measure relatively quickly after introducing the sample into the detector. Also, especially in an emergency situation such as used in an ambulance, when a small amount of blood is put into the detection device, the blood may overflow or it may not be able to drip properly into a small orifice. There is also a danger of touching the patient's blood.

Also, the space for storing the sample after measurement by the sensor is relatively small, and the amount of sample introduced is limited, and the introduced sample is once held and stored. Because it is configured to measure with a small amount of sample by providing a means to contact the sensor at the time of measurement, the space for storing the sample after measurement with the sensor is relatively narrow, not only the structure is complicated, but also However, it is necessary to make the amount of sample introduced accurately, and there is a problem in handling.

Further, for example, when an emergency patient carries out an ambulance to a hospital or the like and performs measurement with this kind of apparatus, the measurement data must be accurately transmitted to a doctor in charge at the hospital or the like where the patient is carried. However, if the hospital does not have a system that can read the data stored in the measurement system installed in the ambulance, or the ambulance must be immediately dispatched to the next emergency patient In such a case, the paramedics must write down the data from the device and transmit the data to the hospital, and there is a risk that the delivery of the data takes time and a transmission error occurs.

Accordingly, the present invention solves the problems associated with such a conventional portable analyzer so that analysis can be performed quickly and accurately, and the obtained data can be transmitted quickly and accurately. It is intended to provide a portable analyzer for human or animal body fluids. Another object of the present invention is to use the above portable analyzer together with the portable analyzer, the number of parts is small, the structure is simple, the sample can be easily and safely injected, and the contact between the sample and the atmosphere can be avoided as much as possible. Another object of the present invention is to provide a card-type disposable inspection tool capable of obtaining an accurate inspection value.

[0007]

SUMMARY OF THE INVENTION To achieve the first object of the present invention, a portable analyzer according to the present invention is provided with printing means capable of printing data on itself. In order to achieve another object of the present invention, in the card-type disposable test device according to the present invention, a bodily fluid flow path is configured so that a bodily fluid to be tested can be directly injected with an injector such as a syringe, and the injected fluid is injected. The storage space for the stored body fluid is made large, and the amount of the injected body fluid can be easily confirmed. As used herein, the term "body fluid" refers to human or animal blood,
It means intracellular and extracellular fluids including secreted fluids such as urine and saliva and tissue fluids such as bone marrow fluid.

[0008]

According to one embodiment of the present invention, a portable analyzer of human or animal bodily fluids is provided in a portable casing for at least one test of human or animal bodily fluids. An arithmetic processing unit for detecting a value and cooperating with a card-type disposable inspection tool provided with a sensor for generating an output signal corresponding thereto and receiving and processing the output signal from the inspection tool; A storage unit for storing; a display unit for displaying data of a calculation result of the calculation processing unit on an upper surface of the casing; and a printing unit for printing data of a calculation result of the calculation processing unit. It is characterized by.

According to another aspect of the present invention, a card-type disposable test device for human or animal bodily fluid is provided on one inner surface of two substrates which are liquid-tightly overlapped with each other with a partition plate interposed therebetween. Providing a passage through which a body fluid of a human or animal to be examined passes, and a first body fluid storage portion comprising a groove communicating with one end of the passage, communicating with the other end of the passage, and from the inner surface of one of the substrates. An injection hole for bodily fluid penetrating into the outer surface is provided, and at least one sensor for detecting at least one test value of bodily fluid is provided on the inner surface of the other substrate, and each sensor is detected before detecting each test value of bodily fluid. A recess for receiving a reagent container for calibrating the first body fluid, and a second body fluid storage part comprising a groove communicating with the first body fluid storage part on the one substrate via a hole provided in the partition plate. It is installed on one of the substrates through the opening in the plate. The sensors are positioned so as to face the passage, and the terminals of the sensors are arranged so as to be exposed on the surface of the substrate. Further, one of the substrates is provided with an opening through which a pressing means for pressing the reagent container from the outside is provided. In addition, in the concave portion for receiving the reagent container provided on the other substrate, there is provided a piercing means for piercing the reagent container according to the operation of the pressing means and supplying the reagent therein to the passage at a position upstream of each sensor. Features. At least one of the two substrates may include a transparent or translucent portion that allows the bodily fluid in the first or second bodily fluid storage unit to be externally confirmed.
Also preferably, the partition plate is provided with adhesive layers on both sides, and the two substrates can be fixed to each other in a liquid-tight manner via the partition plate.
In addition, the passage for the body fluid of the human or animal to be examined may be provided with a constriction downstream of each sensor. Furthermore, the body fluid injection hole may be formed to taper inward so as to be in close contact with the nozzle portion of the body fluid injector.

