JPH10276572A - Method for cultivating mashrooh - Google Patents

Abstract

PROBLEM TO BE SOLVED: To cultivate mashrooms, to improve the form of a sporophore and a harvesting quantity and to obtain high quality mashrooms by seeding mashroom spawns in a medium and raising the sporophores under a specified condition. SOLUTION: The mashroom spawns are seeded in the sterilized medium, the mashroom funguses are predominantly culativated in the medium while preventing the contamination of various germs, the medium where the mashroom spawns are predominantly cultivated is contained in a synthetic resin-made culativating bag where thickness is 10-50 μm and also multiple 0.10-0.45 mm diameter holes are formed in a part which forms in-bag upper space by the proportion of 0.001-0.02% surface opening rate in a state where in-bag upper space corresponding to the one to four-time capacity of the medium is formed, the mashroom funguses are sufficiently cultivated, a stimulation is given to the clutivated mashroom funguses so as to generate the sporophores, the sporophores are raised and, then, the mashrooms are clutivated. Preferably, the medium after harvesting the sporophores is contained in a cultivating bag, the mashroom funguses are sufficiently reculativated, the stimulation is given to the mashroom funguses so as to regenerate the sporophores and the sporophores are raised so as to harvest them.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for cultivating mushrooms. In the cultivation of mushrooms, the inoculum of the mushroom is inoculated into a sterilized medium, and the mushroom fungus is sufficiently cultured in the medium while preventing various bacterial contamination, and then the fully cultured mushroom fungus is stimulated to form fruiting bodies. Develop and harvest the fruiting bodies. Then, with respect to the medium after harvesting the fruiting body, after sufficiently recultivating the mushroom fungi in the medium, the sufficiently recultured mushroom fungus is stimulated to re-form the fruiting body, and the fruiting body is allowed to grow. Repeat the process of harvesting. In the cultivation of such mushrooms, it is important to prevent various bacteria from invading and spreading in the culture medium during the cultivation of the mushrooms, particularly in the pre-culture until the mushrooms become dominant in the culture medium. It is important to promote healthy and sufficient cultivation of E. coli, and it is also important to promote it by simple operation. If healthy and sufficient cultivation of mushroom fungi can be promoted, as a result, many fruit bodies of healthy mushrooms can be harvested.
The present invention relates to a method for cultivating mushrooms which can promote healthy and sufficient cultivation of mushrooms having undergone the above-mentioned preliminary culture by a simple operation.

[0002]

2. Description of the Related Art Conventionally, as a method for cultivating mushrooms, it is necessary to inoculate a sterilized culture medium in a culture bag with a mushroom seed and to seal an upper opening of the culture bag through a sterilization filter. If there is, store the sealed culture bag in another air-permeable container, and after sufficiently cultivating the mushroom fungus in the culture medium, stimulate the fully cultured mushroom fungus, for example, by applying a low-temperature stimulus to the fruiting body. , And the fruiting body is grown (enlarged) and harvested (Japanese Patent Application Laid-Open No. 7-184474). Usually, the medium is removed from the culture bag before and after stimulating the well-cultured mushroom fungus, and thus the mushroom fruiting bodies are grown and harvested on the stripped medium. Thus, after harvesting the first mushroom fruit body, the culture medium was left naked, the mushrooms remaining in the culture medium were sufficiently re-cultured, and then stimulated to the fully re-cultured mushrooms. The operation of reshaping the body, growing and harvesting the fruiting body was repeated several times,
Harvesting mushroom fruit bodies since the first time.

However, the above-mentioned conventional method has the following disadvantages. 1) The process of cultivating the inoculum after inoculation in a sterilized medium is carried out in the pre-culture until the mushroom is predominantly cultured in the medium, and even in the case where the mushroom is more preferentially cultivated. It is roughly divided into late culture until it is sufficiently cultured to a good degree. In the conventional method, since the above-mentioned initial culture and late culture are performed in a culture bag sealed with a sterilization filter, it is difficult to sufficiently culture mushrooms, especially in the latter culture. The form of the fruit body of the mushroom harvested the second time is poor, and the yield is also poor. In the first-stage culture, it is necessary to culture the mushrooms in a culture bag sealed with a sterilization filter in order to prevent invasion and spread of various bacteria in the culture medium. Since this is also performed in the same culture bag sealed through a sterilization filter, the culture medium becomes overly moist and ventilation to the culture medium becomes poor, and it is difficult to sufficiently culture the mushroom bacteria in the culture medium. is there. 2) In order to sufficiently recultivate the mushroom fungus in the medium after the first harvest of the fruit body of the mushroom, it is necessary to prevent the invasion of various germs in the medium and to keep the medium in a suitable moisturizing condition and aeration. However, in the conventional method, mushroom fungi are re-cultured while the medium is left naked, so that it is inevitable that various bacteria spread on some of the medium, and for example, to keep the medium moist. Since the manual work of sprinkling water on the culture medium or immersing the culture medium in water is performed, such work is extremely troublesome, and in many cases, the work for spreading various bacteria, moisturizing and ventilating is inappropriate. For this reason, it is difficult to sufficiently reculture the mushroom fungus in the culture medium, and as a result, the morphology of the fruit body of the mushroom harvested after the second harvest is inferior, and the yield is also inferior.

[0004]

The problem to be solved by the present invention is that, in the conventional method, the form of the fruiting body of the harvested mushroom is inferior, and the yield is also inferior.

[0005]

Means for Solving the Problems As a result of trial and error in order to solve the above-mentioned problems, the present inventor has found that in order to improve the form and the yield of mushroom fruit bodies collected from the first harvest, It is preferable that the culture medium in which the mushroom fungus is predominantly cultured by culturing is stored in a specific cultivation bag and cultured later, and the form and yield of the fruit body of the mushroom harvested after the second harvest are improved. In order to do this, it has been found that it is preferable to store the medium after harvesting the first mushroom in a specific cultivation bag and recultivate the medium.

That is, the present invention relates to a method for cultivating mushrooms, which comprises the following first to third operations. First work: Inoculation of a mushroom inoculum into a sterilized medium, and culture of mushrooms predominantly in the medium while preventing various bacterial contaminations. Second work: The medium in which mushrooms are predominantly cultured is 1 wall thick.
A hole having a diameter of 0.10 to 0.45 mm in a portion of 0 to 50 μm and forming an upper space in the bag has a surface porosity of 0.001 to 0.001.
In a cultivation bag made of synthetic resin formed in a large number at a ratio of 0.02%, the cultivation bag is housed in a state where an upper space in the bag corresponding to 1 to 4 times the volume of the medium is formed, and the mushroom bacterium is sufficiently contained in the medium. 3rd operation: stimulating well-cultured mushroom fungi to form fruiting bodies, developing and harvesting the fruiting bodies. The present invention includes the following first to fourth operations. The present invention relates to a method for cultivating mushrooms. First work: Inoculation of a mushroom inoculum into a sterilized medium, and sufficient culture of the mushroom fungus in the medium while preventing various bacterial contamination. Second work: Stimulation of the sufficiently cultured mushroom fungus to induce fruiting bodies Forming, growing the fruiting body and harvesting. Third work: The medium after harvesting the fruiting body is put into a medium having a thickness of 10 to 10 cm.
It is 50 μm and has a diameter of 0.
A hole of 10 to 0.45 mm has a surface porosity of 0.001 to 0.0
In a cultivation bag made of synthetic resin formed at a large ratio of 2%,
The medium is stored in a state in which an upper space in the bag corresponding to 1 to 4 times the volume of the medium is formed, and the medium is sufficiently re-cultured with the mushroom bacteria. The fourth operation: Stimulates the fully re-cultured mushroom bacteria. Work to regenerate the fruiting body and grow and harvest the fruiting body