[0010]

Embodiments of the present invention will be described below with reference to the accompanying drawings. 1 to 5 show a card-type disposable inspection tool according to an embodiment of the present invention. The illustrated device is made of a suitable material, such as a transparent or translucent plastic material, and is sandwiched between two substrates 1A, 1B, which are superimposed on each other, and a suitable sheet-like material having an adhesive layer on both sides. The two substrates 1A and 1B are joined in a liquid-tight manner by the thin partition plate 2 when assembled.

As shown in FIG. 1, a passage 3 formed of a groove through which a body fluid of a human or animal to be inspected passes is formed on the inner surface of the substrate 1A, that is, the surface facing the thin partition plate 2, as shown in FIG. It is formed in a U-shape along the peripheral edge. One end of the passage 3 communicates with the injection hole 4 for bodily fluid, and the other end of the passage 3 communicates with the first bodily fluid storage unit 5 formed of a groove formed on the inner surface of the substrate 1A, similarly to the passage 3. . As shown in FIG. 2, the body fluid injection hole 4 is slightly tapered from the inner surface to the outer surface of the substrate 1A, and a guide shoulder 4a is provided around the body fluid injection hole 4 on the outer surface of the substrate 1A. It is provided and configured to guide and adhere to the nozzle portion of an injector (not shown) such as a syringe containing the collected bodily fluid, so that bodily fluid can be injected without touching the atmosphere. As shown, the grooves constituting the first body fluid storage unit 5 are arranged in a bent manner, and the width and depth thereof are larger than the width and depth of the grooves constituting the passage 3 in order to obtain a sufficient volume. It is set large. In addition, as shown, the passage 3 continues to a relatively narrow portion 3a extending from the body fluid injection hole 4, a sensor contact portion 3b following the relatively narrow portion 3a, and a sensor contact portion 3b. It has a constricted portion 3c and a portion 3d extending from the constricted portion 3c to the groove of the first bodily fluid reservoir 5. At a portion of the passage 3 adjacent to the relatively narrow portion 3a of the sensor contact portion 3b, a narrow groove 6 forming a part of the reagent passage is formed inward. Further, a branch passage 7 extending toward the peripheral edge portion is formed in the sensor contact portion 3b of the passage 3, and the distal end of the branch passage 7 terminates in a through hole 8. The through hole 8 is positioned so as to face a reference electrode described later, and can be loaded with a gel-like saturated potassium chloride solution. Further, an opening 9 is formed in an inner region of one of the substrates 1A surrounded by the U-shaped passage 3.
The opening 9 is provided for receiving a means for pressing a reagent container described later during measurement. In addition, a through hole 10 for guiding and locking when the substrate 1A is inserted into an analyzer according to the present invention, which will be described later, is provided at a substantially central portion of the substrate 1A.

On the other side of the substrate 1B, a concave surface 12 for mounting the sensor substrate 11 is formed along one corner on the inner surface thereof, that is, the surface facing the thin partition plate 2, and In the position corresponding to the opening 9 in 1A,
As shown in FIG. 3, a mounting concave surface 14 of the reagent container 13 is formed, a round depression 14a is formed at the center of the bottom of the mounting concave surface 14, and the center thereof protrudes inward to protrude inward.
A needle-like body 15 for perforating a hole is integrally provided.
Reference numeral 16 denotes a first body fluid storage unit 5 on one substrate 1A.
5 shows a second body fluid storage unit including a curved groove provided symmetrically at a position corresponding to FIG. In the reagent container 13,
The sensor substrate 11 is filled with a reagent for calibrating each sensor. When calibrating the sensor prior to measurement, the reagent container 13 is pressed against the needle-like body 15 by the reagent container pressing means through the opening 9. It is made of a material that can be easily pierced when pressed, and can be preferably made, for example, as a foil pack. As shown in FIGS. 3 and 4, the reagent passage extends from a round recess 14a at the center of the bottom of the mounting concave surface 14 to a position corresponding to the free end of the narrow groove 6 which forms a part of the reagent passage in one of the substrates 1A. Are formed. The sensor substrate 11 has a plurality of electrodes 11b formed by applying a conductive ink on a substrate 11a. Each electrode 11b has an acidity PH, a partial pressure of carbon dioxide PCO ₂ , and a partial pressure of oxygen
O ₂ , sodium Na, potassium K, chlorine Cl, ionized calcium Ca, urea nitrogen BUN, glucose Gl
u, hematocrit Hct, bicarbonate ion HCO ₃ ⁻ , oxygen saturation SO ₂ , total carbon dioxide TCO ₂ , excess base BE,
A sensor 11c for measuring the concentration of various gases and ions such as hemoglobin Hgb is provided. In the illustrated embodiment, the sensors 11c are arranged so as to be able to measure eight types of inspection values among these inspection values. Each sensor 11
c can use its own existing one and is configured to detect as voltage value or current value,
A description of those details will be omitted. Each electrode 11
b is connected via conductors 11d to terminals 11e arranged along one side edge of the substrate 11a. These terminals 11e connect the two substrates 1A and 1B to each other with the thin partition plate 2 interposed therebetween. It is designed to be exposed when fixed. That is, as can be seen from FIG. 5, the width of one substrate 1A is configured to be slightly smaller than that of the other substrate 1B.
Each of the upper terminals 11e is exposed.