[0007] In order to improve the form and yield of the fruit body of mushrooms that are harvested from the first time, also in the present invention, the pre-culture until the mushroom fungus is predominantly cultured is performed in the same manner as the conventional method. Generally, in the culture of mushrooms, a culture bag made of a synthetic resin such as polyethylene or polypropylene is used, and the culture bag is filled with a medium, sterilized, and cooled. Next, inoculate the sterilized medium in the culture bag with the inoculum of mushrooms,
The upper opening of the culture bag is sealed. At the time of sealing, as described above, a sterilizing filter is used, and furthermore, a container having a specific air permeability is used. The temperature is 5 to 35 ° C., preferably 17 to 25 ° C., the humidity is 30 to 90%, preferably 55 to 65%, the peripheral air flow is 0.2 to 2.0 m / sec, preferably 0.5 to 1. Culture under conditions of 5 m / sec. The culture bag used for the pre-culture and the method of sealing the opening thereof may be in accordance with the conventional method, and under the above conditions,
Usually, the mushroom fungi can be predominantly cultured in the medium around 75 days before and after.

[0008] In the present invention, a medium in which mushrooms are predominantly cultured by pre-culture is stored in a cultivation bag, and is subsequently cultured under the same conditions as in the pre-culture. Under the same conditions as in the first-stage culture, mushrooms can be sufficiently cultured in the medium usually around 75 days ago. Immediately put the cultured medium in a cultivation bag,
Although the upper opening of the cultivation bag is sealed, a sterilizing filter is not used at the time of sealing, and a container is not used after the sealing. When the upper opening of the cultivation bag is sealed, a space is formed above the culture medium in the cultivation bag. In the present invention, the portion forming the upper space in the bag has a diameter of 0.10-0. 45mm,
Preferably, a hole of 0.15 to 0.30 mm has a surface porosity of 0.1 mm.
001 to 0.02%, preferably 0.003 to 0.00
A cultivation bag formed in large numbers at a rate of 8% is used. Such a hole can be formed by inserting a needle into a cultivation bag made of a synthetic resin such as polyethylene or polypropylene with a needle, punching out with a punch, or opening with a laser beam or a chemical.

If a large number of the above holes are formed in the portion forming the upper space in the bag, various bacteria and air enter into the upper space in the bag from the holes during culture (during the latter stage of culture or during reculturing described later). I do. However, under the above-described culture conditions, the inside of the bag is kept at an elevated pressure due to the generated CO ₂ and H ₂ O, so that even if the bacteria enter the upper space in the bag once, the bacteria remain in the upper space. It does not attach to the lower medium and does not spread, and is again discharged out of the bag through the hole. Even if various bacteria enter the upper space in the bag and a part of the bacteria adhere to the medium, there is no problem because the mushrooms are already predominant in the medium. Bacteria that have entered the upper space in the bag are discharged out of the bag again by using the lifting pressure, thereby preventing the bacteria from attaching to the culture medium and spreading, while reducing oxygen in the air introduced into the upper space in the bag. In order to promote appropriate and healthy cultivation of the mushroom fungi while encouraging the medium to be appropriately moisturized while being taken into the medium by the diffusion, the diameter of the hole forming the upper space in the bag and the surface porosity of the surface must be determined. It is important to limit as described above. In the present invention, the diameter of the hole means the outer diameter of the piercing portion of the used needle when it is a hole pierced with a needle, and when it is a hole punched with a punch. Means the inner diameter of the tip of the punch used. Further, the surface porosity means the ratio (%) of the calculated total surface area of the holes to the surface area of the cultivation bag in the portion forming the upper space in the bag.