The starting end 16a of the groove constituting the second body fluid storage unit 16 communicates with the terminal end 5a of the first body fluid storage unit 5 in one of the substrates 1A through the communication hole 18 in the partition plate 2. At the end 16b of the groove of the second bodily fluid reservoir 16, a hole 19 is provided for communicating with the outside, and this hole is made of a plug made of a material permeable to air and impermeable to liquid. A member (not shown) is attached, and the body fluid injected at the time of use is supplied to the passage 3.
Flows into the first and second bodily fluid reservoirs through
The body fluid that has flowed in is prevented from leaking outside. The other substrate 1B corresponding to the through hole 10 in one substrate 1A
A through hole 20 is provided in the upper position, and the two substrates 1A, 1
When B are pasted together, these through holes 10, 20 are aligned and aligned.

The partition plate 2 is provided with a plurality of openings 21 at positions along the sensor contact portion 3b of the passage 3 and the branch passage 7 on one of the substrates 1A, and the sensor contact portion 3b of the passage 3 is provided. Body fluid flowing into the branch passage 7
Each sensor 11c and electrode 11b on the other substrate 1B through 21
To be in contact with. Further, the partition plate 2 has a hole 22 provided at a position corresponding to the through hole 10 in one substrate 1A and the through hole 20 on the other substrate 1B,
A, a hole 23 communicating the free end of the narrow groove 6 in A with the free end of the narrow groove 17 on the other substrate 1B, a hole 24 provided at a position corresponding to the body fluid injection hole 4 on one substrate 1A, and one Hole 25 or an easily breakable portion 26 provided at a position corresponding to the opening 9 in the substrate 1A.

FIG. 6 schematically shows an embodiment of a portable analyzer according to the present invention. This portable analyzer has an insertion slot 30 for inserting the card-type disposable test tool illustrated in FIGS.
As shown in FIG. 7, an arithmetic processing unit 31 for arithmetically processing output signals detected by each sensor in the card-type disposable inspection tool.
A storage unit 32 that stores the calculation result of the calculation processing unit 31, a display unit 33 that is arranged on the upper surface of the casing and displays data of the calculation result of the calculation processing unit 31, And a printer 34 for printing data. The operation unit of the apparatus is incorporated in the display unit 33 shown in FIG. 6, and a reagent container pressing means is provided inside the apparatus. The reagent container pressing means is provided by inserting a card-type disposable test tool. , And can be operated.

The operation of the illustrated apparatus configured as described above will be described by taking a patient's blood analysis as an example. First, the prepared card-type disposable test tool is inserted into the insertion port 30 of the portable analyzer to perform calibration measurement of the sensor. That is, by inserting the card-type disposable test device, the measurement item is determined, the reagent container pressing means in the portable analyzer is automatically activated, and the reagent container 13 is passed through the opening 9 of the card-type disposable test device. Is pressed against the needle-like body 15 in the round recess 14a at the center of the bottom of the mounting concave surface 14, whereby the reagent container 13 is torn, and the reagent inside is rounded from the round recess 14a to the narrow groove 17, the partition plate 2 and the like.
Through the hole 23 and the narrow groove 6 in one of the substrates 1A to the upstream end of the sensor contact portion 3b of the passage 3, whereby the calibration of each sensor is performed.