The bacteria invading the upper space in the bag are discharged out of the bag again by using the lifting pressure, while the oxygen in the air introduced into the upper space in the bag is diffused into the medium, and the medium is appropriately cooled. The size of the upper space in the bag formed above the culture medium is also important in order to keep moisture moist. In the present invention, the upper opening of the cultivation bag is sealed in a state where an upper space in the bag corresponding to 1 to 4 times, preferably 1.5 to 3 times the volume of the medium is formed. In addition, various bacteria that have invaded the upper space in the bag are discharged to the outside of the bag again by using the lifting pressure, and while the oxygen in the air introduced into the upper space in the bag is diffused into the medium, the medium is appropriately moisturized. In order to achieve
It is also important to maintain the upper space in the bag as described above. In the present invention, in order to give the bag for cultivation after sealing the upper opening a corresponding independence and to hold the upper space in the bag, the thickness is 10 to 50 μm, preferably 30 to 40 μm made of synthetic resin. Use cultivation bags.

After the mushroom fungus is sufficiently cultured in the medium by the late stage culture, the sufficiently cultured mushroom fungus is stimulated to form fruiting bodies, and the fruiting bodies are grown and harvested. The first fruiting body is thus harvested, but the formation, development and harvesting of the fruiting body may be in accordance with conventional methods.

After the first harvest of the fruiting body, the medium is re-contained in the cultivation bag used for the first time, left in the cultivation room, and the mushroom fungus is sufficiently re-cultured in the medium. Stimulate the fully recultured mushroom fungus to regenerate the fruiting body, and grow and harvest the fruiting body. Thus, the second fruiting body is harvested, and thereafter, the same operation is repeated,
Harvest the third and subsequent fruiting bodies. When cultivation is performed according to the operation procedure described above, the form and amount of harvested mushroom body can be improved from the first time.

[0013] In the case of improving the morphology and yield of mushroom fruit bodies to be harvested from the second time onward, the present invention also provides culture of mushroom fungi, formation and development of fruit bodies until the first mushroom fruit body is harvested. The harvesting is performed in the same manner as in the conventional method. In the present invention, the culture medium after harvesting the first fruiting body is stored in the above-mentioned cultivation bag instead of reculturing the mushroom fungi in the culture medium while keeping the culture medium naked as in the conventional method. After allowing the mushrooms to be sufficiently re-cultured in the culture medium and then stimulating the fully re-cultured mushrooms, the fruiting bodies are reformed and the fruiting bodies are harvested. Thus, the second fruiting body is harvested.
Harvest the subsequent fruiting bodies. When cultivated according to the operation procedure described above, the form and yield of the fruit body of the mushroom harvested after the second time can be improved.

[0014]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention include the following 1) and 2). 1) A medium is put into a polyethylene culture bag having a wall thickness of 40 μm, sterilized by heating under pressure, and cooled. Next, the sterilized medium in the culture bag was inoculated with Shiitake bacterium, and the upper opening of the culture bag was sealed with a sterilization filter.
7-25 ° C, humidity 55-65%, peripheral airflow 0.5-1.
The culture is pre-cultured for 75 days under the condition of 5 m / sec, and the medium is predominantly cultured in the medium. The medium in which the Shiitake bacterium was predominantly cultured was immediately placed in a cultivation bag of a polyethylene bag having a wall thickness of 40 μm, and the periphery of the upper opening of the cultivation bag was bent four times while holding a rectangular flat aluminum piece. , And seal with clips. A cultivation bag having a volume several times that of the culture medium to be put into it and having a large number of holes formed by piercing the needle with a slightly upper portion is used, but is sealed with a clip as described above. An upper space in the bag equivalent to three times the volume of the sterilized medium is formed, and a hole having a diameter of 0.20 mm (a needle having an outer diameter of 0.20 mm at the piercing portion) is formed in a portion forming the upper space in the bag. The pierced hole) has a surface porosity of 0.
Many are formed at a rate of 006%. Then, cultivation is performed in the latter stage for 75 days under the same conditions as in the first cultivation, and the medium is sufficiently cultured. After applying a low-temperature stimulus of 7 to 10 ° C. for 3 days to the medium in which the Shiitake bacterium was sufficiently cultured to form fruiting bodies, the cultivation bag was removed, the upper opening edge was pulled down around the lower medium, and the culture medium was removed. With the upper surface open, the formed fruiting body is kept at a temperature of 17 to 25 ° C and a humidity of 70 to
80%, ambient air flow of 0.1 to 0.3 m / sec.
Grow and harvest for days. Thus, after harvesting the first fruit body of Shiitake mushroom, the upper opening periphery of the lowered cultivation bag is pulled up, the raised upper opening periphery is sealed, and the cultivation room is left for another 40 days in the cultivation room. Re-culture the bacteria thoroughly. Then, in the same manner as described above, the medium in which the Shiitake bacterium has been sufficiently re-cultured is subjected to low-temperature stimulation to regenerate the fruiting body, and the fruiting body is developed, and the second fruiting body of the shiitake mushroom is harvested. Thereafter, the same operation is repeated twice more.
Harvest shiitake fruiting bodies from the same medium a total of four times.