Thereafter, the nozzle of the syringe containing the blood collected from the patient is fitted into the injection hole 4 of the card-type disposable test device, and the blood is injected into the passage 3 inside. At this time, since the passage 3 has a relatively narrow portion 3a on the upstream side of the sensor contact portion 3b and a constricted portion 3c on the downstream side, even if the blood injection pressure varies, the injected blood may Flows slowly and stably along the sensor contact portion 3b of the passage 3, and each sensor can stably and accurately detect a desired test value. Thus, the sensor contact portion 3b of the passage 3
Passes through the constricted portion 3c and the portion 3d and flows into the first body fluid storage portion 5. In this case, in the blood injecting apparatus using a syringe, the amount of blood to be injected can be easily recognized by externally monitoring the state of blood pooling in the first body fluid storage unit 5, so that excessive or insufficient injection is performed. Can be prevented,
Safe and stable measurement is possible. Even if the worker accidentally injects excessive blood and the blood fully accumulates in the first bodily fluid storage unit 5, the second bodily fluid in the other substrate 1B through the communication hole 18 in the partition plate 2 Since it can flow into the reservoir 16, the danger of overflowing blood from the injection hole 4 is avoided. Also, unlike the conventional method in which the injected blood is held in the test tool and the test value of the blood held in the test tool is measured after the calibration operation, the blood injection into the test tool in the present invention is performed. As a result, a blood test value is measured substantially simultaneously, so that an accurate measured value can be obtained.

The current value and the voltage value detected by each sensor 11c are sent to an arithmetic processing unit 31 in the portable analyzer, where they are subjected to arithmetic processing, and the acidity PH and the carbon dioxide partial pressure PCO are calculated.
₂ , oxygen partial pressure PO ₂ , sodium Na, potassium K, chlorine Cl, ionized calcium Ca, urea nitrogen BUN, glucose Glu, hematocrit Hct, bicarbonate ion H
Data relating to various gas concentrations and ion concentrations such as CO ₃ ⁻ , oxygen saturation SO ₂ , total carbon dioxide TCO ₂ , excess base BE, and hemoglobin Hgb are output, and these data are stored in the storage unit 32 on the one hand. At the same time, it is displayed on the display unit 33 arranged on the upper surface of the casing. These data can be printed out by operating the printer 34 if necessary.

In the illustrated embodiment, the two substrates are entirely made of a transparent or translucent material. However, it is sufficient to confirm at least the state in the first and second body fluid storage units. It can also be configured so that it can be seen through. Although the terminals of the sensor are exposed by forming one substrate to be narrower than the other substrate, the two substrates are configured to have the same dimensions so that the terminals of the sensor are exposed on the side surfaces. You may. Further, in the illustrated embodiment, the partition plate is provided with an adhesive layer on both sides, but may be joined using an adhesive instead. Further, the sensor substrate is provided separately from the two main substrates, but may be formed directly on the main substrate 1B instead. Furthermore, the number of sensors can be arbitrarily set according to the number and type of test values.

[0019]

As described above, in the portable analyzer according to the present invention, since the analyzer itself is provided with a printing means capable of printing data, an emergency patient is transferred to a hospital or the like by an ambulance, for example. In this case, the measurement result inspected at this time can be accurately and promptly transmitted to a hospital or the like, and appropriate diagnosis and treatment in an emergency can be performed.

In the card-type disposable test device according to the present invention, the body fluid flow path is configured so that the body fluid to be tested can be directly injected by an injector such as a syringe, so that the body fluid to be tested comes into contact with the atmosphere. Can be avoided, and the inspection value can be accurately detected. In addition, since the reservoir space for the injected body fluid is configured to be large, flexibility with respect to variation in the injection amount of the body fluid to be tested can be increased,
Operability and workability are facilitated. Furthermore, the passage is configured so that the body fluid to be tested flows slowly and stably through the sensor section, so that the sensor can accurately detect the test value even if the injection of the body fluid to be tested is warm or hot. Become. Further, in the card-type disposable inspection tool according to the present invention,
Alternatively, when at least one of the two substrates is provided with a transparent or translucent portion so that the bodily fluid in the second bodily fluid storage unit can be checked from the outside, the state of injection of the bodily fluid into the inside is checked. While performing the injection operation,
As a result, excessive or insufficient injection can be prevented beforehand, and safe and stable operation can be easily performed. Further, in the card-type disposable test device according to the present invention, when the adhesive layers are provided on both surfaces of the partition plate and the two substrates are fixed to each other in a liquid-tight manner via the partition plate, the card-type disposable test device is used. The assembling work of the tool is facilitated, and the manufacturing cost can be reduced. Further, in the card-type disposable test device according to the present invention, when a constricted portion is provided on the downstream side of each sensor in the passage for passing bodily fluid, the flow of bodily fluid passing through the sensor portion can be maintained substantially stationary. As a result, the measurement accuracy of the sensor can be maintained at a high level. Furthermore, in the card-type disposable test device according to the present invention, when the injection hole for the body fluid is formed to taper inward so as to be in close contact with the nozzle portion of the body fluid injector, the body fluid is exposed to the atmosphere when the body fluid is injected. Injection can be performed without any influence, the influence of the atmosphere on the measured values can be avoided, and the reliability of the detection data can be greatly improved.