2) A medium is placed in a culture bag made of polypropylene having a thickness of 30 μm, sterilized by heating under pressure, and cooled. Next, the sterilized medium in the culture bag is inoculated with Shiitake bacterium, the upper opening of the culture bag is sealed with a sterilization filter, and the temperature is 17 to 25 ° C., the humidity is 55 to 65%, and the surrounding airflow is reduced. Cultured under a condition of 0.5 to 1.5 m / sec for 150 days,
Cultivate well the medium in the medium. After applying a low-temperature stimulus of 7 to 10 ° C. for 3 days to a medium in which Shiitake bacterium was sufficiently cultured to form fruiting bodies, the medium was taken out of the culture bag, and the formed fruiting bodies were heated at a temperature of 17 to 25 ° C. and a humidity of 70 to 70 ° C. 80%,
Harvest is performed by growing for 20 days under the condition of a peripheral air flow of 0.1 to 0.3 m / sec. After the first harvest of the fruit body of Shiitake mushrooms, the culture medium is placed in a cultivation bag, and the upper opening edge of the cultivation bag is bent four times while holding a rectangular flat aluminum piece, and sealed with a clip. Stop. A cultivation bag having a volume several times that of the culture medium to be put into it and having a large number of holes formed by piercing the needle with a slightly upper portion is used, but is sealed with a clip as described above. Thus, an upper space in the bag equivalent to twice the volume of the sterilized medium is formed, and a portion forming the upper space in the bag has a diameter of 0.2.
A large number of 5 mm holes (holes pierced with a needle having an outer diameter of 0.25 mm at the piercing portion) are formed at a surface porosity of 0.004%. Then, the cells are left in the cultivation room for another 40 days, and the medium is sufficiently re-cultured. Apply a low-temperature stimulus of 7 to 10 ° C to the medium in which
After giving the seedlings for days, the sealing of the cultivation bag is removed, the upper opening edge is pulled down around the lower culture medium, the upper surface of the culture medium is opened, and the formed fruiting body is heated at a temperature of 17 to 2 hours.
5 ° C, humidity 70-80%, ambient air flow 0.1-0.3m /
Grow and harvest for 20 days under conditions of seconds. Thus 2
After harvesting the fruit body of the Shiitake mushroom for the second time, the upper opening periphery of the lowered cultivation bag is pulled up, the pulled up upper opening periphery is sealed, and in the same manner as the above-described reculturing, the Shiitake bacterium is sufficiently provided in the medium. Re-culture. Then, in the same manner as described above, the medium in which the Shiitake bacterium has been sufficiently re-cultured is subjected to a low-temperature stimulation to regenerate the fruiting body, and the fruiting body is developed, and the third fruiting body of Shiitake mushroom is harvested. Hereafter, the same work is performed for one more time.
Repeat once and harvest the shiitake fruiting body from the same medium a total of four times.

[0016]

【Example】

Test Category 1 Example 1 A culture medium having a composition of 90% by weight of sawdust, 5% by weight of bran, 4% by weight of rice bran and 1% by weight of corn powder and a water content of 65% by weight was placed in a polyethylene culture bag having a thickness of 40 μm. 2) Put 2 kg, heat sterilize to a medium core temperature of 100 ° C., and cool. Next, 30 g of Shiitake fungi was inoculated into the sterilized medium in the culture bag, and the upper opening of the culture bag was sealed with a sterilization filter, and then the temperature was 17 to 25 ° C. and the humidity was 55 to 65%. The culture was pre-cultured for 75 days under the conditions of a peripheral air flow of 0.5 to 1.5 m / sec, and the medium was predominantly cultured in the medium. The medium in which the Shiitake bacterium was predominantly cultured was immediately placed in a cultivation bag of a polyethylene bag having a wall thickness of 40 μm, and the periphery of the upper opening of the cultivation bag was bent four times while holding a rectangular flat aluminum piece. , And sealed with clips. The cultivation bag used had a volume several times that of the medium to be put into it, and had a large number of holes formed by piercing a needle in the upper part, but sealed with clips as described above. In this state, an upper space in the bag equivalent to 3 times the volume of the sterilized medium is formed, and a hole having a diameter of 0.20 mm (a needle having an outer diameter of 0.20 mm at the piercing portion) is formed in a portion forming the upper space in the bag. Are formed at a rate of 0.006% on the surface. Fifty bags were prepared in the same manner, and these were cultivated in the latter stage for 75 days under the same conditions as in the first cultivation. After applying a low-temperature stimulus of 7 to 10 ° C. to the medium in which the Shiitake bacterium was sufficiently cultured for 3 days to form fruiting bodies, the sealing of the cultivation bag was removed, the upper periphery was pulled down around the lower medium, and the upper surface of the medium was removed. Is opened, and the formed fruit body is heated to a temperature of 17 to 25 ° C.
The plants were grown for 20 days under conditions of a humidity of 70 to 80% and an ambient air flow of 0.1 to 0.3 m / sec, and were harvested. Thus, after harvesting the first fruit body of Shiitake mushroom, the upper opening peripheral edge of the lowered cultivation bag is pulled up, the raised upper opening peripheral edge is sealed in the same manner as described above, and further left for 40 days in the cultivation room,
The mushrooms were re-cultured sufficiently in the medium. And in the same manner as described above, a low temperature stimulus is applied to the medium in which the Shiitake bacterium has been sufficiently re-cultured to regenerate fruiting bodies, and the fruiting bodies are allowed to grow,
A second harvest of shiitake mushroom was harvested. Thereafter, the same operation was repeated two more times, and a total of four times Shiitake mushrooms were harvested from the same medium. The morphology of the fruit bodies of Shiitake mushrooms harvested from the first to the fourth harvest were evaluated based on the following criteria.
The average yield (g) per medium and the total yield (g) were determined, and the results are shown in Table 1.

Criteria for evaluating the morphology of fruiting bodies A: Large shiitake mushrooms as a whole B: Medium intermediate shiitake mushrooms as a whole C: Small lower shiitake mushrooms as a whole