[Brief description of the drawings]

FIG. 1 is a schematic exploded perspective view showing an embodiment of a card-type disposable inspection tool according to the present invention.

FIG. 2 is a cross-sectional view of a part of one substrate of the card-type disposable inspection tool shown in FIG.

FIG. 3 is an enlarged partial cross-sectional view of a part of the other substrate of the card-type disposable test device shown in FIG.

FIG. 4 is a plan view showing a part of the other substrate part of the card-type disposable test tool shown in FIG. 3;

FIG. 5 is a schematic perspective view showing an assembled state of the card-type disposable inspection tool shown in FIG. 1;

FIG. 6 is a schematic perspective view showing one embodiment of a portable analyzer according to the present invention.

FIG. 7 is a block diagram showing the configuration of the portable analyzer shown in FIG.

[Explanation of symbols]

 1A, 1B: substrate 2: thin partition plate 3: passage composed of a groove for passing bodily fluid 4: injection hole for bodily fluid 5: first bodily fluid reservoir 6: thin groove constituting a part of reagent passage 7: minute Branch passage 8: Through hole 9: Opening 10: Through hole 11: Sensor substrate 11 c: Sensor 11 d: Terminal 12: Concave surface 13: Reagent container 14: Mounting concave surface 15: Needle 16: Second body fluid storage 17: Narrow groove which forms part of reagent passage 18: Communication hole 19: Hole 20: Through hole 21: Multiple openings 22, 23, 24: Hole 25: Hole 26: Fracture part 30: Insertion port 31: Operation processing part 32: Storage unit 33: Display unit 34: Printer

Claims (6)

[Claims] 1. A card-type disposable test device having a sensor for detecting at least one test value of a human or animal body fluid and generating an output signal corresponding thereto in a portable casing. An arithmetic processing unit for receiving and processing the output signal from the lever test device and a storage unit for storing the arithmetic result of the arithmetic processing unit are provided, and the data of the arithmetic result of the arithmetic processing unit is displayed on the upper surface of the casing. A portable analyzer for human or animal bodily fluids, further comprising: a display unit for performing the calculation; and a printing unit for printing data of a calculation result of the calculation processing unit. 2. A passage for passing a body fluid of a human or animal to be inspected, on one inner surface of two substrates which are overlapped with each other in a liquid-tight manner with a partition plate interposed therebetween, and communicates with one end of the passage. A first bodily fluid storage unit comprising a groove, communicating with the other end of the passage, providing an injection hole for bodily fluid penetrating from the inner surface of one substrate to the outer surface, and the inner surface of the other substrate. At least one sensor for detecting at least one test value of bodily fluid, a concave portion for receiving a reagent container for calibrating each sensor before detecting each test value of bodily fluid, and a hole provided in the partition plate. A second bodily fluid storage section comprising a groove communicating with the first bodily fluid storage section of the one substrate, and each of the first and second substrates is arranged so as to face a passage provided in one of the substrates via an opening provided in the partition plate. Position the sensors and connect the terminals of each sensor to the The substrate is exposed to the surface, and further, one of the substrates is provided with an opening through which a pressing means for pressing the reagent container from the outside is provided. A card-type disposable test device for human or animal bodily fluid, comprising a perforating means for perforating a reagent container to supply the reagent therein to the passage at a position upstream of each sensor. 3. The human or animal bodily fluid according to claim 2, wherein at least one of the two substrates has a transparent or translucent portion that allows the bodily fluid in the first or second bodily fluid reservoir to be confirmed from the outside. Card-type disposable inspection tool. 4. The card-type disposable test for human or animal body fluid according to claim 2, wherein the partition plate is provided with adhesive layers on both sides, and the two substrates are fixed to each other in a liquid-tight manner via the partition plate. Utensils. 5. The card-type disposable test device for human or animal bodily fluid according to claim 2, wherein the passage through which the human or animal bodily fluid to be tested is provided has a constricted portion downstream of each sensor. 6. The card-type disposable test device for human or animal body fluid according to claim 2, wherein the body fluid injection hole is formed to taper inward so as to be in close contact with the nozzle portion of the body fluid injector.