Example 2 In the same manner as in Example 1, the culture was cultivated for 75 days, and the medium was predominantly cultured with Shiitake bacterium. The culture was performed late for 75 days under the culture conditions, and the medium was also sufficiently cultured. After applying a low-temperature stimulus of 7 to 10 ° C. for 3 days to a medium in which Shiitake bacterium was sufficiently cultured to form fruiting bodies, the medium was removed from the culture bag, and the formed fruiting bodies were subjected to a temperature of 17 to 25 ° C. and a humidity of 7 ° C.
The plants were grown for 20 days under the conditions of 0 to 80% and a surrounding airflow of 0.1 to 0.3 m / sec and harvested. After harvesting the first fruit body of Shiitake mushroom, the culture medium was put in a cultivation bag and sealed in the same manner as in Example 1, and left in the cultivation room for another 40 days in the same manner as in the reculturing in Example 1. Then, the medium was sufficiently re-cultured in the medium. After applying a low-temperature stimulus of 7 to 10 ° C. to the medium in which the Shiitake bacterium was sufficiently re-cultured for 3 days to form fruiting bodies, the sealing of the cultivation bag was removed, and the periphery of the upper opening was pulled down around the lower medium. The fruiting body thus formed was grown for 20 days under conditions of a temperature of 17 to 25 ° C., a humidity of 70 to 80%, and a peripheral airflow of 0.1 to 0.3 m / sec, and was harvested. Thus, after harvesting the second fruit body of Shiitake mushroom, the upper opening periphery of the lowered cultivation bag is pulled up, the pulled up upper opening periphery is sealed in the same manner as above, and further left for 40 days in the cultivation room, The mushrooms were re-cultured sufficiently in the medium. Then, in the same manner as described above, a medium in which the Shiitake bacterium was sufficiently re-cultured was subjected to a low-temperature stimulation to regenerate the fruiting body, and the fruiting body was developed, and the third fruiting body of the shiitake mushroom was harvested. Thereafter, the same operation was repeated once more, and a total of four times the shiitake mushroom was harvested from the same medium. The morphology of the shiitake mushroom fruit bodies that were harvested the first to fourth times were evaluated based on the same criteria as in Example 1, and the yield was also determined. The results are shown in Table 1.

Comparative Example 1 In the same manner as in Example 2, the first-stage culture, the second-stage culture, the formation of the fruiting body, the growth and the harvesting were performed, and the first fruiting body of Shiitake mushroom was harvested. After harvesting the first shiitake fruit body,
While keeping the medium naked, the medium was repeatedly sprinkled with water in order to keep the medium moist, and further placed in a cultivation room for 40 days, and the medium was cultured sufficiently in the medium. The formation, development and harvesting of the fruiting body were performed in the same manner as above, and the second fruiting body of Shiitake was harvested. Thereafter, the same operation was repeated two more times, and a total of four times Shiitake mushrooms were harvested from the same medium. The first to fourth harvested shiitake fruit bodies were evaluated according to the same criteria as in Example 1, and the yield was also determined. The results are shown in Table 1.

[0020]

[Table 1]

Test Category 2 Examples 3 to 5 and Comparative Examples 2 and 3 Same as Example 1 except that the diameter of the hole forming the upper space in the bag for cultivation was changed as shown in Table 2. And then
Shiitake mushroom fruit bodies were harvested a total of four times from the same medium. The morphology of the shiitake mushroom fruit bodies that were harvested the first to fourth times were evaluated based on the same criteria as in Example 1, and the yield was also determined. The results are shown in Table 2.

[0022]

[Table 2]

Test Category 3 Examples 6 to 8 and Comparative Examples 4 and 5 Except that the surface porosity of the hole forming the upper space in the cultivation bag was changed as shown in Table 3 In the same manner as in 1, the shiitake mushroom fruit bodies were harvested a total of four times from the same medium. The morphology of the shiitake mushroom fruit bodies that were harvested the first to fourth times were evaluated based on the same criteria as in Example 1, and the yield was also determined. The results are shown in Table 3.

[0024]

[Table 3]

Test Category 4 Examples 9 to 11 and Comparative Examples 6, 7 A total of four times from the same medium in the same manner as in Example 1 except that the thickness of the cultivation bag was changed as shown in Table 4. Harvested shiitake mushrooms. The morphology of the shiitake mushroom fruit bodies that were harvested the first to fourth times were evaluated based on the same criteria as in Example 1, and the yield was also determined. The results are shown in Table 4.

[0026]

[Table 4]

Test Category 5 Examples 12 to 14 and Comparative Examples 8 and 9 In the same manner as in Example 1 except that the capacity ratio of the upper space in the bag to the culture medium of the cultivation bag was changed as shown in Table 5, Shiitake mushroom fruit bodies were harvested a total of four times from the same medium. 1
The morphology of the shiitake mushroom fruit bodies that were harvested the fourth to fourth times were evaluated based on the same criteria as in Example 1, and the yield was also determined. Table 5 shows the results.

[0028]

[Table 5]

[0029]

As is clear from the above, the present invention described above can promote healthy and sufficient cultivation of mushroom fungi by a simple operation, and as a result, improve the form and yield of mushroom fruit body. There is an effect that can be.

Claims (6)

[Claims] 1. A method for cultivating a mushroom, which comprises the following first to third operations. First work: Inoculation of a mushroom inoculum into a sterilized medium, and culture of mushrooms predominantly in the medium while preventing various bacterial contaminations. Second work: The medium in which mushrooms are predominantly cultured is 1 wall thick.
A hole having a diameter of 0.10 to 0.45 mm in a portion of 0 to 50 μm and forming an upper space in the bag has a surface porosity of 0.001 to 0.001.
In a cultivation bag made of synthetic resin formed in a large number at a ratio of 0.02%, the cultivation bag is housed in a state where an upper space in the bag corresponding to 1 to 4 times the volume of the medium is formed, and the mushroom bacterium is sufficiently contained in the medium. 3rd work: Stimulation of well-cultured mushroom fungi to form fruiting bodies, and developing and harvesting the fruiting bodies 2. A method for cultivating a mushroom, comprising the following first to fifth operations. First work: Inoculation of a mushroom inoculum into a sterilized medium, and culture of mushrooms predominantly in the medium while preventing various bacterial contaminations. Second work: The medium in which mushrooms are predominantly cultured is 1 wall thick.
A hole having a diameter of 0.10 to 0.45 mm in a portion of 0 to 50 μm and forming an upper space in the bag has a surface porosity of 0.001 to 0.001.
In a cultivation bag made of synthetic resin formed in a large number at a ratio of 0.02%, the cultivation bag is housed in a state where an upper space in the bag corresponding to 1 to 4 times the volume of the medium is formed, and the mushroom bacterium is sufficiently contained in the medium. 3rd operation: stimulating well-cultured mushroom fungi to form fruiting bodies, developing and harvesting the fruiting bodies. 4th operation: culturing the medium after harvesting the fruiting bodies in the second step. Work to store in a cultivation bag in the same manner as in the operation and sufficiently recultivate the mushroom fungus in the culture medium. Fifth work: Stimulate the fully recultured mushroom fungus to re-form the fruiting body, Growing and harvesting 3. The method for cultivating a mushroom according to claim 2, wherein the fourth operation and the fifth operation are repeated. 4. A method for cultivating a mushroom, comprising the following first to fourth operations. First work: Inoculation of a mushroom inoculum into a sterilized medium, and sufficient culture of the mushroom fungus in the medium while preventing various bacterial contamination. Second work: Stimulation of the sufficiently cultured mushroom fungus to induce fruiting bodies Forming, growing the fruiting body and harvesting. Third work: The medium after harvesting the fruiting body is put into a medium having a thickness of 10 to 10 cm.
It is 50 μm and has a diameter of 0.
A hole of 10 to 0.45 mm has a surface porosity of 0.001 to 0.0
In a cultivation bag made of synthetic resin formed at a large ratio of 2%,
The medium is stored in a state in which an upper space in the bag corresponding to 1 to 4 times the volume of the medium is formed, and the medium is sufficiently re-cultured with the mushroom bacteria. The fourth operation: Stimulates the fully re-cultured mushroom bacteria. Work to regenerate the fruiting body and grow and harvest the fruiting body 5. The method for cultivating a mushroom according to claim 4, wherein the third operation and the fourth operation are repeated. 6. A medium having a thickness of 30 to 40 μm and a diameter of 0.15 to 0.30 mm in a portion forming an upper space in the bag.
In a cultivation bag made of synthetic resin in which a large number of pores are formed at a surface porosity of 0.003 to 0.008%.
The method for cultivating mushrooms according to claim 1, 2, 3, 4, or 5, wherein the mushrooms are stored in a state in which an upper space in a bag corresponding to up to 3 times the volume is formed.